(Linear) (Six-base) MAPSORT of: KIBB6015.seq Check: 5322 from: 1 to: 5484 KIBB6015.orf With 182 enzymes: SgfI * November 3, 2018 13:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1940 3498 3534 3735 4707 4968 5484 Size: 1940 1558 36 201 972 261 516 Fragments arranged by size: 1940 1558 972 516 261 201 36 AccI GT'mk_AC Cuts at: 0 5275 5484 Size: 5275 209 Acc65I G'GTAC_C Cuts at: 0 3441 5484 Size: 3441 2043 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 449 1790 2515 2659 2670 3100 3121 3673 Size: 449 1341 725 144 11 430 21 552 Cuts at: 3673 4060 4423 4765 5484 Size: 387 363 342 719 Fragments arranged by size: 1341 725 719 552 449 430 387 363 342 144 21 11 AfeI AGC'GCT Cuts at: 0 2749 5484 Size: 2749 2735 AflII C'TTAA_G Cuts at: 0 3466 3804 5484 Size: 3466 338 1680 Fragments arranged by size: 3466 1680 338 AflIII A'CryG_T Cuts at: 0 3452 5045 5484 Size: 3452 1593 439 Fragments arranged by size: 3452 1593 439 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1056 1238 5484 Size: 1056 182 4246 Fragments arranged by size: 4246 1056 182 AlwNI CAG_nnn'CTG Cuts at: 0 427 2040 4038 4068 4071 4083 4722 5484 Size: 427 1613 1998 30 3 12 639 762 Fragments arranged by size: 1998 1613 762 639 427 30 12 3 ApaI G_GGCC'C Cuts at: 0 1572 5484 Size: 1572 3912 ApaLI G'TGCA_C Cuts at: 0 1604 3722 5484 Size: 1604 2118 1762 Fragments arranged by size: 2118 1762 1604 ApoI r'AATT_y Cuts at: 0 817 971 1442 2113 5268 5484 Size: 817 154 471 671 3155 216 Fragments arranged by size: 3155 817 671 471 216 154 AseI AT'TA_AT Cuts at: 0 371 1487 5484 Size: 371 1116 3997 Fragments arranged by size: 3997 1116 371 AvaI C'yCGr_G Cuts at: 0 124 1025 5484 Size: 124 901 4459 Fragments arranged by size: 4459 901 124 BamHI G'GATC_C Cuts at: 0 405 2315 5484 Size: 405 1910 3169 Fragments arranged by size: 3169 1910 405 BanI G'GyrC_C Cuts at: 0 706 3441 4888 5484 Size: 706 2735 1447 596 Fragments arranged by size: 2735 1447 706 596 BanII G_rGCy'C Cuts at: 0 17 1572 5484 Size: 17 1555 3912 Fragments arranged by size: 3912 1555 17 BbsI GAAGACnn'nnnn_ Cuts at: 0 1398 2329 2902 3407 5484 Size: 1398 931 573 505 2077 Fragments arranged by size: 2077 1398 931 573 505 BbvCI CC'TCA_GC Cuts at: 0 229 801 5484 Size: 229 572 4683 Fragments arranged by size: 4683 572 229 BciVI GTATCCnnnnn_n' Cuts at: 0 35 5484 Size: 35 5449 BclI T'GATC_A Cuts at: 0 4494 4970 5484 Size: 4494 476 514 Fragments arranged by size: 4494 514 476 BfrBI ATG'CAT Cuts at: 0 376 1401 2658 3023 5484 Size: 376 1025 1257 365 2461 Fragments arranged by size: 2461 1257 1025 376 365 BglI GCCn_nnn'nGGC Cuts at: 0 565 5484 Size: 565 4919 BglII A'GATC_T Cuts at: 0 2240 4742 5484 Size: 2240 2502 742 Fragments arranged by size: 2502 2240 742 Bme1580I G_kGCm'C Cuts at: 0 1572 1608 3702 3726 4891 5484 Size: 1572 36 2094 24 1165 593 Fragments arranged by size: 2094 1572 1165 593 36 24 BmrI ACTGGGnnnn_n' Cuts at: 0 2954 5484 Size: 2954 2530 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2754 5484 Size: 2754 2730 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3016 3457 3514 4854 5076 5264 5484 Size: 3016 441 57 1340 222 188 220 Fragments arranged by size: 3016 1340 441 222 220 188 57 Bpu10I CC'TnA_GC Cuts at: 0 229 801 3146 3317 5484 Size: 229 572 2345 171 2167 Fragments arranged by size: 2345 2167 572 229 171 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3268 3601 5484 Size: 3268 333 1883 Fragments arranged by size: 3268 1883 333 BsaI GGTCTCn'nnnn_ Cuts at: 0 3815 5484 Size: 3815 1669 BsaAI yAC'GTr Cuts at: 0 302 2397 4411 5484 Size: 302 2095 2014 1073 Fragments arranged by size: 2095 2014 1073 302 BsaBI GATnn'nnATC Cuts at: 0 404 1020 5484 Size: 404 616 4464 Fragments arranged by size: 4464 616 404 BsaWI w'CCGG_w Cuts at: 0 2312 2984 5484 Size: 2312 672 2500 Fragments arranged by size: 2500 2312 672 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 92 122 1035 1065 1827 1857 3160 3190 Size: 92 30 913 30 762 30 1303 30 Cuts at: 3190 5035 5065 5484 Size: 1845 30 419 Fragments arranged by size: 1845 1303 913 762 419 92 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 778 1120 1806 2664 3553 3784 4005 4060 Size: 778 342 686 858 889 231 221 55 Cuts at: 4060 5484 Size: 1424 Fragments arranged by size: 1424 889 858 778 686 342 231 221 55 BseYI C'CCAG_C Cuts at: 0 3971 5363 5484 Size: 3971 1392 121 Fragments arranged by size: 3971 1392 121 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 918 1026 1912 2053 2746 3157 3614 5484 Size: 918 108 886 141 693 411 457 1870 Fragments arranged by size: 1870 918 886 693 457 411 141 108 BsiEI CG_ry'CG Cuts at: 0 2084 5484 Size: 2084 3400 BsiHKAI G_wGCw'C Cuts at: 0 17 257 1608 1892 2591 3551 3726 5053 Size: 17 240 1351 284 699 960 175 1327 Cuts at: 5053 5484 Size: 431 Fragments arranged by size: 1351 1327 960 699 431 284 240 175 17 BsmI GAATG_Cn' Cuts at: 0 1197 1511 1540 5484 Size: 1197 314 29 3944 Fragments arranged by size: 3944 1197 314 29 BsmBI CGTCTCn'nnnn_ Cuts at: 0 4584 5484 Size: 4584 900 Bsp1286I G_dGCh'C Cuts at: 0 17 257 1572 1608 1892 2591 3551 3702 Size: 17 240 1315 36 284 699 960 151 Cuts at: 3702 3726 4891 5053 5484 Size: 24 1165 162 431 Fragments arranged by size: 1315 1165 960 699 431 284 240 162 151 36 24 17 BspEI T'CCGG_A Cuts at: 0 2312 2984 5484 Size: 2312 672 2500 Fragments arranged by size: 2500 2312 672 BspHI T'CATG_A Cuts at: 0 240 5484 Size: 240 5244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1940 3498 3534 3735 4091 4707 4968 5484 Size: 1940 1558 36 201 356 616 261 516 Fragments arranged by size: 1940 1558 616 516 356 261 201 36 BsrDI GCAATG_nn' Cuts at: 0 1014 5238 5484 Size: 1014 4224 246 Fragments arranged by size: 4224 1014 246 BsrFI r'CCGG_y Cuts at: 0 2083 2254 4443 5484 Size: 2083 171 2189 1041 Fragments arranged by size: 2189 2083 1041 171 BsrGI T'GTAC_A Cuts at: 0 1580 3894 5484 Size: 1580 2314 1590 Fragments arranged by size: 2314 1590 1580 BssSI C'ACGA_G Cuts at: 0 4126 4756 5484 Size: 4126 630 728 Fragments arranged by size: 4126 728 630 BstAPI GCAn_nnn'nTGC Cuts at: 0 3513 4068 4758 5484 Size: 3513 555 690 726 Fragments arranged by size: 3513 726 690 555 BstEII G'GTnAC_C Cuts at: 0 3708 5484 Size: 3708 1776 BstYI r'GATC_y Cuts at: 0 156 405 2240 2315 4028 4742 5484 Size: 156 249 1835 75 1713 714 742 Fragments arranged by size: 1835 1713 742 714 249 156 75 BstZ17I GTA'TAC Cuts at: 0 5276 5484 Size: 5276 208 Bsu36I CC'TnA_GG Cuts at: 0 1818 5484 Size: 1818 3666 BtgI C'CryG_G Cuts at: 0 1447 4020 5484 Size: 1447 2573 1464 Fragments arranged by size: 2573 1464 1447 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1214 5484 Size: 1214 4270 BtsI GCAGTG_nn' Cuts at: 0 2036 4614 4680 5484 Size: 2036 2578 66 804 Fragments arranged by size: 2578 2036 804 66 ClaI AT'CG_AT Cuts at: 0 3042 5484 Size: 3042 2442 DraI TTT'AAA Cuts at: 0 822 5484 Size: 822 4662 DraIII CAC_nnn'GTG Cuts at: 0 1971 4414 5484 Size: 1971 2443 1070 Fragments arranged by size: 2443 1971 1070 EaeI y'GGCC_r Cuts at: 0 449 1208 1880 2081 2555 2699 4733 5484 Size: 449 759 672 201 474 144 2034 751 Fragments arranged by size: 2034 759 751 672 474 449 201 144 EagI C'GGCC_G Cuts at: 0 2081 5484 Size: 2081 3403 EarI CTCTTCn'nnn_ Cuts at: 0 755 2017 2918 3284 3982 4634 5484 Size: 755 1262 901 366 698 652 850 Fragments arranged by size: 1262 901 850 755 698 652 366 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2706 5484 Size: 2706 2778 EcoICRI GAG'CTC Cuts at: 0 15 5484 Size: 15 5469 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 449 1790 2515 2659 2670 3016 3100 3121 Size: 449 1341 725 144 11 346 84 21 Cuts at: 3121 3457 3514 3673 4060 4423 4765 4854 5076 Size: 336 57 159 387 363 342 89 222 Cuts at: 5076 5264 5484 Size: 188 220 Fragments arranged by size: 1341 725 449 387 363 346 342 336 222 220 188 159 144 89 84 57 21 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 4042 5038 5484 Size: 4042 996 446 Fragments arranged by size: 4042 996 446 EcoO109I rG'GnC_Cy Cuts at: 0 2952 3681 4014 5484 Size: 2952 729 333 1470 Fragments arranged by size: 2952 1470 729 333 EcoRV GAT'ATC Cuts at: 0 694 3232 5484 Size: 694 2538 2252 Fragments arranged by size: 2538 2252 694 FspI TGC'GCA Cuts at: 0 2437 3676 5484 Size: 2437 1239 1808 Fragments arranged by size: 2437 1808 1239 HaeII r_GCGC'y Cuts at: 0 2751 5484 Size: 2751 2733 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 33 65 92 124 683 708 715 740 Size: 33 32 27 32 559 25 7 25 Cuts at: 740 759 791 3394 3426 4255 4287 5035 5067 Size: 19 32 2603 32 829 32 748 32 Cuts at: 5067 5484 Size: 417 Fragments arranged by size: 2603 829 748 559 417 33 32 32 32 32 32 32 27 25 25 19 7 HincII GTy'rAC Cuts at: 0 4311 5092 5484 Size: 4311 781 392 Fragments arranged by size: 4311 781 392 HindIII A'AGCT_T Cuts at: 0 434 1637 5484 Size: 434 1203 3847 Fragments arranged by size: 3847 1203 434 KpnI G_GTAC'C Cuts at: 0 3445 5484 Size: 3445 2039 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 620 993 1674 1825 3864 4326 5484 Size: 620 373 681 151 2039 462 1158 Fragments arranged by size: 2039 1158 681 620 462 373 151 MscI TGG'CCA Cuts at: 0 451 1210 1882 2557 2701 4735 5484 Size: 451 759 672 675 144 2034 749 Fragments arranged by size: 2034 759 749 675 672 451 144 MslI CAynn'nnrTG Cuts at: 0 1093 1220 2383 2482 2770 4001 5484 Size: 1093 127 1163 99 288 1231 1483 Fragments arranged by size: 1483 1231 1163 1093 288 127 99 MspA1I CmG'CkG Cuts at: 0 345 1040 2040 2793 3066 3753 3975 4068 Size: 345 695 1000 753 273 687 222 93 Cuts at: 4068 4089 5367 5484 Size: 21 1278 117 Fragments arranged by size: 1278 1000 753 695 687 345 273 222 117 93 21 NcoI C'CATG_G Cuts at: 0 1447 5484 Size: 1447 4037 NsiI A_TGCA'T Cuts at: 0 378 1403 2660 3025 5484 Size: 378 1025 1257 365 2459 Fragments arranged by size: 2459 1257 1025 378 365 NspI r_CATG'y Cuts at: 0 1405 2051 2180 2367 2481 2658 2662 3037 Size: 1405 646 129 187 114 177 4 375 Cuts at: 3037 3313 3456 4477 4604 4806 5049 5070 5484 Size: 276 143 1021 127 202 243 21 414 Fragments arranged by size: 1405 1021 646 414 375 276 243 202 187 177 143 129 127 114 21 4 PciI A'CATG_T Cuts at: 0 3452 5045 5484 Size: 3452 1593 439 Fragments arranged by size: 3452 1593 439 PflMI CCAn_nnn'nTGG Cuts at: 0 38 5484 Size: 38 5446 PfoI T'CCnGG_A Cuts at: 0 2057 3962 4434 5053 5484 Size: 2057 1905 472 619 431 Fragments arranged by size: 2057 1905 619 472 431 PmlI CAC'GTG Cuts at: 0 4411 5484 Size: 4411 1073 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 4670 4702 5484 Size: 4670 32 782 Fragments arranged by size: 4670 782 32 PpuMI rG'GwC_Cy Cuts at: 0 2952 5484 Size: 2952 2532 PsiI TTA'TAA Cuts at: 0 92 5484 Size: 92 5392 PspOMI G'GGCC_C Cuts at: 0 1568 5484 Size: 1568 3916 PspXI vC'TCGA_Gb Cuts at: 0 124 5484 Size: 124 5360 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2809 2841 5484 Size: 2809 32 2643 Fragments arranged by size: 2809 2643 32 PstI C_TGCA'G Cuts at: 0 1184 2798 2966 3980 4088 4124 5484 Size: 1184 1614 168 1014 108 36 1360 Fragments arranged by size: 1614 1360 1184 1014 168 108 36 PvuII CAG'CTG Cuts at: 0 345 1040 2040 2793 3066 3753 3975 4068 Size: 345 695 1000 753 273 687 222 93 Cuts at: 4068 4089 5367 5484 Size: 21 1278 117 Fragments arranged by size: 1278 1000 753 695 687 345 273 222 117 93 21 RsrII CG'GwC_CG Cuts at: 0 4194 5484 Size: 4194 1290 SacI G_AGCT'C Cuts at: 0 17 5484 Size: 17 5467 ScaI AGT'ACT Cuts at: 0 2470 5484 Size: 2470 3014 SfcI C'TryA_G Cuts at: 0 1180 1381 2794 2962 3976 4084 4120 5484 Size: 1180 201 1413 168 1014 108 36 1364 Fragments arranged by size: 1413 1364 1180 1014 201 168 108 36 SmlI C'TyrA_G Cuts at: 0 124 3283 3466 3616 3804 5484 Size: 124 3159 183 150 188 1680 Fragments arranged by size: 3159 1680 188 183 150 124 SnaBI TAC'GTA Cuts at: 0 302 5484 Size: 302 5182 SphI G_CATG'C Cuts at: 0 2367 2658 2662 4806 5070 5484 Size: 2367 291 4 2144 264 414 Fragments arranged by size: 2367 2144 414 291 264 4 StuI AGG'CCT Cuts at: 0 931 5484 Size: 931 4553 StyI C'CwwG_G Cuts at: 0 167 1447 2444 3563 3845 4338 5484 Size: 167 1280 997 1119 282 493 1146 Fragments arranged by size: 1280 1146 1119 997 493 282 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 385 1081 3361 4926 5484 Size: 385 696 2280 1565 558 Fragments arranged by size: 2280 1565 696 558 385 TatI w'GTAC_w Cuts at: 0 1580 2468 3894 4628 5484 Size: 1580 888 1426 734 856 Fragments arranged by size: 1580 1426 888 856 734 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1035 1067 1663 1695 1827 1859 5484 Size: 1035 32 596 32 132 32 3625 Fragments arranged by size: 3625 1035 596 132 32 32 32 XbaI T'CTAG_A Cuts at: 0 153 1515 2271 5484 Size: 153 1362 756 3213 Fragments arranged by size: 3213 1362 756 153 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 59 603 3882 4152 4698 5460 5484 Size: 59 544 3279 270 546 762 24 Fragments arranged by size: 3279 762 546 544 270 59 24 XhoI C'TCGA_G Cuts at: 0 124 5484 Size: 124 5360 XmnI GAAnn'nnTTC Cuts at: 0 1442 1688 1772 2909 3250 3404 4820 5484 Size: 1442 246 84 1137 341 154 1416 664 Fragments arranged by size: 1442 1416 1137 664 341 246 154 84 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflII AflIII AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI BfrBI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I NcoI NsiI NspI PciI PflMI PfoI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuII RsrII SacI ScaI SfcI SmlI SnaBI SphI StuI StyI TaqII TatI TstI XbaI XcmI XhoI XmnI Enzymes that do not cut: AatII AclI AgeI AhdI AleI AloI AscI AsiSI AvrII BaeI BbeI BcgI BlpI BmgBI BmtI BsaHI BsiWI BsrBI BssHII BstBI BstXI CspCI DrdI EcoRI FalI FseI FspAI HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PmeI PshAI PvuI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SrfI SspI SwaI Tth111I XmaI ZraI