Restriction map for
pFN21AE2605
##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BbrPI BcgI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I CelII ClaI CpoI
DraI DraIII Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KspI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SfiI SfuI SgrAI SnaBI
SspBI SspI SwaI Tru9I Tsp509I
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 329 GGTACC G^GTACC
AccI 619 GTCTAC GT^[AC][GT]AC
AccII 265 CGCG CG^CG
AcyI 610 GGCGCC G[GA]^CG[CT]C
AflIII 341 ACATGT A^C[GA][CT]GT
Alw44I 1351 GTGCAC G^TGCAC
AocI 605 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1481 AGCGCT AGC^GCT
ApaLI 1351 GTGCAC G^TGCAC
Asp718I 329 GGTACC G^GTACC
BamHI 158 GGATCC G^GATCC
BbeI 610 GGCGCC GGCGC^C
BciVI 711 GGATAC GTATCC(6/5)
BglI 1373 GCCGCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1223 AGATCT A^GATCT
BsaAI 1525 TACGTG [CT]AC^GT[GA]
BsaHI 610 GGCGCC G[GA]^CG[CT]C
BsiEI 1371 CGGCCG CG[GA][CT]^CG
BsmBI 361 GAGACG CGTCTC(1/5)
BspHI 1043 TCATGA T^CATGA
BspLU11I 341 ACATGT A^CATGT
BsrBI 481 CCGCTC CCGCTC(-3/-3)
BsrFI 1363 GCCGGC [GA]^CCGG[CT]
BssSI 645 CACGAG CACGAG(-5/-1)
BstUI 265 CGCG CG^CG
Bsu36I 605 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1363 GCCGGC [GA]^CCGG[CT]
DrdI 1255 GACCATGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1371 CGGCCG C^GGCCG
EarI 1758 GAAGAG CTCTTC(1/4)
EclXI 1371 CGGCCG C^GGCCG
Eco47III 1481 AGCGCT AGC^GCT
Eco52I 1371 CGGCCG C^GGCCG
Eco81I 605 CCTCAGG CC^T[ACGT]AGG
Hin1I 610 GGCGCC G[GA]^CG[CT]C
KasI 610 GGCGCC G^GCGCC
KpnI 329 GGTACC GGTAC^C
Ksp632I 1758 GAAGAG CTCTTC(1/4)
MvnI 265 CGCG CG^CG
NaeI 1363 GCCGGC GCC^GGC
NarI 610 GGCGCC GG^CGCC
NgoMIV 1363 GCCGGC G^CCGGC
PflMI 780 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 928 CAGCTG CAG^CTG
RcaI 1043 TCATGA T^CATGA
SapI 1758 GAAGAGC GCTCTTC(1/4)
ScaI 230 AGTACT AGT^ACT
SexAI 566 ACCTGGT A^CC[AT]GGT
SfoI 610 GGCGCC GGC^GCC
SmaI 889 CCCGGG CCC^GGG
SpeI 316 ACTAGT A^CTAGT
SphI 1106 GCATGC GCATG^C
TfiI 1215 GATTC G^A[AT]TC
Van91I 780 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 889 CCCGGG C^CCGGG
XmaI 889 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AlwNI 781 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1237 CAGTACCTG
ApaI 1123 GGGCCC GGGCC^C
1339 GGGCCC
AspHI 445 GTGCTC G[AT]GC[AT]^C
1351 GTGCAC
BalI 336 TGGCCA TGG^CCA
1529 TGGCCA
BbsI 585 GAAGAC GAAGAC(2/6)
1455 GAAGAC
BbvCI 414 CCTCAGC CCTCAGC(-5/-2)
1287 CCTCAGC
BcnI 889 CCCGG CC^[GC]GG
890 CCGGG
BfaI 317 CTAG C^TAG
934 CTAG
BpuAI 585 GAAGAC GAAGAC(2/6)
1455 GAAGAC
BsgI 1699 CTGCAC GTGCAG(16/14)
1495 GTGCAG
BsiHKAI 445 GTGCTC G[AT]GC[AT]^C
1351 GTGCAC
BsmI 654 GAATGC GAATGC(1/-1)
1638 GAATGC
BspMI 623 ACCTGC ACCTGC(4/8)
1697 ACCTGC
BstEII 144 GGTGACC G^GT[ACGT]ACC
489 GGTCACC
BstPI 144 GGTGACC G^GT[ACGT]ACC
489 GGTCACC
BstYI 158 GGATCC [GA]^GATC[CT]
1223 AGATCT
EcoNI 48 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1330 CCTCACGCAGG
EcoO65I 144 GGTGACC G^GT[ACGT]ACC
489 GGTCACC
MaeI 317 CTAG C^TAG
934 CTAG
MaeII 394 ACGT A^CGT
1526 ACGT
MflI 158 GGATCC [GA]^GATC[CT]
1223 AGATCT
MluNI 336 TGGCCA TGG^CCA
1529 TGGCCA
MscI 336 TGGCCA TGG^CCA
1529 TGGCCA
NciI 889 CCCGG CC^[GC]GG
890 CCGGG
NcoI 333 CCATGG C^CATGG
1257 CCATGG
PpuMI 1262 GGGTCCC [GA]G^G[AT]CC[CT]
1571 AGGTCCT
SbfI 255 CCTGCAGG CCTGCA^GG
864 CCTGCAGG
SfaNI 37 GATGC GCATC(5/9)
1367 GCATC
Sse8387I 255 CCTGCAGG CCTGCA^GG
864 CCTGCAGG
StuI 602 AGGCCT AGG^CCT
1652 AGGCCT
TaqI 551 TCGA T^CGA
1397 TCGA
TthHB8I 551 TCGA T^CGA
1397 TCGA
XhoII 158 GGATCC [GA]^GATC[CT]
1223 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanI BanII BbvI BmyI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfcI SmlI
StyI TseI Tsp45I TspRI XcmI