(Linear) (Six-base) MAPSORT of: KIEE2778.seq Check: 1150 from: 1 to: 2343 KIEE2778.orf With 182 enzymes: SgfI * July 3, 2020 20:21 .. Acc65I G'GTAC_C Cuts at: 0 700 2343 Size: 700 1643 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 2102 2266 2343 Size: 153 1949 164 77 Fragments arranged by size: 1949 164 153 77 AfeI AGC'GCT Cuts at: 0 343 1522 2343 Size: 343 1179 821 Fragments arranged by size: 1179 821 343 AleI CACnn'nnGTG Cuts at: 0 2272 2343 Size: 2272 71 AlwNI CAG_nnn'CTG Cuts at: 0 2191 2343 Size: 2191 152 ApoI r'AATT_y Cuts at: 0 1407 1649 2047 2343 Size: 1407 242 398 296 Fragments arranged by size: 1407 398 296 242 AseI AT'TA_AT Cuts at: 0 509 1279 1939 2343 Size: 509 770 660 404 Fragments arranged by size: 770 660 509 404 BamHI G'GATC_C Cuts at: 0 388 2343 Size: 388 1955 BanI G'GyrC_C Cuts at: 0 151 700 1218 2343 Size: 151 549 518 1125 Fragments arranged by size: 1125 549 518 151 BanII G_rGCy'C Cuts at: 0 1571 2343 Size: 1571 772 BbsI GAAGACnn'nnnn_ Cuts at: 0 1368 2270 2322 2343 Size: 1368 902 52 21 Fragments arranged by size: 1368 902 52 21 BbvCI CC'TCA_GC Cuts at: 0 1537 2343 Size: 1537 806 BclI T'GATC_A Cuts at: 0 1083 1911 2343 Size: 1083 828 432 Fragments arranged by size: 1083 828 432 BfrBI ATG'CAT Cuts at: 0 1234 1415 2343 Size: 1234 181 928 Fragments arranged by size: 1234 928 181 BglII A'GATC_T Cuts at: 0 80 2343 Size: 80 2263 Bme1580I G_kGCm'C Cuts at: 0 135 154 2343 Size: 135 19 2189 Fragments arranged by size: 2189 135 19 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 910 1604 2240 2343 Size: 910 694 636 103 Fragments arranged by size: 910 694 636 103 Bpu10I CC'TnA_GC Cuts at: 0 1537 2343 Size: 1537 806 BsaHI Gr'CG_yC Cuts at: 0 353 2343 Size: 353 1990 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 866 896 941 971 1431 1461 2126 2156 Size: 866 30 45 30 460 30 665 30 Cuts at: 2156 2343 Size: 187 Fragments arranged by size: 866 665 460 187 45 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 385 965 1870 2343 Size: 385 580 905 473 Fragments arranged by size: 905 580 473 385 BseYI C'CCAG_C Cuts at: 0 276 2343 Size: 276 2067 BsiHKAI G_wGCw'C Cuts at: 0 627 1571 2343 Size: 627 944 772 Fragments arranged by size: 944 772 627 BsmI GAATG_Cn' Cuts at: 0 492 2343 Size: 492 1851 BsmBI CGTCTCn'nnnn_ Cuts at: 0 788 958 2343 Size: 788 170 1385 Fragments arranged by size: 1385 788 170 Bsp1286I G_dGCh'C Cuts at: 0 135 154 627 1571 2343 Size: 135 19 473 944 772 Fragments arranged by size: 944 772 473 135 19 BspHI T'CATG_A Cuts at: 0 1841 2343 Size: 1841 502 BssSI C'ACGA_G Cuts at: 0 848 2165 2343 Size: 848 1317 178 Fragments arranged by size: 1317 848 178 BstXI CCAn_nnnn'nTGG Cuts at: 0 304 2343 Size: 304 2039 BstYI r'GATC_y Cuts at: 0 80 388 1155 2308 2343 Size: 80 308 767 1153 35 Fragments arranged by size: 1153 767 308 80 35 BtgI C'CryG_G Cuts at: 0 636 2091 2343 Size: 636 1455 252 Fragments arranged by size: 1455 636 252 DraI TTT'AAA Cuts at: 0 1754 2343 Size: 1754 589 DraIII CAC_nnn'GTG Cuts at: 0 853 2343 Size: 853 1490 EarI CTCTTCn'nnn_ Cuts at: 0 786 1679 2343 Size: 786 893 664 Fragments arranged by size: 893 786 664 EcoICRI GAG'CTC Cuts at: 0 1569 2343 Size: 1569 774 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 910 1604 2102 2240 2266 2343 Size: 153 757 694 498 138 26 77 Fragments arranged by size: 757 694 498 153 138 77 26 EcoO109I rG'GnC_Cy Cuts at: 0 783 1089 2188 2343 Size: 783 306 1099 155 Fragments arranged by size: 1099 783 306 155 EcoRI G'AATT_C Cuts at: 0 2047 2343 Size: 2047 296 EcoRV GAT'ATC Cuts at: 0 444 2343 Size: 444 1899 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 183 408 754 1333 2343 Size: 183 225 346 579 1010 Fragments arranged by size: 1010 579 346 225 183 HaeII r_GCGC'y Cuts at: 0 345 1269 1524 2343 Size: 345 924 255 819 Fragments arranged by size: 924 819 345 255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 72 104 727 759 941 973 2343 Size: 72 32 623 32 182 32 1370 Fragments arranged by size: 1370 623 182 72 32 32 32 HincII GTy'rAC Cuts at: 0 48 524 2343 Size: 48 476 1819 Fragments arranged by size: 1819 476 48 HpaI GTT'AAC Cuts at: 0 48 2343 Size: 48 2295 KpnI G_GTAC'C Cuts at: 0 704 2343 Size: 704 1639 MfeI C'AATT_G Cuts at: 0 983 2343 Size: 983 1360 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1884 2343 Size: 1884 459 MslI CAynn'nnrTG Cuts at: 0 302 2272 2343 Size: 302 1970 71 Fragments arranged by size: 1970 302 71 MspA1I CmG'CkG Cuts at: 0 757 1393 2343 Size: 757 636 950 Fragments arranged by size: 950 757 636 NcoI C'CATG_G Cuts at: 0 636 2091 2343 Size: 636 1455 252 Fragments arranged by size: 1455 636 252 NsiI A_TGCA'T Cuts at: 0 1236 1417 2343 Size: 1236 181 926 Fragments arranged by size: 1236 926 181 PpuMI rG'GwC_Cy Cuts at: 0 1089 2188 2343 Size: 1089 1099 155 Fragments arranged by size: 1099 1089 155 PstI C_TGCA'G Cuts at: 0 1289 1557 2343 Size: 1289 268 786 Fragments arranged by size: 1289 786 268 PvuII CAG'CTG Cuts at: 0 757 1393 2343 Size: 757 636 950 Fragments arranged by size: 950 757 636 SacI G_AGCT'C Cuts at: 0 1571 2343 Size: 1571 772 SapI GCTCTTCn'nnn_ Cuts at: 0 1679 2343 Size: 1679 664 SexAI A'CCwGG_T Cuts at: 0 1734 2343 Size: 1734 609 SfcI C'TryA_G Cuts at: 0 1285 1553 2004 2343 Size: 1285 268 451 339 Fragments arranged by size: 1285 451 339 268 SpeI A'CTAG_T Cuts at: 0 1633 2343 Size: 1633 710 StyI C'CwwG_G Cuts at: 0 114 527 636 786 2091 2343 Size: 114 413 109 150 1305 252 Fragments arranged by size: 1305 413 252 150 114 109 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 839 866 871 898 1431 1463 2126 2158 Size: 839 27 5 27 533 32 663 32 Cuts at: 2158 2343 Size: 185 Fragments arranged by size: 839 663 533 185 32 32 27 27 5 XbaI T'CTAG_A Cuts at: 0 2311 2343 Size: 2311 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 598 2343 Size: 598 1745 XmnI GAAnn'nnTTC Cuts at: 0 166 2343 Size: 166 2177 Enzymes that do cut: Acc65I AcuI AfeI AleI AlwNI ApoI AseI BamHI BanI BanII BbsI BbvCI BclI BfrBI BglII Bme1580I BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BssSI BstXI BstYI BtgI DraI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HpaI KpnI MfeI MmeI MslI MspA1I NcoI NsiI PpuMI PstI PvuII SacI SapI SexAI SfcI SpeI StyI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BglI BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DrdI EaeI EagI EciI EcoNI FseI FspI FspAI HindIII KasI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XhoI XmaI ZraI