(Linear) (Six-base) MAPSORT of: KIEE2782.seq Check: 1796 from: 1 to: 1350 KIEE2782.orf With 182 enzymes: SgfI * February 19, 2021 20:23 .. AccI GT'mk_AC Cuts at: 0 344 1350 Size: 344 1006 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 168 172 708 978 1350 Size: 168 4 536 270 372 Fragments arranged by size: 536 372 270 168 4 AleI CACnn'nnGTG Cuts at: 0 625 1042 1350 Size: 625 417 308 Fragments arranged by size: 625 417 308 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 706 1350 Size: 706 644 AlwNI CAG_nnn'CTG Cuts at: 0 39 1088 1307 1350 Size: 39 1049 219 43 Fragments arranged by size: 1049 219 43 39 ApaI G_GGCC'C Cuts at: 0 506 634 1350 Size: 506 128 716 Fragments arranged by size: 716 506 128 ApoI r'AATT_y Cuts at: 0 22 1350 Size: 22 1328 AvaI C'yCGr_G Cuts at: 0 133 1350 Size: 133 1217 BamHI G'GATC_C Cuts at: 0 1076 1350 Size: 1076 274 BanI G'GyrC_C Cuts at: 0 444 487 1106 1350 Size: 444 43 619 244 Fragments arranged by size: 619 444 244 43 BanII G_rGCy'C Cuts at: 0 506 634 1179 1210 1292 1350 Size: 506 128 545 31 82 58 Fragments arranged by size: 545 506 128 82 58 31 BbsI GAAGACnn'nnnn_ Cuts at: 0 282 489 1350 Size: 282 207 861 Fragments arranged by size: 861 282 207 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1114 1148 1350 Size: 1114 34 202 Fragments arranged by size: 1114 202 34 BglI GCCn_nnn'nGGC Cuts at: 0 397 769 1350 Size: 397 372 581 Fragments arranged by size: 581 397 372 BglII A'GATC_T Cuts at: 0 83 1350 Size: 83 1267 BlpI GC'TnA_GC Cuts at: 0 232 1291 1350 Size: 232 1059 59 Fragments arranged by size: 1059 232 59 Bme1580I G_kGCm'C Cuts at: 0 378 449 490 506 634 1111 1350 Size: 378 71 41 16 128 477 239 Fragments arranged by size: 477 378 239 128 71 41 16 BmrI ACTGGGnnnn_n' Cuts at: 0 1317 1350 Size: 1317 33 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 147 1350 Size: 147 1203 BsaAI yAC'GTr Cuts at: 0 1044 1350 Size: 1044 306 BsaHI Gr'CG_yC Cuts at: 0 287 1350 Size: 287 1063 BsaWI w'CCGG_w Cuts at: 0 1058 1350 Size: 1058 292 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1192 1222 1350 Size: 1192 30 128 Fragments arranged by size: 1192 128 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 112 805 1258 1350 Size: 112 693 453 92 Fragments arranged by size: 693 453 112 92 BseYI C'CCAG_C Cuts at: 0 65 1295 1350 Size: 65 1230 55 Fragments arranged by size: 1230 65 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 1350 Size: 193 1157 BsiHKAI G_wGCw'C Cuts at: 0 1179 1292 1350 Size: 1179 113 58 Fragments arranged by size: 1179 113 58 BsmI GAATG_Cn' Cuts at: 0 930 1350 Size: 930 420 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1345 1350 Size: 1345 5 Bsp1286I G_dGCh'C Cuts at: 0 378 449 490 506 634 1111 1179 1210 Size: 378 71 41 16 128 477 68 31 Cuts at: 1210 1292 1350 Size: 82 58 Fragments arranged by size: 477 378 128 82 71 68 58 41 31 16 BspEI T'CCGG_A Cuts at: 0 1058 1350 Size: 1058 292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 893 1350 Size: 893 457 BsrFI r'CCGG_y Cuts at: 0 560 1350 Size: 560 790 BssSI C'ACGA_G Cuts at: 0 250 1350 Size: 250 1100 BstAPI GCAn_nnn'nTGC Cuts at: 0 39 366 1350 Size: 39 327 984 Fragments arranged by size: 984 327 39 BstEII G'GTnAC_C Cuts at: 0 667 960 1350 Size: 667 293 390 Fragments arranged by size: 667 390 293 BstXI CCAn_nnnn'nTGG Cuts at: 0 627 1350 Size: 627 723 BstYI r'GATC_y Cuts at: 0 83 1076 1328 1350 Size: 83 993 252 22 Fragments arranged by size: 993 252 83 22 BtgI C'CryG_G Cuts at: 0 167 758 1350 Size: 167 591 592 Fragments arranged by size: 592 591 167 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1117 1350 Size: 1117 233 BtsI GCAGTG_nn' Cuts at: 0 407 1350 Size: 407 943 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 149 184 1350 Size: 149 35 1166 Fragments arranged by size: 1166 149 35 EaeI y'GGCC_r Cuts at: 0 2 389 761 1181 1350 Size: 2 387 372 420 169 Fragments arranged by size: 420 387 372 169 2 EarI CTCTTCn'nnn_ Cuts at: 0 646 1350 Size: 646 704 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 922 1350 Size: 922 428 EcoICRI GAG'CTC Cuts at: 0 1177 1290 1350 Size: 1177 113 60 Fragments arranged by size: 1177 113 60 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 147 168 172 708 978 1350 Size: 147 21 4 536 270 372 Fragments arranged by size: 536 372 270 147 21 4 EcoO109I rG'GnC_Cy Cuts at: 0 72 503 631 813 941 1350 Size: 72 431 128 182 128 409 Fragments arranged by size: 431 409 182 128 128 72 EcoRV GAT'ATC Cuts at: 0 512 1350 Size: 512 838 HaeII r_GCGC'y Cuts at: 0 980 1068 1350 Size: 980 88 282 Fragments arranged by size: 980 282 88 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1313 1345 1350 Size: 1313 32 5 Fragments arranged by size: 1313 32 5 MscI TGG'CCA Cuts at: 0 4 391 1183 1350 Size: 4 387 792 167 Fragments arranged by size: 792 387 167 4 MslI CAynn'nnrTG Cuts at: 0 625 1042 1350 Size: 625 417 308 Fragments arranged by size: 625 417 308 MspA1I CmG'CkG Cuts at: 0 207 1295 1350 Size: 207 1088 55 Fragments arranged by size: 1088 207 55 NspI r_CATG'y Cuts at: 0 160 1054 1350 Size: 160 894 296 Fragments arranged by size: 894 296 160 PflMI CCAn_nnn'nTGG Cuts at: 0 1307 1350 Size: 1307 43 PpuMI rG'GwC_Cy Cuts at: 0 813 941 1350 Size: 813 128 409 Fragments arranged by size: 813 409 128 PspOMI G'GGCC_C Cuts at: 0 502 630 1350 Size: 502 128 720 Fragments arranged by size: 720 502 128 PstI C_TGCA'G Cuts at: 0 572 1350 Size: 572 778 PvuII CAG'CTG Cuts at: 0 207 1295 1350 Size: 207 1088 55 Fragments arranged by size: 1088 207 55 SacI G_AGCT'C Cuts at: 0 1179 1292 1350 Size: 1179 113 58 Fragments arranged by size: 1179 113 58 SfcI C'TryA_G Cuts at: 0 568 1350 Size: 568 782 SphI G_CATG'C Cuts at: 0 160 1350 Size: 160 1190 StyI C'CwwG_G Cuts at: 0 540 1350 Size: 540 810 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 316 842 1350 Size: 316 526 508 Fragments arranged by size: 526 508 316 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1068 1100 1350 Size: 1068 32 250 Fragments arranged by size: 1068 250 32 Tth111I GACn'n_nGTC Cuts at: 0 822 1350 Size: 822 528 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 765 1191 1350 Size: 765 426 159 Fragments arranged by size: 765 426 159 Enzymes that do cut: AccI AcuI AleI AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BcgI BglI BglII BlpI Bme1580I BmrI BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I MscI MslI MspA1I NspI PflMI PpuMI PspOMI PstI PvuII SacI SfcI SphI StyI TaqII TstI Tth111I XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BclI BfrBI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsiEI BsiWI BspHI BsrBI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI EcoNI EcoRI FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI TatI XbaI XhoI XmaI XmnI ZraI