Restriction map for
pFN21AB6015
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# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AcyI AgeI AhdI
AscI AspEI AspI AvrII BbeI
BcgI BlnI BlpI Bpu1102I BsaHI
BsiWI BsmFI BsrBI BssHII BstBI
BstXI CelII DrdI Eam1105I EcoRI
FseI Hin1I HpaI KasI KspI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmeI
PshAI Psp1406I PvuI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI Sse8387I
SspI SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3441 GGTACC G^GTACC
AccI 5274 GTATAC GT^[AC][GT]AC
AccII 1198 CGCG CG^CG
AocI 1817 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2747 AGCGCT AGC^GCT
ApaI 1568 GGGCCC GGGCC^C
Asp718I 3441 GGTACC G^GTACC
BbrPI 4409 CACGTG CAC^GTG
BciVI 41 GGATAC GTATCC(6/5)
BglI 559 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 3809 GGTCTC GGTCTC(1/5)
BsiEI 2081 CGGCCG CG[GA][CT]^CG
BsmBI 4578 CGTCTC CGTCTC(1/5)
BspDI 3041 ATCGAT AT^CGAT
BspHI 240 TCATGA T^CATGA
Bst1107I 5274 GTATAC GTA^TAC
BstEII 3708 GGTGACC G^GT[ACGT]ACC
BstPI 3708 GGTGACC G^GT[ACGT]ACC
BstUI 1198 CGCG CG^CG
BstZ17I 5274 GTATAC GTA^TAC
Bsu36I 1817 CCTCAGG CC^T[ACGT]AGG
ClaI 3041 ATCGAT AT^CGAT
CpoI 4193 CGGTCCG CG^G[AT]CCG
DraI 820 TTTAAA TTT^AAA
EagI 2081 CGGCCG C^GGCCG
EclXI 2081 CGGCCG C^GGCCG
Eco47III 2747 AGCGCT AGC^GCT
Eco52I 2081 CGGCCG C^GGCCG
Eco81I 1817 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3708 GGTGACC G^GT[ACGT]ACC
HaeII 2747 AGCGCT [GA]GCGC^[CT]
KpnI 3441 GGTACC GGTAC^C
MvnI 1198 CGCG CG^CG
NcoI 1447 CCATGG C^CATGG
PaeR7I 124 CTCGAG C^TCGAG
PflMI 32 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4409 CACGTG CAC^GTG
PmlI 4409 CACGTG CAC^GTG
PpuMI 2951 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 240 TCATGA T^CATGA
RsrII 4193 CGGTCCG CG^G[AT]CCG
SacI 13 GAGCTC GAGCT^C
ScaI 2468 AGTACT AGT^ACT
SnaBI 300 TACGTA TAC^GTA
StuI 929 AGGCCT AGG^CCT
Van91I 32 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 124 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 41 enzymes) #
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AccIII 2312 TCCGGA T^CCGGA
2984 TCCGGA
AflII 3466 CTTAAG C^TTAAG
3804 CTTAAG
AflIII 3452 ACATGT A^C[GA][CT]GT
5045 ACATGT
Alw44I 1604 GTGCAC G^TGCAC
3722 GTGCAC
ApaLI 1604 GTGCAC G^TGCAC
3722 GTGCAC
AseI 370 ATTAAT AT^TAAT
1486 ATTAAT
AsnI 370 ATTAAT AT^TAAT
1486 ATTAAT
AvaI 124 CTCGAG C^[CT]CG[GA]G
1025 CCCGAG
AviII 2435 TGCGCA TGC^GCA
3674 TGCGCA
BamHI 405 GGATCC G^GATCC
2315 GGATCC
BanII 13 GAGCTC G[GA]GC[CT]^C
1568 GGGCCC
BbvCI 228 CCTCAGC CCTCAGC(-5/-2)
800 CCTCAGC
BclI 4494 TGATCA T^GATCA
4970 TGATCA
BcnI 2619 CCGGG CC^[GC]GG
4136 CCCGG
BfrI 3466 CTTAAG C^TTAAG
3804 CTTAAG
BglII 2240 AGATCT A^GATCT
4742 AGATCT
BsaBI 400 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1016 GATTTACATC
BsaWI 2312 TCCGGA [AT]^CCGG[AT]
2984 TCCGGA
BseAI 2312 TCCGGA T^CCGGA
2984 TCCGGA
BsoBI 124 CTCGAG C^[CT]CG[GA]G
1025 CCCGAG
Bsp1407I 1580 TGTACA T^GTACA
3894 TGTACA
BspEI 2312 TCCGGA T^CCGGA
2984 TCCGGA
BspLU11I 3452 ACATGT A^CATGT
5045 ACATGT
BsrDI 1007 GCAATG GCAATG(2/0)
5231 GCAATG
BsrGI 1580 TGTACA T^GTACA
3894 TGTACA
BssSI 4126 CACGAG CACGAG(-5/-1)
4756 CTCGTG
DraIII 1966 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
4409 CACGTGGTG
DsaI 1447 CCATGG C^C[GA][CT]GG
4020 CCGTGG
EcoNI 4038 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5034 CCTGCGGAAGG
EcoRV 692 GATATC GAT^ATC
3230 GATATC
FbaI 4494 TGATCA T^GATCA
4970 TGATCA
FspI 2435 TGCGCA TGC^GCA
3674 TGCGCA
HgaI 7 GCGTC GACGC(5/10)
3621 GCGTC
HincII 4309 GTCAAC GT[CT]^[GA]AC
5090 GTCAAC
HindII 4309 GTCAAC GT[CT]^[GA]AC
5090 GTCAAC
HindIII 434 AAGCTT A^AGCTT
1637 AAGCTT
MamI 400 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1016 GATTTACATC
MroI 2312 TCCGGA T^CCGGA
2984 TCCGGA
NciI 2619 CCGGG CC^[GC]GG
4136 CCCGG
PshBI 370 ATTAAT AT^TAAT
1486 ATTAAT
SspBI 1580 TGTACA T^GTACA
3894 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BanI BbsI BbvI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XcmI XhoII XmnI