(Circular) (Six-base) MAPSORT of: pFN21AE2796.seq Check: 2350 from: 1 to: 5917 pFN21AE2796 5917 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2484 2484 Size: 5917 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3727 278 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3260 1937 Size: 1323 4594 Acc65I G'GTAC_C Cuts at: 3238 3238 Size: 5917 AclI AA'CG_TT Cuts at: 1563 4044 4417 1563 Size: 2481 373 3063 Fragments arranged by size: 3063 2481 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3980 5364 5632 1632 Size: 2348 1384 268 1917 Fragments arranged by size: 2348 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5720 AflIII A'CryG_T Cuts at: 1203 2525 2566 4816 5793 1203 Size: 1322 41 2250 977 1327 Fragments arranged by size: 2250 1327 1322 977 41 AgeI A'CCGG_T Cuts at: 4748 4748 Size: 5917 AhdI GACnn_n'nnGTC Cuts at: 4646 4646 Size: 5917 AleI CACnn'nnGTG Cuts at: 1166 2567 1166 Size: 1401 4516 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3213 1184 Size: 2029 3888 AlwNI CAG_nnn'CTG Cuts at: 2395 3660 5232 2395 Size: 1265 1572 3080 Fragments arranged by size: 3080 1572 1265 ApaLI G'TGCA_C Cuts at: 3974 5130 3974 Size: 1156 4761 ApoI r'AATT_y Cuts at: 1493 2642 3038 3226 3452 3604 5713 1493 Size: 1149 396 188 226 152 2109 1697 Fragments arranged by size: 2109 1697 1149 396 226 188 152 AseI AT'TA_AT Cuts at: 160 1032 4471 160 Size: 872 3439 1606 Fragments arranged by size: 3439 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 2402 3242 1870 Size: 73 459 840 4545 Fragments arranged by size: 4545 840 459 73 BamHI G'GATC_C Cuts at: 3247 3614 3247 Size: 367 5550 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2465 2544 3238 4693 618 Size: 325 217 627 678 79 694 1455 1842 Fragments arranged by size: 1842 1455 694 678 627 325 217 79 BanII G_rGCy'C Cuts at: 1391 2094 3236 1391 Size: 703 1142 4072 Fragments arranged by size: 4072 1142 703 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4946 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2989 3023 4108 4142 1903 Size: 34 1052 34 1085 34 3678 Fragments arranged by size: 3678 1085 1052 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2646 3809 5019 2646 Size: 1163 1210 3544 Fragments arranged by size: 3544 1210 1163 BclI T'GATC_A Cuts at: 1548 3060 1548 Size: 1512 4405 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2027 4528 136 Size: 107 122 71 631 605 355 2501 1525 Fragments arranged by size: 2501 1525 631 605 355 122 107 71 BglII A'GATC_T Cuts at: 5912 5912 Size: 5917 BlpI GC'TnA_GC Cuts at: 3334 3334 Size: 5917 Bme1580I G_kGCm'C Cuts at: 1790 2402 2549 3978 5134 1790 Size: 612 147 1429 1156 2573 Fragments arranged by size: 2573 1429 1156 612 147 BmgBI CAC'GTC Cuts at: 1318 2526 1318 Size: 1208 4709 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2836 3648 4606 Size: 416 53 83 399 24 1419 812 958 Cuts at: 4606 442 Size: 1753 Fragments arranged by size: 1753 1419 958 812 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2359 2359 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4577 1239 Size: 3338 2579 Bpu10I CC'TnA_GC Cuts at: 2331 2331 Size: 5917 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2678 4042 4907 5205 2678 Size: 1364 865 298 3390 Fragments arranged by size: 3390 1364 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2949 4580 882 Size: 617 1450 1631 2219 Fragments arranged by size: 2219 1631 1450 617 BsaAI yAC'GTr Cuts at: 493 1204 2569 3708 493 Size: 711 1365 1139 2702 Fragments arranged by size: 2702 1365 1139 711 BsaBI GATnn'nnATC Cuts at: 3065 5911 3065 Size: 2846 3071 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3724 4106 4741 275 Size: 53 83 186 936 2191 382 635 1451 Fragments arranged by size: 2191 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2295 4350 4748 5022 5169 5580 2295 Size: 2055 398 274 147 411 2632 Fragments arranged by size: 2632 2055 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5692 Fragments arranged by size: 5692 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5917 BseYI C'CCAG_C Cuts at: 5120 5616 5120 Size: 496 5421 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5344 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3102 3367 4128 5156 1540 Size: 275 186 1101 265 761 1028 2301 Fragments arranged by size: 2301 1101 1028 761 275 265 186 BsiHKAI G_wGCw'C Cuts at: 2070 2910 3978 4063 5134 2070 Size: 840 1068 85 1071 2853 Fragments arranged by size: 2853 1071 1068 840 85 BsmI GAATG_Cn' Cuts at: 3428 3521 3428 Size: 93 5824 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2094 2402 2549 2910 3236 3978 Size: 399 280 24 308 147 361 326 742 Cuts at: 3978 4063 5134 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 361 326 308 280 147 85 24 BspEI T'CCGG_A Cuts at: 2295 5580 2295 Size: 3285 2632 BspHI T'CATG_A Cuts at: 2094 2355 3806 5536 2094 Size: 261 1451 1730 2475 Fragments arranged by size: 2475 1730 1451 261 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2484 2587 3272 844 Size: 1640 103 685 3489 Fragments arranged by size: 3489 1640 685 103 BsrBI CCG'CTC Cuts at: 1394 1823 3804 1394 Size: 429 1981 3507 Fragments arranged by size: 3507 1981 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4412 4586 65 Size: 1183 3164 174 1396 Fragments arranged by size: 3164 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2453 2462 4561 4748 1700 Size: 110 643 9 2099 187 2869 Fragments arranged by size: 2869 2099 643 187 110 9 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5917 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5917 BssSI C'ACGA_G Cuts at: 3977 4989 3977 Size: 1012 4905 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5917 BstBI TT'CG_AA Cuts at: 4721 4721 Size: 5917 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5917 BstYI r'GATC_y Cuts at: 1972 2298 2722 3247 3614 3999 4016 5468 5554 Size: 326 424 525 367 385 17 1452 86 Cuts at: 5554 5566 5912 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 525 424 385 367 346 326 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2229 2793 513 Size: 552 939 225 564 3637 Fragments arranged by size: 3637 939 564 552 225 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5178 Fragments arranged by size: 5178 683 56 BtsI GCAGTG_nn' Cuts at: 3083 3433 4227 4247 3083 Size: 350 794 20 4753 Fragments arranged by size: 4753 794 350 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5882 DraI TTT'AAA Cuts at: 2589 3221 2589 Size: 632 5285 DraIII CAC_nnn'GTG Cuts at: 2427 2427 Size: 5917 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2231 3119 4924 809 Size: 507 915 888 1805 1802 Fragments arranged by size: 1805 1802 915 888 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2790 3364 4253 Size: 54 1425 50 275 18 960 574 889 Cuts at: 4253 5882 8 Size: 1629 43 Fragments arranged by size: 1629 1425 960 889 574 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3364 1537 Size: 275 1552 4090 Fragments arranged by size: 4090 1552 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2354 3847 1353 Size: 1001 1493 3423 Fragments arranged by size: 3423 1493 1001 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2327 2870 4504 4878 5024 387 Size: 1064 138 738 543 1634 374 146 1280 Fragments arranged by size: 1634 1280 1064 738 543 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3980 4577 5364 5632 1239 Size: 393 2348 597 787 268 1524 Fragments arranged by size: 2348 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2392 2392 Size: 5917 EcoRI G'AATT_C Cuts at: 3226 3604 3226 Size: 378 5539 FspI TGC'GCA Cuts at: 4423 4423 Size: 5917 HaeII r_GCGC'y Cuts at: 1678 5064 1678 Size: 3386 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2283 2314 2315 2346 4558 Size: 32 135 32 711 31 1 31 2212 Cuts at: 4558 4590 4632 4664 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2212 711 135 42 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 2824 3261 3507 669 Size: 988 281 886 437 246 3079 Fragments arranged by size: 3079 988 886 437 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5917 HpaI GTT'AAC Cuts at: 3507 3507 Size: 5917 KpnI G_GTAC'C Cuts at: 3242 3242 Size: 5917 MfeI C'AATT_G Cuts at: 3516 3516 Size: 5917 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5031 5215 1305 Size: 3726 184 2007 Fragments arranged by size: 3726 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4095 Fragments arranged by size: 4095 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2567 2792 3017 3875 4234 4393 518 Size: 648 1401 225 225 858 359 159 2042 Fragments arranged by size: 2042 1401 858 648 359 225 225 159 MspA1I CmG'CkG Cuts at: 1606 2445 3333 4010 5158 5403 1606 Size: 839 888 677 1148 245 2120 Fragments arranged by size: 2120 1148 888 839 677 245 NaeI GCC'GGC Cuts at: 2455 2464 2455 Size: 9 5908 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4426 Fragments arranged by size: 4426 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NgoMIV G'CCGG_C Cuts at: 2453 2462 2453 Size: 9 5908 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 3364 3364 Size: 5917 NspI r_CATG'y Cuts at: 1132 1229 3275 4820 1132 Size: 97 2046 1545 2229 Fragments arranged by size: 2229 2046 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5917 PciI A'CATG_T Cuts at: 4816 4816 Size: 5917 PflMI CCAn_nnn'nTGG Cuts at: 1108 2788 1108 Size: 1680 4237 PfoI T'CCnGG_A Cuts at: 2781 2781 Size: 5917 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PmlI CAC'GTG Cuts at: 2569 2569 Size: 5917 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3986 4018 5499 5531 3986 Size: 32 1481 32 4372 Fragments arranged by size: 4372 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2252 1613 Size: 639 5278 PsiI TTA'TAA Cuts at: 3487 3487 Size: 5917 PstI C_TGCA'G Cuts at: 830 2599 3269 830 Size: 1769 670 3478 Fragments arranged by size: 3478 1769 670 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5917 PvuII CAG'CTG Cuts at: 2445 2445 Size: 5917 SalI G'TCGA_C Cuts at: 1936 3259 1936 Size: 1323 4594 SbfI CC_TGCA'GG Cuts at: 3269 3269 Size: 5917 ScaI AGT'ACT Cuts at: 2948 4165 5877 2948 Size: 1217 1712 2988 Fragments arranged by size: 2988 1712 1217 SexAI A'CCwGG_T Cuts at: 3213 3213 Size: 5917 SfcI C'TryA_G Cuts at: 826 1045 2595 2950 3265 4400 5081 5272 826 Size: 219 1550 355 315 1135 681 191 1471 Fragments arranged by size: 1550 1471 1135 681 355 315 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5917 SmaI CCC'GGG Cuts at: 1872 3244 1872 Size: 1372 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2693 4021 4922 5184 820 Size: 197 926 750 1328 901 262 1553 Fragments arranged by size: 1553 1328 926 901 750 262 197 SnaBI TAC'GTA Cuts at: 493 3708 493 Size: 3215 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5917 SphI G_CATG'C Cuts at: 1132 3275 1132 Size: 2143 3774 SspI AAT'ATT Cuts at: 5 52 3841 5 Size: 47 3789 2081 Fragments arranged by size: 3789 2081 47 StuI AGG'CCT Cuts at: 1517 2993 1517 Size: 1476 4441 StyI C'CwwG_G Cuts at: 513 1065 2004 2250 3356 513 Size: 552 939 246 1106 3074 Fragments arranged by size: 3074 1106 939 552 246 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2179 2483 3944 3961 4114 4299 4744 Size: 1255 599 304 1461 17 153 185 445 Cuts at: 4744 325 Size: 1498 Fragments arranged by size: 1498 1461 1255 599 445 304 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2447 2946 4163 Size: 274 80 33 51 1443 470 499 1217 Cuts at: 4163 5875 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1217 499 470 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5877 5909 5877 Size: 32 5885 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5917 XbaI T'CTAG_A Cuts at: 3253 3253 Size: 5917 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5917 XmaI C'CCGG_G Cuts at: 1870 3242 1870 Size: 1372 4545 XmnI GAAnn'nnTTC Cuts at: 4046 4046 Size: 5917 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3725 276 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI