Restriction map for
pFN21AB6015
(Circular) (Six-base) MAPSORT of: pFN21AB6015.seq Check: 2895 from: 1 to: 10189
pFN21AB6015 10189 bp
With 182 enzymes: SgfI *
December 17, 2018 11:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3945 5503 5539 5740 6712 6973 3945
Size: 1558 36 201 972 261 7161
Fragments arranged by size:
7161 1558 972 261 201 36
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7999 278
Size: 53 83 186 936 6463 2468
Fragments arranged by size:
6463 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7280 7532 1937
Size: 5343 252 4594
Fragments arranged by size:
5343 4594 252
Acc65I G'GTAC_C
Cuts at: 5446 7510 5446
Size: 2064 8125
AclI AA'CG_TT
Cuts at: 1563 8316 8689 1563
Size: 6753 373 3063
Fragments arranged by size:
6753 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2454 3795 4520 4664 4675 5105 5126 5678
Size: 822 1341 725 144 11 430 21 552
Cuts at: 5678 6065 6428 6770 8252 9636 9904 1632
Size: 387 363 342 1482 1384 268 1917
Fragments arranged by size:
1917 1482 1384 1341 822 725 552 430
387 363 342 268 144 21 11
AfeI AGC'GCT
Cuts at: 4754 4754
Size: 10189
AflII C'TTAA_G
Cuts at: 820 1017 5471 5809 820
Size: 197 4454 338 5200
Fragments arranged by size:
5200 4454 338 197
AflIII A'CryG_T
Cuts at: 1203 5457 7050 9088 10065 1203
Size: 4254 1593 2038 977 1327
Fragments arranged by size:
4254 2038 1593 1327 977
AgeI A'CCGG_T
Cuts at: 9020 9020
Size: 10189
AhdI GACnn_n'nnGTC
Cuts at: 8918 8918
Size: 10189
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10189
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3061 3243 1184
Size: 1877 182 8130
Fragments arranged by size:
8130 1877 182
AlwNI CAG_nnn'CTG
Cuts at: 2432 4045 6043 6073 6076 6088 6727 7932 9504
Size: 1613 1998 30 3 12 639 1205 1572
Cuts at: 9504 2432
Size: 3117
Fragments arranged by size:
3117 1998 1613 1572 1205 639 30 12
3
ApaI G_GGCC'C
Cuts at: 3577 3577
Size: 10189
ApaLI G'TGCA_C
Cuts at: 3609 5727 8246 9402 3609
Size: 2118 2519 1156 4396
Fragments arranged by size:
4396 2519 2118 1156
ApoI r'AATT_y
Cuts at: 1493 2822 2976 3447 4118 7273 7498 7724 7876
Size: 1329 154 471 671 3155 225 226 152
Cuts at: 7876 9985 1493
Size: 2109 1697
Fragments arranged by size:
3155 2109 1697 1329 671 471 226 225
154 152
AseI AT'TA_AT
Cuts at: 160 1032 2376 3492 8743 160
Size: 872 1344 1116 5251 1606
Fragments arranged by size:
5251 1606 1344 1116 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10189
AvaI C'yCGr_G
Cuts at: 1870 1943 2129 3030 7514 1870
Size: 73 186 901 4484 4545
Fragments arranged by size:
4545 4484 901 186 73
BamHI G'GATC_C
Cuts at: 2410 4320 7519 7886 2410
Size: 1910 3199 367 4713
Fragments arranged by size:
4713 3199 1910 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2711 5446 6893 7510 8965
Size: 325 217 627 924 2735 1447 617 1455
Cuts at: 8965 618
Size: 1842
Fragments arranged by size:
2735 1842 1455 1447 924 627 617 325
217
BanII G_rGCy'C
Cuts at: 1391 2022 3577 7508 1391
Size: 631 1555 3931 4072
Fragments arranged by size:
4072 3931 1555 631
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3403 4334 4907 5412 928
Size: 971 1504 931 573 505 5705
Fragments arranged by size:
5705 1504 971 931 573 505
BbvCI CC'TCA_GC
Cuts at: 2234 2806 2234
Size: 572 9617
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8380 8414 1903
Size: 34 6443 34 3678
Fragments arranged by size:
6443 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2040 8081 9291 2040
Size: 6041 1210 2938
Fragments arranged by size:
6041 2938 1210
BclI T'GATC_A
Cuts at: 1548 6499 6975 1548
Size: 4951 476 4762
Fragments arranged by size:
4951 4762 476
BfrBI ATG'CAT
Cuts at: 2381 3406 4663 5028 2381
Size: 1025 1257 365 7542
Fragments arranged by size:
7542 1257 1025 365
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2570 8800 136
Size: 107 122 71 631 605 898 6230 1525
Fragments arranged by size:
6230 1525 898 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4245 6747 10184 4245
Size: 2502 3437 4250
Fragments arranged by size:
4250 3437 2502
BlpI GC'TnA_GC
Cuts at: 7606 7606
Size: 10189
Bme1580I G_kGCm'C
Cuts at: 1790 3577 3613 5707 5731 6896 8250 9406 1790
Size: 1787 36 2094 24 1165 1354 1156 2573
Fragments arranged by size:
2573 2094 1787 1354 1165 1156 36 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10189
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4959 7920 8878
Size: 416 53 83 399 24 3542 2961 958
Cuts at: 8878 442
Size: 1753
Fragments arranged by size:
3542 2961 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10189
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4759 4759
Size: 10189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5021 5462 5519 6859 7081 7269 8849 1239
Size: 3782 441 57 1340 222 188 1580 2579
Fragments arranged by size:
3782 2579 1580 1340 441 222 188 57
Bpu10I CC'TnA_GC
Cuts at: 2234 2806 5151 5322 2234
Size: 572 2345 171 7101
Fragments arranged by size:
7101 2345 572 171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5273 5606 8314 9179 9477 5273
Size: 333 2708 865 298 5985
Fragments arranged by size:
5985 2708 865 333 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5820 8852 882
Size: 617 4321 3032 2219
Fragments arranged by size:
4321 3032 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2307 4402 6416 7980 493
Size: 711 1103 2095 2014 1564 2702
Fragments arranged by size:
2702 2095 2014 1564 1103 711
BsaBI GATnn'nnATC
Cuts at: 2409 3025 10183 2409
Size: 616 7158 2415
Fragments arranged by size:
7158 2415 616
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7996 8378 9013 275
Size: 53 83 186 936 6463 382 635 1451
Fragments arranged by size:
6463 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4317 4989 8622 9020 9294 9441 9852 4317
Size: 672 3633 398 274 147 411 4654
Fragments arranged by size:
4654 3633 672 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2097 2127 3040 3070 3832
Size: 30 165 30 694 30 913 30 762
Cuts at: 3832 3862 5165 5195 7040 7070 1178
Size: 30 1303 30 1845 30 4297
Fragments arranged by size:
4297 1845 1303 913 762 694 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2783 3125 3811 4669 5558 5789 6010 6065
Size: 1597 342 686 858 889 231 221 55
Cuts at: 6065 1186
Size: 5310
Fragments arranged by size:
5310 1597 889 858 686 342 231 221
55
BseYI C'CCAG_C
Cuts at: 5976 7368 9392 9888 5976
Size: 1392 2024 496 6277
Fragments arranged by size:
6277 2024 1392 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2923 3031 3917 4058 4751 5162 5619
Size: 573 1187 108 886 141 693 411 457
Cuts at: 5619 1163
Size: 5733
Fragments arranged by size:
5733 1187 886 693 573 457 411 141
108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4089 7639 8400 9428 1540
Size: 275 186 2088 3550 761 1028 2301
Fragments arranged by size:
3550 2301 2088 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2022 2262 3613 3897 4596 5556 5731 7058 8250
Size: 240 1351 284 699 960 175 1327 1192
Cuts at: 8250 8335 9406 2022
Size: 85 1071 2805
Fragments arranged by size:
2805 1351 1327 1192 1071 960 699 284
240 175 85
BsmI GAATG_Cn'
Cuts at: 3202 3516 3545 7700 7793 3202
Size: 314 29 4155 93 5598
Fragments arranged by size:
5598 4155 314 93 29
BsmBI CGTCTCn'nnnn_
Cuts at: 6589 6589
Size: 10189
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2262 3577 3613 3897 4596 5556
Size: 399 232 240 1315 36 284 699 960
Cuts at: 5556 5707 5731 6896 7058 7508 8250 8335 9406
Size: 151 24 1165 162 450 742 85 1071
Cuts at: 9406 1391
Size: 2174
Fragments arranged by size:
2174 1315 1165 1071 960 742 699 450
399 284 240 232 162 151 85 36
24
BspEI T'CCGG_A
Cuts at: 4317 4989 9852 4317
Size: 672 4863 4654
Fragments arranged by size:
4863 4654 672
BspHI T'CATG_A
Cuts at: 2245 8078 9808 2245
Size: 5833 1730 2626
Fragments arranged by size:
5833 2626 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3945 5503 5539 5740 6096 6712 6973 7544
Size: 3101 1558 36 201 356 616 261 571
Cuts at: 7544 844
Size: 3489
Fragments arranged by size:
3489 3101 1558 616 571 356 261 201
36
BsrBI CCG'CTC
Cuts at: 1394 1823 8076 1394
Size: 429 6253 3507
Fragments arranged by size:
6253 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3019 7243 8684 8858 65
Size: 1183 1771 4224 1441 174 1396
Fragments arranged by size:
4224 1771 1441 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4088 4259 6448 8833 9020 1700
Size: 110 2278 171 2189 2385 187 2869
Fragments arranged by size:
2869 2385 2278 2189 187 171 110
BsrGI T'GTAC_A
Cuts at: 96 3585 5899 96
Size: 3489 2314 4386
Fragments arranged by size:
4386 3489 2314
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10189
BssSI C'ACGA_G
Cuts at: 6131 6761 8249 9261 6131
Size: 630 1488 1012 7059
Fragments arranged by size:
7059 1488 1012 630
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5518 6073 6763 794
Size: 4724 555 690 4220
Fragments arranged by size:
4724 4220 690 555
BstBI TT'CG_AA
Cuts at: 8993 8993
Size: 10189
BstEII G'GTnAC_C
Cuts at: 1184 5713 1184
Size: 4529 5660
BstYI r'GATC_y
Cuts at: 1972 2161 2410 4245 4320 6033 6747 7519 7886
Size: 189 249 1835 75 1713 714 772 367
Cuts at: 7886 8271 8288 9740 9826 9838 10184 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1835 1713 1452 772 714 385 367
346 249 189 86 75 17 12
BstZ17I GTA'TAC
Cuts at: 7281 7281
Size: 10189
Bsu36I CC'TnA_GG
Cuts at: 3823 3823
Size: 10189
BtgI C'CryG_G
Cuts at: 513 1065 2004 3452 6025 513
Size: 552 939 1448 2573 4677
Fragments arranged by size:
4677 2573 1448 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3219 487
Size: 683 56 1993 7457
Fragments arranged by size:
7457 1993 683 56
BtsI GCAGTG_nn'
Cuts at: 4041 6619 6685 7705 8499 8519 4041
Size: 2578 66 1020 794 20 5711
Fragments arranged by size:
5711 2578 1020 794 66 20
ClaI AT'CG_AT
Cuts at: 1554 5047 1554
Size: 3493 6696
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10154
DraI TTT'AAA
Cuts at: 2827 7493 2827
Size: 4666 5523
DraIII CAC_nnn'GTG
Cuts at: 3976 6419 3976
Size: 2443 7746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9196 809
Size: 507 7880 1802
Fragments arranged by size:
7880 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2454 3213 3885
Size: 54 1425 50 275 18 624 759 672
Cuts at: 3885 4086 4560 4704 6738 7636 8525 10154 8
Size: 201 474 144 2034 898 889 1629 43
Fragments arranged by size:
2034 1629 1425 898 889 759 672 624
474 275 201 144 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4086 7636 1537
Size: 275 2274 3550 4090
Fragments arranged by size:
4090 3550 2274 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2760 4022 4923 5289 5987 6639 8119 1353
Size: 1407 1262 901 366 698 652 1480 3423
Fragments arranged by size:
3423 1480 1407 1262 901 698 652 366
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4711 8776 9150 9296 387
Size: 1064 138 3122 4065 374 146 1280
Fragments arranged by size:
4065 3122 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2020 2020
Size: 10189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2454 3795 4520 4664 4675 5021 5105
Size: 393 822 1341 725 144 11 346 84
Cuts at: 5105 5126 5462 5519 5678 6065 6428 6770 6859
Size: 21 336 57 159 387 363 342 89
Cuts at: 6859 7081 7269 8252 8849 9636 9904 1239
Size: 222 188 983 597 787 268 1524
Fragments arranged by size:
1524 1341 983 822 787 725 597 393
387 363 346 342 336 268 222 188
159 144 89 84 57 21 11
EcoNI CCTnn'n_nnAGG
Cuts at: 6047 7043 6047
Size: 996 9193
EcoO109I rG'GnC_Cy
Cuts at: 4957 5686 6019 4957
Size: 729 333 9127
Fragments arranged by size:
9127 729 333
EcoRI G'AATT_C
Cuts at: 7498 7876 7498
Size: 378 9811
EcoRV GAT'ATC
Cuts at: 2699 5237 2699
Size: 2538 7651
FspI TGC'GCA
Cuts at: 4442 5681 8695 4442
Size: 1239 3014 5936
Fragments arranged by size:
5936 3014 1239
HaeII r_GCGC'y
Cuts at: 1678 4756 9336 1678
Size: 3078 4580 2531
Fragments arranged by size:
4580 3078 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2038 2070 2097 2129 2688
Size: 32 135 32 466 32 27 32 559
Cuts at: 2688 2713 2720 2745 2764 2796 5399 5431 6260
Size: 25 7 25 19 32 2603 32 829
Cuts at: 6260 6292 7040 7072 8830 8862 8904 8936 1373
Size: 32 748 32 1758 32 42 32 2626
Fragments arranged by size:
2626 2603 1758 829 748 559 466 135
42 32 32 32 32 32 32 32
32 32 32 27 25 25 19 7
HincII GTy'rAC
Cuts at: 669 1657 1938 6316 7097 7533 7779 669
Size: 988 281 4378 781 436 246 3079
Fragments arranged by size:
4378 3079 988 781 436 281 246
HindIII A'AGCT_T
Cuts at: 748 2439 3642 748
Size: 1691 1203 7295
Fragments arranged by size:
7295 1691 1203
HpaI GTT'AAC
Cuts at: 7779 7779
Size: 10189
KpnI G_GTAC'C
Cuts at: 5450 7514 5450
Size: 2064 8125
MfeI C'AATT_G
Cuts at: 7788 7788
Size: 10189
MluI A'CGCG_T
Cuts at: 10065 10065
Size: 10189
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1995 2625 2998 3679 3830 5869 6331 9303
Size: 690 630 373 681 151 2039 462 2972
Cuts at: 9303 9487 1305
Size: 184 2007
Fragments arranged by size:
2972 2039 2007 690 681 630 462 373
184 151
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2456 3215 3887 4562 4706
Size: 54 1425 343 624 759 672 675 144
Cuts at: 4706 6740 10
Size: 2034 3459
Fragments arranged by size:
3459 2034 1425 759 675 672 624 343
144 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3098 3225 4388 4487 4775 6006 8147
Size: 648 1932 127 1163 99 288 1231 2141
Cuts at: 8147 8506 8665 518
Size: 359 159 2042
Fragments arranged by size:
2141 2042 1932 1231 1163 648 359 288
159 127 99
MspA1I CmG'CkG
Cuts at: 1606 2350 3045 4045 4798 5071 5758 5980 6073
Size: 744 695 1000 753 273 687 222 93
Cuts at: 6073 6094 7372 7605 8282 9430 9675 1606
Size: 21 1278 233 677 1148 245 2120
Fragments arranged by size:
2120 1278 1148 1000 753 744 695 687
677 273 245 233 222 93 21
NcoI C'CATG_G
Cuts at: 513 1065 2004 3452 513
Size: 552 939 1448 7250
Fragments arranged by size:
7250 1448 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10189
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10189
NotI GC'GGCC_GC
Cuts at: 7636 7636
Size: 10189
NsiI A_TGCA'T
Cuts at: 2383 3408 4665 5030 2383
Size: 1025 1257 365 7542
Fragments arranged by size:
7542 1257 1025 365
NspI r_CATG'y
Cuts at: 1132 1229 3410 4056 4185 4372 4486 4663 4667
Size: 97 2181 646 129 187 114 177 4
Cuts at: 4667 5042 5318 5461 6482 6609 6811 7054 7075
Size: 375 276 143 1021 127 202 243 21
Cuts at: 7075 7547 9092 1132
Size: 472 1545 2229
Fragments arranged by size:
2229 2181 1545 1021 646 472 375 276
243 202 187 177 143 129 127 114
97 21 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10189
PciI A'CATG_T
Cuts at: 5457 7050 9088 5457
Size: 1593 2038 6558
Fragments arranged by size:
6558 2038 1593
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2043 1108
Size: 935 9254
PfoI T'CCnGG_A
Cuts at: 4062 5967 6439 7058 4062
Size: 1905 472 619 7193
Fragments arranged by size:
7193 1905 619 472
PmeI GTTT'AAAC
Cuts at: 7493 7493
Size: 10189
PmlI CAC'GTG
Cuts at: 6416 6416
Size: 10189
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6675 6707 8258 8290 9771 9803 6675
Size: 32 1551 32 1481 32 7061
Fragments arranged by size:
7061 1551 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4957 4957
Size: 10189
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10189
PsiI TTA'TAA
Cuts at: 2097 7759 2097
Size: 5662 4527
PspOMI G'GGCC_C
Cuts at: 3573 3573
Size: 10189
PspXI vC'TCGA_Gb
Cuts at: 2129 2129
Size: 10189
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4814 4846 4814
Size: 32 10157
PstI C_TGCA'G
Cuts at: 830 3189 4803 4971 5985 6093 6129 7541 830
Size: 2359 1614 168 1014 108 36 1412 3478
Fragments arranged by size:
3478 2359 1614 1412 1014 168 108 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10189
PvuII CAG'CTG
Cuts at: 2350 3045 4045 4798 5071 5758 5980 6073 6094
Size: 695 1000 753 273 687 222 93 21
Cuts at: 6094 7372 2350
Size: 1278 5167
Fragments arranged by size:
5167 1278 1000 753 695 687 273 222
93 21
RsrII CG'GwC_CG
Cuts at: 6199 6199
Size: 10189
SacI G_AGCT'C
Cuts at: 2022 2022
Size: 10189
SalI G'TCGA_C
Cuts at: 1936 7531 1936
Size: 5595 4594
SbfI CC_TGCA'GG
Cuts at: 7541 7541
Size: 10189
ScaI AGT'ACT
Cuts at: 4475 8437 10149 4475
Size: 3962 1712 4515
Fragments arranged by size:
4515 3962 1712
SfcI C'TryA_G
Cuts at: 826 1045 3185 3386 4799 4967 5981 6089 6125
Size: 219 2140 201 1413 168 1014 108 36
Cuts at: 6125 7537 8672 9353 9544 826
Size: 1412 1135 681 191 1471
Fragments arranged by size:
2140 1471 1413 1412 1135 1014 681 219
201 191 168 108 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10189
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10189
SmaI CCC'GGG
Cuts at: 1872 7516 1872
Size: 5644 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2129 5288 5471 5621 5809 8293
Size: 197 926 186 3159 183 150 188 2484
Cuts at: 8293 9194 9456 820
Size: 901 262 1553
Fragments arranged by size:
3159 2484 1553 926 901 262 197 188
186 183 150
SnaBI TAC'GTA
Cuts at: 493 2307 7980 493
Size: 1814 5673 2702
Fragments arranged by size:
5673 2702 1814
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10189
SphI G_CATG'C
Cuts at: 1132 4372 4663 4667 6811 7075 7547 1132
Size: 3240 291 4 2144 264 472 3774
Fragments arranged by size:
3774 3240 2144 472 291 264 4
SspI AAT'ATT
Cuts at: 5 52 8113 5
Size: 47 8061 2081
Fragments arranged by size:
8061 2081 47
StuI AGG'CCT
Cuts at: 1517 2936 1517
Size: 1419 8770
StyI C'CwwG_G
Cuts at: 513 1065 2004 2172 3452 4449 5568 5850 6343
Size: 552 939 168 1280 997 1119 282 493
Cuts at: 6343 7628 513
Size: 1285 3074
Fragments arranged by size:
3074 1285 1280 1119 997 939 552 493
282 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2390 3086 5366 6931 8216 8233 8386
Size: 1255 810 696 2280 1565 1285 17 153
Cuts at: 8386 8571 9016 325
Size: 185 445 1498
Fragments arranged by size:
2280 1565 1498 1285 1255 810 696 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3585 4473 5899
Size: 274 80 33 51 1443 1608 888 1426
Cuts at: 5899 6633 8435 10147 96
Size: 734 1802 1712 138
Fragments arranged by size:
1802 1712 1608 1443 1426 888 734 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3040 3072 3668 3700 3832 3864 10149 10181 3040
Size: 32 596 32 132 32 6285 32 3048
Fragments arranged by size:
6285 3048 596 132 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10189
XbaI T'CTAG_A
Cuts at: 2158 3520 4276 7525 2158
Size: 1362 756 3249 4822
Fragments arranged by size:
4822 3249 1362 756
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2064 2608 5887 6157 6703 7465 2064
Size: 544 3279 270 546 762 4788
Fragments arranged by size:
4788 3279 762 546 544 270
XhoI C'TCGA_G
Cuts at: 1943 2129 1943
Size: 186 10003
XmaI C'CCGG_G
Cuts at: 1870 7514 1870
Size: 5644 4545
XmnI GAAnn'nnTTC
Cuts at: 3447 3693 3777 4914 5255 5409 6825 8318 3447
Size: 246 84 1137 341 154 1416 1493 5318
Fragments arranged by size:
5318 1493 1416 1137 341 246 154 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7997 276
Size: 53 83 186 936 6463 2468
Fragments arranged by size:
6463 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BsiWI BstXI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI