(Linear) (Six-base) MAPSORT of: KIEE3892.seq  Check: 9266  from: 1  to: 459



KIEE3892.orf

 With 182 enzymes: SgfI * 

                        August 4, 2021 20:20  ..

AvaI C'yCGr_G

Cuts at:      0     437     459 
   Size:        437      22
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       1      35      64      98     459 
   Size:          1      34      29      34     361

  Fragments arranged by size: 

                 361      34      34      29       1
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     459 
   Size:        295     164
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      21     459 
   Size:         21     438
 
BsaHI Gr'CG_yC

Cuts at:      0      14     459 
   Size:         14     445
 
BsiEI CG_ry'CG

Cuts at:      0     261     459 
   Size:        261     198
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       2     459 
   Size:          2     457
 
BspHI T'CATG_A

Cuts at:      0     170     459 
   Size:        170     289
 
BstEII G'GTnAC_C

Cuts at:      0     192     459 
   Size:        192     267
 
Bsu36I CC'TnA_GG

Cuts at:      0     412     459 
   Size:        412      47
 
BtgI C'CryG_G

Cuts at:      0     237     459 
   Size:        237     222
 
EarI CTCTTCn'nnn_

Cuts at:      0     391     459 
   Size:        391      68
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     459 
   Size:        295     164
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      21     459 
   Size:         21     438
 
EcoO109I rG'GnC_Cy

Cuts at:      0     330     459 
   Size:        330     129
 
MslI CAynn'nnrTG

Cuts at:      0     242     459 
   Size:        242     217
 
MspA1I CmG'CkG

Cuts at:      0     383     459 
   Size:        383      76
 
NspI r_CATG'y

Cuts at:      0     351     455     459 
   Size:        351     104       4

  Fragments arranged by size: 

                 351     104       4
 
PasI CC'CwG_GG

Cuts at:      0      32     459 
   Size:         32     427
 
PpuMI rG'GwC_Cy

Cuts at:      0     330     459 
   Size:        330     129
 
PstI C_TGCA'G

Cuts at:      0     231     459 
   Size:        231     228
 
PvuI CG_AT'CG

Cuts at:      0     261     459 
   Size:        261     198
 
PvuII CAG'CTG

Cuts at:      0     383     459 
   Size:        383      76
 
SfcI C'TryA_G

Cuts at:      0     227     459 
   Size:        227     232
 
StuI AGG'CCT

Cuts at:      0      70     459 
   Size:         70     389
 

 Enzymes that do cut:

     AvaI     BcgI     BpmI     BsaI    BsaHI    BsiEI    BsmBI    BspHI
   BstEII   Bsu36I     BtgI     EarI  Eco57MI    EcoNI EcoO109I     MslI
   MspA1I     NspI     PasI    PpuMI     PstI     PvuI    PvuII     SfcI
     StuI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI