(Linear) (Six-base) MAPSORT of: KIEE2605.seq Check: 3315 from: 1 to: 1785 KIEE2605.orf With 182 enzymes: SgfI * January 12, 2016 10:05 .. AccI GT'mk_AC Cuts at: 0 620 1785 Size: 620 1165 Acc65I G'GTAC_C Cuts at: 0 329 1785 Size: 329 1456 AfeI AGC'GCT Cuts at: 0 1483 1785 Size: 1483 302 AflIII A'CryG_T Cuts at: 0 341 1785 Size: 341 1444 AleI CACnn'nnGTG Cuts at: 0 649 1785 Size: 649 1136 AlwNI CAG_nnn'CTG Cuts at: 0 786 1242 1785 Size: 786 456 543 Fragments arranged by size: 786 543 456 ApaI G_GGCC'C Cuts at: 0 1127 1343 1785 Size: 1127 216 442 Fragments arranged by size: 1127 442 216 ApaLI G'TGCA_C Cuts at: 0 1351 1785 Size: 1351 434 AvaI C'yCGr_G Cuts at: 0 889 1052 1096 1485 1785 Size: 889 163 44 389 300 Fragments arranged by size: 889 389 300 163 44 BamHI G'GATC_C Cuts at: 0 158 1785 Size: 158 1627 BanI G'GyrC_C Cuts at: 0 271 329 610 1381 1785 Size: 271 58 281 771 404 Fragments arranged by size: 771 404 281 271 58 BanII G_rGCy'C Cuts at: 0 389 1127 1343 1785 Size: 389 738 216 442 Fragments arranged by size: 738 442 389 216 BbeI G_GCGC'C Cuts at: 0 614 1785 Size: 614 1171 BbsI GAAGACnn'nnnn_ Cuts at: 0 592 1462 1785 Size: 592 870 323 Fragments arranged by size: 870 592 323 BbvCI CC'TCA_GC Cuts at: 0 415 1288 1785 Size: 415 873 497 Fragments arranged by size: 873 497 415 BciVI GTATCCnnnnn_n' Cuts at: 0 705 1785 Size: 705 1080 BglI GCCn_nnn'nGGC Cuts at: 0 1379 1785 Size: 1379 406 BglII A'GATC_T Cuts at: 0 1223 1785 Size: 1223 562 Bme1580I G_kGCm'C Cuts at: 0 1127 1343 1355 1785 Size: 1127 216 12 430 Fragments arranged by size: 1127 430 216 12 BmrI ACTGGGnnnn_n' Cuts at: 0 86 1262 1533 1785 Size: 86 1176 271 252 Fragments arranged by size: 1176 271 252 86 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 621 1434 1785 Size: 621 813 351 Fragments arranged by size: 813 621 351 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 446 1003 1588 1785 Size: 446 557 585 197 Fragments arranged by size: 585 557 446 197 Bpu10I CC'TnA_GC Cuts at: 0 415 484 1243 1288 1785 Size: 415 69 759 45 497 Fragments arranged by size: 759 497 415 69 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 271 1759 1785 Size: 37 234 1488 26 Fragments arranged by size: 1488 234 37 26 BsaI GGTCTCn'nnnn_ Cuts at: 0 14 37 538 1194 1202 1785 Size: 14 23 501 656 8 583 Fragments arranged by size: 656 583 501 23 14 8 BsaAI yAC'GTr Cuts at: 0 1527 1785 Size: 1527 258 BsaHI Gr'CG_yC Cuts at: 0 611 1785 Size: 611 1174 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 419 449 1495 1525 1588 1618 1672 1702 Size: 419 30 1046 30 63 30 54 30 Cuts at: 1702 1785 Size: 83 Fragments arranged by size: 1046 419 83 63 54 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 613 751 1735 1785 Size: 613 138 984 50 Fragments arranged by size: 984 613 138 50 BseYI C'CCAG_C Cuts at: 0 1626 1785 Size: 1626 159 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1516 1684 1785 Size: 1516 168 101 Fragments arranged by size: 1516 168 101 BsiEI CG_ry'CG Cuts at: 0 1374 1785 Size: 1374 411 BsiHKAI G_wGCw'C Cuts at: 0 449 1355 1785 Size: 449 906 430 Fragments arranged by size: 906 449 430 BsmI GAATG_Cn' Cuts at: 0 660 1644 1785 Size: 660 984 141 Fragments arranged by size: 984 660 141 BsmBI CGTCTCn'nnnn_ Cuts at: 0 355 1785 Size: 355 1430 Bsp1286I G_dGCh'C Cuts at: 0 389 449 1127 1343 1355 1785 Size: 389 60 678 216 12 430 Fragments arranged by size: 678 430 389 216 60 12 BspHI T'CATG_A Cuts at: 0 1043 1785 Size: 1043 742 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 632 1706 1785 Size: 632 1074 79 Fragments arranged by size: 1074 632 79 BsrBI CCG'CTC Cuts at: 0 483 1785 Size: 483 1302 BsrFI r'CCGG_y Cuts at: 0 1363 1785 Size: 1363 422 BssSI C'ACGA_G Cuts at: 0 645 1785 Size: 645 1140 BstEII G'GTnAC_C Cuts at: 0 144 489 1785 Size: 144 345 1296 Fragments arranged by size: 1296 345 144 BstXI CCAn_nnnn'nTGG Cuts at: 0 346 1431 1467 1785 Size: 346 1085 36 318 Fragments arranged by size: 1085 346 318 36 BstYI r'GATC_y Cuts at: 0 158 1223 1785 Size: 158 1065 562 Fragments arranged by size: 1065 562 158 Bsu36I CC'TnA_GG Cuts at: 0 606 1785 Size: 606 1179 BtgI C'CryG_G Cuts at: 0 69 333 989 1257 1785 Size: 69 264 656 268 528 Fragments arranged by size: 656 528 268 264 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 948 1518 1785 Size: 948 570 267 Fragments arranged by size: 948 570 267 BtsI GCAGTG_nn' Cuts at: 0 1353 1785 Size: 1353 432 DrdI GACnn_nn'nnGTC Cuts at: 0 1261 1785 Size: 1261 524 EaeI y'GGCC_r Cuts at: 0 336 799 1371 1529 1785 Size: 336 463 572 158 256 Fragments arranged by size: 572 463 336 256 158 EagI C'GGCC_G Cuts at: 0 1371 1785 Size: 1371 414 EarI CTCTTCn'nnn_ Cuts at: 0 1753 1785 Size: 1753 32 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 446 1003 1588 1785 Size: 446 557 585 197 Fragments arranged by size: 585 557 446 197 EcoNI CCTnn'n_nnAGG Cuts at: 0 52 1334 1785 Size: 52 1282 451 Fragments arranged by size: 1282 451 52 EcoO109I rG'GnC_Cy Cuts at: 0 937 1124 1263 1339 1428 1572 1785 Size: 937 187 139 76 89 144 213 Fragments arranged by size: 937 213 187 144 139 89 76 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 744 1785 Size: 744 1041 HaeII r_GCGC'y Cuts at: 0 614 1364 1485 1785 Size: 614 750 121 300 Fragments arranged by size: 750 614 300 121 KasI G'GCGC_C Cuts at: 0 610 1785 Size: 610 1175 KpnI G_GTAC'C Cuts at: 0 333 1785 Size: 333 1452 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 662 920 1580 1785 Size: 662 258 660 205 Fragments arranged by size: 662 660 258 205 MscI TGG'CCA Cuts at: 0 338 1531 1785 Size: 338 1193 254 Fragments arranged by size: 1193 338 254 MslI CAynn'nnrTG Cuts at: 0 649 1042 1348 1399 1405 1465 1525 1785 Size: 649 393 306 51 6 60 60 260 Fragments arranged by size: 649 393 306 260 60 60 51 6 MspA1I CmG'CkG Cuts at: 0 297 798 930 1197 1314 1740 1785 Size: 297 501 132 267 117 426 45 Fragments arranged by size: 501 426 297 267 132 117 45 NaeI GCC'GGC Cuts at: 0 1365 1785 Size: 1365 420 NarI GG'CG_CC Cuts at: 0 611 1785 Size: 611 1174 NcoI C'CATG_G Cuts at: 0 333 1257 1785 Size: 333 924 528 Fragments arranged by size: 924 528 333 NgoMIV G'CCGG_C Cuts at: 0 1363 1785 Size: 1363 422 NspI r_CATG'y Cuts at: 0 345 1110 1404 1785 Size: 345 765 294 381 Fragments arranged by size: 765 381 345 294 PciI A'CATG_T Cuts at: 0 341 1785 Size: 341 1444 PflMI CCAn_nnn'nTGG Cuts at: 0 786 1785 Size: 786 999 PfoI T'CCnGG_A Cuts at: 0 1295 1785 Size: 1295 490 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1752 1784 1785 Size: 1752 32 1 Fragments arranged by size: 1752 32 1 PpuMI rG'GwC_Cy Cuts at: 0 1263 1572 1785 Size: 1263 309 213 Fragments arranged by size: 1263 309 213 PspOMI G'GGCC_C Cuts at: 0 1123 1339 1785 Size: 1123 216 446 Fragments arranged by size: 1123 446 216 PstI C_TGCA'G Cuts at: 0 260 374 869 1360 1580 1785 Size: 260 114 495 491 220 205 Fragments arranged by size: 495 491 260 220 205 114 PvuII CAG'CTG Cuts at: 0 930 1785 Size: 930 855 SanDI GG'GwC_CC Cuts at: 0 1263 1785 Size: 1263 522 SapI GCTCTTCn'nnn_ Cuts at: 0 1753 1785 Size: 1753 32 SbfI CC_TGCA'GG Cuts at: 0 260 869 1785 Size: 260 609 916 Fragments arranged by size: 916 609 260 ScaI AGT'ACT Cuts at: 0 232 1785 Size: 232 1553 SexAI A'CCwGG_T Cuts at: 0 566 1785 Size: 566 1219 SfcI C'TryA_G Cuts at: 0 256 370 865 1356 1576 1785 Size: 256 114 495 491 220 209 Fragments arranged by size: 495 491 256 220 209 114 SfoI GGC'GCC Cuts at: 0 612 1785 Size: 612 1173 SmaI CCC'GGG Cuts at: 0 891 1785 Size: 891 894 SmlI C'TyrA_G Cuts at: 0 52 286 1774 1785 Size: 52 234 1488 11 Fragments arranged by size: 1488 234 52 11 SpeI A'CTAG_T Cuts at: 0 316 1785 Size: 316 1469 SphI G_CATG'C Cuts at: 0 1110 1785 Size: 1110 675 StuI AGG'CCT Cuts at: 0 604 1654 1785 Size: 604 1050 131 Fragments arranged by size: 1050 604 131 StyI C'CwwG_G Cuts at: 0 333 377 461 719 1257 1424 1649 1785 Size: 333 44 84 258 538 167 225 136 Fragments arranged by size: 538 333 258 225 167 136 84 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 508 523 1785 Size: 508 15 1262 Fragments arranged by size: 1262 508 15 TatI w'GTAC_w Cuts at: 0 230 1511 1785 Size: 230 1281 274 Fragments arranged by size: 1281 274 230 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 419 451 1785 Size: 419 32 1334 Fragments arranged by size: 1334 419 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 463 1056 1428 1785 Size: 463 593 372 357 Fragments arranged by size: 593 463 372 357 XmaI C'CCGG_G Cuts at: 0 889 1785 Size: 889 896 Enzymes that do cut: AccI Acc65I AfeI AflIII AleI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI HaeII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SanDI SapI SbfI ScaI SexAI SfcI SfoI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AclI AcuI AflII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BcgI BclI BfrBI BlpI BmgBI BmtI BsaBI BsaWI BsiWI BspEI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I ClaI CspCI DraI DraIII EciI EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PasI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SfiI SgfI SgrAI SnaBI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI