(Linear) (Six-base) MAPSORT of: KIEE2588.seq Check: 314 from: 1 to: 3429 KIEE2588.orf With 182 enzymes: SgfI * September 18, 2015 17:09 .. AatII G_ACGT'C Cuts at: 0 1232 3429 Size: 1232 2197 AccI GT'mk_AC Cuts at: 0 2721 3429 Size: 2721 708 Acc65I G'GTAC_C Cuts at: 0 916 3429 Size: 916 2513 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 215 1667 3429 Size: 215 1452 1762 Fragments arranged by size: 1762 1452 215 AfeI AGC'GCT Cuts at: 0 188 3429 Size: 188 3241 AflIII A'CryG_T Cuts at: 0 788 2535 2570 3429 Size: 788 1747 35 859 Fragments arranged by size: 1747 859 788 35 AgeI A'CCGG_T Cuts at: 0 976 3429 Size: 976 2453 AhdI GACnn_n'nnGTC Cuts at: 0 2262 3429 Size: 2262 1167 AleI CACnn'nnGTG Cuts at: 0 598 2623 3429 Size: 598 2025 806 Fragments arranged by size: 2025 806 598 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 284 1550 3429 Size: 284 1266 1879 Fragments arranged by size: 1879 1266 284 AlwNI CAG_nnn'CTG Cuts at: 0 170 483 526 640 1512 2921 3429 Size: 170 313 43 114 872 1409 508 Fragments arranged by size: 1409 872 508 313 170 114 43 ApaI G_GGCC'C Cuts at: 0 85 2758 3429 Size: 85 2673 671 Fragments arranged by size: 2673 671 85 ApaLI G'TGCA_C Cuts at: 0 1495 3429 Size: 1495 1934 ApoI r'AATT_y Cuts at: 0 2208 3429 Size: 2208 1221 AseI AT'TA_AT Cuts at: 0 1316 3429 Size: 1316 2113 AvaI C'yCGr_G Cuts at: 0 1349 3429 Size: 1349 2080 BamHI G'GATC_C Cuts at: 0 1305 3126 3429 Size: 1305 1821 303 Fragments arranged by size: 1821 1305 303 BanI G'GyrC_C Cuts at: 0 742 916 2404 2674 3376 3429 Size: 742 174 1488 270 702 53 Fragments arranged by size: 1488 742 702 270 174 53 BanII G_rGCy'C Cuts at: 0 85 110 211 461 701 1127 1573 2022 Size: 85 25 101 250 240 426 446 449 Cuts at: 2022 2329 2750 2758 2882 2936 3010 3308 3429 Size: 307 421 8 124 54 74 298 121 Fragments arranged by size: 449 446 426 421 307 298 250 240 124 121 101 85 74 54 25 8 BbsI GAAGACnn'nnnn_ Cuts at: 0 203 3429 Size: 203 3226 BbvCI CC'TCA_GC Cuts at: 0 126 544 1292 1448 1664 2136 3429 Size: 126 418 748 156 216 472 1293 Fragments arranged by size: 1293 748 472 418 216 156 126 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1889 1923 3429 Size: 1889 34 1506 Fragments arranged by size: 1889 1506 34 BglI GCCn_nnn'nGGC Cuts at: 0 2852 3014 3429 Size: 2852 162 415 Fragments arranged by size: 2852 415 162 BlpI GC'TnA_GC Cuts at: 0 104 2876 3429 Size: 104 2772 553 Fragments arranged by size: 2772 553 104 Bme1580I G_kGCm'C Cuts at: 0 85 1349 1499 2578 2758 3429 Size: 85 1264 150 1079 180 671 Fragments arranged by size: 1264 1079 671 180 150 85 BmgBI CAC'GTC Cuts at: 0 752 804 3429 Size: 752 52 2625 Fragments arranged by size: 2625 752 52 BmrI ACTGGGnnnn_n' Cuts at: 0 1995 3429 Size: 1995 1434 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 729 1444 2325 3429 Size: 729 715 881 1104 Fragments arranged by size: 1104 881 729 715 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 715 1439 1442 1637 2335 2734 2974 3429 Size: 715 724 3 195 698 399 240 455 Fragments arranged by size: 724 715 698 455 399 240 195 3 Bpu10I CC'TnA_GC Cuts at: 0 126 142 278 544 1292 1448 1664 2136 Size: 126 16 136 266 748 156 216 472 Cuts at: 2136 3429 Size: 1293 Fragments arranged by size: 1293 748 472 266 216 156 136 126 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 865 2212 2497 3429 Size: 865 1347 285 932 Fragments arranged by size: 1347 932 865 285 BsaI GGTCTCn'nnnn_ Cuts at: 0 717 2099 3429 Size: 717 1382 1330 Fragments arranged by size: 1382 1330 717 BsaAI yAC'GTr Cuts at: 0 789 2573 3429 Size: 789 1784 856 Fragments arranged by size: 1784 856 789 BsaHI Gr'CG_yC Cuts at: 0 1229 3429 Size: 1229 2200 BsaWI w'CCGG_w Cuts at: 0 976 1361 2884 3386 3429 Size: 976 385 1523 502 43 Fragments arranged by size: 1523 976 502 385 43 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1981 2011 3429 Size: 1981 30 1418 Fragments arranged by size: 1981 1418 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 565 979 2124 2602 2785 2944 3429 Size: 565 414 1145 478 183 159 485 Fragments arranged by size: 1145 565 485 478 414 183 159 BseYI C'CCAG_C Cuts at: 0 84 109 808 955 1287 1659 1712 1741 Size: 84 25 699 147 332 372 53 29 Cuts at: 1741 1762 1882 1941 2890 3012 3429 Size: 21 120 59 949 122 417 Fragments arranged by size: 949 699 417 372 332 147 122 120 84 59 53 29 25 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 74 244 862 1011 2106 3429 Size: 74 170 618 149 1095 1323 Fragments arranged by size: 1323 1095 618 170 149 74 BsiEI CG_ry'CG Cuts at: 0 1959 3285 3429 Size: 1959 1326 144 Fragments arranged by size: 1959 1326 144 BsiHKAI G_wGCw'C Cuts at: 0 211 284 557 1127 1499 2750 2882 2909 Size: 211 73 273 570 372 1251 132 27 Cuts at: 2909 3182 3429 Size: 273 247 Fragments arranged by size: 1251 570 372 273 273 247 211 132 73 27 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2696 3429 Size: 2696 733 Bsp1286I G_dGCh'C Cuts at: 0 85 110 211 284 461 557 701 1127 Size: 85 25 101 73 177 96 144 426 Cuts at: 1127 1349 1499 1573 2022 2329 2578 2750 2758 Size: 222 150 74 449 307 249 172 8 Cuts at: 2758 2882 2909 2936 3010 3182 3308 3429 Size: 124 27 27 74 172 126 121 Fragments arranged by size: 449 426 307 249 222 177 172 172 150 144 126 124 121 101 96 85 74 74 73 27 27 25 8 BspEI T'CCGG_A Cuts at: 0 1361 3429 Size: 1361 2068 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 104 1589 1984 3429 Size: 104 1485 395 1445 Fragments arranged by size: 1485 1445 395 104 BsrBI CCG'CTC Cuts at: 0 998 1722 2074 3429 Size: 998 724 352 1355 Fragments arranged by size: 1355 998 724 352 BsrDI GCAATG_nn' Cuts at: 0 1242 3429 Size: 1242 2187 BsrFI r'CCGG_y Cuts at: 0 976 2401 3429 Size: 976 1425 1028 Fragments arranged by size: 1425 1028 976 BsrGI T'GTAC_A Cuts at: 0 247 3429 Size: 247 3182 BstEII G'GTnAC_C Cuts at: 0 2610 3429 Size: 2610 819 BstXI CCAn_nnnn'nTGG Cuts at: 0 739 1224 2898 2946 3429 Size: 739 485 1674 48 483 Fragments arranged by size: 1674 739 485 483 48 BstYI r'GATC_y Cuts at: 0 428 1305 1358 1581 2663 3126 3429 Size: 428 877 53 223 1082 463 303 Fragments arranged by size: 1082 877 463 428 303 223 53 BstZ17I GTA'TAC Cuts at: 0 2722 3429 Size: 2722 707 Bsu36I CC'TnA_GG Cuts at: 0 345 2564 3429 Size: 345 2219 865 Fragments arranged by size: 2219 865 345 BtgI C'CryG_G Cuts at: 0 301 701 1071 1270 1772 1936 2156 3429 Size: 301 400 370 199 502 164 220 1273 Fragments arranged by size: 1273 502 400 370 301 220 199 164 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 752 3429 Size: 752 2677 BtsI GCAGTG_nn' Cuts at: 0 98 261 315 1532 1683 1824 2189 2327 Size: 98 163 54 1217 151 141 365 138 Cuts at: 2327 3024 3429 Size: 697 405 Fragments arranged by size: 1217 697 405 365 163 151 141 138 98 54 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1224 1259 3429 Size: 1224 35 2170 Fragments arranged by size: 2170 1224 35 DraIII CAC_nnn'GTG Cuts at: 0 1344 3429 Size: 1344 2085 EaeI y'GGCC_r Cuts at: 0 489 570 858 1214 1335 1775 2153 2421 Size: 489 81 288 356 121 440 378 268 Cuts at: 2421 3429 Size: 1008 Fragments arranged by size: 1008 489 440 378 356 288 268 121 81 EarI CTCTTCn'nnn_ Cuts at: 0 23 646 897 1958 2316 3274 3429 Size: 23 623 251 1061 358 958 155 Fragments arranged by size: 1061 958 623 358 251 155 23 EcoICRI GAG'CTC Cuts at: 0 209 1125 2748 2880 3429 Size: 209 916 1623 132 549 Fragments arranged by size: 1623 916 549 209 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 215 715 1439 1442 1637 1667 2335 2734 Size: 215 500 724 3 195 30 668 399 Cuts at: 2734 2974 3429 Size: 240 455 Fragments arranged by size: 724 668 500 455 399 240 215 195 30 3 EcoNI CCTnn'n_nnAGG Cuts at: 0 547 1457 3429 Size: 547 910 1972 Fragments arranged by size: 1972 910 547 EcoO109I rG'GnC_Cy Cuts at: 0 81 480 540 1284 2172 2754 2755 2825 Size: 81 399 60 744 888 582 1 70 Cuts at: 2825 3023 3429 Size: 198 406 Fragments arranged by size: 888 744 582 406 399 198 81 70 60 1 EcoRI G'AATT_C Cuts at: 0 2208 3429 Size: 2208 1221 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 417 2253 3429 Size: 417 1836 1176 Fragments arranged by size: 1836 1176 417 HaeII r_GCGC'y Cuts at: 0 190 672 1513 3429 Size: 190 482 841 1916 Fragments arranged by size: 1916 841 482 190 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1343 1375 1908 1940 1981 2013 2248 2280 Size: 1343 32 533 32 41 32 235 32 Cuts at: 2280 3111 3143 3392 3399 3424 3429 Size: 831 32 249 7 25 5 Fragments arranged by size: 1343 831 533 249 235 41 32 32 32 32 32 25 7 5 HincII GTy'rAC Cuts at: 0 2415 3429 Size: 2415 1014 HindIII A'AGCT_T Cuts at: 0 449 3429 Size: 449 2980 KpnI G_GTAC'C Cuts at: 0 920 3429 Size: 920 2509 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 879 2074 2488 2531 3097 3351 3429 Size: 879 1195 414 43 566 254 78 Fragments arranged by size: 1195 879 566 414 254 78 43 MscI TGG'CCA Cuts at: 0 491 860 1216 1337 1777 2155 2423 3429 Size: 491 369 356 121 440 378 268 1006 Fragments arranged by size: 1006 491 440 378 369 356 268 121 MslI CAynn'nnrTG Cuts at: 0 598 2623 3429 Size: 598 2025 806 Fragments arranged by size: 2025 806 598 MspA1I CmG'CkG Cuts at: 0 88 303 1220 1291 1663 1745 1800 1995 Size: 88 215 917 71 372 82 55 195 Cuts at: 1995 2658 2872 3012 3429 Size: 663 214 140 417 Fragments arranged by size: 917 663 417 372 215 214 195 140 88 82 71 55 NspI r_CATG'y Cuts at: 0 1692 3429 Size: 1692 1737 PasI CC'CwG_GG Cuts at: 0 2167 3429 Size: 2167 1262 PflMI CCAn_nnn'nTGG Cuts at: 0 1875 2921 3429 Size: 1875 1046 508 Fragments arranged by size: 1875 1046 508 PfoI T'CCnGG_A Cuts at: 0 692 2218 2351 2372 2438 2666 2683 3429 Size: 692 1526 133 21 66 228 17 746 Fragments arranged by size: 1526 746 692 228 133 66 21 17 PmlI CAC'GTG Cuts at: 0 2573 3429 Size: 2573 856 PpuMI rG'GwC_Cy Cuts at: 0 480 1284 2825 3429 Size: 480 804 1541 604 Fragments arranged by size: 1541 804 604 480 PshAI GACnn'nnGTC Cuts at: 0 721 3429 Size: 721 2708 PspOMI G'GGCC_C Cuts at: 0 81 2754 3429 Size: 81 2673 675 Fragments arranged by size: 2673 675 81 PstI C_TGCA'G Cuts at: 0 1973 2657 3429 Size: 1973 684 772 Fragments arranged by size: 1973 772 684 PvuII CAG'CTG Cuts at: 0 88 1220 1291 1663 1800 1995 3429 Size: 88 1132 71 372 137 195 1434 Fragments arranged by size: 1434 1132 372 195 137 88 71 SacI G_AGCT'C Cuts at: 0 211 1127 2750 2882 3429 Size: 211 916 1623 132 547 Fragments arranged by size: 1623 916 547 211 132 SacII CC_GC'GG Cuts at: 0 304 3429 Size: 304 3125 SapI GCTCTTCn'nnn_ Cuts at: 0 897 1958 3429 Size: 897 1061 1471 Fragments arranged by size: 1471 1061 897 ScaI AGT'ACT Cuts at: 0 2983 3429 Size: 2983 446 SfcI C'TryA_G Cuts at: 0 1164 1969 2653 3429 Size: 1164 805 684 776 Fragments arranged by size: 1164 805 776 684 SgrAI Cr'CCGG_yG Cuts at: 0 976 3429 Size: 976 2453 SmlI C'TyrA_G Cuts at: 0 880 2227 2476 3429 Size: 880 1347 249 953 Fragments arranged by size: 1347 953 880 249 SspI AAT'ATT Cuts at: 0 243 3429 Size: 243 3186 StuI AGG'CCT Cuts at: 0 1904 3371 3429 Size: 1904 1467 58 Fragments arranged by size: 1904 1467 58 StyI C'CwwG_G Cuts at: 0 1262 1309 1517 1538 1790 2150 2315 2418 Size: 1262 47 208 21 252 360 165 103 Cuts at: 2418 2424 2593 2939 3219 3429 Size: 6 169 346 280 210 Fragments arranged by size: 1262 360 346 280 252 210 208 169 165 103 47 21 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1008 1235 1973 2875 3364 3429 Size: 1008 227 738 902 489 65 Fragments arranged by size: 1008 902 738 489 227 65 TatI w'GTAC_w Cuts at: 0 247 2981 2993 3429 Size: 247 2734 12 436 Fragments arranged by size: 2734 436 247 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2527 2559 3429 Size: 2527 32 870 Fragments arranged by size: 2527 870 32 Tth111I GACn'n_nGTC Cuts at: 0 1471 3429 Size: 1471 1958 XbaI T'CTAG_A Cuts at: 0 1926 3429 Size: 1926 1503 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 405 484 641 1797 3009 3429 Size: 405 79 157 1156 1212 420 Fragments arranged by size: 1212 1156 420 405 157 79 XmnI GAAnn'nnTTC Cuts at: 0 2045 3429 Size: 2045 1384 ZraI GAC'GTC Cuts at: 0 1230 3429 Size: 1230 2199 Enzymes that do cut: AatII AccI Acc65I AcuI AfeI AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI SacII SapI ScaI SfcI SgrAI SmlI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI ZraI Enzymes that do not cut: AarI AclI AflII AloI AscI AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BglII BmtI BsaBI BsiWI BsmI BspHI BssHII BssSI BstAPI BstBI ClaI DraI DrdI EagI EciI EcoRV FseI FspI FspAI HpaI KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI SwaI XhoI XmaI