(Linear) (Six-base) MAPSORT of: KIEE2779.seq Check: 6866 from: 1 to: 2427 KIEE2779.orf With 182 enzymes: SgfI * July 3, 2020 20:21 .. Acc65I G'GTAC_C Cuts at: 0 700 2427 Size: 700 1727 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 2102 2266 2326 2427 Size: 153 1949 164 60 101 Fragments arranged by size: 1949 164 153 101 60 AfeI AGC'GCT Cuts at: 0 343 1522 2427 Size: 343 1179 905 Fragments arranged by size: 1179 905 343 AleI CACnn'nnGTG Cuts at: 0 2356 2427 Size: 2356 71 AlwNI CAG_nnn'CTG Cuts at: 0 2191 2427 Size: 2191 236 ApoI r'AATT_y Cuts at: 0 1407 1649 2047 2284 2427 Size: 1407 242 398 237 143 Fragments arranged by size: 1407 398 242 237 143 AseI AT'TA_AT Cuts at: 0 509 1279 1939 2427 Size: 509 770 660 488 Fragments arranged by size: 770 660 509 488 BamHI G'GATC_C Cuts at: 0 388 2427 Size: 388 2039 BanI G'GyrC_C Cuts at: 0 151 700 1218 2427 Size: 151 549 518 1209 Fragments arranged by size: 1209 549 518 151 BanII G_rGCy'C Cuts at: 0 1571 2427 Size: 1571 856 BbsI GAAGACnn'nnnn_ Cuts at: 0 1368 2406 2427 Size: 1368 1038 21 Fragments arranged by size: 1368 1038 21 BbvCI CC'TCA_GC Cuts at: 0 1537 2427 Size: 1537 890 BclI T'GATC_A Cuts at: 0 1083 1911 2427 Size: 1083 828 516 Fragments arranged by size: 1083 828 516 BfrBI ATG'CAT Cuts at: 0 1234 1415 2427 Size: 1234 181 1012 Fragments arranged by size: 1234 1012 181 BglII A'GATC_T Cuts at: 0 80 2427 Size: 80 2347 Bme1580I G_kGCm'C Cuts at: 0 135 154 2427 Size: 135 19 2273 Fragments arranged by size: 2273 135 19 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 910 1604 2240 2427 Size: 910 694 636 187 Fragments arranged by size: 910 694 636 187 Bpu10I CC'TnA_GC Cuts at: 0 1537 2427 Size: 1537 890 BsaHI Gr'CG_yC Cuts at: 0 353 2427 Size: 353 2074 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 866 896 941 971 1431 1461 2126 2156 Size: 866 30 45 30 460 30 665 30 Cuts at: 2156 2427 Size: 271 Fragments arranged by size: 866 665 460 271 45 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 385 965 1870 2427 Size: 385 580 905 557 Fragments arranged by size: 905 580 557 385 BseYI C'CCAG_C Cuts at: 0 276 2427 Size: 276 2151 BsiHKAI G_wGCw'C Cuts at: 0 627 1571 2427 Size: 627 944 856 Fragments arranged by size: 944 856 627 BsmI GAATG_Cn' Cuts at: 0 492 2427 Size: 492 1935 BsmBI CGTCTCn'nnnn_ Cuts at: 0 788 958 2427 Size: 788 170 1469 Fragments arranged by size: 1469 788 170 Bsp1286I G_dGCh'C Cuts at: 0 135 154 627 1571 2427 Size: 135 19 473 944 856 Fragments arranged by size: 944 856 473 135 19 BspHI T'CATG_A Cuts at: 0 1841 2427 Size: 1841 586 BssSI C'ACGA_G Cuts at: 0 848 2165 2427 Size: 848 1317 262 Fragments arranged by size: 1317 848 262 BstEII G'GTnAC_C Cuts at: 0 2268 2277 2427 Size: 2268 9 150 Fragments arranged by size: 2268 150 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 304 2427 Size: 304 2123 BstYI r'GATC_y Cuts at: 0 80 388 1155 2392 2427 Size: 80 308 767 1237 35 Fragments arranged by size: 1237 767 308 80 35 BtgI C'CryG_G Cuts at: 0 636 2091 2427 Size: 636 1455 336 Fragments arranged by size: 1455 636 336 DraI TTT'AAA Cuts at: 0 1754 2427 Size: 1754 673 DraIII CAC_nnn'GTG Cuts at: 0 853 2427 Size: 853 1574 EarI CTCTTCn'nnn_ Cuts at: 0 786 1679 2258 2427 Size: 786 893 579 169 Fragments arranged by size: 893 786 579 169 EcoICRI GAG'CTC Cuts at: 0 1569 2427 Size: 1569 858 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 910 1604 2102 2240 2266 2326 2427 Size: 153 757 694 498 138 26 60 101 Fragments arranged by size: 757 694 498 153 138 101 60 26 EcoO109I rG'GnC_Cy Cuts at: 0 783 1089 2188 2427 Size: 783 306 1099 239 Fragments arranged by size: 1099 783 306 239 EcoRI G'AATT_C Cuts at: 0 2047 2427 Size: 2047 380 EcoRV GAT'ATC Cuts at: 0 444 2427 Size: 444 1983 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 183 408 754 1333 2427 Size: 183 225 346 579 1094 Fragments arranged by size: 1094 579 346 225 183 HaeII r_GCGC'y Cuts at: 0 345 1269 1524 2427 Size: 345 924 255 903 Fragments arranged by size: 924 903 345 255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 72 104 727 759 941 973 2427 Size: 72 32 623 32 182 32 1454 Fragments arranged by size: 1454 623 182 72 32 32 32 HincII GTy'rAC Cuts at: 0 48 524 2427 Size: 48 476 1903 Fragments arranged by size: 1903 476 48 HpaI GTT'AAC Cuts at: 0 48 2427 Size: 48 2379 KpnI G_GTAC'C Cuts at: 0 704 2427 Size: 704 1723 MfeI C'AATT_G Cuts at: 0 983 2427 Size: 983 1444 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1884 2427 Size: 1884 543 MslI CAynn'nnrTG Cuts at: 0 302 2356 2427 Size: 302 2054 71 Fragments arranged by size: 2054 302 71 MspA1I CmG'CkG Cuts at: 0 757 1393 2427 Size: 757 636 1034 Fragments arranged by size: 1034 757 636 NcoI C'CATG_G Cuts at: 0 636 2091 2427 Size: 636 1455 336 Fragments arranged by size: 1455 636 336 NsiI A_TGCA'T Cuts at: 0 1236 1417 2427 Size: 1236 181 1010 Fragments arranged by size: 1236 1010 181 PpuMI rG'GwC_Cy Cuts at: 0 1089 2188 2427 Size: 1089 1099 239 Fragments arranged by size: 1099 1089 239 PstI C_TGCA'G Cuts at: 0 1289 1557 2427 Size: 1289 268 870 Fragments arranged by size: 1289 870 268 PvuII CAG'CTG Cuts at: 0 757 1393 2427 Size: 757 636 1034 Fragments arranged by size: 1034 757 636 SacI G_AGCT'C Cuts at: 0 1571 2427 Size: 1571 856 SapI GCTCTTCn'nnn_ Cuts at: 0 1679 2427 Size: 1679 748 SexAI A'CCwGG_T Cuts at: 0 1734 2427 Size: 1734 693 SfcI C'TryA_G Cuts at: 0 1285 1553 2004 2427 Size: 1285 268 451 423 Fragments arranged by size: 1285 451 423 268 SpeI A'CTAG_T Cuts at: 0 1633 2427 Size: 1633 794 StyI C'CwwG_G Cuts at: 0 114 527 636 786 2091 2273 2427 Size: 114 413 109 150 1305 182 154 Fragments arranged by size: 1305 413 182 154 150 114 109 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 839 866 871 898 1431 1463 2126 2158 Size: 839 27 5 27 533 32 663 32 Cuts at: 2158 2427 Size: 269 Fragments arranged by size: 839 663 533 269 32 32 27 27 5 XbaI T'CTAG_A Cuts at: 0 2395 2427 Size: 2395 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 598 2427 Size: 598 1829 XmnI GAAnn'nnTTC Cuts at: 0 166 2427 Size: 166 2261 Enzymes that do cut: Acc65I AcuI AfeI AleI AlwNI ApoI AseI BamHI BanI BanII BbsI BbvCI BclI BfrBI BglII Bme1580I BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BssSI BstEII BstXI BstYI BtgI DraI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HpaI KpnI MfeI MmeI MslI MspA1I NcoI NsiI PpuMI PstI PvuII SacI SapI SexAI SfcI SpeI StyI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BglI BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DrdI EaeI EagI EciI EcoNI FseI FspI FspAI HindIII KasI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XhoI XmaI ZraI