Restriction map for
pFN21AE2588
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# NO CUT ENZYMES ( 72 enzymes) #
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AclI AflII AscI AviII AvrII
BbeI BciVI BclI BfrI BglII
BlnI BsaBI BsiWI BsmI BspDI
BspHI BspLU11I BssHII BssSI BstAPI
BstBI ClaI CpoI DraI DrdI
EagI EclXI Eco52I EcoRV EcoT22I
FbaI FseI FspI HgaI HpaI
KasI MamI MfeI MluI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PmeI Psp1406I PvuI
RcaI RsrII SalI SbfI SexAI
SfiI SfoI SfuI SmaI SnaBI
SpeI SphI Sse8387I SwaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 1228 GACGTC GACGT^C
Acc65I 916 GGTACC G^GTACC
AccI 2720 GTATAC GT^[AC][GT]AC
AccIII 1361 TCCGGA T^CCGGA
AcsI 2208 GAATTC [GA]^AATT[CT]
AcyI 1228 GACGTC G[GA]^CG[CT]C
AgeI 976 ACCGGT A^CCGGT
AhdI 2257 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1495 GTGCAC G^TGCAC
Aor51HI 186 AGCGCT AGC^GCT
ApaLI 1495 GTGCAC G^TGCAC
ApoI 2208 GAATTC [GA]^AATT[CT]
AseI 1315 ATTAAT AT^TAAT
AsnI 1315 ATTAAT AT^TAAT
Asp700I 2041 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 916 GGTACC G^GTACC
AspEI 2257 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1468 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1349 CCCGAG C^[CT]CG[GA]G
BbrPI 2571 CACGTG CAC^GTG
BbsI 196 GAAGAC GAAGAC(2/6)
BcgI 1900 GCAGGCCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 196 GAAGAC GAAGAC(2/6)
BsaHI 1228 GACGTC G[GA]^CG[CT]C
BseAI 1361 TCCGGA T^CCGGA
BsmBI 2702 GAGACG CGTCTC(1/5)
BsoBI 1349 CCCGAG C^[CT]CG[GA]G
Bsp1407I 247 TGTACA T^GTACA
BspEI 1361 TCCGGA T^CCGGA
BsrDI 1235 GCAATG GCAATG(2/0)
BsrGI 247 TGTACA T^GTACA
Bst1107I 2720 GTATAC GTA^TAC
BstEII 2610 GGTCACC G^GT[ACGT]ACC
BstPI 2610 GGTCACC G^GT[ACGT]ACC
BstZ17I 2720 GTATAC GTA^TAC
DraIII 1339 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2257 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 186 AGCGCT AGC^GCT
EcoO65I 2610 GGTCACC G^GT[ACGT]ACC
EcoRI 2208 GAATTC G^AATTC
Hin1I 1228 GACGTC G[GA]^CG[CT]C
HincII 2413 GTTGAC GT[CT]^[GA]AC
HindII 2413 GTTGAC GT[CT]^[GA]AC
HindIII 449 AAGCTT A^AGCTT
KpnI 916 GGTACC GGTAC^C
KspI 301 CCGCGG CCGC^GG
MroI 1361 TCCGGA T^CCGGA
NspI 1688 GCATGT [GA]CATG^[CT]
PflFI 1468 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 976 ACCGGT A^CCGGT
PmaCI 2571 CACGTG CAC^GTG
PmlI 2571 CACGTG CAC^GTG
PshAI 717 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1315 ATTAAT AT^TAAT
SacII 301 CCGCGG CCGC^GG
ScaI 2981 AGTACT AGT^ACT
SgrAI 975 CACCGGTG C[GA]^CCGG[CT]G
SspBI 247 TGTACA T^GTACA
SspI 241 AATATT AAT^ATT
Tth111I 1468 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1926 TCTAGA T^CTAGA
XmnI 2041 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AocI 344 CCTCAGG CC^T[ACGT]AGG
2563 CCTGAGG
ApaI 81 GGGCCC GGGCC^C
2754 GGGCCC
BamHI 1305 GGATCC G^GATCC
3126 GGATCC
BglI 2846 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3008 GCCCGCTGGGC
BlpI 103 GCTGAGC GC^T[ACGT]AGC
2875 GCTGAGC
Bpu1102I 103 GCTGAGC GC^T[ACGT]AGC
2875 GCTGAGC
BsaAI 787 TACGTG [CT]AC^GT[GA]
2571 CACGTG
BsaI 2105 GAGACC GGTCTC(1/5)
711 GGTCTC
BsiEI 1956 CGACCG CG[GA][CT]^CG
3282 CGACCG
BsrFI 976 ACCGGT [GA]^CCGG[CT]
2401 ACCGGC
Bsu36I 344 CCTCAGG CC^T[ACGT]AGG
2563 CCTGAGG
CelII 103 GCTGAGC GC^T[ACGT]AGC
2875 GCTGAGC
Cfr10I 976 ACCGGT [GA]^CCGG[CT]
2401 ACCGGC
Eco81I 344 CCTCAGG CC^T[ACGT]AGG
2563 CCTGAGG
EcoNI 543 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1453 CCTGCTCCAGG
MslI 594 CACGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2619 CACGCCCGTG
PflMI 1869 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2915 CCAGACCCTGG
PstI 1969 CTGCAG CTGCA^G
2653 CTGCAG
SapI 902 GAAGAGC GCTCTTC(1/4)
1963 GAAGAGC
StuI 1902 AGGCCT AGG^CCT
3369 AGGCCT
Van91I 1869 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2915 CCAGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII