################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII AclI AcsI AflII AgeI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I AspI AvrII BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsgI BsiEI BsmBI BspDI BspHI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HpaI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccIII 2528 TCCGGA T^CCGGA AcyI 137 GGCGCC G[GA]^CG[CT]C AflIII 183 ACATGT A^C[GA][CT]GT AhdI 1202 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 1202 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1397 TGCGCA TGC^GCA BamHI 16 GGATCC G^GATCC BbeI 137 GGCGCC GGCGC^C BsaHI 137 GGCGCC G[GA]^CG[CT]C BsaWI 2528 TCCGGA [AT]^CCGG[AT] BseAI 2528 TCCGGA T^CCGGA BsiWI 1798 CGTACG C^GTACG BsmI 2239 GAATGC GAATGC(1/-1) Bsp1407I 2864 TGTACA T^GTACA BspEI 2528 TCCGGA T^CCGGA BspLU11I 183 ACATGT A^CATGT BsrGI 2864 TGTACA T^GTACA BssSI 1120 CACGAG CACGAG(-5/-1) BstAPI 2000 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Eam1105I 1202 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 1397 TGCGCA TGC^GCA Hin1I 137 GGCGCC G[GA]^CG[CT]C HincII 1210 GTCAAC GT[CT]^[GA]AC HindII 1210 GTCAAC GT[CT]^[GA]AC HindIII 666 AAGCTT A^AGCTT KasI 137 GGCGCC G^GCGCC KspI 2327 CCGCGG CCGC^GG MroI 2528 TCCGGA T^CCGGA MseI 1601 TTAA T^TAA NaeI 1459 GCCGGC GCC^GGC NarI 137 GGCGCC GG^CGCC NgoMIV 1459 GCCGGC G^CCGGC PaeR7I 1682 CTCGAG C^TCGAG SacII 2327 CCGCGG CCGC^GG SapI 2487 GAAGAGC GCTCTTC(1/4) SfoI 137 GGCGCC GGC^GCC SmaI 1673 CCCGGG CCC^GGG SspBI 2864 TGTACA T^GTACA SspI 1138 AATATT AAT^ATT StuI 2894 AGGCCT AGG^CCT Tru9I 1601 TTAA T^TAA Tsp509I 2687 AATT ^AATT XhoI 1682 CTCGAG C^TCGAG XmaCI 1673 CCCGGG C^CCGGG XmaI 1673 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Acc65I 79 GGTACC G^GTACC 1433 GGTACC AccI 1105 GTCTAC GT^[AC][GT]AC 1162 GTCTAC AocI 1966 CCTGAGG CC^T[ACGT]AGG 2658 CCTGAGG Aor51HI 2183 AGCGCT AGC^GCT 3122 AGCGCT Asp718I 79 GGTACC G^GTACC 1433 GGTACC AspHI 1069 GAGCAC G[AT]GC[AT]^C 2755 GAGCAC BlpI 48 GCTCAGC GC^T[ACGT]AGC 2602 GCTGAGC Bpu1102I 48 GCTCAGC GC^T[ACGT]AGC 2602 GCTGAGC BsaBI 1347 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1920 GATGCACATC BsiHKAI 1069 GAGCAC G[AT]GC[AT]^C 2755 GAGCAC BsrBI 226 GAGCGG CCGCTC(-3/-3) 544 GAGCGG Bsu36I 1966 CCTGAGG CC^T[ACGT]AGG 2658 CCTGAGG CelII 48 GCTCAGC GC^T[ACGT]AGC 2602 GCTGAGC EarI 2409 GAAGAG CTCTTC(1/4) 2487 GAAGAG Eco47III 2183 AGCGCT AGC^GCT 3122 AGCGCT Eco81I 1966 CCTGAGG CC^T[ACGT]AGG 2658 CCTGAGG KpnI 79 GGTACC GGTAC^C 1433 GGTACC Ksp632I 2409 GAAGAG CTCTTC(1/4) 2487 GAAGAG MamI 1347 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1920 GATGCACATC NcoI 1095 CCATGG C^CATGG 3018 CCATGG NspI 183 ACATGT [GA]CATG^[CT] 971 ACATGC PshAI 429 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 864 GACCCAGGTC ScaI 2876 AGTACT AGT^ACT 3042 AGTACT TfiI 1737 GATTC G^A[AT]TC 1866 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII