################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccIII AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BfrI BglI BlnI BsaAI BsaBI BseAI BseRI BsiEI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PvuI RcaI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 307 GTCTAC GT^[AC][GT]AC AccII 203 CGCG CG^CG AcyI 1438 GGCGTC G[GA]^CG[CT]C AflIII 2477 ACGTGT A^C[GA][CT]GT ApaI 451 GGGCCC GGGCC^C AseI 1759 ATTAAT AT^TAAT AsnI 1759 ATTAAT AT^TAAT AspHI 2076 GAGCAC G[AT]GC[AT]^C AvaI 1412 CCCGAG C^[CT]CG[GA]G BalI 2312 TGGCCA TGG^CCA BanII 451 GGGCCC G[GA]GC[CT]^C BciVI 1584 GGATAC GTATCC(6/5) BclI 1493 TGATCA T^GATCA BglII 684 AGATCT A^GATCT BlpI 858 GCTTAGC GC^T[ACGT]AGC BpmI 623 CTCCAG CTGGAG(16/14) Bpu1102I 858 GCTTAGC GC^T[ACGT]AGC BsaHI 1438 GGCGTC G[GA]^CG[CT]C BsaWI 1432 ACCGGA [AT]^CCGG[AT] BsgI 1832 GTGCAG GTGCAG(16/14) BsiHKAI 2076 GAGCAC G[AT]GC[AT]^C BsoBI 1412 CCCGAG C^[CT]CG[GA]G BsrBI 2318 CCGCTC CCGCTC(-3/-3) BssSI 1557 CACGAG CACGAG(-5/-1) BstUI 203 CGCG CG^CG BstXI 138 CCATAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 684 AGATCT [GA]^GATC[CT] CelII 858 GCTTAGC GC^T[ACGT]AGC DraII 451 GGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 2312 TGGCCA [CT]^GGCC[GA] EcoNI 455 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 451 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoRV 1174 GATATC GAT^ATC FbaI 1493 TGATCA T^GATCA Hin1I 1438 GGCGTC G[GA]^CG[CT]C MflI 684 AGATCT [GA]^GATC[CT] MluNI 2312 TGGCCA TGG^CCA MscI 2312 TGGCCA TGG^CCA MspA1I 973 CAGCTG C[AC]G^C[GT]G MvnI 203 CGCG CG^CG NcoI 773 CCATGG C^CATGG NspI 1395 GCATGC [GA]CATG^[CT] PshBI 1759 ATTAAT AT^TAAT PvuII 973 CAGCTG CAG^CTG SapI 831 GAAGAGC GCTCTTC(1/4) ScaI 2450 AGTACT AGT^ACT SexAI 542 ACCAGGT A^CC[AT]GGT SpeI 171 ACTAGT A^CTAGT SphI 1395 GCATGC GCATG^C Tsp45I 1682 GTGAC ^GT[GC]AC XhoII 684 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AclI 1030 AACGTT AA^CGTT 1039 AACGTT AlwI 557 GGATC GGATC(4/5) 2267 GGATC AlwNI 278 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG 1680 CAGTGACTG AocI 85 CCTAAGG CC^T[ACGT]AGG 459 CCTGAGG Asp700I 1728 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2462 GAATGGATTC AvaII 148 GGACC G^G[AT]CC 1068 GGTCC BcnI 554 CCCGG CC^[GC]GG 1575 CCGGG BmyI 451 GGGCCC G[AGT]GC[ACT]^C 2076 GAGCAC BsmI 158 GCATTC GAATGC(1/-1) 1222 GCATTC Bsp1286I 451 GGGCCC G[AGT]GC[ACT]^C 2076 GAGCAC Bsu36I 85 CCTAAGG CC^T[ACGT]AGG 459 CCTGAGG DsaI 773 CCATGG C^C[GA][CT]GG 1010 CCGTGG Eco81I 85 CCTAAGG CC^T[ACGT]AGG 459 CCTGAGG EcoRI 719 GAATTC G^AATTC 1158 GAATTC EcoT14I 665 CCAAGG C^C[AT][AT]GG 773 CCATGG FauI 1924 CCCGC CCCGC(4/6) 41 GCGGG MslI 1295 CATACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2548 CATGTATATG NciI 554 CCCGG CC^[GC]GG 1575 CCGGG NdeI 248 CATATG CA^TATG 1952 CATATG Psp1406I 1030 AACGTT AA^CGTT 1039 AACGTT PstI 1723 CTGCAG CTGCA^G 2260 CTGCAG StyI 665 CCAAGG C^C[AT][AT]GG 773 CCATGG XbaI 1959 TCTAGA T^CTAGA 2322 TCTAGA XcmI 797 CCAAAGAGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2504 CCATGAAAAATATGG XmnI 1728 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2462 GAATGGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbsI BbvI BfaI BpuAI BsaI BsaJI BsiYI BslI BsmAI BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I