##################################
# NO CUT ENZYMES (   50 enzymes) #
##################################

AatII       AgeI        AhdI        AscI        AseI        
AsnI        AspEI       AvrII       BclI        BlnI        
BsaBI       BsiWI       BspDI       BspLU11I    Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraI        
Eam1105I    EcoRI       EcoT22I     FbaI        FseI        
HincII      HindII      HpaI        MamI        MfeI        
MluI        MunI        NdeI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PshBI       
PvuI        RsrII       SalI        SfuI        SgrAI       
SnaBI       SpeI        SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AccI        11003  GTCTAC               GT^[AC][GT]AC
AclI         7766  AACGTT               AA^CGTT
AcsI         8172  GAATTT               [GA]^AATT[CT]
Aor51HI       800  AGCGCT               AGC^GCT
ApoI         8172  GAATTT               [GA]^AATT[CT]
AspI         7120  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BciVI        3872  GTATCC               GTATCC(6/5)
Bsp1407I      239  TGTACA               T^GTACA
BsrGI         239  TGTACA               T^GTACA
BstEII       6841  GGTGACC              G^GT[ACGT]ACC
BstPI        6841  GGTGACC              G^GT[ACGT]ACC
DrdI         5448  GACCGCCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      800  AGCGCT               AGC^GCT
EcoO65I      6841  GGTGACC              G^GT[ACGT]ACC
EcoRV        2888  GATATC               GAT^ATC
PaeR7I       6662  CTCGAG               C^TCGAG
PflFI        7120  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         813  GACCTACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     7766  AACGTT               AA^CGTT
SspBI         239  TGTACA               T^GTACA
Tth111I      7120  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         6662  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

Acc65I       7064  GGTACC               G^GTACC
            10594  GGTACC              
AflII        1324  CTTAAG               C^TTAAG
            10830  CTTAAG              
Asp718I      7064  GGTACC               G^GTACC
            10594  GGTACC              
BfrI         1324  CTTAAG               C^TTAAG
            10830  CTTAAG              
BsmI         1989  GAATGC               GAATGC(1/-1)
             1039  GCATTC              
BssHII       2382  GCGCGC               G^CGCGC
             4030  GCGCGC              
HindIII      4205  AAGCTT               A^AGCTT
            10270  AAGCTT              
KpnI         7064  GGTACC               GGTAC^C
            10594  GGTACC              
KspI         7917  CCGCGG               CCGC^GG
            10683  CCGCGG              
NaeI         1662  GCCGGC               GCC^GGC
             5369  GCCGGC              
NgoMIV       1662  GCCGGC               G^CCGGC
             5369  GCCGGC              
NheI         9228  GCTAGC               G^CTAGC
             9978  GCTAGC              
SacII        7917  CCGCGG               CCGC^GG
            10683  CCGCGG              
ScaI         2246  AGTACT               AGT^ACT
             4889  AGTACT              
SexAI        6095  ACCAGGT              A^CC[AT]GGT
             8105  ACCTGGT             
TfiI         6261  GATTC                G^A[AT]TC
             7137  GATTC               
Tsp509I      7442  AATT                 ^AATT
             8173  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  169 enzymes) #
#########################################

AccII       AccIII      AciI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       Asp700I     AspHI       
AvaI        AvaII       AviII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BcnI        BfaI        
BglI        BglII       BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspEI       
BspHI       BspMI       BsrBI       BsrDI       BsrFI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MroI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NotI        NspI        PflMI       PleI        PmaCI       
PmlI        PpuMI       PstI        PvuII       RcaI        
RsaI        SacI        SapI        Sau3AI      Sau96I      
SbfI        ScrFI       SfaNI       SfcI        SfiI        
SfoI        SmaI        SmlI        SphI        Sse8387I    
StuI        StyI        TaqI        Tru9I       TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI        XmnI