Restriction map for
pFN21AE2605
(Circular) (Six-base) MAPSORT of: pFN21AE2605.seq Check: 7875 from: 1 to: 6490
pFN21AE2605 6490 bp
With 182 enzymes: SgfI *
January 12, 2016 11:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4300 278
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2625 3833 1937
Size: 688 1208 4594
Fragments arranged by size:
4594 1208 688
Acc65I G'GTAC_C
Cuts at: 2334 3811 2334
Size: 1477 5013
AclI AA'CG_TT
Cuts at: 1563 4617 4990 1563
Size: 3054 373 3063
Fragments arranged by size:
3063 3054 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4553 5937 6205 1632
Size: 2921 1384 268 1917
Fragments arranged by size:
2921 1917 1384 268
AfeI AGC'GCT
Cuts at: 3488 3488
Size: 6490
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6293
AflIII A'CryG_T
Cuts at: 1203 2346 5389 6366 1203
Size: 1143 3043 977 1327
Fragments arranged by size:
3043 1327 1143 977
AgeI A'CCGG_T
Cuts at: 5321 5321
Size: 6490
AhdI GACnn_n'nnGTC
Cuts at: 5219 5219
Size: 6490
AleI CACnn'nnGTG
Cuts at: 1166 2654 1166
Size: 1488 5002
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6490
AlwNI CAG_nnn'CTG
Cuts at: 2791 3247 4233 5805 2791
Size: 456 986 1572 3476
Fragments arranged by size:
3476 1572 986 456
ApaI G_GGCC'C
Cuts at: 3132 3348 3132
Size: 216 6274
ApaLI G'TGCA_C
Cuts at: 3356 4547 5703 3356
Size: 1191 1156 4143
Fragments arranged by size:
4143 1191 1156
ApoI r'AATT_y
Cuts at: 1493 3799 4025 4177 6286 1493
Size: 2306 226 152 2109 1697
Fragments arranged by size:
2306 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5044 160
Size: 872 4012 1606
Fragments arranged by size:
4012 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
AvaI C'yCGr_G
Cuts at: 1870 1943 2894 3057 3101 3490 3815 1870
Size: 73 951 163 44 389 325 4545
Fragments arranged by size:
4545 951 389 325 163 73 44
BamHI G'GATC_C
Cuts at: 2163 3820 4187 2163
Size: 1657 367 4466
Fragments arranged by size:
4466 1657 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2276 2334 2615 3386 3811
Size: 325 217 627 489 58 281 771 425
Cuts at: 3811 5266 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 771 627 489 425 325 281
217 58
BanII G_rGCy'C
Cuts at: 1391 2394 3132 3348 3809 1391
Size: 1003 738 216 461 4072
Fragments arranged by size:
4072 1003 738 461 216
BbeI G_GCGC'C
Cuts at: 2619 2619
Size: 6490
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2597 3467 928
Size: 971 698 870 3951
Fragments arranged by size:
3951 971 870 698
BbvCI CC'TCA_GC
Cuts at: 2420 3293 2420
Size: 873 5617
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4681 4715 1903
Size: 34 2744 34 3678
Fragments arranged by size:
3678 2744 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2710 4382 5592 2710
Size: 1672 1210 3608
Fragments arranged by size:
3608 1672 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6490
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3384 5101 136
Size: 107 122 71 631 605 1712 1717 1525
Fragments arranged by size:
1717 1712 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3228 6485 3228
Size: 3257 3233
BlpI GC'TnA_GC
Cuts at: 3907 3907
Size: 6490
Bme1580I G_kGCm'C
Cuts at: 1790 3132 3348 3360 4551 5707 1790
Size: 1342 216 12 1191 1156 2573
Fragments arranged by size:
2573 1342 1191 1156 216 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6490
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2091 3267 3538
Size: 416 53 83 399 24 674 1176 271
Cuts at: 3538 4221 5179 442
Size: 683 958 1753
Fragments arranged by size:
1753 1176 958 683 674 416 399 271
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6490
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2626 3439 2626
Size: 813 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2451 3008 3593 5150 1239
Size: 1212 557 585 1557 2579
Fragments arranged by size:
2579 1557 1212 585 557
Bpu10I CC'TnA_GC
Cuts at: 2420 2489 3248 3293 2420
Size: 69 759 45 5617
Fragments arranged by size:
5617 759 69 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2042 2276 3764 4615 5480 5778 2042
Size: 234 1488 851 865 298 2754
Fragments arranged by size:
2754 1488 865 851 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2019 2042 2543 3199 3207 5153 882
Size: 617 520 23 501 656 8 1946 2219
Fragments arranged by size:
2219 1946 656 617 520 501 23 8
BsaAI yAC'GTr
Cuts at: 493 1204 3532 4281 493
Size: 711 2328 749 2702
Fragments arranged by size:
2702 2328 749 711
BsaBI GATnn'nnATC
Cuts at: 6484 6484
Size: 6490
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2616 4297 4679 5314
Size: 53 83 186 936 1083 1681 382 635
Cuts at: 5314 275
Size: 1451
Fragments arranged by size:
1681 1451 1083 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4923 5321 5595 5742 6153 4923
Size: 398 274 147 411 5260
Fragments arranged by size:
5260 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2424 2454 3500 3530 3593
Size: 30 165 30 1021 30 1046 30 63
Cuts at: 3593 3623 3677 3707 1178
Size: 30 54 30 3961
Fragments arranged by size:
3961 1046 1021 165 63 54 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2618 2756 3740 1186
Size: 1432 138 984 3936
Fragments arranged by size:
3936 1432 984 138
BseYI C'CCAG_C
Cuts at: 3631 5693 6189 3631
Size: 2062 496 3932
Fragments arranged by size:
3932 2062 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3521 3689 1163
Size: 573 1785 168 3964
Fragments arranged by size:
3964 1785 573 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3379 3940 4701 5729 1540
Size: 275 186 1378 561 761 1028 2301
Fragments arranged by size:
2301 1378 1028 761 561 275 186
BsiHKAI G_wGCw'C
Cuts at: 2454 3360 4551 4636 5707 2454
Size: 906 1191 85 1071 3237
Fragments arranged by size:
3237 1191 1071 906 85
BsmI GAATG_Cn'
Cuts at: 2665 3649 4001 4094 2665
Size: 984 352 93 5061
Fragments arranged by size:
5061 984 352 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2360 2360
Size: 6490
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2394 2454 3132 3348 3360 3809 4551
Size: 399 604 60 678 216 12 449 742
Cuts at: 4551 4636 5707 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 678 604 449 399 216
85 60 12
BspEI T'CCGG_A
Cuts at: 6153 6153
Size: 6490
BspHI T'CATG_A
Cuts at: 3048 4379 6109 3048
Size: 1331 1730 3429
Fragments arranged by size:
3429 1730 1331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2637 3711 3845 844
Size: 1793 1074 134 3489
Fragments arranged by size:
3489 1793 1074 134
BsrBI CCG'CTC
Cuts at: 1394 1823 2488 4377 1394
Size: 429 665 1889 3507
Fragments arranged by size:
3507 1889 665 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4985 5159 65
Size: 1183 3737 174 1396
Fragments arranged by size:
3737 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3368 5134 5321 1700
Size: 110 1558 1766 187 2869
Fragments arranged by size:
2869 1766 1558 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6490
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6490
BssSI C'ACGA_G
Cuts at: 2650 4550 5562 2650
Size: 1900 1012 3578
Fragments arranged by size:
3578 1900 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6490
BstBI TT'CG_AA
Cuts at: 5294 5294
Size: 6490
BstEII G'GTnAC_C
Cuts at: 1184 2149 2494 1184
Size: 965 345 5180
Fragments arranged by size:
5180 965 345
BstXI CCAn_nnnn'nTGG
Cuts at: 2351 3436 3472 2351
Size: 1085 36 5369
Fragments arranged by size:
5369 1085 36
BstYI r'GATC_y
Cuts at: 1972 2163 3228 3820 4187 4572 4589 6041 6127
Size: 191 1065 592 367 385 17 1452 86
Cuts at: 6127 6139 6485 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1065 592 385 367 346 191
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2611 2611
Size: 6490
BtgI C'CryG_G
Cuts at: 513 1065 2004 2074 2338 2994 3262 513
Size: 552 939 70 264 656 268 3741
Fragments arranged by size:
3741 939 656 552 268 264 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2953 3523 487
Size: 683 56 1727 570 3454
Fragments arranged by size:
3454 1727 683 570 56
BtsI GCAGTG_nn'
Cuts at: 3358 4006 4800 4820 3358
Size: 648 794 20 5028
Fragments arranged by size:
5028 794 648 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6490
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6455
DraI TTT'AAA
Cuts at: 3794 3794
Size: 6490
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3266 5497 809
Size: 507 1950 2231 1802
Fragments arranged by size:
2231 1950 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2341 2804 3376
Size: 54 1425 50 275 18 511 463 572
Cuts at: 3376 3534 3937 4826 6455 8
Size: 158 403 889 1629 43
Fragments arranged by size:
1629 1425 889 572 511 463 403 275
158 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3376 3937 1537
Size: 275 1564 561 4090
Fragments arranged by size:
4090 1564 561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3758 4420 1353
Size: 2405 662 3423
Fragments arranged by size:
3423 2405 662
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5077 5451 5597 387
Size: 1064 138 3488 374 146 1280
Fragments arranged by size:
3488 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2451 3008 3593 4553 5150 5937 6205
Size: 393 819 557 585 960 597 787 268
Cuts at: 6205 1239
Size: 1524
Fragments arranged by size:
1524 960 819 787 597 585 557 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 3339 2057
Size: 1282 5208
EcoO109I rG'GnC_Cy
Cuts at: 2942 3129 3268 3344 3433 3577 2942
Size: 187 139 76 89 144 5855
Fragments arranged by size:
5855 187 144 139 89 76
EcoRI G'AATT_C
Cuts at: 3799 4177 3799
Size: 378 6112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 6490
FspI TGC'GCA
Cuts at: 4996 4996
Size: 6490
HaeII r_GCGC'y
Cuts at: 1678 2619 3369 3490 5637 1678
Size: 941 750 121 2147 2531
Fragments arranged by size:
2531 2147 941 750 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5131 5163 5205 5237 1373
Size: 32 135 32 3559 32 42 32 2626
Fragments arranged by size:
3559 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3834 4080 669
Size: 988 281 1896 246 3079
Fragments arranged by size:
3079 1896 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6490
HpaI GTT'AAC
Cuts at: 4080 4080
Size: 6490
KasI G'GCGC_C
Cuts at: 2615 2615
Size: 6490
KpnI G_GTAC'C
Cuts at: 2338 3815 2338
Size: 1477 5013
MfeI C'AATT_G
Cuts at: 4089 4089
Size: 6490
MluI A'CGCG_T
Cuts at: 6366 6366
Size: 6490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2667 2925 3585 5604 5788 1305
Size: 1362 258 660 2019 184 2007
Fragments arranged by size:
2019 2007 1362 660 258 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2343 3536 10
Size: 54 1425 343 511 1193 2964
Fragments arranged by size:
2964 1425 1193 511 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2654 3047 3353 3404 3410 3470 3530
Size: 648 1488 393 306 51 6 60 60
Cuts at: 3530 4448 4807 4966 518
Size: 918 359 159 2042
Fragments arranged by size:
2042 1488 918 648 393 359 306 159
60 60 51 6
MspA1I CmG'CkG
Cuts at: 1606 2302 2803 2935 3202 3319 3745 3906 4583
Size: 696 501 132 267 117 426 161 677
Cuts at: 4583 5731 5976 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 696 677 501 426 267 245
161 132 117
NaeI GCC'GGC
Cuts at: 3370 3370
Size: 6490
NarI GG'CG_CC
Cuts at: 2616 2616
Size: 6490
NcoI C'CATG_G
Cuts at: 513 1065 2004 2338 3262 513
Size: 552 939 334 924 3741
Fragments arranged by size:
3741 939 924 552 334
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6490
NgoMIV G'CCGG_C
Cuts at: 3368 3368
Size: 6490
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6490
NotI GC'GGCC_GC
Cuts at: 3937 3937
Size: 6490
NspI r_CATG'y
Cuts at: 1132 1229 2350 3115 3409 3848 5393 1132
Size: 97 1121 765 294 439 1545 2229
Fragments arranged by size:
2229 1545 1121 765 439 294 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6490
PciI A'CATG_T
Cuts at: 2346 5389 2346
Size: 3043 3447
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2791 1108
Size: 1683 4807
PfoI T'CCnGG_A
Cuts at: 3300 3300
Size: 6490
PmeI GTTT'AAAC
Cuts at: 3794 3794
Size: 6490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3757 3789 4559 4591 6072 6104 3757
Size: 32 770 32 1481 32 4143
Fragments arranged by size:
4143 1481 770 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3268 3577 3268
Size: 309 6181
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6490
PsiI TTA'TAA
Cuts at: 4060 4060
Size: 6490
PspOMI G'GGCC_C
Cuts at: 3128 3344 3128
Size: 216 6274
PstI C_TGCA'G
Cuts at: 830 2265 2379 2874 3365 3585 3842 830
Size: 1435 114 495 491 220 257 3478
Fragments arranged by size:
3478 1435 495 491 257 220 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6490
PvuII CAG'CTG
Cuts at: 2935 2935
Size: 6490
SalI G'TCGA_C
Cuts at: 1936 3832 1936
Size: 1896 4594
SanDI GG'GwC_CC
Cuts at: 3268 3268
Size: 6490
SapI GCTCTTCn'nnn_
Cuts at: 3758 3758
Size: 6490
SbfI CC_TGCA'GG
Cuts at: 2265 2874 3842 2265
Size: 609 968 4913
Fragments arranged by size:
4913 968 609
ScaI AGT'ACT
Cuts at: 2237 4738 6450 2237
Size: 2501 1712 2277
Fragments arranged by size:
2501 2277 1712
SexAI A'CCwGG_T
Cuts at: 2571 2571
Size: 6490
SfcI C'TryA_G
Cuts at: 826 1045 2261 2375 2870 3361 3581 3838 4973
Size: 219 1216 114 495 491 220 257 1135
Cuts at: 4973 5654 5845 826
Size: 681 191 1471
Fragments arranged by size:
1471 1216 1135 681 495 491 257 220
219 191 114
SfoI GGC'GCC
Cuts at: 2617 2617
Size: 6490
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6490
SmaI CCC'GGG
Cuts at: 1872 2896 3817 1872
Size: 1024 921 4545
Fragments arranged by size:
4545 1024 921
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2057 2291 3779 4594 5495 5757
Size: 197 926 114 234 1488 815 901 262
Cuts at: 5757 820
Size: 1553
Fragments arranged by size:
1553 1488 926 901 815 262 234 197
114
SnaBI TAC'GTA
Cuts at: 493 4281 493
Size: 3788 2702
SpeI A'CTAG_T
Cuts at: 152 2321 152
Size: 2169 4321
SphI G_CATG'C
Cuts at: 1132 3115 3848 1132
Size: 1983 733 3774
Fragments arranged by size:
3774 1983 733
SspI AAT'ATT
Cuts at: 5 52 4414 5
Size: 47 4362 2081
Fragments arranged by size:
4362 2081 47
StuI AGG'CCT
Cuts at: 1517 2609 3659 1517
Size: 1092 1050 4348
Fragments arranged by size:
4348 1092 1050
StyI C'CwwG_G
Cuts at: 513 1065 2004 2338 2382 2466 2724 3262 3429
Size: 552 939 334 44 84 258 538 167
Cuts at: 3429 3654 3929 513
Size: 225 275 3074
Fragments arranged by size:
3074 939 552 538 334 275 258 225
167 84 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2513 2528 4517 4534 4687 4872 5317
Size: 1255 933 15 1989 17 153 185 445
Cuts at: 5317 325
Size: 1498
Fragments arranged by size:
1989 1498 1255 933 445 185 153 17
15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 3516 4736
Size: 274 80 33 51 1443 258 1281 1220
Cuts at: 4736 6448 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1281 1220 274 258 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2424 2456 6450 6482 2424
Size: 32 3994 32 2432
Fragments arranged by size:
3994 2432 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6490
XbaI T'CTAG_A
Cuts at: 3826 3826
Size: 6490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2468 3061 3433 2468
Size: 593 372 5525
Fragments arranged by size:
5525 593 372
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6490
XmaI C'CCGG_G
Cuts at: 1870 2894 3815 1870
Size: 1024 921 4545
Fragments arranged by size:
4545 1024 921
XmnI GAAnn'nnTTC
Cuts at: 4619 4619
Size: 6490
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4298 276
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SfiI SrfI
SwaI