(Circular) (Six-base) MAPSORT of: pFN21AE2782.seq Check: 819 from: 1 to: 6055 pFN21AE2782 6055 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3865 278 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2349 3398 1937 Size: 412 1049 4594 Fragments arranged by size: 4594 1049 412 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2173 2177 2713 2983 4118 5502 5770 1632 Size: 541 4 536 270 1135 1384 268 1917 Fragments arranged by size: 1917 1384 1135 541 536 270 268 4 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5858 AflIII A'CryG_T Cuts at: 1203 4954 5931 1203 Size: 3751 977 1327 Fragments arranged by size: 3751 1327 977 AgeI A'CCGG_T Cuts at: 4886 4886 Size: 6055 AhdI GACnn_n'nnGTC Cuts at: 4784 4784 Size: 6055 AleI CACnn'nnGTG Cuts at: 1166 2630 3047 1166 Size: 1464 417 4174 Fragments arranged by size: 4174 1464 417 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2711 1184 Size: 1527 4528 AlwNI CAG_nnn'CTG Cuts at: 2044 3093 3312 3798 5370 2044 Size: 1049 219 486 1572 2729 Fragments arranged by size: 2729 1572 1049 486 219 ApaI G_GGCC'C Cuts at: 2511 2639 2511 Size: 128 5927 ApaLI G'TGCA_C Cuts at: 4112 5268 4112 Size: 1156 4899 ApoI r'AATT_y Cuts at: 1493 2027 3364 3590 3742 5851 1493 Size: 534 1337 226 152 2109 1697 Fragments arranged by size: 2109 1697 1337 534 226 152 AseI AT'TA_AT Cuts at: 160 1032 4609 160 Size: 872 3577 1606 Fragments arranged by size: 3577 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 2138 3380 1870 Size: 73 195 1242 4545 Fragments arranged by size: 4545 1242 195 73 BamHI G'GATC_C Cuts at: 3081 3385 3752 3081 Size: 304 367 5384 Fragments arranged by size: 5384 367 304 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2449 2492 3111 3376 4831 Size: 325 217 627 662 43 619 265 1455 Cuts at: 4831 618 Size: 1842 Fragments arranged by size: 1842 1455 662 627 619 325 265 217 43 BanII G_rGCy'C Cuts at: 1391 2511 2639 3184 3215 3297 3374 1391 Size: 1120 128 545 31 82 77 4072 Fragments arranged by size: 4072 1120 545 128 82 77 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2287 2494 928 Size: 971 388 207 4489 Fragments arranged by size: 4489 971 388 207 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3119 3153 4246 4280 1903 Size: 34 1182 34 1093 34 3678 Fragments arranged by size: 3678 1182 1093 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3947 5157 3947 Size: 1210 4845 BclI T'GATC_A Cuts at: 1548 1548 Size: 6055 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2402 2774 4666 Size: 107 122 71 631 605 730 372 1892 Cuts at: 4666 136 Size: 1525 Fragments arranged by size: 1892 1525 730 631 605 372 122 107 71 BglII A'GATC_T Cuts at: 2088 6050 2088 Size: 3962 2093 BlpI GC'TnA_GC Cuts at: 2237 3296 3472 2237 Size: 1059 176 4820 Fragments arranged by size: 4820 1059 176 Bme1580I G_kGCm'C Cuts at: 1790 2383 2454 2495 2511 2639 3116 4116 5272 Size: 593 71 41 16 128 477 1000 1156 Cuts at: 5272 1790 Size: 2573 Fragments arranged by size: 2573 1156 1000 593 477 128 71 41 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6055 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3322 3786 4744 Size: 416 53 83 399 24 1905 464 958 Cuts at: 4744 442 Size: 1753 Fragments arranged by size: 1905 1753 958 464 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2152 4715 1239 Size: 913 2563 2579 Fragments arranged by size: 2579 2563 913 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4180 5045 5343 4180 Size: 865 298 4892 Fragments arranged by size: 4892 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4718 882 Size: 617 3219 2219 Fragments arranged by size: 3219 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3049 3846 493 Size: 711 1845 797 2702 Fragments arranged by size: 2702 1845 797 711 BsaBI GATnn'nnATC Cuts at: 6049 6049 Size: 6055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2292 3862 4244 4879 Size: 53 83 186 936 759 1570 382 635 Cuts at: 4879 275 Size: 1451 Fragments arranged by size: 1570 1451 936 759 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3063 4488 4886 5160 5307 5718 3063 Size: 1425 398 274 147 411 3400 Fragments arranged by size: 3400 1425 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3197 3227 1178 Size: 30 165 30 1794 30 4006 Fragments arranged by size: 4006 1794 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2810 3263 1186 Size: 931 693 453 3978 Fragments arranged by size: 3978 931 693 453 BseYI C'CCAG_C Cuts at: 2070 3300 5258 5754 2070 Size: 1230 1958 496 2371 Fragments arranged by size: 2371 1958 1230 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 1163 Size: 573 462 5020 Fragments arranged by size: 5020 573 462 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3505 4266 5294 1540 Size: 275 186 1504 761 1028 2301 Fragments arranged by size: 2301 1504 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3184 3297 4116 4201 5272 3184 Size: 113 819 85 1071 3967 Fragments arranged by size: 3967 1071 819 113 85 BsmI GAATG_Cn' Cuts at: 2935 3566 3659 2935 Size: 631 93 5331 Fragments arranged by size: 5331 631 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3350 3350 Size: 6055 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2383 2454 2495 2511 2639 3116 3184 Size: 399 593 71 41 16 128 477 68 Cuts at: 3184 3215 3297 3374 4116 4201 5272 1391 Size: 31 82 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 593 477 399 128 85 82 77 71 68 41 31 16 BspEI T'CCGG_A Cuts at: 3063 5718 3063 Size: 2655 3400 BspHI T'CATG_A Cuts at: 3944 5674 3944 Size: 1730 4325 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2898 3410 844 Size: 2054 512 3489 Fragments arranged by size: 3489 2054 512 BsrBI CCG'CTC Cuts at: 1394 1823 3942 1394 Size: 429 2119 3507 Fragments arranged by size: 3507 2119 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4550 4724 65 Size: 1183 3302 174 1396 Fragments arranged by size: 3302 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2565 4699 4886 1700 Size: 110 755 2134 187 2869 Fragments arranged by size: 2869 2134 755 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 2255 4115 5127 2255 Size: 1860 1012 3183 Fragments arranged by size: 3183 1860 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2044 2371 794 Size: 1250 327 4478 Fragments arranged by size: 4478 1250 327 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 2672 2965 1184 Size: 1488 293 4274 Fragments arranged by size: 4274 1488 293 BstXI CCAn_nnnn'nTGG Cuts at: 2632 2632 Size: 6055 BstYI r'GATC_y Cuts at: 1972 2088 3081 3333 3385 3752 4137 4154 5606 Size: 116 993 252 52 367 385 17 1452 Cuts at: 5606 5692 5704 6050 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 993 385 367 346 252 116 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2172 2763 513 Size: 552 939 168 591 3805 Fragments arranged by size: 3805 939 591 552 168 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3122 487 Size: 683 56 1896 3420 Fragments arranged by size: 3420 1896 683 56 BtsI GCAGTG_nn' Cuts at: 2412 3571 4365 4385 2412 Size: 1159 794 20 4082 Fragments arranged by size: 4082 1159 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2154 2189 529 Size: 35 1590 35 4395 Fragments arranged by size: 4395 1590 35 35 DraI TTT'AAA Cuts at: 3359 3359 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5062 809 Size: 507 3746 1802 Fragments arranged by size: 3746 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2394 2766 Size: 54 1425 50 275 18 177 387 372 Cuts at: 2766 3186 3502 4391 6020 8 Size: 420 316 889 1629 43 Fragments arranged by size: 1629 1425 889 420 387 372 316 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3502 1537 Size: 275 1690 4090 Fragments arranged by size: 4090 1690 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2651 3985 1353 Size: 1298 1334 3423 Fragments arranged by size: 3423 1334 1298 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2927 4642 5016 5162 387 Size: 1064 138 1338 1715 374 146 1280 Fragments arranged by size: 1715 1338 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3182 3295 3182 Size: 113 5942 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2152 2173 2177 2713 2983 4118 4715 Size: 393 520 21 4 536 270 1135 597 Cuts at: 4715 5502 5770 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1135 787 597 536 520 393 270 268 21 4 EcoO109I rG'GnC_Cy Cuts at: 2077 2508 2636 2818 2946 2077 Size: 431 128 182 128 5186 Fragments arranged by size: 5186 431 182 128 128 EcoRI G'AATT_C Cuts at: 3364 3742 3364 Size: 378 5677 EcoRV GAT'ATC Cuts at: 2517 2517 Size: 6055 FspI TGC'GCA Cuts at: 4561 4561 Size: 6055 HaeII r_GCGC'y Cuts at: 1678 2985 3073 5202 1678 Size: 1307 88 2129 2531 Fragments arranged by size: 2531 2129 1307 88 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3318 3350 4696 4728 4770 Size: 32 135 32 1746 32 1346 32 42 Cuts at: 4770 4802 1373 Size: 32 2626 Fragments arranged by size: 2626 1746 1346 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3399 3645 669 Size: 988 281 1461 246 3079 Fragments arranged by size: 3079 1461 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6055 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5169 5353 1305 Size: 3864 184 2007 Fragments arranged by size: 3864 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2396 3188 10 Size: 54 1425 343 177 387 792 2877 Fragments arranged by size: 2877 1425 792 387 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2630 3047 4013 4372 4531 518 Size: 648 1464 417 966 359 159 2042 Fragments arranged by size: 2042 1464 966 648 417 359 159 MspA1I CmG'CkG Cuts at: 1606 2212 3300 3471 4148 5296 5541 1606 Size: 606 1088 171 677 1148 245 2120 Fragments arranged by size: 2120 1148 1088 677 606 245 171 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4564 Fragments arranged by size: 4564 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 2165 3059 3413 4958 1132 Size: 97 936 894 354 1545 2229 Fragments arranged by size: 2229 1545 936 894 354 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6055 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 3312 1108 Size: 2204 3851 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4124 4156 5637 5669 4124 Size: 32 1481 32 4510 Fragments arranged by size: 4510 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2818 2946 2818 Size: 128 5927 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6055 PsiI TTA'TAA Cuts at: 3625 3625 Size: 6055 PspOMI G'GGCC_C Cuts at: 2507 2635 2507 Size: 128 5927 PstI C_TGCA'G Cuts at: 830 2577 3407 830 Size: 1747 830 3478 Fragments arranged by size: 3478 1747 830 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 PvuII CAG'CTG Cuts at: 2212 3300 2212 Size: 1088 4967 SacI G_AGCT'C Cuts at: 3184 3297 3184 Size: 113 5942 SalI G'TCGA_C Cuts at: 1936 3397 1936 Size: 1461 4594 SbfI CC_TGCA'GG Cuts at: 3407 3407 Size: 6055 ScaI AGT'ACT Cuts at: 4303 6015 4303 Size: 1712 4343 SfcI C'TryA_G Cuts at: 826 1045 2573 3403 4538 5219 5410 826 Size: 219 1528 830 1135 681 191 1471 Fragments arranged by size: 1528 1471 1135 830 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6055 SmaI CCC'GGG Cuts at: 1872 3382 1872 Size: 1510 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4159 5060 5322 820 Size: 197 926 2216 901 262 1553 Fragments arranged by size: 2216 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 2165 3413 1132 Size: 1033 1248 3774 Fragments arranged by size: 3774 1248 1033 SspI AAT'ATT Cuts at: 5 52 3979 5 Size: 47 3927 2081 Fragments arranged by size: 3927 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6055 StyI C'CwwG_G Cuts at: 513 1065 2004 2545 3494 513 Size: 552 939 541 949 3074 Fragments arranged by size: 3074 949 939 552 541 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2321 2847 4082 4099 4252 4437 4882 Size: 1255 741 526 1235 17 153 185 445 Cuts at: 4882 325 Size: 1498 Fragments arranged by size: 1498 1255 1235 741 526 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4301 6013 96 Size: 274 80 33 51 1443 2324 1712 138 Fragments arranged by size: 2324 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3073 3105 6015 6047 3073 Size: 32 2910 32 3081 Fragments arranged by size: 3081 2910 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2827 1316 Size: 1511 4544 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2770 3196 2770 Size: 426 5629 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6055 XmaI C'CCGG_G Cuts at: 1870 3380 1870 Size: 1510 4545 XmnI GAAnn'nnTTC Cuts at: 4184 4184 Size: 6055 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3863 276 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI