(Linear) (Six-base) MAPSORT of: KIEE2798.seq Check: 9627 from: 1 to: 1770 KIEE2798.orf With 182 enzymes: SgfI * August 16, 2021 14:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1176 1770 Size: 1176 594 AccI GT'mk_AC Cuts at: 0 467 1770 Size: 467 1303 AlwNI CAG_nnn'CTG Cuts at: 0 1632 1770 Size: 1632 138 ApaI G_GGCC'C Cuts at: 0 54 630 738 900 954 1770 Size: 54 576 108 162 54 816 Fragments arranged by size: 816 576 162 108 54 54 BamHI G'GATC_C Cuts at: 0 680 1770 Size: 680 1090 BanI G'GyrC_C Cuts at: 0 1543 1770 Size: 1543 227 BanII G_rGCy'C Cuts at: 0 54 269 630 738 900 954 1770 Size: 54 215 361 108 162 54 816 Fragments arranged by size: 816 361 215 162 108 54 54 BbsI GAAGACnn'nnnn_ Cuts at: 0 1208 1770 Size: 1208 562 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 457 491 1770 Size: 457 34 1279 Fragments arranged by size: 1279 457 34 BfrBI ATG'CAT Cuts at: 0 3 1770 Size: 3 1767 BglI GCCn_nnn'nGGC Cuts at: 0 236 430 1702 1770 Size: 236 194 1272 68 Fragments arranged by size: 1272 236 194 68 BlpI GC'TnA_GC Cuts at: 0 118 1770 Size: 118 1652 Bme1580I G_kGCm'C Cuts at: 0 54 630 738 900 954 1548 1770 Size: 54 576 108 162 54 594 222 Fragments arranged by size: 594 576 222 162 108 54 54 BmgBI CAC'GTC Cuts at: 0 13 1770 Size: 13 1757 BmrI ACTGGGnnnn_n' Cuts at: 0 55 1454 1770 Size: 55 1399 316 Fragments arranged by size: 1399 316 55 BsaI GGTCTCn'nnnn_ Cuts at: 0 643 805 1021 1770 Size: 643 162 216 749 Fragments arranged by size: 749 643 216 162 BsaHI Gr'CG_yC Cuts at: 0 257 1770 Size: 257 1513 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1761 1770 Size: 1761 9 BseYI C'CCAG_C Cuts at: 0 499 553 607 985 1297 1459 1770 Size: 499 54 54 378 312 162 311 Fragments arranged by size: 499 378 312 311 162 54 54 BsmI GAATG_Cn' Cuts at: 0 1590 1770 Size: 1590 180 Bsp1286I G_dGCh'C Cuts at: 0 54 269 630 738 900 954 1548 1770 Size: 54 215 361 108 162 54 594 222 Fragments arranged by size: 594 361 222 215 162 108 54 54 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1176 1770 Size: 1176 594 BsrDI GCAATG_nn' Cuts at: 0 129 1770 Size: 129 1641 BsrFI r'CCGG_y Cuts at: 0 235 694 1770 Size: 235 459 1076 Fragments arranged by size: 1076 459 235 BssSI C'ACGA_G Cuts at: 0 1345 1770 Size: 1345 425 BstXI CCAn_nnnn'nTGG Cuts at: 0 313 856 1459 1558 1770 Size: 313 543 603 99 212 Fragments arranged by size: 603 543 313 212 99 BstYI r'GATC_y Cuts at: 0 680 1654 1770 Size: 680 974 116 Fragments arranged by size: 974 680 116 BtgI C'CryG_G Cuts at: 0 273 306 348 633 795 1011 1107 1119 Size: 273 33 42 285 162 216 96 12 Cuts at: 1119 1770 Size: 651 Fragments arranged by size: 651 285 273 216 162 96 42 33 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 89 1770 Size: 89 1681 DrdI GACnn_nn'nnGTC Cuts at: 0 256 1210 1426 1770 Size: 256 954 216 344 Fragments arranged by size: 954 344 256 216 EaeI y'GGCC_r Cuts at: 0 68 345 456 510 564 618 672 726 Size: 68 277 111 54 54 54 54 54 Cuts at: 726 780 834 888 942 996 1050 1104 1158 Size: 54 54 54 54 54 54 54 54 Cuts at: 1158 1266 1374 1482 1536 1560 1770 Size: 108 108 108 54 24 210 Fragments arranged by size: 277 210 111 108 108 108 68 54 54 54 54 54 54 54 54 54 54 54 54 54 54 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 1399 1770 Size: 1399 371 EcoO109I rG'GnC_Cy Cuts at: 0 50 51 147 626 734 896 950 1703 Size: 50 1 96 479 108 162 54 753 Cuts at: 1703 1770 Size: 67 Fragments arranged by size: 753 479 162 108 96 67 54 50 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1673 1770 Size: 1673 97 HincII GTy'rAC Cuts at: 0 357 468 1494 1602 1713 1770 Size: 357 111 1026 108 111 57 Fragments arranged by size: 1026 357 111 111 108 57 HindIII A'AGCT_T Cuts at: 0 340 1531 1770 Size: 340 1191 239 Fragments arranged by size: 1191 340 239 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 227 1356 1770 Size: 227 1129 414 Fragments arranged by size: 1129 414 227 MscI TGG'CCA Cuts at: 0 347 458 512 566 620 674 728 782 Size: 347 111 54 54 54 54 54 54 Cuts at: 782 836 890 944 998 1052 1160 1268 1376 Size: 54 54 54 54 54 108 108 108 Cuts at: 1376 1484 1538 1562 1770 Size: 108 54 24 208 Fragments arranged by size: 347 208 111 108 108 108 108 54 54 54 54 54 54 54 54 54 54 54 54 24 MspA1I CmG'CkG Cuts at: 0 1629 1770 Size: 1629 141 NcoI C'CATG_G Cuts at: 0 306 348 633 795 1011 1107 1119 1770 Size: 306 42 285 162 216 96 12 651 Fragments arranged by size: 651 306 285 216 162 96 42 12 NsiI A_TGCA'T Cuts at: 0 5 1770 Size: 5 1765 NspI r_CATG'y Cuts at: 0 181 1770 Size: 181 1589 PflMI CCAn_nnn'nTGG Cuts at: 0 279 1770 Size: 279 1491 PspOMI G'GGCC_C Cuts at: 0 50 626 734 896 950 1770 Size: 50 576 108 162 54 820 Fragments arranged by size: 820 576 162 108 54 50 PstI C_TGCA'G Cuts at: 0 480 534 588 1583 1770 Size: 480 54 54 995 187 Fragments arranged by size: 995 480 187 54 54 PvuII CAG'CTG Cuts at: 0 1629 1770 Size: 1629 141 SalI G'TCGA_C Cuts at: 0 466 1770 Size: 466 1304 SfcI C'TryA_G Cuts at: 0 476 530 584 1579 1770 Size: 476 54 54 995 191 Fragments arranged by size: 995 476 191 54 54 SphI G_CATG'C Cuts at: 0 181 1770 Size: 181 1589 StuI AGG'CCT Cuts at: 0 1135 1220 1770 Size: 1135 85 550 Fragments arranged by size: 1135 550 85 StyI C'CwwG_G Cuts at: 0 306 348 633 795 1011 1107 1119 1215 Size: 306 42 285 162 216 96 12 96 Cuts at: 1215 1431 1485 1539 1770 Size: 216 54 54 231 Fragments arranged by size: 306 285 231 216 216 162 96 96 54 54 42 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1698 1770 Size: 1698 72 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 176 208 1508 1540 1770 Size: 176 32 1300 32 230 Fragments arranged by size: 1300 230 176 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 853 1555 1633 1770 Size: 853 702 78 137 Fragments arranged by size: 853 702 137 78 XmnI GAAnn'nnTTC Cuts at: 0 202 1770 Size: 202 1568 Enzymes that do cut: AarI AccI AlwNI ApaI BamHI BanI BanII BbsI BcgI BfrBI BglI BlpI Bme1580I BmgBI BmrI BsaI BsaHI BseRI BseYI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstXI BstYI BtgI BtgZI DrdI EaeI EcoNI EcoO109I FalI HincII HindIII MmeI MscI MspA1I NcoI NsiI NspI PflMI PspOMI PstI PvuII SalI SfcI SphI StuI StyI TaqII TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BciVI BclI BglII BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HpaI KasI KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI ZraI