################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AcsI 445 AAATTT [GA]^AATT[CT] ApoI 445 AAATTT [GA]^AATT[CT] BamHI 141 GGATCC G^GATCC BanI 768 GGTGCC G^G[CT][GA]CC BanII 182 GAGCCC G[GA]GC[CT]^C BfaI 853 CTAG C^TAG BmyI 182 GAGCCC G[AGT]GC[ACT]^C BsmFI 32 GGGAC GGGAC(10/14) Bsp1286I 182 GAGCCC G[AGT]GC[ACT]^C BspHI 591 TCATGA T^CATGA BsrDI 757 GCAATG GCAATG(2/0) BstEII 267 GGTTACC G^GT[ACGT]ACC BstPI 267 GGTTACC G^GT[ACGT]ACC Cac8I 510 GCAGGC GC[ACGT]^[ACGT]GC CfoI 902 GCGC GCG^C DraI 256 TTTAAA TTT^AAA DraIII 930 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 454 CCACGG C^C[GA][CT]GG EarI 429 GAAGAG CTCTTC(1/4) EcoO65I 267 GGTTACC G^GT[ACGT]ACC EcoT14I 263 CCAAGG C^C[AT][AT]GG HhaI 902 GCGC GCG^C HinP1I 902 GCGC G^CGC HphI 839 TCACC GGTGA(8/7) Ksp632I 429 GAAGAG CTCTTC(1/4) MaeI 853 CTAG C^TAG PpuMI 32 GGGACCC [GA]G^G[AT]CC[CT] PvuII 629 CAGCTG CAG^CTG RcaI 591 TCATGA T^CATGA SexAI 931 ACCAGGT A^CC[AT]GGT SfaNI 229 GATGC GCATC(5/9) StyI 263 CCAAGG C^C[AT][AT]GG SwaI 255 ATTTAAAT ATTT^AAAT Tsp45I 482 GTGAC ^GT[GC]AC TspRI 365 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 749 CCATATCAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 326 ACGTGT A^C[GA][CT]GT 527 ACACGT AvaII 33 GGACC G^G[AT]CC 503 GGACC BcnI 30 CCGGG CC^[GC]GG 710 CCCGG BsmAI 21 GAGAC GTCTC(1/5) 394 GAGAC BsmBI 21 GAGACG CGTCTC(1/5) 394 GAGACG BsrI 84 CCAGT ACTGG(1/-1) 621 CCAGT DdeI 114 CTCAG C^T[ACGT]AG 521 CTCAG DraII 32 GGGACCC [GA]G^G[ACGT]CC[CT] 512 AGGCCCC EcoO109I 32 GGGACCC [GA]G^G[ACGT]CC[CT] 512 AGGCCCC HaeIII 499 GGCC GG^CC 513 GGCC MaeIII 268 GTTAC ^GT[ACGT]AC 482 GTGAC MboII 426 GAAGA GAAGA(8/7) 429 GAAGA MseI 257 TTAA T^TAA 443 TTAA MspA1I 70 CAGCGG C[AC]G^C[GT]G 629 CAGCTG NciI 30 CCGGG CC^[GC]GG 710 CCCGG PleI 595 GACTC GAGTC(4/5) 155 GAGTC TaqI 567 TCGA T^CGA 863 TCGA TfiI 460 GATTC G^A[AT]TC 836 GAATC Tru9I 257 TTAA T^TAA 443 TTAA TthHB8I 567 TCGA T^CGA 863 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI BstYI Cfr13I DpnII EcoRII FauI Fnu4HI FokI HapII HinfI HpaII ItaI MaeII MboI MflI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI Tsp509I XhoII