(Linear) (Six-base) MAPSORT of: KIEE2703.seq Check: 1664 from: 1 to: 2595 KIEE2703.orf With 182 enzymes: SgfI * September 21, 2017 11:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1730 2595 Size: 1730 865 AccI GT'mk_AC Cuts at: 0 308 2595 Size: 308 2287 AclI AA'CG_TT Cuts at: 0 1031 1040 2595 Size: 1031 9 1555 Fragments arranged by size: 1555 1031 9 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1822 1837 2595 Size: 1822 15 758 Fragments arranged by size: 1822 758 15 AflIII A'CryG_T Cuts at: 0 2477 2595 Size: 2477 118 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 661 693 2595 Size: 661 32 1902 Fragments arranged by size: 1902 661 32 AlwNI CAG_nnn'CTG Cuts at: 0 283 1685 2595 Size: 283 1402 910 Fragments arranged by size: 1402 910 283 ApaI G_GGCC'C Cuts at: 0 455 2595 Size: 455 2140 ApoI r'AATT_y Cuts at: 0 232 719 1158 1266 1992 2373 2595 Size: 232 487 439 108 726 381 222 Fragments arranged by size: 726 487 439 381 232 222 108 AseI AT'TA_AT Cuts at: 0 1760 2595 Size: 1760 835 AvaI C'yCGr_G Cuts at: 0 1412 2595 Size: 1412 1183 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2318 2351 2595 Size: 2318 33 244 Fragments arranged by size: 2318 244 33 BanII G_rGCy'C Cuts at: 0 455 2595 Size: 455 2140 BbsI GAAGACnn'nnnn_ Cuts at: 0 1189 1434 1613 2595 Size: 1189 245 179 982 Fragments arranged by size: 1189 982 245 179 BciVI GTATCCnnnnn_n' Cuts at: 0 1578 2595 Size: 1578 1017 BclI T'GATC_A Cuts at: 0 1493 2595 Size: 1493 1102 BglII A'GATC_T Cuts at: 0 684 2595 Size: 684 1911 BlpI GC'TnA_GC Cuts at: 0 859 2595 Size: 859 1736 Bme1580I G_kGCm'C Cuts at: 0 455 2595 Size: 455 2140 BmgBI CAC'GTC Cuts at: 0 2478 2595 Size: 2478 117 BmrI ACTGGGnnnn_n' Cuts at: 0 645 1620 2595 Size: 645 975 975 Fragments arranged by size: 975 975 645 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 608 2595 Size: 608 1987 BsaI GGTCTCn'nnnn_ Cuts at: 0 118 729 787 2595 Size: 118 611 58 1808 Fragments arranged by size: 1808 611 118 58 BsaHI Gr'CG_yC Cuts at: 0 1439 2595 Size: 1439 1156 BsaWI w'CCGG_w Cuts at: 0 1432 2595 Size: 1432 1163 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 66 96 2464 2494 2595 Size: 66 30 2368 30 101 Fragments arranged by size: 2368 101 66 30 30 BseYI C'CCAG_C Cuts at: 0 30 1305 2595 Size: 30 1275 1290 Fragments arranged by size: 1290 1275 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1853 2595 Size: 1853 742 BsiHKAI G_wGCw'C Cuts at: 0 2080 2595 Size: 2080 515 BsmI GAATG_Cn' Cuts at: 0 158 1222 2595 Size: 158 1064 1373 Fragments arranged by size: 1373 1064 158 Bsp1286I G_dGCh'C Cuts at: 0 455 2080 2595 Size: 455 1625 515 Fragments arranged by size: 1625 515 455 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 952 1129 1730 2595 Size: 952 177 601 865 Fragments arranged by size: 952 865 601 177 BsrBI CCG'CTC Cuts at: 0 2320 2595 Size: 2320 275 BssSI C'ACGA_G Cuts at: 0 1557 2595 Size: 1557 1038 BstXI CCAn_nnnn'nTGG Cuts at: 0 145 2595 Size: 145 2450 BstYI r'GATC_y Cuts at: 0 684 2595 Size: 684 1911 Bsu36I CC'TnA_GG Cuts at: 0 86 460 2595 Size: 86 374 2135 Fragments arranged by size: 2135 374 86 BtgI C'CryG_G Cuts at: 0 773 1010 2595 Size: 773 237 1585 Fragments arranged by size: 1585 773 237 BtsI GCAGTG_nn' Cuts at: 0 1791 2269 2595 Size: 1791 478 326 Fragments arranged by size: 1791 478 326 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 399 434 2595 Size: 399 35 2161 Fragments arranged by size: 2161 399 35 EaeI y'GGCC_r Cuts at: 0 2312 2595 Size: 2312 283 EarI CTCTTCn'nnn_ Cuts at: 0 826 1277 1721 2595 Size: 826 451 444 874 Fragments arranged by size: 874 826 451 444 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 731 2595 Size: 731 1864 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 608 1822 1837 2595 Size: 608 1214 15 758 Fragments arranged by size: 1214 758 608 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 459 2595 Size: 459 2136 EcoO109I rG'GnC_Cy Cuts at: 0 452 2595 Size: 452 2143 EcoRI G'AATT_C Cuts at: 0 719 1158 2595 Size: 719 439 1437 Fragments arranged by size: 1437 719 439 EcoRV GAT'ATC Cuts at: 0 1176 2595 Size: 1176 1419 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 728 754 760 786 1201 1233 1406 1438 Size: 728 26 6 26 415 32 173 32 Cuts at: 1438 2595 Size: 1157 Fragments arranged by size: 1157 728 415 173 32 32 26 26 6 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 550 788 1095 1434 1440 1451 2108 2595 Size: 550 238 307 339 6 11 657 487 Fragments arranged by size: 657 550 487 339 307 238 11 6 MscI TGG'CCA Cuts at: 0 2314 2595 Size: 2314 281 MslI CAynn'nnrTG Cuts at: 0 1299 2552 2595 Size: 1299 1253 43 Fragments arranged by size: 1299 1253 43 MspA1I CmG'CkG Cuts at: 0 975 2595 Size: 975 1620 NcoI C'CATG_G Cuts at: 0 773 2595 Size: 773 1822 NdeI CA'TA_TG Cuts at: 0 249 1953 2595 Size: 249 1704 642 Fragments arranged by size: 1704 642 249 NspI r_CATG'y Cuts at: 0 1399 2595 Size: 1399 1196 PspOMI G'GGCC_C Cuts at: 0 451 2595 Size: 451 2144 PstI C_TGCA'G Cuts at: 0 1727 2264 2595 Size: 1727 537 331 Fragments arranged by size: 1727 537 331 PvuII CAG'CTG Cuts at: 0 975 2595 Size: 975 1620 SapI GCTCTTCn'nnn_ Cuts at: 0 826 2595 Size: 826 1769 ScaI AGT'ACT Cuts at: 0 2452 2595 Size: 2452 143 SexAI A'CCwGG_T Cuts at: 0 542 2595 Size: 542 2053 SfcI C'TryA_G Cuts at: 0 1723 2060 2260 2595 Size: 1723 337 200 335 Fragments arranged by size: 1723 337 335 200 SpeI A'CTAG_T Cuts at: 0 171 2595 Size: 171 2424 SphI G_CATG'C Cuts at: 0 1399 2595 Size: 1399 1196 StyI C'CwwG_G Cuts at: 0 665 773 2595 Size: 665 108 1822 Fragments arranged by size: 1822 665 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 743 1297 1819 2595 Size: 743 554 522 776 Fragments arranged by size: 776 743 554 522 TatI w'GTAC_w Cuts at: 0 653 850 1110 2450 2595 Size: 653 197 260 1340 145 Fragments arranged by size: 1340 653 260 197 145 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 66 98 2595 Size: 66 32 2497 Fragments arranged by size: 2497 66 32 XbaI T'CTAG_A Cuts at: 0 1959 2322 2595 Size: 1959 363 273 Fragments arranged by size: 1959 363 273 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 804 2511 2595 Size: 804 1707 84 Fragments arranged by size: 1707 804 84 XmnI GAAnn'nnTTC Cuts at: 0 1732 2466 2595 Size: 1732 734 129 Fragments arranged by size: 1732 734 129 Enzymes that do cut: AarI AccI AclI AcuI AflIII AloI AlwNI ApaI ApoI AseI AvaI BaeI BanII BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BssSI BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I MmeI MscI MslI MspA1I NcoI NdeI NspI PspOMI PstI PvuII SapI ScaI SexAI SfcI SpeI SphI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AatII Acc65I AfeI AflII AgeI AhdI AleI AlfI ApaLI AscI AsiSI AvrII BamHI BanI BbeI BbvCI BcgI BfrBI BglI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BseRI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EagI EcoICRI FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SrfI SspI StuI SwaI Tth111I XhoI XmaI ZraI