(Linear) (Six-base) MAPSORT of: KIEE2241.seq Check: 2715 from: 1 to: 1527 KIEE2241.orf With 182 enzymes: SgfI * February 19, 2021 20:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 243 1006 1527 Size: 243 763 521 Fragments arranged by size: 763 521 243 AatII G_ACGT'C Cuts at: 0 215 1527 Size: 215 1312 AccI GT'mk_AC Cuts at: 0 200 1527 Size: 200 1327 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 484 1527 Size: 256 228 1043 Fragments arranged by size: 1043 256 228 AflII C'TTAA_G Cuts at: 0 121 1527 Size: 121 1406 AflIII A'CryG_T Cuts at: 0 161 275 1527 Size: 161 114 1252 Fragments arranged by size: 1252 161 114 AhdI GACnn_n'nnGTC Cuts at: 0 330 1527 Size: 330 1197 AlwNI CAG_nnn'CTG Cuts at: 0 998 1527 Size: 998 529 ApaI G_GGCC'C Cuts at: 0 272 1527 Size: 272 1255 ApoI r'AATT_y Cuts at: 0 980 1527 Size: 980 547 AvaI C'yCGr_G Cuts at: 0 340 1527 Size: 340 1187 BamHI G'GATC_C Cuts at: 0 689 1527 Size: 689 838 BanI G'GyrC_C Cuts at: 0 93 1527 Size: 93 1434 BanII G_rGCy'C Cuts at: 0 272 632 653 1441 1504 1527 Size: 272 360 21 788 63 23 Fragments arranged by size: 788 360 272 63 23 21 BclI T'GATC_A Cuts at: 0 758 1527 Size: 758 769 BglI GCCn_nnn'nGGC Cuts at: 0 1462 1527 Size: 1462 65 BlpI GC'TnA_GC Cuts at: 0 626 718 1168 1527 Size: 626 92 450 359 Fragments arranged by size: 626 450 359 92 Bme1580I G_kGCm'C Cuts at: 0 272 1014 1527 Size: 272 742 513 Fragments arranged by size: 742 513 272 BmrI ACTGGGnnnn_n' Cuts at: 0 137 1217 1326 1527 Size: 137 1080 109 201 Fragments arranged by size: 1080 201 137 109 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1119 1527 Size: 1119 408 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 835 991 1527 Size: 835 156 536 Fragments arranged by size: 835 536 156 BsaAI yAC'GTr Cuts at: 0 276 1527 Size: 276 1251 BsaBI GATnn'nnATC Cuts at: 0 178 1527 Size: 178 1349 BsaHI Gr'CG_yC Cuts at: 0 44 212 1527 Size: 44 168 1315 Fragments arranged by size: 1315 168 44 BsaWI w'CCGG_w Cuts at: 0 1391 1527 Size: 1391 136 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1413 1443 1476 1506 1527 Size: 1413 30 33 30 21 Fragments arranged by size: 1413 33 30 30 21 BseYI C'CCAG_C Cuts at: 0 23 39 69 223 1350 1527 Size: 23 16 30 154 1127 177 Fragments arranged by size: 1127 177 154 30 23 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1066 1527 Size: 1066 461 BsiHKAI G_wGCw'C Cuts at: 0 632 1527 Size: 632 895 BsmI GAATG_Cn' Cuts at: 0 484 738 1527 Size: 484 254 789 Fragments arranged by size: 789 484 254 Bsp1286I G_dGCh'C Cuts at: 0 272 632 653 1014 1441 1504 1527 Size: 272 360 21 361 427 63 23 Fragments arranged by size: 427 361 360 272 63 23 21 BspEI T'CCGG_A Cuts at: 0 1391 1527 Size: 1391 136 BspHI T'CATG_A Cuts at: 0 80 215 473 512 794 1527 Size: 80 135 258 39 282 733 Fragments arranged by size: 733 282 258 135 80 39 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 105 243 992 1006 1527 Size: 105 138 749 14 521 Fragments arranged by size: 749 521 138 105 14 BsrBI CCG'CTC Cuts at: 0 1256 1527 Size: 1256 271 BsrGI T'GTAC_A Cuts at: 0 278 857 1527 Size: 278 579 670 Fragments arranged by size: 670 579 278 BssHII G'CGCG_C Cuts at: 0 19 1527 Size: 19 1508 BssSI C'ACGA_G Cuts at: 0 697 1527 Size: 697 830 BstXI CCAn_nnnn'nTGG Cuts at: 0 417 1527 Size: 417 1110 BstYI r'GATC_y Cuts at: 0 173 230 689 1527 Size: 173 57 459 838 Fragments arranged by size: 838 459 173 57 BtgI C'CryG_G Cuts at: 0 378 1262 1527 Size: 378 884 265 Fragments arranged by size: 884 378 265 DraIII CAC_nnn'GTG Cuts at: 0 699 1527 Size: 699 828 DrdI GACnn_nn'nnGTC Cuts at: 0 400 804 1527 Size: 400 404 723 Fragments arranged by size: 723 404 400 EaeI y'GGCC_r Cuts at: 0 784 1527 Size: 784 743 EarI CTCTTCn'nnn_ Cuts at: 0 658 1283 1527 Size: 658 625 244 Fragments arranged by size: 658 625 244 EcoICRI GAG'CTC Cuts at: 0 630 1527 Size: 630 897 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 484 835 991 1527 Size: 256 228 351 156 536 Fragments arranged by size: 536 351 256 228 156 EcoO109I rG'GnC_Cy Cuts at: 0 117 269 552 1527 Size: 117 152 283 975 Fragments arranged by size: 975 283 152 117 EcoRI G'AATT_C Cuts at: 0 980 1527 Size: 980 547 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 747 1527 Size: 747 780 FspI TGC'GCA Cuts at: 0 133 1527 Size: 133 1394 FspAI rTGC'GCAy Cuts at: 0 133 1527 Size: 133 1394 HaeII r_GCGC'y Cuts at: 0 40 77 1351 1527 Size: 40 37 1274 176 Fragments arranged by size: 1274 176 40 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 316 348 1476 1508 1527 Size: 316 32 1128 32 19 Fragments arranged by size: 1128 316 32 32 19 HincII GTy'rAC Cuts at: 0 729 1527 Size: 729 798 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 665 1527 Size: 665 862 MslI CAynn'nnrTG Cuts at: 0 85 403 1527 Size: 85 318 1124 Fragments arranged by size: 1124 318 85 MspA1I CmG'CkG Cuts at: 0 1167 1236 1299 1527 Size: 1167 69 63 228 Fragments arranged by size: 1167 228 69 63 NcoI C'CATG_G Cuts at: 0 1262 1527 Size: 1262 265 NspI r_CATG'y Cuts at: 0 165 594 1527 Size: 165 429 933 Fragments arranged by size: 933 429 165 PciI A'CATG_T Cuts at: 0 161 1527 Size: 161 1366 PflMI CCAn_nnn'nTGG Cuts at: 0 699 1527 Size: 699 828 PfoI T'CCnGG_A Cuts at: 0 1112 1527 Size: 1112 415 PpuMI rG'GwC_Cy Cuts at: 0 117 552 1527 Size: 117 435 975 Fragments arranged by size: 975 435 117 PspOMI G'GGCC_C Cuts at: 0 268 1527 Size: 268 1259 PspXI vC'TCGA_Gb Cuts at: 0 340 1527 Size: 340 1187 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 271 303 1527 Size: 271 32 1224 Fragments arranged by size: 1224 271 32 PstI C_TGCA'G Cuts at: 0 1003 1166 1514 1527 Size: 1003 163 348 13 Fragments arranged by size: 1003 348 163 13 PvuII CAG'CTG Cuts at: 0 1167 1299 1527 Size: 1167 132 228 Fragments arranged by size: 1167 228 132 SacI G_AGCT'C Cuts at: 0 632 1527 Size: 632 895 SapI GCTCTTCn'nnn_ Cuts at: 0 658 1527 Size: 658 869 SbfI CC_TGCA'GG Cuts at: 0 1003 1527 Size: 1003 524 ScaI AGT'ACT Cuts at: 0 580 1527 Size: 580 947 SfcI C'TryA_G Cuts at: 0 822 999 1162 1510 1527 Size: 822 177 163 348 17 Fragments arranged by size: 822 348 177 163 17 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1462 1527 Size: 1462 65 SmlI C'TyrA_G Cuts at: 0 121 340 1527 Size: 121 219 1187 Fragments arranged by size: 1187 219 121 StuI AGG'CCT Cuts at: 0 835 1465 1527 Size: 835 630 62 Fragments arranged by size: 835 630 62 StyI C'CwwG_G Cuts at: 0 479 1262 1313 1496 1527 Size: 479 783 51 183 31 Fragments arranged by size: 783 479 183 51 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 415 517 960 1527 Size: 415 102 443 567 Fragments arranged by size: 567 443 415 102 TatI w'GTAC_w Cuts at: 0 278 578 857 1527 Size: 278 300 279 670 Fragments arranged by size: 670 300 279 278 XhoI C'TCGA_G Cuts at: 0 340 1527 Size: 340 1187 ZraI GAC'GTC Cuts at: 0 213 1527 Size: 213 1314 Enzymes that do cut: AarI AatII AccI AcuI AflII AflIII AhdI AlwNI ApaI ApoI AvaI BamHI BanI BanII BclI BglI BlpI Bme1580I BmrI BplI BpmI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstXI BstYI BtgI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII MmeI MslI MspA1I NcoI NspI PciI PflMI PfoI PpuMI PspOMI PspXI PsrI PstI PvuII SacI SapI SbfI ScaI SfcI SfiI SmlI StuI StyI TaqII TatI XhoI ZraI Enzymes that do not cut: Acc65I AclI AfeI AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI Bpu10I BpuEI BsaI BseRI BsiEI BsiWI BsmBI BsrDI BsrFI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI EagI EciI EcoNI EcoRV FseI HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI PvuI RsrII SacII SalI SanDI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI XmaI XmnI