(Circular) (Six-base) MAPSORT of: pFN21AE2703.seq Check: 8284 from: 1 to: 7300 pFN21AE2703 7300 bp With 182 enzymes: SgfI * September 21, 2017 12:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3735 3735 Size: 7300 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5110 278 Size: 53 83 186 936 3574 2468 Fragments arranged by size: 3574 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2313 4643 1937 Size: 376 2330 4594 Fragments arranged by size: 4594 2330 376 Acc65I G'GTAC_C Cuts at: 4621 4621 Size: 7300 AclI AA'CG_TT Cuts at: 1563 3036 3045 5427 5800 1563 Size: 1473 9 2382 373 3063 Fragments arranged by size: 3063 2382 1473 373 9 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3827 3842 5363 6747 7015 1632 Size: 2195 15 1521 1384 268 1917 Fragments arranged by size: 2195 1917 1521 1384 268 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7103 AflIII A'CryG_T Cuts at: 1203 4482 4597 6199 7176 1203 Size: 3279 115 1602 977 1327 Fragments arranged by size: 3279 1602 1327 977 115 AgeI A'CCGG_T Cuts at: 6131 6131 Size: 7300 AhdI GACnn_n'nnGTC Cuts at: 6029 6029 Size: 7300 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7300 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7300 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2666 2698 2666 Size: 32 7268 AlwNI CAG_nnn'CTG Cuts at: 2288 3690 5043 6615 2288 Size: 1402 1353 1572 2973 Fragments arranged by size: 2973 1572 1402 1353 ApaI G_GGCC'C Cuts at: 2460 2460 Size: 7300 ApaLI G'TGCA_C Cuts at: 5357 6513 5357 Size: 1156 6144 ApoI r'AATT_y Cuts at: 1493 2237 2724 3163 3271 3997 4378 4609 4835 Size: 744 487 439 108 726 381 231 226 Cuts at: 4835 4987 7096 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 744 726 487 439 381 231 226 152 108 AseI AT'TA_AT Cuts at: 160 1032 3765 5854 160 Size: 872 2733 2089 1606 Fragments arranged by size: 2733 2089 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7300 AvaI C'yCGr_G Cuts at: 1870 1943 3417 4625 1870 Size: 73 1474 1208 4545 Fragments arranged by size: 4545 1474 1208 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4323 4356 4323 Size: 33 7267 BamHI G'GATC_C Cuts at: 4630 4997 4630 Size: 367 6933 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4621 6076 618 Size: 325 217 627 2834 1455 1842 Fragments arranged by size: 2834 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2460 4619 1391 Size: 1069 2159 4072 Fragments arranged by size: 4072 2159 1069 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3194 3439 3618 928 Size: 971 1295 245 179 4610 Fragments arranged by size: 4610 1295 971 245 179 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5491 5525 1903 Size: 34 3554 34 3678 Fragments arranged by size: 3678 3554 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3583 5192 6402 3583 Size: 1609 1210 4481 Fragments arranged by size: 4481 1609 1210 BclI T'GATC_A Cuts at: 1548 3498 1548 Size: 1950 5350 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5911 136 Size: 107 122 71 631 605 4239 1525 Fragments arranged by size: 4239 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2689 7295 2689 Size: 4606 2694 BlpI GC'TnA_GC Cuts at: 2864 4717 2864 Size: 1853 5447 Bme1580I G_kGCm'C Cuts at: 1790 2460 5361 6517 1790 Size: 670 2901 1156 2573 Fragments arranged by size: 2901 2573 1156 670 BmgBI CAC'GTC Cuts at: 1318 4483 1318 Size: 3165 4135 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2650 3625 5031 Size: 416 53 83 399 24 1233 975 1406 Cuts at: 5031 5989 442 Size: 958 1753 Fragments arranged by size: 1753 1406 1233 975 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7300 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2613 5960 1239 Size: 1374 3347 2579 Fragments arranged by size: 3347 2579 1374 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5425 6290 6588 5425 Size: 865 298 6137 Fragments arranged by size: 6137 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2123 2734 2792 5963 882 Size: 617 624 611 58 3171 2219 Fragments arranged by size: 3171 2219 624 617 611 58 BsaAI yAC'GTr Cuts at: 493 1204 5091 493 Size: 711 3887 2702 Fragments arranged by size: 3887 2702 711 BsaBI GATnn'nnATC Cuts at: 7294 7294 Size: 7300 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3444 5107 5489 6124 Size: 53 83 186 936 1911 1663 382 635 Cuts at: 6124 275 Size: 1451 Fragments arranged by size: 1911 1663 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3437 5733 6131 6405 6552 6963 3437 Size: 2296 398 274 147 411 3774 Fragments arranged by size: 3774 2296 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2071 2101 4469 4499 1178 Size: 30 165 30 668 30 2368 30 3979 Fragments arranged by size: 3979 2368 668 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7300 BseYI C'CCAG_C Cuts at: 2035 3310 6503 6999 2035 Size: 1275 3193 496 2336 Fragments arranged by size: 3193 2336 1275 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3858 1163 Size: 573 2122 4605 Fragments arranged by size: 4605 2122 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4750 5511 6539 1540 Size: 275 186 2749 761 1028 2301 Fragments arranged by size: 2749 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4085 5361 5446 6517 4085 Size: 1276 85 1071 4868 Fragments arranged by size: 4868 1276 1071 85 BsmI GAATG_Cn' Cuts at: 2163 3227 4811 4904 2163 Size: 1064 1584 93 4559 Fragments arranged by size: 4559 1584 1064 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2460 4085 4619 5361 5446 6517 1391 Size: 399 670 1625 534 742 85 1071 2174 Fragments arranged by size: 2174 1625 1071 742 670 534 399 85 BspEI T'CCGG_A Cuts at: 6963 6963 Size: 7300 BspHI T'CATG_A Cuts at: 5189 6919 5189 Size: 1730 5570 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2957 3134 3735 4655 844 Size: 2113 177 601 920 3489 Fragments arranged by size: 3489 2113 920 601 177 BsrBI CCG'CTC Cuts at: 1394 1823 4325 5187 1394 Size: 429 2502 862 3507 Fragments arranged by size: 3507 2502 862 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5795 5969 65 Size: 1183 4547 174 1396 Fragments arranged by size: 4547 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5944 6131 1700 Size: 110 4134 187 2869 Fragments arranged by size: 4134 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7300 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7300 BssSI C'ACGA_G Cuts at: 3562 5360 6372 3562 Size: 1798 1012 4490 Fragments arranged by size: 4490 1798 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7300 BstBI TT'CG_AA Cuts at: 6104 6104 Size: 7300 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7300 BstXI CCAn_nnnn'nTGG Cuts at: 2150 2150 Size: 7300 BstYI r'GATC_y Cuts at: 1972 2689 4630 4997 5382 5399 6851 6937 6949 Size: 717 1941 367 385 17 1452 86 12 Cuts at: 6949 7295 1972 Size: 346 1977 Fragments arranged by size: 1977 1941 1452 717 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2091 2465 2091 Size: 374 6926 BtgI C'CryG_G Cuts at: 513 1065 2004 2778 3015 513 Size: 552 939 774 237 4798 Fragments arranged by size: 4798 939 774 552 237 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6561 Fragments arranged by size: 6561 683 56 BtsI GCAGTG_nn' Cuts at: 3796 4274 4816 5610 5630 3796 Size: 478 542 794 20 5466 Fragments arranged by size: 5466 794 542 478 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7300 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2404 2439 529 Size: 35 1840 35 5390 Fragments arranged by size: 5390 1840 35 35 DraI TTT'AAA Cuts at: 4604 4604 Size: 7300 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6307 809 Size: 507 4991 1802 Fragments arranged by size: 4991 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4317 4747 5636 Size: 54 1425 50 275 18 2487 430 889 Cuts at: 5636 7265 8 Size: 1629 43 Fragments arranged by size: 2487 1629 1425 889 430 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4747 1537 Size: 275 2935 4090 Fragments arranged by size: 4090 2935 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2831 3282 3726 5230 1353 Size: 1478 451 444 1504 3423 Fragments arranged by size: 3423 1504 1478 451 444 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2736 5887 6261 6407 387 Size: 1064 138 1147 3151 374 146 1280 Fragments arranged by size: 3151 1280 1147 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2613 3827 3842 5363 5960 6747 7015 Size: 393 981 1214 15 1521 597 787 268 Cuts at: 7015 1239 Size: 1524 Fragments arranged by size: 1524 1521 1214 981 787 597 393 268 15 EcoNI CCTnn'n_nnAGG Cuts at: 2464 2464 Size: 7300 EcoO109I rG'GnC_Cy Cuts at: 2457 2457 Size: 7300 EcoRI G'AATT_C Cuts at: 2724 3163 4609 4987 2724 Size: 439 1446 378 5037 Fragments arranged by size: 5037 1446 439 378 EcoRV GAT'ATC Cuts at: 3181 3181 Size: 7300 FspI TGC'GCA Cuts at: 5806 5806 Size: 7300 HaeII r_GCGC'y Cuts at: 1678 6447 1678 Size: 4769 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2733 2759 2765 2791 3206 Size: 32 135 32 1161 26 6 26 415 Cuts at: 3206 3238 3411 3443 5941 5973 6015 6047 1373 Size: 32 173 32 2498 32 42 32 2626 Fragments arranged by size: 2626 2498 1161 415 173 135 42 32 32 32 32 32 32 26 26 6 HincII GTy'rAC Cuts at: 669 1657 1938 4644 4890 669 Size: 988 281 2706 246 3079 Fragments arranged by size: 3079 2706 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7300 HpaI GTT'AAC Cuts at: 4890 4890 Size: 7300 KpnI G_GTAC'C Cuts at: 4625 4625 Size: 7300 MfeI C'AATT_G Cuts at: 4899 4899 Size: 7300 MluI A'CGCG_T Cuts at: 7176 7176 Size: 7300 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2555 2793 3100 3439 3445 3456 4113 6414 Size: 1250 238 307 339 6 11 657 2301 Cuts at: 6414 6598 1305 Size: 184 2007 Fragments arranged by size: 2301 2007 1250 657 339 307 238 184 11 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 4319 10 Size: 54 1425 343 2487 2991 Fragments arranged by size: 2991 2487 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3304 4557 5258 5617 5776 518 Size: 648 2138 1253 701 359 159 2042 Fragments arranged by size: 2138 2042 1253 701 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2980 4716 5393 6541 6786 1606 Size: 1374 1736 677 1148 245 2120 Fragments arranged by size: 2120 1736 1374 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2778 513 Size: 552 939 774 5035 Fragments arranged by size: 5035 939 774 552 NdeI CA'TA_TG Cuts at: 387 2254 3958 387 Size: 1867 1704 3729 Fragments arranged by size: 3729 1867 1704 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7300 NotI GC'GGCC_GC Cuts at: 4747 4747 Size: 7300 NspI r_CATG'y Cuts at: 1132 1229 3404 4601 4658 6203 1132 Size: 97 2175 1197 57 1545 2229 Fragments arranged by size: 2229 2175 1545 1197 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7300 PciI A'CATG_T Cuts at: 4597 6199 4597 Size: 1602 5698 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7300 PmeI GTTT'AAAC Cuts at: 4604 4604 Size: 7300 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5369 5401 6882 6914 5369 Size: 32 1481 32 5755 Fragments arranged by size: 5755 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7300 PsiI TTA'TAA Cuts at: 4870 4870 Size: 7300 PspOMI G'GGCC_C Cuts at: 2456 2456 Size: 7300 PstI C_TGCA'G Cuts at: 830 3732 4269 4652 830 Size: 2902 537 383 3478 Fragments arranged by size: 3478 2902 537 383 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7300 PvuII CAG'CTG Cuts at: 2980 2980 Size: 7300 SalI G'TCGA_C Cuts at: 1936 4642 1936 Size: 2706 4594 SapI GCTCTTCn'nnn_ Cuts at: 2831 2831 Size: 7300 SbfI CC_TGCA'GG Cuts at: 4652 4652 Size: 7300 ScaI AGT'ACT Cuts at: 4457 5548 7260 4457 Size: 1091 1712 4497 Fragments arranged by size: 4497 1712 1091 SexAI A'CCwGG_T Cuts at: 2547 2547 Size: 7300 SfcI C'TryA_G Cuts at: 826 1045 3728 4065 4265 4648 5783 6464 6655 Size: 219 2683 337 200 383 1135 681 191 Cuts at: 6655 826 Size: 1471 Fragments arranged by size: 2683 1471 1135 681 383 337 219 200 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7300 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7300 SmaI CCC'GGG Cuts at: 1872 4627 1872 Size: 2755 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5404 6305 6567 820 Size: 197 926 3461 901 262 1553 Fragments arranged by size: 3461 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5091 493 Size: 4598 2702 SpeI A'CTAG_T Cuts at: 152 2176 152 Size: 2024 5276 SphI G_CATG'C Cuts at: 1132 3404 4658 1132 Size: 2272 1254 3774 Fragments arranged by size: 3774 2272 1254 SspI AAT'ATT Cuts at: 5 52 5224 5 Size: 47 5172 2081 Fragments arranged by size: 5172 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7300 StyI C'CwwG_G Cuts at: 513 1065 2004 2670 2778 4739 513 Size: 552 939 666 108 1961 3074 Fragments arranged by size: 3074 1961 939 666 552 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2748 3302 3824 5327 5344 5497 5682 Size: 1255 1168 554 522 1503 17 153 185 Cuts at: 5682 6127 325 Size: 445 1498 Fragments arranged by size: 1503 1498 1255 1168 554 522 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2658 2855 3115 Size: 274 80 33 51 1443 681 197 260 Cuts at: 3115 4455 5546 7258 96 Size: 1340 1091 1712 138 Fragments arranged by size: 1712 1443 1340 1091 681 274 260 197 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2071 2103 7260 7292 2071 Size: 32 5157 32 2079 Fragments arranged by size: 5157 2079 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7300 XbaI T'CTAG_A Cuts at: 3964 4327 4636 3964 Size: 363 309 6628 Fragments arranged by size: 6628 363 309 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2809 4516 2809 Size: 1707 5593 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7300 XmaI C'CCGG_G Cuts at: 1870 4625 1870 Size: 2755 4545 XmnI GAAnn'nnTTC Cuts at: 3737 4471 5429 3737 Size: 734 958 5608 Fragments arranged by size: 5608 958 734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5108 276 Size: 53 83 186 936 3574 2468 Fragments arranged by size: 3574 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI