(Circular) (Six-base) MAPSORT of: pFN21AE2241.seq Check: 595 from: 1 to: 6232 pFN21AE2241 6232 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2248 3011 2248 Size: 763 5469 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2220 4042 278 Size: 53 83 186 936 684 1822 2468 Fragments arranged by size: 2468 1822 936 684 186 83 53 AccI GT'mk_AC Cuts at: 1937 2205 3575 1937 Size: 268 1370 4594 Fragments arranged by size: 4594 1370 268 Acc65I G'GTAC_C Cuts at: 3553 3553 Size: 6232 AclI AA'CG_TT Cuts at: 1563 4359 4732 1563 Size: 2796 373 3063 Fragments arranged by size: 3063 2796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2261 2489 4295 5679 5947 1632 Size: 629 228 1806 1384 268 1917 Fragments arranged by size: 1917 1806 1384 629 268 228 AflII C'TTAA_G Cuts at: 820 1017 2126 820 Size: 197 1109 4926 Fragments arranged by size: 4926 1109 197 AflIII A'CryG_T Cuts at: 1203 2166 2280 5131 6108 1203 Size: 963 114 2851 977 1327 Fragments arranged by size: 2851 1327 977 963 114 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 2335 4961 2335 Size: 2626 3606 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6232 AlwNI CAG_nnn'CTG Cuts at: 3003 3975 5547 3003 Size: 972 1572 3688 Fragments arranged by size: 3688 1572 972 ApaI G_GGCC'C Cuts at: 2277 2277 Size: 6232 ApaLI G'TGCA_C Cuts at: 4289 5445 4289 Size: 1156 5076 ApoI r'AATT_y Cuts at: 1493 2985 3541 3767 3919 6028 1493 Size: 1492 556 226 152 2109 1697 Fragments arranged by size: 2109 1697 1492 556 226 152 AseI AT'TA_AT Cuts at: 160 1032 4786 160 Size: 872 3754 1606 Fragments arranged by size: 3754 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 2345 3557 1870 Size: 73 402 1212 4545 Fragments arranged by size: 4545 1212 402 73 BamHI G'GATC_C Cuts at: 2694 3562 3929 2694 Size: 868 367 4997 Fragments arranged by size: 4997 868 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2098 3553 5008 618 Size: 325 217 627 311 1455 1455 1842 Fragments arranged by size: 1842 1455 1455 627 325 311 217 BanII G_rGCy'C Cuts at: 1391 2277 2637 2658 3446 3509 3551 1391 Size: 886 360 21 788 63 42 4072 Fragments arranged by size: 4072 886 788 360 63 42 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5261 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4423 4457 1903 Size: 34 2486 34 3678 Fragments arranged by size: 3678 2486 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4124 5334 4124 Size: 1210 5022 BclI T'GATC_A Cuts at: 1548 2763 1548 Size: 1215 5017 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3467 4843 136 Size: 107 122 71 631 605 1795 1376 1525 Fragments arranged by size: 1795 1525 1376 631 605 122 107 71 BglII A'GATC_T Cuts at: 6227 6227 Size: 6232 BlpI GC'TnA_GC Cuts at: 2631 2723 3173 3649 2631 Size: 92 450 476 5214 Fragments arranged by size: 5214 476 450 92 Bme1580I G_kGCm'C Cuts at: 1790 2277 3019 4293 5449 1790 Size: 487 742 1274 1156 2573 Fragments arranged by size: 2573 1274 1156 742 487 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6232 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2142 3222 3331 Size: 416 53 83 399 24 725 1080 109 Cuts at: 3331 3963 4921 442 Size: 632 958 1753 Fragments arranged by size: 1753 1080 958 725 632 416 399 109 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6232 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3124 3124 Size: 6232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2840 2996 4892 1239 Size: 1601 156 1896 2579 Fragments arranged by size: 2579 1896 1601 156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4357 5222 5520 4357 Size: 865 298 5069 Fragments arranged by size: 5069 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4895 882 Size: 617 3396 2219 Fragments arranged by size: 3396 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2281 4023 493 Size: 711 1077 1742 2702 Fragments arranged by size: 2702 1742 1077 711 BsaBI GATnn'nnATC Cuts at: 2183 6226 2183 Size: 4043 2189 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 2217 4039 4421 Size: 53 83 186 936 516 168 1822 382 Cuts at: 4421 5056 275 Size: 635 1451 Fragments arranged by size: 1822 1451 936 635 516 382 186 168 83 53 BsaWI w'CCGG_w Cuts at: 3396 4665 5063 5337 5484 5895 3396 Size: 1269 398 274 147 411 3733 Fragments arranged by size: 3733 1269 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3418 3448 3481 3511 1178 Size: 30 165 30 2015 30 33 30 3899 Fragments arranged by size: 3899 2015 165 33 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6232 BseYI C'CCAG_C Cuts at: 2028 2044 2074 2228 3355 5435 5931 2028 Size: 16 30 154 1127 2080 496 2329 Fragments arranged by size: 2329 2080 1127 496 154 30 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3071 1163 Size: 573 1335 4324 Fragments arranged by size: 4324 1335 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3682 4443 5471 1540 Size: 275 186 1681 761 1028 2301 Fragments arranged by size: 2301 1681 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2637 4293 4378 5449 2637 Size: 1656 85 1071 3420 Fragments arranged by size: 3420 1656 1071 85 BsmI GAATG_Cn' Cuts at: 2489 2743 3743 3836 2489 Size: 254 1000 93 4885 Fragments arranged by size: 4885 1000 254 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 2637 2658 3019 3446 3509 3551 Size: 399 487 360 21 361 427 63 42 Cuts at: 3551 4293 4378 5449 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 487 427 399 361 360 85 63 42 21 BspEI T'CCGG_A Cuts at: 3396 5895 3396 Size: 2499 3733 BspHI T'CATG_A Cuts at: 2085 2220 2478 2517 2799 4121 5851 2085 Size: 135 258 39 282 1322 1730 2466 Fragments arranged by size: 2466 1730 1322 282 258 135 39 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2110 2248 2997 3011 3587 844 Size: 1266 138 749 14 576 3489 Fragments arranged by size: 3489 1266 749 576 138 14 BsrBI CCG'CTC Cuts at: 1394 1823 3261 4119 1394 Size: 429 1438 858 3507 Fragments arranged by size: 3507 1438 858 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4727 4901 65 Size: 1183 3479 174 1396 Fragments arranged by size: 3479 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4876 5063 1700 Size: 110 3066 187 2869 Fragments arranged by size: 3066 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2283 2862 96 Size: 2187 579 3466 Fragments arranged by size: 3466 2187 579 BssHII G'CGCG_C Cuts at: 1925 2024 1925 Size: 99 6133 BssSI C'ACGA_G Cuts at: 2702 4292 5304 2702 Size: 1590 1012 3630 Fragments arranged by size: 3630 1590 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6232 BstBI TT'CG_AA Cuts at: 5036 5036 Size: 6232 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6232 BstXI CCAn_nnnn'nTGG Cuts at: 2422 2422 Size: 6232 BstYI r'GATC_y Cuts at: 1972 2178 2235 2694 3562 3929 4314 4331 5783 Size: 206 57 459 868 367 385 17 1452 Cuts at: 5783 5869 5881 6227 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 868 459 385 367 346 206 86 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2383 3267 513 Size: 552 939 379 884 3478 Fragments arranged by size: 3478 939 884 552 379 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5493 Fragments arranged by size: 5493 683 56 BtsI GCAGTG_nn' Cuts at: 3748 4542 4562 3748 Size: 794 20 5418 Fragments arranged by size: 5418 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6197 DraI TTT'AAA Cuts at: 3536 3536 Size: 6232 DraIII CAC_nnn'GTG Cuts at: 2704 2704 Size: 6232 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2405 2809 5239 809 Size: 507 1089 404 2430 1802 Fragments arranged by size: 2430 1802 1089 507 404 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2789 3679 4568 Size: 54 1425 50 275 18 959 890 889 Cuts at: 4568 6197 8 Size: 1629 43 Fragments arranged by size: 1629 1425 959 890 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2663 3288 4162 1353 Size: 1310 625 874 3423 Fragments arranged by size: 3423 1310 874 625 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2005 4819 5193 5339 387 Size: 1064 138 416 2814 374 146 1280 Fragments arranged by size: 2814 1280 1064 416 374 146 138 EcoICRI GAG'CTC Cuts at: 2635 2635 Size: 6232 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2489 2840 2996 4295 4892 5679 Size: 393 629 228 351 156 1299 597 787 Cuts at: 5679 5947 1239 Size: 268 1524 Fragments arranged by size: 1524 1299 787 629 597 393 351 268 228 156 EcoO109I rG'GnC_Cy Cuts at: 2122 2274 2557 2122 Size: 152 283 5797 Fragments arranged by size: 5797 283 152 EcoRI G'AATT_C Cuts at: 2985 3541 3919 2985 Size: 556 378 5298 Fragments arranged by size: 5298 556 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2752 2752 Size: 6232 FspI TGC'GCA Cuts at: 2138 4738 2138 Size: 2600 3632 FspAI rTGC'GCAy Cuts at: 2138 2138 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 2045 2082 3356 5379 1678 Size: 367 37 1274 2023 2531 Fragments arranged by size: 2531 2023 1274 367 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2321 2353 3481 3513 4873 Size: 32 135 32 749 32 1128 32 1360 Cuts at: 4873 4905 4947 4979 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1360 1128 749 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2734 3576 3822 669 Size: 988 281 796 842 246 3079 Fragments arranged by size: 3079 988 842 796 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6232 HpaI GTT'AAC Cuts at: 3822 3822 Size: 6232 KpnI G_GTAC'C Cuts at: 3557 3557 Size: 6232 MfeI C'AATT_G Cuts at: 3831 3831 Size: 6232 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2670 5346 5530 1305 Size: 1365 2676 184 2007 Fragments arranged by size: 2676 2007 1365 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4410 Fragments arranged by size: 4410 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2090 2408 4190 4549 4708 518 Size: 648 924 318 1782 359 159 2042 Fragments arranged by size: 2042 1782 924 648 359 318 159 MspA1I CmG'CkG Cuts at: 1606 3172 3241 3304 3648 4325 5473 5718 1606 Size: 1566 69 63 344 677 1148 245 2120 Fragments arranged by size: 2120 1566 1148 677 344 245 69 63 NcoI C'CATG_G Cuts at: 513 1065 2004 3267 513 Size: 552 939 1263 3478 Fragments arranged by size: 3478 1263 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6232 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6232 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 2170 2599 3590 5135 1132 Size: 97 941 429 991 1545 2229 Fragments arranged by size: 2229 1545 991 941 429 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6232 PciI A'CATG_T Cuts at: 2166 5131 2166 Size: 2965 3267 PflMI CCAn_nnn'nTGG Cuts at: 1108 2704 1108 Size: 1596 4636 PfoI T'CCnGG_A Cuts at: 3117 3117 Size: 6232 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4301 4333 5814 5846 4301 Size: 32 1481 32 4687 Fragments arranged by size: 4687 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2122 2557 2122 Size: 435 5797 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6232 PsiI TTA'TAA Cuts at: 3802 3802 Size: 6232 PspOMI G'GGCC_C Cuts at: 2273 2273 Size: 6232 PspXI vC'TCGA_Gb Cuts at: 2345 2345 Size: 6232 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2276 2308 2276 Size: 32 6200 PstI C_TGCA'G Cuts at: 830 3008 3171 3519 3584 830 Size: 2178 163 348 65 3478 Fragments arranged by size: 3478 2178 348 163 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6232 PvuII CAG'CTG Cuts at: 3172 3304 3172 Size: 132 6100 SacI G_AGCT'C Cuts at: 2637 2637 Size: 6232 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SapI GCTCTTCn'nnn_ Cuts at: 2663 2663 Size: 6232 SbfI CC_TGCA'GG Cuts at: 3008 3584 3008 Size: 576 5656 ScaI AGT'ACT Cuts at: 2585 4480 6192 2585 Size: 1895 1712 2625 Fragments arranged by size: 2625 1895 1712 SfcI C'TryA_G Cuts at: 826 1045 2827 3004 3167 3515 3580 4715 5396 Size: 219 1782 177 163 348 65 1135 681 Cuts at: 5396 5587 826 Size: 191 1471 Fragments arranged by size: 1782 1471 1135 681 348 219 191 177 163 65 SfiI GGCCn_nnn'nGGCC Cuts at: 3467 3467 Size: 6232 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 3559 1872 Size: 1687 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2126 2345 4336 5237 5499 820 Size: 197 926 183 219 1991 901 262 1553 Fragments arranged by size: 1991 1553 926 901 262 219 197 183 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 3590 1132 Size: 2458 3774 SspI AAT'ATT Cuts at: 5 52 4156 5 Size: 47 4104 2081 Fragments arranged by size: 4104 2081 47 StuI AGG'CCT Cuts at: 1517 2840 3470 1517 Size: 1323 630 4279 Fragments arranged by size: 4279 1323 630 StyI C'CwwG_G Cuts at: 513 1065 2004 2484 3267 3318 3501 3671 513 Size: 552 939 480 783 51 183 170 3074 Fragments arranged by size: 3074 939 783 552 480 183 170 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2420 2522 2965 4259 4276 4429 4614 Size: 1255 840 102 443 1294 17 153 185 Cuts at: 4614 5059 325 Size: 445 1498 Fragments arranged by size: 1498 1294 1255 840 445 443 185 153 102 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2283 2583 2862 Size: 274 80 33 51 1443 306 300 279 Cuts at: 2862 4478 6190 96 Size: 1616 1712 138 Fragments arranged by size: 1712 1616 1443 306 300 279 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6192 6224 6192 Size: 32 6200 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6232 XbaI T'CTAG_A Cuts at: 3568 3568 Size: 6232 XhoI C'TCGA_G Cuts at: 1943 2345 1943 Size: 402 5830 XmaI C'CCGG_G Cuts at: 1870 3557 1870 Size: 1687 4545 XmnI GAAnn'nnTTC Cuts at: 4361 4361 Size: 6232 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2218 4040 276 Size: 53 83 186 936 684 1822 2468 Fragments arranged by size: 2468 1822 936 684 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI RsrII SacII SanDI SexAI SfoI SrfI SwaI XcmI