Restriction map for
pFN21AE2241
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
Acc65I AclI AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BalI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfaI BglII BlnI BpuAI
BsaI BseRI BsiEI BsiWI BsmBI
BspDI BsrDI BsrFI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI HindIII
HpaI KasI KpnI KspI MaeI
MfeI MluI MluNI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 211 GACGTC GACGT^C
AccI 199 GTCTAC GT^[AC][GT]AC
AccIII 1391 TCCGGA T^CCGGA
AcsI 980 GAATTC [GA]^AATT[CT]
AflII 121 CTTAAG C^TTAAG
AhdI 325 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 993 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 268 GGGCCC GGGCC^C
ApoI 980 GAATTC [GA]^AATT[CT]
AspEI 325 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 628 GAGCTC G[AT]GC[AT]^C
AvaI 340 CTCGAG C^[CT]CG[GA]G
AviII 131 TGCGCA TGC^GCA
BamHI 689 GGATCC G^GATCC
BanI 93 GGTGCC G^G[CT][GA]CC
BclI 758 TGATCA T^GATCA
BfrI 121 CTTAAG C^TTAAG
BglI 1456 GCCATTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 274 TACGTG [CT]AC^GT[GA]
BsaBI 174 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1391 TCCGGA [AT]^CCGG[AT]
BseAI 1391 TCCGGA T^CCGGA
BsgI 1081 CTGCAC GTGCAG(16/14)
BsiHKAI 628 GAGCTC G[AT]GC[AT]^C
BsmAI 1273 GAGAC GTCTC(1/5)
BsoBI 340 CTCGAG C^[CT]CG[GA]G
BspEI 1391 TCCGGA T^CCGGA
BspLU11I 161 ACATGT A^CATGT
BsrBI 1254 GAGCGG CCGCTC(-3/-3)
BssHII 19 GCGCGC G^CGCGC
BssSI 697 CTCGTG CACGAG(-5/-1)
BstXI 410 CCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 694 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 784 TGGCCG [CT]^GGCC[GA]
Eam1105I 325 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 980 GAATTC G^AATTC
FbaI 758 TGATCA T^GATCA
FspI 131 TGCGCA TGC^GCA
HincII 727 GTTGAC GT[CT]^[GA]AC
HindII 727 GTTGAC GT[CT]^[GA]AC
MamI 174 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1391 TCCGGA T^CCGGA
MseI 122 TTAA T^TAA
NcoI 1262 CCATGG C^CATGG
PaeR7I 340 CTCGAG C^TCGAG
PflMI 693 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 628 GAGCTC GAGCT^C
SapI 651 GCTCTTC GCTCTTC(1/4)
SbfI 998 CCTGCAGG CCTGCA^GG
ScaI 578 AGTACT AGT^ACT
SfiI 1455 GGCCATTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 998 CCTGCAGG CCTGCA^GG
Tru9I 122 TTAA T^TAA
Tsp509I 981 AATT ^AATT
Van91I 693 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 340 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 43 GGCGTC G[GA]^CG[CT]C
211 GACGTC
AflIII 161 ACATGT A^C[GA][CT]GT
275 ACGTGT
BpmI 814 CTGGAG CTGGAG(16/14)
970 CTGGAG
BsaHI 43 GGCGTC G[GA]^CG[CT]C
211 GACGTC
BsmFI 375 GTCCC GGGAC(10/14)
1372 GTCCC
BsmI 484 GCATTC GAATGC(1/-1)
738 GCATTC
Bsp1407I 278 TGTACA T^GTACA
857 TGTACA
BsrGI 278 TGTACA T^GTACA
857 TGTACA
DrdI 394 GACTACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
798 GACTCCTGAGTC
DsaI 378 CCACGG C^C[GA][CT]GG
1262 CCATGG
EarI 652 CTCTTC CTCTTC(1/4)
1277 CTCTTC
HgaI 44 GCGTC GACGC(5/10)
1358 GCGTC
Hin1I 43 GGCGTC G[GA]^CG[CT]C
211 GACGTC
Ksp632I 652 CTCTTC CTCTTC(1/4)
1277 CTCTTC
MaeIII 1072 GTGAC ^GT[ACGT]AC
1129 GTCAC
MslI 81 CATGATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
399 CATCGTCATG
NspI 161 ACATGT [GA]CATG^[CT]
590 GCATGT
PpuMI 116 AGGTCCT [GA]G^G[AT]CC[CT]
551 AGGACCC
PvuII 1165 CAGCTG CAG^CTG
1297 CAGCTG
SmlI 121 CTTAAG C^T[CT][GA]AG
340 CTCGAG
SspBI 278 TGTACA T^GTACA
857 TGTACA
StuI 833 AGGCCT AGG^CCT
1463 AGGCCT
TaqI 137 TCGA T^CGA
341 TCGA
Tsp45I 1072 GTGAC ^GT[GC]AC
1129 GTCAC
TthHB8I 137 TCGA T^CGA
341 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanII BbvI BcnI BlpI
BmyI Bpu1102I BsaJI BsiYI BslI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI TseI TspRI XhoII