Restriction map for
pFN21AE2796
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbsI BbvCI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaHI
BseRI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MscI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 290 TCCGGA T^CCGGA
AlwNI 385 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 397 CCCGAG C^[CT]CG[GA]G
AvaII 1206 GGACC G^G[AT]CC
BanII 85 GGGCTC G[GA]GC[CT]^C
BbrPI 562 CACGTG CAC^GTG
BcgI 995 GCATCCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 630 GTATCC GTATCC(6/5)
BclI 1055 TGATCA T^GATCA
BcnI 543 CCCGG CC^[GC]GG
BglI 16 GCCGCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 562 CACGTG [CT]AC^GT[GA]
BsaBI 1056 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 950 GAGACC GGTCTC(1/5)
BsaWI 290 TCCGGA [AT]^CCGG[AT]
BseAI 290 TCCGGA T^CCGGA
BsiEI 1094 CGACCG CG[GA][CT]^CG
BsoBI 397 CCCGAG C^[CT]CG[GA]G
BspEI 290 TCCGGA T^CCGGA
BsrI 821 ACTGG ACTGG(1/-1)
DdeI 326 CTCAG C^T[ACGT]AG
DraI 582 TTTAAA TTT^AAA
DraII 386 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 417 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 785 TGGCCG [CT]^GGCC[GA]
EarI 343 CTCTTC CTCTTC(1/4)
EcoO109I 386 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 245 CCAAGG C^C[AT][AT]GG
FauI 4 GCGGG CCCGC(4/6)
FbaI 1055 TGATCA T^GATCA
HincII 817 GTCAAC GT[CT]^[GA]AC
HindII 817 GTCAAC GT[CT]^[GA]AC
Ksp632I 343 CTCTTC CTCTTC(1/4)
MamI 1056 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 290 TCCGGA T^CCGGA
MspA1I 438 CAGCTG C[AC]G^C[GT]G
NciI 543 CCCGG CC^[GC]GG
PflMI 777 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 562 CACGTG CAC^GTG
PmlI 562 CACGTG CAC^GTG
PshAI 243 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 590 CTGCAG CTGCA^G
PvuII 438 CAGCTG CAG^CTG
ScaI 941 AGTACT AGT^ACT
SmlI 688 CTCAAG C^T[CT][GA]AG
StuI 986 AGGCCT AGG^CCT
StyI 245 CCAAGG C^C[AT][AT]GG
Van91I 777 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 637 AAATTT [GA]^AATT[CT]
1033 AAATTT
AflIII 520 ACGTGT A^C[GA][CT]GT
561 ACACGT
ApoI 637 AAATTT [GA]^AATT[CT]
1033 AAATTT
AspHI 61 GTGCTC G[AT]GC[AT]^C
901 GAGCAC
BanI 460 GGCACC G^G[CT][GA]CC
539 GGTGCC
BsiHKAI 61 GTGCTC G[AT]GC[AT]^C
901 GAGCAC
BsmAI 950 GAGAC GTCTC(1/5)
478 GTCTC
BspHI 89 TCATGA T^CATGA
350 TCATGA
BspMI 573 ACCTGC ACCTGC(4/8)
488 GCAGGT
BsrFI 448 GCCGGC [GA]^CCGG[CT]
457 GCCGGC
BstYI 293 GGATCT [GA]^GATC[CT]
717 AGATCC
Cfr10I 448 GCCGGC [GA]^CCGG[CT]
457 GCCGGC
Cfr13I 387 GGGCC G^G[ACGT]CC
1206 GGACC
DrdI 220 GACTCCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1108 GACATCATAGTC
DsaI 224 CCGTGG C^C[GA][CT]GG
788 CCGTGG
HphI 368 TCACC GGTGA(8/7)
674 TCACC
MflI 293 GGATCT [GA]^GATC[CT]
717 AGATCC
NaeI 448 GCCGGC GCC^GGC
457 GCCGGC
NgoMIV 448 GCCGGC G^CCGGC
457 GCCGGC
NlaIV 460 GGCACC GG[ACGT]^[ACGT]CC
539 GGTGCC
RcaI 89 TCATGA T^CATGA
350 TCATGA
Sau96I 387 GGGCC G^G[ACGT]CC
1206 GGACC
SfcI 590 CTGCAG C^T[GA][CT]AG
945 CTACAG
TfiI 287 GATTC G^A[AT]TC
402 GATTC
Tsp45I 265 GTGAC ^GT[GC]AC
1106 GTGAC
TspRI 417 CACTG CA[GC]TG[ACGT][ACGT]^
1079 CACTG
XhoII 293 GGATCT [GA]^GATC[CT]
717 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BbvI BfaI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MvnI MwoI NdeII NlaIII PleI
RsaI Sau3AI ScrFI SfaNI TaqI
Tru9I TseI Tsp509I TthHB8I