(Circular) (Six-base) MAPSORT of: pFN21AE2588.seq Check: 8215 from: 1 to: 8134 pFN21AE2588 8134 bp With 182 enzymes: SgfI * September 18, 2015 18:03 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3237 5944 278 Size: 53 83 186 936 1701 2707 2468 Fragments arranged by size: 2707 2468 1701 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4726 5477 1937 Size: 2789 751 4594 Fragments arranged by size: 4594 2789 751 Acc65I G'GTAC_C Cuts at: 2921 5455 2921 Size: 2534 5600 AclI AA'CG_TT Cuts at: 1563 6261 6634 1563 Size: 4698 373 3063 Fragments arranged by size: 4698 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2220 3672 6197 7581 7849 1632 Size: 588 1452 2525 1384 268 1917 Fragments arranged by size: 2525 1917 1452 1384 588 268 AfeI AGC'GCT Cuts at: 2193 2193 Size: 8134 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7937 AflIII A'CryG_T Cuts at: 1203 2793 4540 4575 7033 8010 1203 Size: 1590 1747 35 2458 977 1327 Fragments arranged by size: 2458 1747 1590 1327 977 35 AgeI A'CCGG_T Cuts at: 2981 6965 2981 Size: 3984 4150 AhdI GACnn_n'nnGTC Cuts at: 4267 6863 4267 Size: 2596 5538 AleI CACnn'nnGTG Cuts at: 1166 2603 4628 1166 Size: 1437 2025 4672 Fragments arranged by size: 4672 2025 1437 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2289 3555 1184 Size: 1105 1266 5763 Fragments arranged by size: 5763 1266 1105 AlwNI CAG_nnn'CTG Cuts at: 2175 2488 2531 2645 3517 4926 5877 7449 2175 Size: 313 43 114 872 1409 951 1572 2860 Fragments arranged by size: 2860 1572 1409 951 872 313 114 43 ApaI G_GGCC'C Cuts at: 2090 4763 2090 Size: 2673 5461 ApaLI G'TGCA_C Cuts at: 3500 6191 7347 3500 Size: 2691 1156 4287 Fragments arranged by size: 4287 2691 1156 ApoI r'AATT_y Cuts at: 1493 4213 5443 5669 5821 7930 1493 Size: 2720 1230 226 152 2109 1697 Fragments arranged by size: 2720 2109 1697 1230 226 152 AseI AT'TA_AT Cuts at: 160 1032 3321 6688 160 Size: 872 2289 3367 1606 Fragments arranged by size: 3367 2289 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8134 AvaI C'yCGr_G Cuts at: 1870 1943 3354 5459 1870 Size: 73 1411 2105 4545 Fragments arranged by size: 4545 2105 1411 73 BamHI G'GATC_C Cuts at: 3310 5131 5464 5831 3310 Size: 1821 333 367 5613 Fragments arranged by size: 5613 1821 367 333 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2747 2921 4409 4679 5381 Size: 325 217 627 960 174 1488 270 702 Cuts at: 5381 5455 6910 618 Size: 74 1455 1842 Fragments arranged by size: 1842 1488 1455 960 702 627 325 270 217 174 74 BanII G_rGCy'C Cuts at: 1391 2090 2115 2216 2466 2706 3132 3578 4027 Size: 699 25 101 250 240 426 446 449 Cuts at: 4027 4334 4755 4763 4887 4941 5015 5313 5453 Size: 307 421 8 124 54 74 298 140 Cuts at: 5453 1391 Size: 4072 Fragments arranged by size: 4072 699 449 446 426 421 307 298 250 240 140 124 101 74 54 25 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2208 928 Size: 971 309 6854 Fragments arranged by size: 6854 971 309 BbvCI CC'TCA_GC Cuts at: 2131 2549 3297 3453 3669 4141 2131 Size: 418 748 156 216 472 6124 Fragments arranged by size: 6124 748 472 418 216 156 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3894 3928 6325 6359 1903 Size: 34 1957 34 2397 34 3678 Fragments arranged by size: 3678 2397 1957 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6026 7236 6026 Size: 1210 6924 BclI T'GATC_A Cuts at: 1548 1548 Size: 8134 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4857 5019 6745 Size: 107 122 71 631 605 3185 162 1726 Cuts at: 6745 136 Size: 1525 Fragments arranged by size: 3185 1726 1525 631 605 162 122 107 71 BglII A'GATC_T Cuts at: 8129 8129 Size: 8134 BlpI GC'TnA_GC Cuts at: 2109 4881 5551 2109 Size: 2772 670 4692 Fragments arranged by size: 4692 2772 670 Bme1580I G_kGCm'C Cuts at: 1790 2090 3354 3504 4583 4763 6195 7351 1790 Size: 300 1264 150 1079 180 1432 1156 2573 Fragments arranged by size: 2573 1432 1264 1156 1079 300 180 150 BmgBI CAC'GTC Cuts at: 1318 2757 2809 1318 Size: 1439 52 6643 Fragments arranged by size: 6643 1439 52 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4000 5865 6823 Size: 416 53 83 399 24 2583 1865 958 Cuts at: 6823 442 Size: 1753 Fragments arranged by size: 2583 1865 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8134 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2734 3449 4330 2734 Size: 715 881 6538 Fragments arranged by size: 6538 881 715 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2720 3444 3447 3642 4340 4739 4979 6794 Size: 1481 724 3 195 698 399 240 1815 Cuts at: 6794 1239 Size: 2579 Fragments arranged by size: 2579 1815 1481 724 698 399 240 195 3 Bpu10I CC'TnA_GC Cuts at: 2131 2147 2283 2549 3297 3453 3669 4141 2131 Size: 16 136 266 748 156 216 472 6124 Fragments arranged by size: 6124 748 472 266 216 156 136 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2870 4217 4502 6259 7124 7422 2870 Size: 1347 285 1757 865 298 3582 Fragments arranged by size: 3582 1757 1347 865 298 285 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2722 4104 6797 882 Size: 617 1223 1382 2693 2219 Fragments arranged by size: 2693 2219 1382 1223 617 BsaAI yAC'GTr Cuts at: 493 1204 2794 4578 5925 493 Size: 711 1590 1784 1347 2702 Fragments arranged by size: 2702 1784 1590 1347 711 BsaBI GATnn'nnATC Cuts at: 8128 8128 Size: 8134 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3234 5941 6323 6958 Size: 53 83 186 936 1701 2707 382 635 Cuts at: 6958 275 Size: 1451 Fragments arranged by size: 2707 1701 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2981 3366 4889 5391 6567 6965 7239 7386 7797 Size: 385 1523 502 1176 398 274 147 411 Cuts at: 7797 2981 Size: 3318 Fragments arranged by size: 3318 1523 1176 502 411 398 385 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3986 4016 1178 Size: 30 165 30 2583 30 5296 Fragments arranged by size: 5296 2583 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2570 2984 4129 4607 4790 4949 1186 Size: 1384 414 1145 478 183 159 4371 Fragments arranged by size: 4371 1384 1145 478 414 183 159 BseYI C'CCAG_C Cuts at: 2089 2114 2813 2960 3292 3664 3717 3746 3767 Size: 25 699 147 332 372 53 29 21 Cuts at: 3767 3887 3946 4895 5017 7337 7833 2089 Size: 120 59 949 122 2320 496 2390 Fragments arranged by size: 2390 2320 949 699 496 372 332 147 122 120 59 53 29 25 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2079 2249 2867 3016 4111 1163 Size: 573 343 170 618 149 1095 5186 Fragments arranged by size: 5186 1095 618 573 343 170 149 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3964 5290 5584 6345 7373 1540 Size: 275 186 1963 1326 294 761 1028 2301 Fragments arranged by size: 2301 1963 1326 1028 761 294 275 186 BsiHKAI G_wGCw'C Cuts at: 2216 2289 2562 3132 3504 4755 4887 4914 5187 Size: 73 273 570 372 1251 132 27 273 Cuts at: 5187 6195 6280 7351 2216 Size: 1008 85 1071 2999 Fragments arranged by size: 2999 1251 1071 1008 570 372 273 273 132 85 73 27 BsmI GAATG_Cn' Cuts at: 5645 5738 5645 Size: 93 8041 BsmBI CGTCTCn'nnnn_ Cuts at: 4701 4701 Size: 8134 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2115 2216 2289 2466 2562 2706 Size: 399 300 25 101 73 177 96 144 Cuts at: 2706 3132 3354 3504 3578 4027 4334 4583 4755 Size: 426 222 150 74 449 307 249 172 Cuts at: 4755 4763 4887 4914 4941 5015 5187 5313 5453 Size: 8 124 27 27 74 172 126 140 Cuts at: 5453 6195 6280 7351 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 449 426 399 307 300 249 222 177 172 172 150 144 140 126 124 101 96 85 74 74 73 27 27 25 8 BspEI T'CCGG_A Cuts at: 3366 7797 3366 Size: 4431 3703 BspHI T'CATG_A Cuts at: 6023 7753 6023 Size: 1730 6404 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2109 3594 3989 5489 844 Size: 1265 1485 395 1500 3489 Fragments arranged by size: 3489 1500 1485 1265 395 BsrBI CCG'CTC Cuts at: 1394 1823 3003 3727 4079 6021 1394 Size: 429 1180 724 352 1942 3507 Fragments arranged by size: 3507 1942 1180 724 429 352 BsrDI GCAATG_nn' Cuts at: 65 1248 3247 6629 6803 65 Size: 1183 1999 3382 174 1396 Fragments arranged by size: 3382 1999 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2981 4406 6778 6965 1700 Size: 110 1171 1425 2372 187 2869 Fragments arranged by size: 2869 2372 1425 1171 187 110 BsrGI T'GTAC_A Cuts at: 96 2252 96 Size: 2156 5978 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8134 BssSI C'ACGA_G Cuts at: 6194 7206 6194 Size: 1012 7122 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8134 BstBI TT'CG_AA Cuts at: 6938 6938 Size: 8134 BstEII G'GTnAC_C Cuts at: 1184 4615 1184 Size: 3431 4703 BstXI CCAn_nnnn'nTGG Cuts at: 2744 3229 4903 4951 2744 Size: 485 1674 48 5927 Fragments arranged by size: 5927 1674 485 48 BstYI r'GATC_y Cuts at: 1972 2433 3310 3363 3586 4668 5131 5464 5831 Size: 461 877 53 223 1082 463 333 367 Cuts at: 5831 6216 6233 7685 7771 7783 8129 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1082 877 463 461 385 367 346 333 223 86 53 17 12 BstZ17I GTA'TAC Cuts at: 4727 4727 Size: 8134 Bsu36I CC'TnA_GG Cuts at: 2350 4569 2350 Size: 2219 5915 BtgI C'CryG_G Cuts at: 513 1065 2004 2306 2706 3076 3275 3777 3941 Size: 552 939 302 400 370 199 502 164 Cuts at: 3941 4161 513 Size: 220 4486 Fragments arranged by size: 4486 939 552 502 400 370 302 220 199 164 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2757 487 Size: 683 56 1531 5864 Fragments arranged by size: 5864 1531 683 56 BtsI GCAGTG_nn' Cuts at: 2103 2266 2320 3537 3688 3829 4194 4332 5029 Size: 163 54 1217 151 141 365 138 697 Cuts at: 5029 5650 6444 6464 2103 Size: 621 794 20 3773 Fragments arranged by size: 3773 1217 794 697 621 365 163 151 141 138 54 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8134 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3229 3264 529 Size: 35 2665 35 5399 Fragments arranged by size: 5399 2665 35 35 DraI TTT'AAA Cuts at: 5438 5438 Size: 8134 DraIII CAC_nnn'GTG Cuts at: 3349 3349 Size: 8134 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7141 809 Size: 507 5825 1802 Fragments arranged by size: 5825 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2494 2575 2863 Size: 54 1425 50 275 18 664 81 288 Cuts at: 2863 3219 3340 3780 4158 4426 5581 6470 8099 Size: 356 121 440 378 268 1155 889 1629 Cuts at: 8099 8 Size: 43 Fragments arranged by size: 1629 1425 1155 889 664 440 378 356 288 275 268 121 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5581 1537 Size: 275 3769 4090 Fragments arranged by size: 4090 3769 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2028 2651 2902 3963 4321 5279 6064 1353 Size: 675 623 251 1061 358 958 785 3423 Fragments arranged by size: 3423 1061 958 785 675 623 358 251 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6721 7095 7241 387 Size: 1064 138 5132 374 146 1280 Fragments arranged by size: 5132 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2214 3130 4753 4885 2214 Size: 916 1623 132 5463 Fragments arranged by size: 5463 1623 916 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2220 2720 3444 3447 3642 3672 4340 Size: 393 588 500 724 3 195 30 668 Cuts at: 4340 4739 4979 6197 6794 7581 7849 1239 Size: 399 240 1218 597 787 268 1524 Fragments arranged by size: 1524 1218 787 724 668 597 588 500 399 393 268 240 195 30 3 EcoNI CCTnn'n_nnAGG Cuts at: 2552 3462 2552 Size: 910 7224 EcoO109I rG'GnC_Cy Cuts at: 2086 2485 2545 3289 4177 4759 4760 4830 5028 Size: 399 60 744 888 582 1 70 198 Cuts at: 5028 2086 Size: 5192 Fragments arranged by size: 5192 888 744 582 399 198 70 60 1 EcoRI G'AATT_C Cuts at: 4213 5443 5821 4213 Size: 1230 378 6526 Fragments arranged by size: 6526 1230 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2422 4258 2422 Size: 1836 6298 FspI TGC'GCA Cuts at: 6640 6640 Size: 8134 HaeII r_GCGC'y Cuts at: 1678 2195 2677 3518 7281 1678 Size: 517 482 841 3763 2531 Fragments arranged by size: 3763 2531 841 517 482 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3348 3380 3913 3945 3986 Size: 32 135 32 1776 32 533 32 41 Cuts at: 3986 4018 4253 4285 5116 5148 5397 5404 5429 Size: 32 235 32 831 32 249 7 25 Cuts at: 5429 5436 6775 6807 6849 6881 1373 Size: 7 1339 32 42 32 2626 Fragments arranged by size: 2626 1776 1339 831 533 249 235 135 42 41 32 32 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 4420 5478 5724 669 Size: 988 281 2482 1058 246 3079 Fragments arranged by size: 3079 2482 1058 988 281 246 HindIII A'AGCT_T Cuts at: 748 2454 748 Size: 1706 6428 HpaI GTT'AAC Cuts at: 5724 5724 Size: 8134 KpnI G_GTAC'C Cuts at: 2925 5459 2925 Size: 2534 5600 MfeI C'AATT_G Cuts at: 5733 5733 Size: 8134 MluI A'CGCG_T Cuts at: 8010 8010 Size: 8134 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2884 4079 4493 4536 5102 5356 7248 7432 Size: 1579 1195 414 43 566 254 1892 184 Cuts at: 7432 1305 Size: 2007 Fragments arranged by size: 2007 1892 1579 1195 566 414 254 184 43 MscI TGG'CCA Cuts at: 10 64 1489 1832 2496 2865 3221 3342 3782 Size: 54 1425 343 664 369 356 121 440 Cuts at: 3782 4160 4428 10 Size: 378 268 3716 Fragments arranged by size: 3716 1425 664 440 378 369 356 343 268 121 54 MslI CAynn'nnrTG Cuts at: 518 1166 2603 4628 6092 6451 6610 518 Size: 648 1437 2025 1464 359 159 2042 Fragments arranged by size: 2042 2025 1464 1437 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2093 2308 3225 3296 3668 3750 3805 4000 Size: 487 215 917 71 372 82 55 195 Cuts at: 4000 4663 4877 5017 5550 6227 7375 7620 1606 Size: 663 214 140 533 677 1148 245 2120 Fragments arranged by size: 2120 1148 917 677 663 533 487 372 245 215 214 195 140 82 71 55 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6643 Fragments arranged by size: 6643 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8134 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8134 NotI GC'GGCC_GC Cuts at: 5581 5581 Size: 8134 NspI r_CATG'y Cuts at: 1132 1229 3697 5492 7037 1132 Size: 97 2468 1795 1545 2229 Fragments arranged by size: 2468 2229 1795 1545 97 PasI CC'CwG_GG Cuts at: 1348 4172 1348 Size: 2824 5310 PciI A'CATG_T Cuts at: 7033 7033 Size: 8134 PflMI CCAn_nnn'nTGG Cuts at: 1108 3880 4926 1108 Size: 2772 1046 4316 Fragments arranged by size: 4316 2772 1046 PfoI T'CCnGG_A Cuts at: 2697 4223 4356 4377 4443 4671 4688 2697 Size: 1526 133 21 66 228 17 6143 Fragments arranged by size: 6143 1526 228 133 66 21 17 PmeI GTTT'AAAC Cuts at: 5438 5438 Size: 8134 PmlI CAC'GTG Cuts at: 4578 4578 Size: 8134 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6203 6235 7716 7748 6203 Size: 32 1481 32 6589 Fragments arranged by size: 6589 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2485 3289 4830 2485 Size: 804 1541 5789 Fragments arranged by size: 5789 1541 804 PshAI GACnn'nnGTC Cuts at: 1613 2726 1613 Size: 1113 7021 PsiI TTA'TAA Cuts at: 5704 5704 Size: 8134 PspOMI G'GGCC_C Cuts at: 2086 4759 2086 Size: 2673 5461 PstI C_TGCA'G Cuts at: 830 3978 4662 5486 830 Size: 3148 684 824 3478 Fragments arranged by size: 3478 3148 824 684 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8134 PvuII CAG'CTG Cuts at: 2093 3225 3296 3668 3805 4000 2093 Size: 1132 71 372 137 195 6227 Fragments arranged by size: 6227 1132 372 195 137 71 SacI G_AGCT'C Cuts at: 2216 3132 4755 4887 2216 Size: 916 1623 132 5463 Fragments arranged by size: 5463 1623 916 132 SacII CC_GC'GG Cuts at: 2309 2309 Size: 8134 SalI G'TCGA_C Cuts at: 1936 5476 1936 Size: 3540 4594 SapI GCTCTTCn'nnn_ Cuts at: 2902 3963 2902 Size: 1061 7073 SbfI CC_TGCA'GG Cuts at: 5486 5486 Size: 8134 ScaI AGT'ACT Cuts at: 4988 6382 8094 4988 Size: 1394 1712 5028 Fragments arranged by size: 5028 1712 1394 SfcI C'TryA_G Cuts at: 826 1045 3169 3974 4658 5482 6617 7298 7489 Size: 219 2124 805 684 824 1135 681 191 Cuts at: 7489 826 Size: 1471 Fragments arranged by size: 2124 1471 1135 824 805 684 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8134 SgrAI Cr'CCGG_yG Cuts at: 1700 2981 1700 Size: 1281 6853 SmaI CCC'GGG Cuts at: 1872 5461 1872 Size: 3589 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2885 4232 4481 6238 7139 7401 Size: 197 926 942 1347 249 1757 901 262 Cuts at: 7401 820 Size: 1553 Fragments arranged by size: 1757 1553 1347 942 926 901 262 249 197 SnaBI TAC'GTA Cuts at: 493 5925 493 Size: 5432 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8134 SphI G_CATG'C Cuts at: 1132 5492 1132 Size: 4360 3774 SspI AAT'ATT Cuts at: 5 52 2248 6058 5 Size: 47 2196 3810 2081 Fragments arranged by size: 3810 2196 2081 47 StuI AGG'CCT Cuts at: 1517 3909 5376 1517 Size: 2392 1467 4275 Fragments arranged by size: 4275 2392 1467 StyI C'CwwG_G Cuts at: 513 1065 2004 3267 3314 3522 3543 3795 4155 Size: 552 939 1263 47 208 21 252 360 Cuts at: 4155 4320 4423 4429 4598 4944 5224 5573 513 Size: 165 103 6 169 346 280 349 3074 Fragments arranged by size: 3074 1263 939 552 360 349 346 280 252 208 169 165 103 47 21 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3013 3240 3978 4880 5369 6161 6178 Size: 1255 1433 227 738 902 489 792 17 Cuts at: 6178 6331 6516 6961 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1433 1255 902 792 738 489 445 227 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2252 4986 4998 Size: 274 80 33 51 1443 275 2734 12 Cuts at: 4998 6380 8092 96 Size: 1382 1712 138 Fragments arranged by size: 2734 1712 1443 1382 275 274 138 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4532 4564 8094 8126 4532 Size: 32 3530 32 4540 Fragments arranged by size: 4540 3530 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3476 1316 Size: 2160 5974 XbaI T'CTAG_A Cuts at: 3931 5470 3931 Size: 1539 6595 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2410 2489 2646 3802 5014 2410 Size: 79 157 1156 1212 5530 Fragments arranged by size: 5530 1212 1156 157 79 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8134 XmaI C'CCGG_G Cuts at: 1870 5459 1870 Size: 3589 4545 XmnI GAAnn'nnTTC Cuts at: 4050 6263 4050 Size: 2213 5921 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3235 5942 276 Size: 53 83 186 936 1701 2707 2468 Fragments arranged by size: 2707 2468 1701 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI