################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## Acc65I AclI AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvrII BalI BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BglII BlnI BpuAI BsaI BseRI BsiEI BsiWI BsmBI BspDI BsrDI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FseI HindIII HpaI KasI KpnI KspI MaeI MfeI MluI MluNI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 211 GACGTC GACGT^C AccI 199 GTCTAC GT^[AC][GT]AC AccIII 1391 TCCGGA T^CCGGA AcsI 980 GAATTC [GA]^AATT[CT] AflII 121 CTTAAG C^TTAAG AhdI 325 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 993 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1405 CCTGAGG CC^T[ACGT]AGG ApaI 268 GGGCCC GGGCC^C ApoI 980 GAATTC [GA]^AATT[CT] AspEI 325 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 628 GAGCTC G[AT]GC[AT]^C AvaI 340 CTCGAG C^[CT]CG[GA]G AviII 131 TGCGCA TGC^GCA BamHI 689 GGATCC G^GATCC BanI 93 GGTGCC G^G[CT][GA]CC BclI 758 TGATCA T^GATCA BfrI 121 CTTAAG C^TTAAG BsaAI 274 TACGTG [CT]AC^GT[GA] BsaBI 174 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1391 TCCGGA [AT]^CCGG[AT] BseAI 1391 TCCGGA T^CCGGA BsgI 1081 CTGCAC GTGCAG(16/14) BsiHKAI 628 GAGCTC G[AT]GC[AT]^C BsmAI 1273 GAGAC GTCTC(1/5) BsoBI 340 CTCGAG C^[CT]CG[GA]G BspEI 1391 TCCGGA T^CCGGA BspLU11I 161 ACATGT A^CATGT BsrBI 1254 GAGCGG CCGCTC(-3/-3) BsrFI 1448 ACCGGC [GA]^CCGG[CT] BssHII 19 GCGCGC G^CGCGC BssSI 697 CTCGTG CACGAG(-5/-1) BstXI 410 CCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1405 CCTGAGG CC^T[ACGT]AGG Cfr10I 1448 ACCGGC [GA]^CCGG[CT] DraIII 694 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 325 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1405 CCTGAGG CC^T[ACGT]AGG EcoRI 980 GAATTC G^AATTC FbaI 758 TGATCA T^GATCA FspI 131 TGCGCA TGC^GCA HincII 727 GTTGAC GT[CT]^[GA]AC HindII 727 GTTGAC GT[CT]^[GA]AC MamI 174 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1391 TCCGGA T^CCGGA MseI 122 TTAA T^TAA NcoI 1262 CCATGG C^CATGG PaeR7I 340 CTCGAG C^TCGAG PflMI 693 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 628 GAGCTC GAGCT^C SapI 651 GCTCTTC GCTCTTC(1/4) SbfI 998 CCTGCAGG CCTGCA^GG ScaI 578 AGTACT AGT^ACT SfiI 1442 GGCCTGACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 998 CCTGCAGG CCTGCA^GG Tru9I 122 TTAA T^TAA Tsp509I 981 AATT ^AATT Van91I 693 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 340 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 43 GGCGTC G[GA]^CG[CT]C 211 GACGTC AflIII 161 ACATGT A^C[GA][CT]GT 275 ACGTGT BglI 1421 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1443 GCCTGACCGGC BpmI 814 CTGGAG CTGGAG(16/14) 970 CTGGAG BsaHI 43 GGCGTC G[GA]^CG[CT]C 211 GACGTC BsmFI 375 GTCCC GGGAC(10/14) 1372 GTCCC BsmI 484 GCATTC GAATGC(1/-1) 738 GCATTC Bsp1407I 278 TGTACA T^GTACA 857 TGTACA BsrGI 278 TGTACA T^GTACA 857 TGTACA DrdI 394 GACTACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 798 GACTCCTGAGTC DsaI 378 CCACGG C^C[GA][CT]GG 1262 CCATGG EaeI 784 TGGCCG [CT]^GGCC[GA] 1433 TGGCCG EarI 652 CTCTTC CTCTTC(1/4) 1277 CTCTTC HgaI 44 GCGTC GACGC(5/10) 1358 GCGTC Hin1I 43 GGCGTC G[GA]^CG[CT]C 211 GACGTC Ksp632I 652 CTCTTC CTCTTC(1/4) 1277 CTCTTC MaeIII 1072 GTGAC ^GT[ACGT]AC 1129 GTCAC MslI 81 CATGATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 399 CATCGTCATG NspI 161 ACATGT [GA]CATG^[CT] 590 GCATGT PstI 999 CTGCAG CTGCA^G 1162 CTGCAG PvuII 1165 CAGCTG CAG^CTG 1297 CAGCTG SmlI 121 CTTAAG C^T[CT][GA]AG 340 CTCGAG SspBI 278 TGTACA T^GTACA 857 TGTACA StuI 833 AGGCCT AGG^CCT 1428 AGGCCT TaqI 137 TCGA T^CGA 341 TCGA TfiI 564 GAATC G^A[AT]TC 1136 GAATC Tsp45I 1072 GTGAC ^GT[GC]AC 1129 GTCAC TthHB8I 137 TCGA T^CGA 341 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BanII BbvI BcnI BlpI BmyI Bpu1102I BsaJI BsiYI BslI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI TspRI XhoII