(Circular) (Six-base) MAPSORT of: pFN21AE2693.seq Check: 2775 from: 1 to: 15727 pFN21AE2693 15727 bp With 182 enzymes: SgfI * August 25, 2017 13:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3334 3577 5114 6718 7894 9960 10803 10962 12648 Size: 243 1537 1604 1176 2066 843 159 1686 Cuts at: 12648 3334 Size: 6413 Fragments arranged by size: 6413 2066 1686 1604 1537 1176 843 243 159 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 13537 278 Size: 53 83 186 936 12001 2468 Fragments arranged by size: 12001 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 13009 13070 1937 Size: 11072 61 4594 Fragments arranged by size: 11072 4594 61 Acc65I G'GTAC_C Cuts at: 9069 12599 13048 9069 Size: 3530 449 11748 Fragments arranged by size: 11748 3530 449 AclI AA'CG_TT Cuts at: 1563 9772 13854 14227 1563 Size: 8209 4082 373 3063 Fragments arranged by size: 8209 4082 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2885 3545 3989 4988 5276 5603 7964 8309 Size: 1253 660 444 999 288 327 2361 345 Cuts at: 8309 8360 8540 8579 8613 9616 9991 10604 10684 Size: 51 180 39 34 1003 375 613 80 Cuts at: 10684 11118 12356 12509 13790 15174 15442 1632 Size: 434 1238 153 1281 1384 268 1917 Fragments arranged by size: 2361 1917 1384 1281 1253 1238 1003 999 660 613 444 434 375 345 327 288 268 180 153 80 51 39 34 AfeI AGC'GCT Cuts at: 2807 2807 Size: 15727 AflII C'TTAA_G Cuts at: 820 1017 3329 12835 820 Size: 197 2312 9506 3712 Fragments arranged by size: 9506 3712 2312 197 AflIII A'CryG_T Cuts at: 1203 6106 9103 9105 14626 15603 1203 Size: 4903 2997 2 5521 977 1327 Fragments arranged by size: 5521 4903 2997 1327 977 2 AgeI A'CCGG_T Cuts at: 14558 14558 Size: 15727 AhdI GACnn_n'nnGTC Cuts at: 14456 14456 Size: 15727 AleI CACnn'nnGTG Cuts at: 1166 2591 3821 7856 9936 1166 Size: 1425 1230 4035 2080 6957 Fragments arranged by size: 6957 4035 2080 1425 1230 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2733 5036 5862 7874 12267 1184 Size: 1549 2303 826 2012 4393 4644 Fragments arranged by size: 4644 4393 2303 2012 1549 826 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2070 2102 12450 12482 2070 Size: 32 10348 32 5315 Fragments arranged by size: 10348 5315 32 32 AlwNI CAG_nnn'CTG Cuts at: 2749 2767 3037 3227 3244 3409 3460 3697 3874 Size: 18 270 190 17 165 51 237 177 Cuts at: 3874 3919 4716 5221 5251 5344 5422 5485 5581 Size: 45 797 505 30 93 78 63 96 Cuts at: 5581 5614 5935 6355 6373 6814 6868 7447 7645 Size: 33 321 420 18 441 54 579 198 Cuts at: 7645 7687 7717 7900 8518 8692 8701 8755 8863 Size: 42 30 183 618 174 9 54 108 Cuts at: 8863 9172 9208 9469 9610 9655 9676 9730 10222 Size: 309 36 261 141 45 21 54 492 Cuts at: 10222 10243 10459 10777 10816 10933 11017 11413 11473 Size: 21 216 318 39 117 84 396 60 Cuts at: 11473 11669 11932 11980 12010 12121 12142 12196 12217 Size: 196 263 48 30 111 21 54 21 Cuts at: 12217 12289 12487 12640 12675 12934 13470 15042 2749 Size: 72 198 153 35 259 536 1572 3434 Fragments arranged by size: 3434 1572 797 618 579 536 505 492 441 420 396 321 318 309 270 263 261 259 237 216 198 198 196 190 183 177 174 165 153 141 117 111 108 96 93 84 78 72 63 60 54 54 54 54 51 48 45 45 42 39 36 35 33 30 30 30 21 21 21 21 18 18 17 9 ApaI G_GGCC'C Cuts at: 3895 3904 4512 4654 4675 5290 6328 6862 7816 Size: 9 608 142 21 615 1038 534 954 Cuts at: 7816 8512 9382 12235 3895 Size: 696 870 2853 7387 Fragments arranged by size: 7387 2853 1038 954 870 696 615 608 534 142 21 9 ApaLI G'TGCA_C Cuts at: 2339 9242 11529 12979 13784 14940 2339 Size: 6903 2287 1450 805 1156 3126 Fragments arranged by size: 6903 3126 2287 1450 1156 805 ApoI r'AATT_y Cuts at: 1493 10177 13036 13262 13414 15523 1493 Size: 8684 2859 226 152 2109 1697 Fragments arranged by size: 8684 2859 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 14281 160 Size: 872 13249 1606 Fragments arranged by size: 13249 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 15727 AvaI C'yCGr_G Cuts at: 1870 1943 2028 2039 2317 2774 4151 4200 4443 Size: 73 85 11 278 457 1377 49 243 Cuts at: 4443 5790 7292 7488 7986 8358 8667 9114 9308 Size: 1347 1502 196 498 372 309 447 194 Cuts at: 9308 9947 10851 10920 11963 12494 13052 1870 Size: 639 904 69 1043 531 558 4545 Fragments arranged by size: 4545 1502 1377 1347 1043 904 639 558 531 498 457 447 372 309 278 243 196 194 85 73 69 49 11 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 9092 9125 9092 Size: 33 15694 BamHI G'GATC_C Cuts at: 4147 8496 8814 11039 13057 13424 4147 Size: 4349 318 2225 2018 367 6450 Fragments arranged by size: 6450 4349 2225 2018 367 318 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2052 2389 2562 3815 4031 Size: 325 217 627 265 337 173 1253 216 Cuts at: 4031 4359 5292 5539 6384 6665 6774 7664 8352 Size: 328 933 247 845 281 109 890 688 Cuts at: 8352 9069 9624 9942 9972 10791 11513 11769 12599 Size: 717 555 318 30 819 722 256 830 Cuts at: 12599 13048 14503 618 Size: 449 1455 1842 Fragments arranged by size: 1842 1455 1253 933 890 845 830 819 722 717 688 627 555 449 337 328 325 318 281 265 256 247 217 216 173 109 30 BanII G_rGCy'C Cuts at: 1391 2037 2292 2368 3367 3766 3895 3904 4481 Size: 646 255 76 999 399 129 9 577 Cuts at: 4481 4512 4654 4675 4728 5096 5290 6027 6249 Size: 31 142 21 53 368 194 737 222 Cuts at: 6249 6328 6338 6603 6699 6862 7046 7816 8083 Size: 79 10 265 96 163 184 770 267 Cuts at: 8083 8243 8512 9382 9478 9494 9755 9843 10047 Size: 160 269 870 96 16 261 88 204 Cuts at: 10047 10419 10441 10479 10647 11028 11676 12235 12468 Size: 372 22 38 168 381 648 559 233 Cuts at: 12468 12787 12910 12941 13046 1391 Size: 319 123 31 105 4072 Fragments arranged by size: 4072 999 870 770 737 648 646 577 559 399 381 372 368 319 269 267 265 261 255 233 222 204 194 184 168 163 160 142 129 123 105 96 96 88 79 76 53 38 31 31 22 21 16 10 9 BbeI G_GCGC'C Cuts at: 4035 4363 5296 8356 11773 4035 Size: 328 933 3060 3417 7989 Fragments arranged by size: 7989 3417 3060 933 328 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2183 2197 2579 5141 8275 8348 9798 Size: 971 284 14 382 2562 3134 73 1450 Cuts at: 9798 11702 928 Size: 1904 4953 Fragments arranged by size: 4953 3134 2562 1904 1450 971 382 284 73 14 BbvCI CC'TCA_GC Cuts at: 4062 4118 4355 4857 5426 7221 8487 10097 10533 Size: 56 237 502 569 1795 1266 1610 436 Cuts at: 10533 12033 12300 4062 Size: 1500 267 7489 Fragments arranged by size: 7489 1795 1610 1500 1266 569 502 436 267 237 56 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8249 8283 11234 11268 12260 12294 13918 Size: 34 6312 34 2951 34 992 34 1624 Cuts at: 13918 13952 1903 Size: 34 3678 Fragments arranged by size: 6312 3678 2951 1624 992 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5888 13619 14829 5888 Size: 7731 1210 6786 Fragments arranged by size: 7731 6786 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 15727 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2223 3079 3104 Size: 107 122 71 631 605 551 856 25 Cuts at: 3104 3380 3899 4397 4536 5285 6428 6446 6470 Size: 276 519 498 139 749 1143 18 24 Cuts at: 6470 7877 8588 10271 10367 10529 10673 11108 11838 Size: 1407 711 1683 96 162 144 435 730 Cuts at: 11838 12311 12813 14338 136 Size: 473 502 1525 1525 Fragments arranged by size: 1683 1525 1525 1407 1143 856 749 730 711 631 605 551 519 502 498 473 435 276 162 144 139 122 107 96 71 25 24 18 BglII A'GATC_T Cuts at: 2481 4093 7537 9187 15722 2481 Size: 1612 3444 1650 6535 2486 Fragments arranged by size: 6535 3444 2486 1650 1612 BlpI GC'TnA_GC Cuts at: 2739 2760 3611 3783 4572 4875 5373 5415 9677 Size: 21 851 172 789 303 498 42 4262 Cuts at: 9677 11052 11670 12051 13144 2739 Size: 1375 618 381 1093 5322 Fragments arranged by size: 5322 4262 1375 1093 851 789 618 498 381 303 172 42 21 Bme1580I G_kGCm'C Cuts at: 1790 2055 2343 3818 3895 3904 4512 4654 4675 Size: 265 288 1475 77 9 608 142 21 Cuts at: 4675 5290 5544 5812 6328 6670 6777 6862 7147 Size: 615 254 268 516 342 107 85 285 Cuts at: 7147 7410 7667 7816 7831 7957 8122 8512 9246 Size: 263 257 149 15 126 165 390 734 Cuts at: 9246 9382 9627 9945 9964 10487 10868 10896 11301 Size: 136 245 318 19 523 381 28 405 Cuts at: 11301 11533 11995 12235 12354 12983 13788 14944 1790 Size: 232 462 240 119 629 805 1156 2573 Fragments arranged by size: 2573 1475 1156 805 734 629 615 608 523 516 462 405 390 381 342 318 288 285 268 265 263 257 254 245 240 232 165 149 142 136 126 119 107 85 77 28 21 19 15 9 BmgBI CAC'GTC Cuts at: 1318 2782 5059 5722 12040 1318 Size: 1464 2277 663 6318 5005 Fragments arranged by size: 6318 5005 2277 1464 663 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2088 2926 3190 Size: 416 53 83 399 24 671 838 264 Cuts at: 3190 6835 6856 8722 9609 10878 11846 13458 14416 Size: 3645 21 1866 887 1269 968 1612 958 Cuts at: 14416 442 Size: 1753 Fragments arranged by size: 3645 1866 1753 1612 1269 968 958 887 838 671 416 399 264 83 53 24 21 BmtI G_CTAG'C Cuts at: 1056 11237 11987 1056 Size: 10181 750 4796 Fragments arranged by size: 10181 4796 750 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2311 3655 3820 5078 5235 8663 8682 9513 9580 Size: 1344 165 1258 157 3428 19 831 67 Cuts at: 9580 2311 Size: 8458 Fragments arranged by size: 8458 3428 1344 1258 831 165 157 67 19 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2306 2369 2641 2891 3011 3233 3440 3671 Size: 1067 63 272 250 120 222 207 231 Cuts at: 3671 3758 3929 4178 4328 4394 4436 4467 4534 Size: 87 171 249 150 66 42 31 67 Cuts at: 4534 4592 4712 4760 4811 4979 5051 5093 5168 Size: 58 120 48 51 168 72 42 75 Cuts at: 5168 5468 5648 5885 5930 5936 6233 6329 6346 Size: 300 180 237 45 6 297 96 17 Cuts at: 6346 6416 6530 6683 6857 6938 7060 7202 7637 Size: 70 114 153 174 81 122 142 435 Cuts at: 7637 7667 7712 7841 8000 8207 8777 8798 8885 Size: 30 45 129 159 207 570 21 87 Cuts at: 8885 9212 9251 9413 9752 9887 9899 9929 10058 Size: 327 39 162 339 135 12 30 129 Cuts at: 10058 10310 10337 10421 10433 10520 10691 10838 10955 Size: 252 27 84 12 87 171 147 117 Cuts at: 10955 11051 11147 11219 11354 11435 11540 11660 11795 Size: 96 96 72 135 81 105 120 135 Cuts at: 11795 12239 12311 12652 14387 1239 Size: 444 72 341 1735 2579 Fragments arranged by size: 2579 1735 1067 570 444 435 341 339 327 300 297 272 252 250 249 237 231 222 207 207 180 174 171 171 168 162 159 153 150 147 142 135 135 135 129 129 122 120 120 120 117 114 105 96 96 96 87 87 87 84 81 81 75 72 72 72 70 67 66 63 58 51 48 45 45 42 42 39 31 30 30 27 21 17 12 12 6 Bpu10I CC'TnA_GC Cuts at: 4062 4118 4355 4475 4857 5426 5735 5774 6587 Size: 56 237 120 382 569 309 39 813 Cuts at: 6587 7098 7221 8487 10097 10533 10998 12033 12300 Size: 511 123 1266 1610 436 465 1035 267 Cuts at: 12300 4062 Size: 7489 Fragments arranged by size: 7489 1610 1266 1035 813 569 511 465 436 382 309 267 237 123 120 56 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3729 4645 6044 8960 13852 14717 15015 3729 Size: 916 1399 2916 4892 865 298 4441 Fragments arranged by size: 4892 4441 2916 1399 916 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2424 4340 6479 7508 8218 8686 11136 Size: 617 925 1916 2139 1029 710 468 2450 Cuts at: 11136 11336 14390 882 Size: 200 3054 2219 Fragments arranged by size: 3054 2450 2219 2139 1916 1029 925 710 617 468 200 BsaAI yAC'GTr Cuts at: 493 1204 4366 6109 9106 13518 493 Size: 711 3162 1743 2997 4412 2702 Fragments arranged by size: 4412 3162 2997 2702 1743 711 BsaBI GATnn'nnATC Cuts at: 15721 15721 Size: 15727 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4032 4360 4448 5293 Size: 53 83 186 936 2499 328 88 845 Cuts at: 5293 8353 10707 11358 11770 12014 13534 13916 14551 Size: 3060 2354 651 412 244 1520 382 635 Cuts at: 14551 275 Size: 1451 Fragments arranged by size: 3060 2499 2354 1520 1451 936 845 651 635 412 382 328 244 186 88 83 53 BsaWI w'CCGG_w Cuts at: 2235 6029 6501 9066 9369 14160 14558 14832 14979 Size: 3794 472 2565 303 4791 398 274 147 Cuts at: 14979 15390 2235 Size: 411 2572 Fragments arranged by size: 4791 3794 2572 2565 472 411 398 303 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2669 2699 2831 2861 3753 Size: 30 165 30 1266 30 132 30 892 Cuts at: 3753 3783 4301 4331 4715 4745 6142 6172 6344 Size: 30 518 30 384 30 1397 30 172 Cuts at: 6344 6374 7028 7058 7856 7886 7982 8012 8877 Size: 30 654 30 798 30 96 30 865 Cuts at: 8877 8907 8941 8971 11999 12029 12450 12480 12815 Size: 30 34 30 3028 30 421 30 335 Cuts at: 12815 12845 1178 Size: 30 4060 Fragments arranged by size: 4060 3028 1397 1266 892 865 798 654 518 421 384 335 172 165 132 96 34 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2522 2893 3668 3796 3965 3968 4433 Size: 841 495 371 775 128 169 3 465 Cuts at: 4433 4697 5054 5167 5207 5465 5923 5935 5938 Size: 264 357 113 40 258 458 12 3 Cuts at: 5938 6947 7067 7925 8204 8624 9239 9489 10055 Size: 1009 120 858 279 420 615 250 566 Cuts at: 10055 10139 10307 10676 11342 11578 12038 12236 1186 Size: 84 168 369 666 236 460 198 4677 Fragments arranged by size: 4677 1009 858 841 775 666 615 566 495 465 460 458 420 371 369 357 279 264 258 250 236 198 169 168 128 120 113 84 40 12 3 3 BseYI C'CCAG_C Cuts at: 2069 2632 2749 3201 3409 3504 3858 4567 5842 Size: 563 117 452 208 95 354 709 1275 Cuts at: 5842 6924 7815 8013 8017 8511 8685 10261 10297 Size: 1082 891 198 4 494 174 1576 36 Cuts at: 10297 10543 10861 10889 11007 11238 12079 12261 12318 Size: 246 318 28 118 231 841 182 57 Cuts at: 12318 14930 15426 2069 Size: 2612 496 2370 Fragments arranged by size: 2612 2370 1576 1275 1082 891 841 709 563 496 494 452 354 318 246 231 208 198 182 174 118 117 95 57 36 28 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2693 4790 5006 5032 5681 5832 6554 Size: 573 957 2097 216 26 649 151 722 Cuts at: 6554 7136 7289 7297 7730 7766 9221 9905 9914 Size: 582 153 8 433 36 1455 684 9 Cuts at: 9914 10751 10904 10946 11486 11900 11928 12365 1163 Size: 837 153 42 540 414 28 437 4525 Fragments arranged by size: 4525 2097 1455 957 837 722 684 649 582 573 540 437 433 414 216 153 153 151 42 36 28 26 9 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4341 4401 6944 8182 8919 13177 Size: 275 186 2340 60 2543 1238 737 4258 Cuts at: 13177 13938 14966 1540 Size: 761 1028 2301 Fragments arranged by size: 4258 2543 2340 2301 1238 1028 761 737 275 186 60 BsiHKAI G_wGCw'C Cuts at: 2037 2292 2343 2368 4728 5064 5096 5436 5780 Size: 255 51 25 2360 336 32 340 344 Cuts at: 5780 6249 6338 6603 6621 6699 7046 7299 8243 Size: 469 89 265 18 78 347 253 944 Cuts at: 8243 8871 9246 9755 9907 10047 10306 10479 11028 Size: 628 375 509 152 140 259 173 549 Cuts at: 11028 11533 11676 12468 12562 12983 13788 13873 14944 Size: 505 143 792 94 421 805 85 1071 Cuts at: 14944 2037 Size: 2820 Fragments arranged by size: 2820 2360 1071 944 805 792 628 549 509 505 469 421 375 347 344 340 336 265 259 255 253 173 152 143 140 94 89 85 78 51 32 25 18 BsmI GAATG_Cn' Cuts at: 3044 4000 13238 13331 3044 Size: 956 9238 93 5440 Fragments arranged by size: 9238 5440 956 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2120 2830 5049 6966 12566 2120 Size: 710 2219 1917 5600 5281 Fragments arranged by size: 5600 5281 2219 1917 710 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2055 2292 2343 2368 3367 3766 Size: 399 247 18 237 51 25 999 399 Cuts at: 3766 3818 3895 3904 4481 4512 4654 4675 4728 Size: 52 77 9 577 31 142 21 53 Cuts at: 4728 5064 5096 5290 5436 5544 5780 5812 6027 Size: 336 32 194 146 108 236 32 215 Cuts at: 6027 6249 6328 6338 6603 6621 6670 6699 6777 Size: 222 79 10 265 18 49 29 78 Cuts at: 6777 6862 7046 7147 7299 7410 7667 7816 7831 Size: 85 184 101 152 111 257 149 15 Cuts at: 7831 7957 8083 8122 8243 8512 8871 9246 9382 Size: 126 126 39 121 269 359 375 136 Cuts at: 9382 9478 9494 9627 9755 9843 9907 9945 9964 Size: 96 16 133 128 88 64 38 19 Cuts at: 9964 10047 10306 10419 10441 10479 10487 10647 10868 Size: 83 259 113 22 38 8 160 221 Cuts at: 10868 10896 11028 11301 11533 11676 11995 12235 12354 Size: 28 132 273 232 143 319 240 119 Cuts at: 12354 12468 12562 12787 12910 12941 12983 13046 13788 Size: 114 94 225 123 31 42 63 742 Cuts at: 13788 13873 14944 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 999 742 577 399 399 375 359 336 319 273 269 265 259 257 247 240 237 236 232 225 222 221 215 194 184 160 152 149 146 143 142 136 133 132 128 126 126 123 121 119 114 113 111 108 101 96 94 88 85 85 83 79 78 77 64 63 53 52 51 49 42 39 38 38 32 32 31 31 29 28 25 22 21 19 18 18 16 15 10 9 8 BspEI T'CCGG_A Cuts at: 2235 6029 6501 9369 15390 2235 Size: 3794 472 2868 6021 2572 Fragments arranged by size: 6021 3794 2868 2572 472 BspHI T'CATG_A Cuts at: 2814 3166 8616 13616 15346 2814 Size: 352 5450 5000 1730 3195 Fragments arranged by size: 5450 5000 3195 1730 352 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3334 3577 3741 5114 5451 6115 6363 6718 Size: 2490 243 164 1373 337 664 248 355 Cuts at: 6718 7236 7894 7908 9960 10803 10962 12129 12648 Size: 518 658 14 2052 843 159 1167 519 Cuts at: 12648 13082 844 Size: 434 3489 Fragments arranged by size: 3489 2490 2052 1373 1167 843 664 658 519 518 434 355 337 248 243 164 159 14 BsrBI CCG'CTC Cuts at: 1394 1823 4294 7672 7825 8335 9235 9497 11056 Size: 429 2471 3378 153 510 900 262 1559 Cuts at: 11056 11173 11524 13614 1394 Size: 117 351 2090 3507 Fragments arranged by size: 3507 3378 2471 2090 1559 900 510 429 351 262 153 117 BsrDI GCAATG_nn' Cuts at: 65 1248 2754 8739 9441 14222 14396 65 Size: 1183 1506 5985 702 4781 174 1396 Fragments arranged by size: 5985 4781 1506 1396 1183 702 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3667 7374 8370 8527 14371 14558 1700 Size: 110 1857 3707 996 157 5844 187 2869 Fragments arranged by size: 5844 3707 2869 1857 996 187 157 110 BsrGI T'GTAC_A Cuts at: 96 2244 96 Size: 2148 13579 BssHII G'CGCG_C Cuts at: 1925 4387 6035 1925 Size: 2462 1648 11617 Fragments arranged by size: 11617 2462 1648 BssSI C'ACGA_G Cuts at: 4097 7576 7658 8276 9221 9487 10838 10907 13787 Size: 3479 82 618 945 266 1351 69 2880 Cuts at: 13787 14799 4097 Size: 1012 5025 Fragments arranged by size: 5025 3479 2880 1351 1012 945 618 266 82 69 BstAPI GCAn_nnn'nTGC Cuts at: 794 3227 3607 3733 3919 4753 5251 5614 6355 Size: 2433 380 126 186 834 498 363 741 Cuts at: 6355 6739 7303 7447 7687 8036 8701 8875 9208 Size: 384 564 144 240 349 665 174 333 Cuts at: 9208 9448 9610 9862 10222 11305 12640 12643 794 Size: 240 162 252 360 1083 1335 3 3878 Fragments arranged by size: 3878 2433 1335 1083 834 741 665 564 498 384 380 363 360 349 333 252 240 240 186 174 162 144 126 3 BstBI TT'CG_AA Cuts at: 14531 14531 Size: 15727 BstEII G'GTnAC_C Cuts at: 1184 8846 1184 Size: 7662 8065 BstXI CCAn_nnnn'nTGG Cuts at: 3853 4512 6257 7594 8017 8233 10766 3853 Size: 659 1745 1337 423 216 2533 8814 Fragments arranged by size: 8814 2533 1745 1337 659 423 216 BstYI r'GATC_y Cuts at: 1972 2232 2481 2892 4012 4093 4147 5454 7444 Size: 260 249 411 1120 81 54 1307 1990 Cuts at: 7444 7537 8193 8496 8814 9187 11039 12858 13057 Size: 93 656 303 318 373 1852 1819 199 Cuts at: 13057 13424 13809 13826 15278 15364 15376 15722 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1990 1977 1852 1819 1452 1307 1120 656 411 385 373 367 346 318 303 260 249 199 93 86 81 54 17 12 Bsu36I CC'TnA_GG Cuts at: 2378 4496 6278 6769 6989 7193 7211 7731 8723 Size: 2118 1782 491 220 204 18 520 992 Cuts at: 8723 8741 8991 9326 10637 11498 2378 Size: 18 250 335 1311 861 6607 Fragments arranged by size: 6607 2118 1782 1311 992 861 520 491 335 250 220 204 18 18 BtgI C'CryG_G Cuts at: 513 1065 2004 2109 3757 4689 5505 6444 6451 Size: 552 939 105 1648 932 816 939 7 Cuts at: 6451 6628 7047 7422 9052 9319 9834 9915 9922 Size: 177 419 375 1630 267 515 81 7 Cuts at: 9922 10944 12609 12688 12811 513 Size: 1022 1665 79 123 3429 Fragments arranged by size: 3429 1665 1648 1630 1022 939 939 932 816 552 515 419 375 267 177 123 105 81 79 7 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2640 10016 487 Size: 683 56 1414 7376 6198 Fragments arranged by size: 7376 6198 1414 683 56 BtsI GCAGTG_nn' Cuts at: 2705 4824 5240 5615 5664 5993 6809 7145 7788 Size: 2119 416 375 49 329 816 336 643 Cuts at: 7788 7939 8869 8966 10515 10538 10576 12918 13243 Size: 151 930 97 1549 23 38 2342 325 Cuts at: 13243 14037 14057 2705 Size: 794 20 4375 Fragments arranged by size: 4375 2342 2119 1549 930 816 794 643 416 375 336 329 325 151 97 49 38 23 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 15727 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 15692 DraI TTT'AAA Cuts at: 13031 13031 Size: 15727 DraIII CAC_nnn'GTG Cuts at: 2190 5000 5002 6796 7342 10912 12358 2190 Size: 2810 2 1794 546 3570 1446 5559 Fragments arranged by size: 5559 3570 2810 1794 1446 546 2 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7459 14734 809 Size: 507 6143 7275 1802 Fragments arranged by size: 7275 6143 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2215 3381 3669 Size: 54 1425 50 275 18 385 1166 288 Cuts at: 3669 4338 4398 5644 6160 6459 6471 6531 6941 Size: 669 60 1246 516 299 12 60 410 Cuts at: 6941 7161 7365 8179 8457 8916 10344 10749 10768 Size: 220 204 814 278 459 1428 405 19 Cuts at: 10768 10914 11379 11871 13174 14063 15692 8 Size: 146 465 492 1303 889 1629 43 Fragments arranged by size: 1629 1428 1425 1303 1246 1166 889 814 669 516 492 465 459 410 405 385 299 288 278 275 220 204 146 60 60 54 50 43 19 18 12 EagI C'GGCC_G Cuts at: 1537 1812 4338 4398 6941 8179 8916 13174 1537 Size: 275 2526 60 2543 1238 737 4258 4090 Fragments arranged by size: 4258 4090 2543 2526 1238 737 275 60 EarI CTCTTCn'nnn_ Cuts at: 1353 3119 4964 5991 6477 8064 8168 8244 9528 Size: 1766 1845 1027 486 1587 104 76 1284 Cuts at: 9528 10010 10593 11430 12069 12417 12876 13657 1353 Size: 482 583 837 639 348 459 781 3423 Fragments arranged by size: 3423 1845 1766 1587 1284 1027 837 781 639 583 486 482 459 348 104 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4283 6769 7400 7988 8432 8782 Size: 1064 138 2694 2486 631 588 444 350 Cuts at: 8782 10202 10399 11300 14314 14688 14834 387 Size: 1420 197 901 3014 374 146 1280 Fragments arranged by size: 3014 2694 2486 1420 1280 1064 901 631 588 444 374 350 197 146 138 EcoICRI GAG'CTC Cuts at: 2035 2290 2366 4726 5094 6247 6336 6601 6697 Size: 255 76 2360 368 1153 89 265 96 Cuts at: 6697 7044 8241 9753 10045 10477 11026 11674 12466 Size: 347 1197 1512 292 432 549 648 792 Cuts at: 12466 2035 Size: 5296 Fragments arranged by size: 5296 2360 1512 1197 1153 792 648 549 432 368 347 292 265 255 96 89 76 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2306 2369 2641 2885 2891 3011 3233 Size: 393 674 63 272 244 6 120 222 Cuts at: 3233 3440 3545 3671 3758 3929 3989 4178 4328 Size: 207 105 126 87 171 60 189 150 Cuts at: 4328 4394 4436 4467 4534 4592 4712 4760 4811 Size: 66 42 31 67 58 120 48 51 Cuts at: 4811 4979 4988 5051 5093 5168 5276 5468 5603 Size: 168 9 63 42 75 108 192 135 Cuts at: 5603 5648 5885 5930 5936 6233 6329 6346 6416 Size: 45 237 45 6 297 96 17 70 Cuts at: 6416 6530 6683 6857 6938 7060 7202 7637 7667 Size: 114 153 174 81 122 142 435 30 Cuts at: 7667 7712 7841 7964 8000 8207 8309 8360 8540 Size: 45 129 123 36 207 102 51 180 Cuts at: 8540 8579 8613 8777 8798 8885 9212 9251 9413 Size: 39 34 164 21 87 327 39 162 Cuts at: 9413 9616 9752 9887 9899 9929 9991 10058 10310 Size: 203 136 135 12 30 62 67 252 Cuts at: 10310 10337 10421 10433 10520 10604 10684 10691 10838 Size: 27 84 12 87 84 80 7 147 Cuts at: 10838 10955 11051 11118 11147 11219 11354 11435 11540 Size: 117 96 67 29 72 135 81 105 Cuts at: 11540 11660 11795 12239 12311 12356 12509 12652 13790 Size: 120 135 444 72 45 153 143 1138 Cuts at: 13790 14387 15174 15442 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1138 787 674 597 444 435 393 327 297 272 268 252 244 237 222 207 207 203 192 189 180 174 171 168 164 162 153 153 150 147 143 142 136 135 135 135 135 129 126 123 122 120 120 120 117 114 108 105 105 102 96 96 87 87 87 84 84 81 81 80 75 72 72 70 67 67 67 66 63 63 62 60 58 51 51 48 45 45 45 45 42 42 39 39 36 34 31 30 30 29 27 21 17 12 12 9 7 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 2377 2858 3437 3485 3620 3737 5223 5447 5465 Size: 481 579 48 135 117 1486 224 18 Cuts at: 5465 5714 6122 7214 7283 7556 8726 8804 8855 Size: 249 408 1092 69 273 1170 78 51 Cuts at: 8855 8944 9164 9932 10564 11477 11708 11873 12125 Size: 89 220 768 632 913 231 165 252 Cuts at: 12125 2377 Size: 5979 Fragments arranged by size: 5979 1486 1170 1092 913 768 632 579 481 408 273 252 249 231 224 220 165 135 117 89 78 69 51 48 18 EcoO109I rG'GnC_Cy Cuts at: 2086 2305 3106 3153 3172 3241 3457 3891 3901 Size: 219 801 47 19 69 216 434 10 Cuts at: 3901 4051 4131 4508 4509 4651 4671 4846 4963 Size: 150 80 377 1 142 20 175 117 Cuts at: 4963 5077 5557 5731 5786 6115 6153 6324 6325 Size: 114 480 174 55 329 38 171 1 Cuts at: 6325 6352 6541 7188 7225 7288 7603 7727 7812 Size: 27 189 647 37 63 315 124 85 Cuts at: 7812 7897 8542 8698 8719 8860 8986 9090 9169 Size: 85 645 156 21 141 126 104 79 Cuts at: 9169 9378 9693 9727 9820 9874 10020 10369 10456 Size: 209 315 34 93 54 146 349 87 Cuts at: 10456 11110 11410 11866 12118 12232 12265 12534 12580 Size: 654 300 456 252 114 33 269 46 Cuts at: 12580 12693 2086 Size: 113 5120 Fragments arranged by size: 5120 801 654 647 645 480 456 434 377 349 329 315 315 300 269 252 219 216 209 189 175 174 171 156 150 146 142 141 126 124 117 114 114 113 104 93 87 85 85 80 79 69 63 55 54 47 46 38 37 34 33 27 21 20 19 10 1 1 EcoRI G'AATT_C Cuts at: 13036 13414 13036 Size: 378 15349 EcoRV GAT'ATC Cuts at: 4895 4895 Size: 15727 FspI TGC'GCA Cuts at: 3941 5381 6018 14233 3941 Size: 1440 637 8215 5435 Fragments arranged by size: 8215 5435 1440 637 HaeII r_GCGC'y Cuts at: 1678 2809 3295 3451 4035 4125 4363 5296 8356 Size: 1131 486 156 584 90 238 933 3060 Cuts at: 8356 8395 9401 9462 10004 11542 11773 11971 14874 Size: 39 1006 61 542 1538 231 198 2903 Cuts at: 14874 1678 Size: 2531 Fragments arranged by size: 3060 2903 2531 1538 1131 1006 933 584 542 486 238 231 198 156 90 61 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2669 2701 2792 2824 4101 Size: 32 135 32 1097 32 91 32 1277 Cuts at: 4101 4133 4283 4301 4315 4333 5393 5425 6344 Size: 32 150 18 14 18 1060 32 919 Cuts at: 6344 6376 7856 7888 8877 8909 9889 9921 11024 Size: 32 1480 32 989 32 980 32 1103 Cuts at: 11024 11056 11999 12031 12996 13028 14368 14400 14442 Size: 32 943 32 965 32 1340 32 42 Cuts at: 14442 14474 1373 Size: 32 2626 Fragments arranged by size: 2626 1480 1340 1277 1103 1097 1060 989 980 965 943 919 150 135 91 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 13071 13317 669 Size: 988 281 11133 246 3079 Fragments arranged by size: 11133 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 6210 12275 748 Size: 5462 6065 4200 Fragments arranged by size: 6065 5462 4200 HpaI GTT'AAC Cuts at: 13317 13317 Size: 15727 KasI G'GCGC_C Cuts at: 4031 4359 5292 8352 11769 4031 Size: 328 933 3060 3417 7989 Fragments arranged by size: 7989 3417 3060 933 328 KpnI G_GTAC'C Cuts at: 9073 12603 13052 9073 Size: 3530 449 11748 Fragments arranged by size: 11748 3530 449 MfeI C'AATT_G Cuts at: 13326 13326 Size: 15727 MluI A'CGCG_T Cuts at: 15603 15603 Size: 15727 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2655 3889 4659 4942 7443 8908 9663 11574 Size: 1350 1234 770 283 2501 1465 755 1911 Cuts at: 11574 14841 15025 1305 Size: 3267 184 2007 Fragments arranged by size: 3267 2501 2007 1911 1465 1350 1234 770 755 283 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3383 6162 6533 8459 10346 Size: 54 1425 343 1551 2779 371 1926 1887 Cuts at: 10346 10770 11873 10 Size: 424 1103 3864 Fragments arranged by size: 3864 2779 1926 1887 1551 1425 1103 424 371 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2165 2591 3821 7856 9936 10145 13685 Size: 648 999 426 1230 4035 2080 209 3540 Cuts at: 13685 14044 14203 518 Size: 359 159 2042 Fragments arranged by size: 4035 3540 2080 2042 1230 999 648 426 359 209 159 MspA1I CmG'CkG Cuts at: 1606 2569 2764 2944 2989 3265 3271 3504 3649 Size: 963 195 180 45 276 6 233 145 Cuts at: 3649 3697 3925 3985 4189 4571 4957 4990 5050 Size: 48 228 60 204 382 386 33 60 Cuts at: 5050 5071 5122 5377 5419 5485 5846 5869 6077 Size: 21 51 255 42 66 361 23 208 Cuts at: 6077 6286 6307 6394 6550 6946 7138 7360 7627 Size: 209 21 87 156 396 192 222 267 Cuts at: 7627 7678 7819 7942 8017 8095 8518 8689 9016 Size: 51 141 123 75 78 423 171 327 Cuts at: 9016 9620 9718 9742 9924 10054 10228 10754 10933 Size: 604 98 24 182 130 174 526 179 Cuts at: 10933 11242 11317 11332 11669 12142 12565 12684 12690 Size: 309 75 15 337 473 423 119 6 Cuts at: 12690 12743 13143 13820 14968 15213 1606 Size: 53 400 677 1148 245 2120 Fragments arranged by size: 2120 1148 963 677 604 526 473 423 423 400 396 386 382 361 337 327 309 276 267 255 245 233 228 222 209 208 204 195 192 182 180 179 174 171 156 145 141 130 123 119 98 87 78 75 75 66 60 60 53 51 51 48 45 42 33 24 23 21 21 15 6 6 NaeI GCC'GGC Cuts at: 3669 7376 3669 Size: 3707 12020 NarI GG'CG_CC Cuts at: 4032 4360 5293 8353 11770 4032 Size: 328 933 3060 3417 7989 Fragments arranged by size: 7989 3417 3060 933 328 NcoI C'CATG_G Cuts at: 513 1065 2004 2109 4689 5505 6444 6451 6628 Size: 552 939 105 2580 816 939 7 177 Cuts at: 6628 7047 7422 9052 9834 10944 12609 513 Size: 419 375 1630 782 1110 1665 3631 Fragments arranged by size: 3631 2580 1665 1630 1110 939 939 816 782 552 419 375 177 105 7 NdeI CA'TA_TG Cuts at: 387 387 Size: 15727 NgoMIV G'CCGG_C Cuts at: 3667 7374 3667 Size: 3707 12020 NheI G'CTAG_C Cuts at: 1052 11233 11983 1052 Size: 10181 750 4796 Fragments arranged by size: 10181 4796 750 NotI GC'GGCC_GC Cuts at: 4338 6941 8179 13174 4338 Size: 2603 1238 4995 6891 Fragments arranged by size: 6891 4995 2603 1238 NspI r_CATG'y Cuts at: 1132 1229 2164 3418 3601 6625 6849 8444 10207 Size: 97 935 1254 183 3024 224 1595 1763 Cuts at: 10207 13085 14630 1132 Size: 2878 1545 2229 Fragments arranged by size: 3024 2878 2229 1763 1595 1545 1254 935 224 183 97 PasI CC'CwG_GG Cuts at: 1348 3811 4003 4505 4541 5782 9964 12910 1348 Size: 2463 192 502 36 1241 4182 2946 4165 Fragments arranged by size: 4182 4165 2946 2463 1241 502 192 36 PciI A'CATG_T Cuts at: 14626 14626 Size: 15727 PflMI CCAn_nnn'nTGG Cuts at: 1108 3409 3460 3649 5176 5506 6394 6457 6814 Size: 2301 51 189 1527 330 888 63 357 Cuts at: 6814 7038 7363 7645 8755 8863 9172 9655 10036 Size: 224 325 282 1110 108 309 483 381 Cuts at: 10036 10084 10594 10816 10912 11068 11932 12675 1108 Size: 48 510 222 96 156 864 743 4160 Fragments arranged by size: 4160 2301 1527 1110 888 864 743 510 483 381 357 330 325 309 282 224 222 189 156 108 96 63 51 48 PfoI T'CCnGG_A Cuts at: 2346 2907 3315 4483 5952 6477 7128 7563 8064 Size: 561 408 1168 1469 525 651 435 501 Cuts at: 8064 8775 8817 9566 10278 11028 11067 11196 12723 Size: 711 42 749 712 750 39 129 1527 Cuts at: 12723 2346 Size: 5350 Fragments arranged by size: 5350 1527 1469 1168 750 749 712 711 651 561 525 501 435 408 129 42 39 PmeI GTTT'AAAC Cuts at: 13031 13031 Size: 15727 PmlI CAC'GTG Cuts at: 4366 6109 9106 4366 Size: 1743 2997 10987 Fragments arranged by size: 10987 2997 1743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 12450 12482 13796 13828 15309 15341 12450 Size: 32 1314 32 1481 32 12836 Fragments arranged by size: 12836 1481 1314 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2086 5557 6352 7188 7225 7288 7603 7897 8542 Size: 3471 795 836 37 63 315 294 645 Cuts at: 8542 8719 8860 8986 9820 9874 10020 11410 12118 Size: 177 141 126 834 54 146 1390 708 Cuts at: 12118 12580 12693 2086 Size: 462 113 5120 Fragments arranged by size: 5120 3471 1390 836 834 795 708 645 462 315 294 177 146 141 126 113 63 54 37 PshAI GACnn'nnGTC Cuts at: 1613 2822 1613 Size: 1209 14518 PsiI TTA'TAA Cuts at: 13297 13297 Size: 15727 PspOMI G'GGCC_C Cuts at: 3891 3900 4508 4650 4671 5286 6324 6858 7812 Size: 9 608 142 21 615 1038 534 954 Cuts at: 7812 8508 9378 12231 3891 Size: 696 870 2853 7387 Fragments arranged by size: 7387 2853 1038 954 870 696 615 608 534 142 21 9 PspXI vC'TCGA_Gb Cuts at: 8667 8667 Size: 15727 PstI C_TGCA'G Cuts at: 830 2050 2928 3249 3264 3270 3624 3663 3723 Size: 1220 878 321 15 6 354 39 60 Cuts at: 3723 3837 3924 4041 4230 4251 4712 5025 5085 Size: 114 87 117 189 21 461 313 60 Cuts at: 5085 5121 5247 5481 5637 5748 5874 6054 6123 Size: 36 126 234 156 111 126 180 69 Cuts at: 6123 6291 6378 6522 6549 6555 6873 7152 7233 Size: 168 87 144 27 6 318 279 81 Cuts at: 7233 7452 7683 7702 7791 7905 8044 8088 8142 Size: 219 231 19 89 114 139 44 54 Cuts at: 8142 8391 8697 8790 8880 8973 9444 9723 10059 Size: 249 306 93 90 93 471 279 336 Cuts at: 10059 10071 10227 10251 10653 10800 10992 11181 11247 Size: 12 156 24 402 147 192 189 66 Cuts at: 11247 11310 11322 11635 11757 11857 11904 12192 12213 Size: 63 12 313 122 100 47 288 21 Cuts at: 12213 12273 12477 12925 13079 830 Size: 60 204 448 154 3478 Fragments arranged by size: 3478 1220 878 471 461 448 402 354 336 321 318 313 313 306 288 279 279 249 234 231 219 204 192 189 189 180 168 156 156 154 147 144 139 126 126 122 117 114 114 111 100 93 93 90 89 87 87 81 69 66 63 60 60 60 54 47 44 39 36 27 24 21 21 19 15 12 12 6 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 15727 PvuII CAG'CTG Cuts at: 2764 2944 2989 3265 3504 3649 3697 4189 4571 Size: 180 45 276 239 145 48 492 382 Cuts at: 4571 4957 4990 5050 5071 5122 5377 5485 5846 Size: 386 33 60 21 51 255 108 361 Cuts at: 5846 5869 6077 6286 6307 6394 6550 7138 7627 Size: 23 208 209 21 87 156 588 489 Cuts at: 7627 7678 7819 8017 8518 9718 9742 10054 10933 Size: 51 141 198 501 1200 24 312 879 Cuts at: 10933 11242 11317 11332 11669 12142 12565 12684 2764 Size: 309 75 15 337 473 423 119 5807 Fragments arranged by size: 5807 1200 879 588 501 492 489 473 423 386 382 361 337 312 309 276 255 239 209 208 198 180 156 145 141 119 108 87 75 60 51 51 48 45 33 24 23 21 21 15 SacI G_AGCT'C Cuts at: 2037 2292 2368 4728 5096 6249 6338 6603 6699 Size: 255 76 2360 368 1153 89 265 96 Cuts at: 6699 7046 8243 9755 10047 10479 11028 11676 12468 Size: 347 1197 1512 292 432 549 648 792 Cuts at: 12468 2037 Size: 5296 Fragments arranged by size: 5296 2360 1512 1197 1153 792 648 549 432 368 347 292 265 255 96 89 76 SacII CC_GC'GG Cuts at: 9925 12691 9925 Size: 2766 12961 SalI G'TCGA_C Cuts at: 1936 13069 1936 Size: 11133 4594 SanDI GG'GwC_CC Cuts at: 2086 7188 8986 9820 10020 12580 2086 Size: 5102 1798 834 200 2560 5233 Fragments arranged by size: 5233 5102 2560 1798 834 200 SapI GCTCTTCn'nnn_ Cuts at: 3119 8064 8168 9528 10010 10593 12069 12876 3119 Size: 4945 104 1360 482 583 1476 807 5970 Fragments arranged by size: 5970 4945 1476 1360 807 583 482 104 SbfI CC_TGCA'GG Cuts at: 7233 7905 8088 10071 10653 10800 11310 11904 12192 Size: 672 183 1983 582 147 510 594 288 Cuts at: 12192 13079 7233 Size: 887 9881 Fragments arranged by size: 9881 1983 887 672 594 582 510 288 183 147 ScaI AGT'ACT Cuts at: 4253 6896 13975 15687 4253 Size: 2643 7079 1712 4293 Fragments arranged by size: 7079 4293 2643 1712 SexAI A'CCwGG_T Cuts at: 8100 10110 8100 Size: 2010 13717 SfcI C'TryA_G Cuts at: 826 1045 2046 2147 2924 3083 3122 3245 3260 Size: 219 1001 101 777 159 39 123 15 Cuts at: 3260 3266 3569 3620 3659 3719 3833 3920 4037 Size: 6 303 51 39 60 114 87 117 Cuts at: 4037 4226 4247 4708 5021 5081 5117 5243 5477 Size: 189 21 461 313 60 36 126 234 Cuts at: 5477 5633 5744 5870 6050 6119 6287 6329 6374 Size: 156 111 126 180 69 168 42 45 Cuts at: 6374 6518 6545 6551 6869 7016 7148 7229 7448 Size: 144 27 6 318 147 132 81 219 Cuts at: 7448 7679 7698 7787 7901 8040 8084 8138 8387 Size: 231 19 89 114 139 44 54 249 Cuts at: 8387 8693 8786 8876 8969 9440 9713 9719 9737 Size: 306 93 90 93 471 273 6 18 Cuts at: 9737 10055 10067 10223 10247 10649 10796 10988 11177 Size: 318 12 156 24 402 147 192 189 Cuts at: 11177 11243 11306 11318 11631 11753 11816 11853 11900 Size: 66 63 12 313 122 63 37 47 Cuts at: 11900 12188 12209 12269 12473 12921 13075 14210 14891 Size: 288 21 60 204 448 154 1135 681 Cuts at: 14891 15082 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1001 777 681 471 461 448 402 318 318 313 313 306 303 288 273 249 234 231 219 219 204 192 191 189 189 180 168 159 156 156 154 147 147 144 139 132 126 126 123 122 117 114 114 111 101 93 93 90 89 87 81 69 66 63 63 60 60 60 54 51 47 45 44 42 39 39 37 36 27 24 21 21 19 18 15 12 12 6 6 6 SfiI GGCCn_nnn'nGGCC Cuts at: 3104 3899 5285 10367 10673 12311 3104 Size: 795 1386 5082 306 1638 6520 Fragments arranged by size: 6520 5082 1638 1386 795 306 SfoI GGC'GCC Cuts at: 4033 4361 5294 8354 11771 4033 Size: 328 933 3060 3417 7989 Fragments arranged by size: 7989 3417 3060 933 328 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 15727 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 15727 SmaI CCC'GGG Cuts at: 1872 2030 4153 4202 4445 7988 8360 10853 10922 Size: 158 2123 49 243 3543 372 2493 69 Cuts at: 10922 12496 13054 1872 Size: 1574 558 4545 Fragments arranged by size: 4545 3543 2493 2123 1574 558 372 243 158 69 49 SmlI C'TyrA_G Cuts at: 820 1017 1943 3329 3744 4624 6059 8667 8975 Size: 197 926 1386 415 880 1435 2608 308 Cuts at: 8975 12835 13831 14732 14994 820 Size: 3860 996 901 262 1553 Fragments arranged by size: 3860 2608 1553 1435 1386 996 926 901 880 415 308 262 197 SnaBI TAC'GTA Cuts at: 493 13518 493 Size: 13025 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 15727 SphI G_CATG'C Cuts at: 1132 3418 8444 10207 13085 1132 Size: 2286 5026 1763 2878 3774 Fragments arranged by size: 5026 3774 2878 2286 1763 SrfI GCCC'GGGC Cuts at: 4445 4445 Size: 15727 SspI AAT'ATT Cuts at: 5 52 13651 5 Size: 47 13599 2081 Fragments arranged by size: 13599 2081 47 StuI AGG'CCT Cuts at: 1517 5690 6192 6557 8582 8810 10061 10361 10559 Size: 4173 502 365 2025 228 1251 300 198 Cuts at: 10559 11888 12305 1517 Size: 1329 417 4939 Fragments arranged by size: 4939 4173 2025 1329 1251 502 417 365 300 228 198 StyI C'CwwG_G Cuts at: 513 1065 2004 2109 2385 2496 2541 3840 4689 Size: 552 939 105 276 111 45 1299 849 Cuts at: 4689 5505 5514 5710 6444 6451 6628 7047 7422 Size: 816 9 196 734 7 177 419 375 Cuts at: 7422 8055 8232 9052 9093 9825 9834 10119 10944 Size: 633 177 820 41 732 9 285 825 Cuts at: 10944 11226 11373 11484 11874 12061 12609 12987 13166 Size: 282 147 111 390 187 548 378 179 Cuts at: 13166 513 Size: 3074 Fragments arranged by size: 3074 1299 939 849 825 820 816 734 732 633 552 548 419 390 378 375 285 282 276 196 187 179 177 177 147 111 111 105 45 41 9 9 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3077 5856 6916 7571 8027 8065 8483 Size: 1255 1497 2779 1060 655 456 38 418 Cuts at: 8483 8921 10275 10717 11548 13754 13771 13924 14109 Size: 438 1354 442 831 2206 17 153 185 Cuts at: 14109 14554 325 Size: 445 1498 Fragments arranged by size: 2779 2206 1498 1497 1354 1255 1060 831 655 456 445 442 438 418 185 153 38 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2244 4251 5263 Size: 274 80 33 51 1443 267 2007 1012 Cuts at: 5263 6894 13973 15685 96 Size: 1631 7079 1712 138 Fragments arranged by size: 7079 2007 1712 1631 1443 1012 274 267 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2831 2863 3753 3785 3972 4004 6142 6174 7982 Size: 32 890 32 187 32 2138 32 1808 Cuts at: 7982 8014 8941 8973 9095 9127 12815 12847 15687 Size: 32 927 32 122 32 3688 32 2840 Cuts at: 15687 15719 2831 Size: 32 2839 Fragments arranged by size: 3688 2840 2839 2138 1808 927 890 187 122 32 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 9128 1316 Size: 7812 7915 XbaI T'CTAG_A Cuts at: 13063 13063 Size: 15727 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4538 5512 7079 9656 10460 11143 4538 Size: 974 1567 2577 804 683 9122 Fragments arranged by size: 9122 2577 1567 974 804 683 XhoI C'TCGA_G Cuts at: 1943 8667 1943 Size: 6724 9003 XmaI C'CCGG_G Cuts at: 1870 2028 4151 4200 4443 7986 8358 10851 10920 Size: 158 2123 49 243 3543 372 2493 69 Cuts at: 10920 12494 13052 1870 Size: 1574 558 4545 Fragments arranged by size: 4545 3543 2493 2123 1574 558 372 243 158 69 49 XmnI GAAnn'nnTTC Cuts at: 2180 8073 10697 13856 2180 Size: 5893 2624 3159 4051 Fragments arranged by size: 5893 4051 3159 2624 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 13535 276 Size: 53 83 186 936 12001 2468 Fragments arranged by size: 12001 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BfrBI BsiWI BstZ17I FalI FseI FspAI NruI NsiI PacI PsrI RsrII SwaI