################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BbeI BbrPI BbvCI BclI BcnI BfaI BfrI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Cac8I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 866 GTAGAC GT^[AC][GT]AC AcsI 967 AAATTC [GA]^AATT[CT] AfaI 127 GTAC GT^AC AocI 1055 CCTTAGG CC^T[ACGT]AGG ApoI 967 AAATTC [GA]^AATT[CT] BamHI 936 GGATCC G^GATCC BanII 403 GGGCTC G[GA]GC[CT]^C BcgI 556 GCAACACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 35 GTATCC GTATCC(6/5) BglI 597 GCCTCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 94 AGATCT A^GATCT BsaJI 322 CCCTGG C^C[ACGT][ACGT]GG BseRI 222 CTCCTC GAGGAG(10/8) BsrBI 100 GAGCGG CCGCTC(-3/-3) BsrDI 61 GCAATG GCAATG(2/0) BsrFI 169 ACCGGC [GA]^CCGG[CT] BstXI 377 CCATGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1055 CCTTAGG CC^T[ACGT]AGG Cfr10I 169 ACCGGC [GA]^CCGG[CT] Cfr13I 595 GGGCC G^G[ACGT]CC DraII 594 GGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 260 TGGCCG [CT]^GGCC[GA] Eco81I 1055 CCTTAGG CC^T[ACGT]AGG EcoNI 39 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 594 GGGGCCT [GA]G^G[ACGT]CC[CT] HapII 170 CCGG C^CGG HpaII 170 CCGG C^CGG MaeIII 49 GTGAC ^GT[ACGT]AC MspA1I 130 CAGCTG C[AC]G^C[GT]G MspI 170 CCGG C^CGG NdeI 633 CATATG CA^TATG PleI 307 GACTC GAGTC(4/5) PvuII 130 CAGCTG CAG^CTG RsaI 127 GTAC GT^AC Sau96I 595 GGGCC G^G[ACGT]CC SfcI 864 CTGTAG C^T[GA][CT]AG SspI 451 AATATT AAT^ATT Tsp45I 49 GTGAC ^GT[GC]AC Tsp509I 968 AATT ^AATT ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AciI 8 CCGC CCGC(-3/-1) 102 GCGG AlwI 937 GATCC GGATC(4/5) 936 GGATC BanI 104 GGTGCC G^G[CT][GA]CC 318 GGCACC BbsI 17 GAAGAC GAAGAC(2/6) 787 GTCTTC BbvI 327 GCAGC GCAGC(8/12) 916 GCAGC BmyI 105 GTGCCC G[AGT]GC[ACT]^C 403 GGGCTC BpuAI 17 GAAGAC GAAGAC(2/6) 787 GTCTTC BsaI 373 GAGACC GGTCTC(1/5) 486 GGTCTC BsiYI 39 CCTCCCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 587 CCTTAAAGGGG BslI 39 CCTCCCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 587 CCTTAAAGGGG Bsp1286I 105 GTGCCC G[AGT]GC[ACT]^C 403 GGGCTC BsrI 54 ACTGG ACTGG(1/-1) 639 CCAGT BssKI 315 CCTGG ^CC[ACGT]GG 323 CCTGG BstF5I 174 CATCC GGATG(2/0) 213 CATCC BstNI 315 CCTGG CC^[AT]GG 323 CCTGG BstYI 94 AGATCT [GA]^GATC[CT] 936 GGATCC EcoRII 315 CCTGG ^CC[AT]GG 323 CCTGG FokI 174 CATCC GGATG(9/13) 213 CATCC HaeIII 261 GGCC GG^CC 596 GGCC HphI 48 GGTGA GGTGA(8/7) 360 GGTGA MflI 94 AGATCT [GA]^GATC[CT] 936 GGATCC MseI 589 TTAA T^TAA 943 TTAA MvaI 315 CCTGG CC^[AT]GG 323 CCTGG ScrFI 315 CCTGG CC^[ACGT]GG 323 CCTGG SmlI 427 CTCAAG C^T[CT][GA]AG 540 CTTGAG TfiI 422 GATTC G^A[AT]TC 709 GATTC Tru9I 589 TTAA T^TAA 943 TTAA TseI 327 GCAGC G^C[AT]GC 916 GCAGC XhoII 94 AGATCT [GA]^GATC[CT] 936 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI BsmAI DdeI DpnII Fnu4HI HinfI ItaI MboI MboII MnlI MslI MwoI NdeII NlaIII NlaIV Sau3AI SfaNI TspRI