(Circular) (Six-base) MAPSORT of: pFN21AE2778.seq Check: 2504 from: 1 to: 7048 pFN21AE2778 7048 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4858 278 Size: 53 83 186 936 3322 2468 Fragments arranged by size: 3322 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4391 1937 Size: 2454 4594 Acc65I G'GTAC_C Cuts at: 2705 4369 2705 Size: 1664 5384 AclI AA'CG_TT Cuts at: 1563 5175 5548 1563 Size: 3612 373 3063 Fragments arranged by size: 3612 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2158 4107 4271 5111 6495 6763 1632 Size: 526 1949 164 840 1384 268 1917 Fragments arranged by size: 1949 1917 1384 840 526 268 164 AfeI AGC'GCT Cuts at: 2348 3527 2348 Size: 1179 5869 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6851 AflIII A'CryG_T Cuts at: 1203 5947 6924 1203 Size: 4744 977 1327 Fragments arranged by size: 4744 1327 977 AgeI A'CCGG_T Cuts at: 5879 5879 Size: 7048 AhdI GACnn_n'nnGTC Cuts at: 5777 5777 Size: 7048 AleI CACnn'nnGTG Cuts at: 1166 4277 1166 Size: 3111 3937 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7048 AlwNI CAG_nnn'CTG Cuts at: 4196 4791 6363 4196 Size: 595 1572 4881 Fragments arranged by size: 4881 1572 595 ApaLI G'TGCA_C Cuts at: 5105 6261 5105 Size: 1156 5892 ApoI r'AATT_y Cuts at: 1493 3412 3654 4052 4357 4583 4735 6844 1493 Size: 1919 242 398 305 226 152 2109 1697 Fragments arranged by size: 2109 1919 1697 398 305 242 226 152 AseI AT'TA_AT Cuts at: 160 1032 2514 3284 3944 5602 160 Size: 872 1482 770 660 1658 1606 Fragments arranged by size: 1658 1606 1482 872 770 660 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 AvaI C'yCGr_G Cuts at: 1870 1943 4373 1870 Size: 73 2430 4545 Fragments arranged by size: 4545 2430 73 BamHI G'GATC_C Cuts at: 2393 4378 4745 2393 Size: 1985 367 4696 Fragments arranged by size: 4696 1985 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 2705 3223 4369 5824 Size: 325 217 627 369 549 518 1146 1455 Cuts at: 5824 618 Size: 1842 Fragments arranged by size: 1842 1455 1146 627 549 518 369 325 217 BanII G_rGCy'C Cuts at: 1391 3576 4367 1391 Size: 2185 791 4072 Fragments arranged by size: 4072 2185 791 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3373 4275 4327 928 Size: 971 1474 902 52 3649 Fragments arranged by size: 3649 1474 971 902 52 BbvCI CC'TCA_GC Cuts at: 3542 3542 Size: 7048 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5239 5273 1903 Size: 34 3302 34 3678 Fragments arranged by size: 3678 3302 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4940 6150 4940 Size: 1210 5838 BclI T'GATC_A Cuts at: 1548 3088 3916 1548 Size: 1540 828 4680 Fragments arranged by size: 4680 1540 828 BfrBI ATG'CAT Cuts at: 3239 3420 3239 Size: 181 6867 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5659 136 Size: 107 122 71 631 605 3987 1525 Fragments arranged by size: 3987 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2085 7043 2085 Size: 4958 2090 BlpI GC'TnA_GC Cuts at: 4465 4465 Size: 7048 Bme1580I G_kGCm'C Cuts at: 1790 2140 2159 5109 6265 1790 Size: 350 19 2950 1156 2573 Fragments arranged by size: 2950 2573 1156 350 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7048 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4779 5737 442 Size: 416 53 83 399 24 3362 958 1753 Fragments arranged by size: 3362 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7048 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2915 3609 4245 5708 1239 Size: 1676 694 636 1463 2579 Fragments arranged by size: 2579 1676 1463 694 636 Bpu10I CC'TnA_GC Cuts at: 3542 3542 Size: 7048 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5173 6038 6336 5173 Size: 865 298 5885 Fragments arranged by size: 5885 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5711 882 Size: 617 4212 2219 Fragments arranged by size: 4212 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4839 493 Size: 711 3635 2702 Fragments arranged by size: 3635 2702 711 BsaBI GATnn'nnATC Cuts at: 7042 7042 Size: 7048 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2358 4855 5237 5872 Size: 53 83 186 936 825 2497 382 635 Cuts at: 5872 275 Size: 1451 Fragments arranged by size: 2497 1451 936 825 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5481 5879 6153 6300 6711 5481 Size: 398 274 147 411 5818 Fragments arranged by size: 5818 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2871 2901 2946 2976 3436 Size: 30 165 30 1468 30 45 30 460 Cuts at: 3436 3466 4131 4161 1178 Size: 30 665 30 4065 Fragments arranged by size: 4065 1468 665 460 165 45 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2390 2970 3875 1186 Size: 1204 580 905 4359 Fragments arranged by size: 4359 1204 905 580 BseYI C'CCAG_C Cuts at: 2281 6251 6747 2281 Size: 3970 496 2582 Fragments arranged by size: 3970 2582 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6475 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4498 5259 6287 1540 Size: 275 186 2497 761 1028 2301 Fragments arranged by size: 2497 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2632 3576 5109 5194 6265 2632 Size: 944 1533 85 1071 3415 Fragments arranged by size: 3415 1533 1071 944 85 BsmI GAATG_Cn' Cuts at: 2497 4559 4652 2497 Size: 2062 93 4893 Fragments arranged by size: 4893 2062 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2793 2963 2793 Size: 170 6878 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 2159 2632 3576 4367 5109 5194 Size: 399 350 19 473 944 791 742 85 Cuts at: 5194 6265 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 944 791 742 473 399 350 85 19 BspEI T'CCGG_A Cuts at: 6711 6711 Size: 7048 BspHI T'CATG_A Cuts at: 3846 4937 6667 3846 Size: 1091 1730 4227 Fragments arranged by size: 4227 1730 1091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4403 844 Size: 3559 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4935 1394 Size: 429 3112 3507 Fragments arranged by size: 3507 3112 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5543 5717 65 Size: 1183 4295 174 1396 Fragments arranged by size: 4295 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5692 5879 1700 Size: 110 3882 187 2869 Fragments arranged by size: 3882 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7048 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7048 BssSI C'ACGA_G Cuts at: 2853 4170 5108 6120 2853 Size: 1317 938 1012 3781 Fragments arranged by size: 3781 1317 1012 938 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7048 BstBI TT'CG_AA Cuts at: 5852 5852 Size: 7048 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7048 BstXI CCAn_nnnn'nTGG Cuts at: 2309 2309 Size: 7048 BstYI r'GATC_y Cuts at: 1972 2085 2393 3160 4313 4378 4745 5130 5147 Size: 113 308 767 1153 65 367 385 17 Cuts at: 5147 6599 6685 6697 7043 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1153 767 385 367 346 308 113 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 2641 4096 513 Size: 552 1576 1455 3465 Fragments arranged by size: 3465 1576 1455 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6309 Fragments arranged by size: 6309 683 56 BtsI GCAGTG_nn' Cuts at: 4564 5358 5378 4564 Size: 794 20 6234 Fragments arranged by size: 6234 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7048 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7013 DraI TTT'AAA Cuts at: 3759 4352 3759 Size: 593 6455 DraIII CAC_nnn'GTG Cuts at: 2858 2858 Size: 7048 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6055 809 Size: 507 4739 1802 Fragments arranged by size: 4739 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4495 5384 7013 Size: 54 1425 50 275 18 2665 889 1629 Cuts at: 7013 8 Size: 43 Fragments arranged by size: 2665 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4495 1537 Size: 275 2683 4090 Fragments arranged by size: 4090 2683 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2791 3684 4978 1353 Size: 1438 893 1294 3423 Fragments arranged by size: 3423 1438 1294 893 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5635 6009 6155 387 Size: 1064 138 4046 374 146 1280 Fragments arranged by size: 4046 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3574 3574 Size: 7048 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2158 2915 3609 4107 4245 4271 5111 Size: 393 526 757 694 498 138 26 840 Cuts at: 5111 5708 6495 6763 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 840 787 757 694 597 526 498 393 268 138 26 EcoO109I rG'GnC_Cy Cuts at: 2788 3094 4193 2788 Size: 306 1099 5643 Fragments arranged by size: 5643 1099 306 EcoRI G'AATT_C Cuts at: 4052 4357 4735 4052 Size: 305 378 6365 Fragments arranged by size: 6365 378 305 EcoRV GAT'ATC Cuts at: 2449 2449 Size: 7048 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2188 2413 2759 3338 2188 Size: 225 346 579 5898 Fragments arranged by size: 5898 579 346 225 FspI TGC'GCA Cuts at: 5554 5554 Size: 7048 HaeII r_GCGC'y Cuts at: 1678 2350 3274 3529 6195 1678 Size: 672 924 255 2666 2531 Fragments arranged by size: 2666 2531 924 672 255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2077 2109 2732 2764 2946 Size: 32 135 32 505 32 623 32 182 Cuts at: 2946 2978 5689 5721 5763 5795 1373 Size: 32 2711 32 42 32 2626 Fragments arranged by size: 2711 2626 623 505 182 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2053 2529 4392 4638 669 Size: 988 281 115 476 1863 246 3079 Fragments arranged by size: 3079 1863 988 476 281 246 115 HindIII A'AGCT_T Cuts at: 748 748 Size: 7048 HpaI GTT'AAC Cuts at: 2053 4638 2053 Size: 2585 4463 KpnI G_GTAC'C Cuts at: 2709 4373 2709 Size: 1664 5384 MfeI C'AATT_G Cuts at: 2988 4647 2988 Size: 1659 5389 MluI A'CGCG_T Cuts at: 6924 6924 Size: 7048 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3889 6162 6346 1305 Size: 2584 2273 184 2007 Fragments arranged by size: 2584 2273 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5226 Fragments arranged by size: 5226 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2307 4277 5006 5365 5524 518 Size: 648 1141 1970 729 359 159 2042 Fragments arranged by size: 2042 1970 1141 729 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2762 3398 4464 5141 6289 6534 1606 Size: 1156 636 1066 677 1148 245 2120 Fragments arranged by size: 2120 1156 1148 1066 677 636 245 NcoI C'CATG_G Cuts at: 513 1065 2641 4096 513 Size: 552 1576 1455 3465 Fragments arranged by size: 3465 1576 1455 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7048 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7048 NotI GC'GGCC_GC Cuts at: 4495 4495 Size: 7048 NsiI A_TGCA'T Cuts at: 3241 3422 3241 Size: 181 6867 NspI r_CATG'y Cuts at: 1132 1229 4406 5951 1132 Size: 97 3177 1545 2229 Fragments arranged by size: 3177 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7048 PciI A'CATG_T Cuts at: 5947 5947 Size: 7048 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7048 PmeI GTTT'AAAC Cuts at: 4352 4352 Size: 7048 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5117 5149 6630 6662 5117 Size: 32 1481 32 5503 Fragments arranged by size: 5503 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3094 4193 3094 Size: 1099 5949 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7048 PsiI TTA'TAA Cuts at: 4618 4618 Size: 7048 PstI C_TGCA'G Cuts at: 830 3294 3562 4400 830 Size: 2464 268 838 3478 Fragments arranged by size: 3478 2464 838 268 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7048 PvuII CAG'CTG Cuts at: 2762 3398 2762 Size: 636 6412 SacI G_AGCT'C Cuts at: 3576 3576 Size: 7048 SalI G'TCGA_C Cuts at: 1936 4390 1936 Size: 2454 4594 SapI GCTCTTCn'nnn_ Cuts at: 3684 3684 Size: 7048 SbfI CC_TGCA'GG Cuts at: 4400 4400 Size: 7048 ScaI AGT'ACT Cuts at: 5296 7008 5296 Size: 1712 5336 SexAI A'CCwGG_T Cuts at: 3739 3739 Size: 7048 SfcI C'TryA_G Cuts at: 826 1045 3290 3558 4009 4396 5531 6212 6403 Size: 219 2245 268 451 387 1135 681 191 Cuts at: 6403 826 Size: 1471 Fragments arranged by size: 2245 1471 1135 681 451 387 268 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7048 SmaI CCC'GGG Cuts at: 1872 4375 1872 Size: 2503 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5152 6053 6315 820 Size: 197 926 3209 901 262 1553 Fragments arranged by size: 3209 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4839 493 Size: 4346 2702 SpeI A'CTAG_T Cuts at: 152 3638 152 Size: 3486 3562 SphI G_CATG'C Cuts at: 1132 4406 1132 Size: 3274 3774 SspI AAT'ATT Cuts at: 5 52 4972 5 Size: 47 4920 2081 Fragments arranged by size: 4920 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7048 StyI C'CwwG_G Cuts at: 513 1065 2119 2532 2641 2791 4096 4487 513 Size: 552 1054 413 109 150 1305 391 3074 Fragments arranged by size: 3074 1305 1054 552 413 391 150 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5075 5092 5245 5430 5875 325 Size: 1255 3495 17 153 185 445 1498 Fragments arranged by size: 3495 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5294 7006 96 Size: 274 80 33 51 1443 3317 1712 138 Fragments arranged by size: 3317 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2844 2871 2876 2903 3436 3468 4131 4163 7008 Size: 27 5 27 533 32 663 32 2845 Cuts at: 7008 7040 2844 Size: 32 2852 Fragments arranged by size: 2852 2845 663 533 32 32 32 27 27 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7048 XbaI T'CTAG_A Cuts at: 4316 4384 4316 Size: 68 6980 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2603 2603 Size: 7048 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7048 XmaI C'CCGG_G Cuts at: 1870 4373 1870 Size: 2503 4545 XmnI GAAnn'nnTTC Cuts at: 2171 5177 2171 Size: 3006 4042 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4856 276 Size: 53 83 186 936 3322 2468 Fragments arranged by size: 3322 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI