################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AocI Aor51HI AscI AseI AsnI Asp718I AspI AviII AvrII BbeI BbrPI BbvCI BcgI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BseAI BsiWI BsmBI BspEI Bst1107I BstEII BstPI BstZ17I Bsu36I CelII CpoI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI MluI MroI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII SacI SbfI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspI StuI SwaI Tsp45I Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AgeI 569 ACCGGT A^CCGGT AhdI 457 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1715 GTGCAC G^TGCAC ApaLI 1715 GTGCAC G^TGCAC AspEI 457 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 2370 CCCGAG C^[CT]CG[GA]G BalI 1926 TGGCCA TGG^CCA BclI 381 TGATCA T^GATCA BcnI 4058 CCCGG CC^[GC]GG BsaBI 1240 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 569 ACCGGT [AT]^CCGG[AT] BsiEI 1639 CGATCG CG[GA][CT]^CG BsoBI 2370 CCCGAG C^[CT]CG[GA]G BspDI 1073 ATCGAT AT^CGAT BsrBI 1656 GAGCGG CCGCTC(-3/-3) BsrFI 569 ACCGGT [GA]^CCGG[CT] BssHII 1231 GCGCGC G^CGCGC BssSI 34 CTCGTG CACGAG(-5/-1) BstAPI 1577 GCAGTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 3790 CCAGTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 569 ACCGGT [GA]^CCGG[CT] ClaI 1073 ATCGAT AT^CGAT EaeI 1926 TGGCCA [CT]^GGCC[GA] Eam1105I 457 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 231 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 381 TGATCA T^GATCA HaeII 532 AGCGCC [GA]GCGC^[CT] HgaI 404 GCGTC GACGC(5/10) KspI 140 CCGCGG CCGC^GG MaeIII 2210 GTTAC ^GT[ACGT]AC MamI 1240 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2072 CAATTG C^AATTG MluNI 1926 TGGCCA TGG^CCA MscI 1926 TGGCCA TGG^CCA MunI 2072 CAATTG C^AATTG NciI 4058 CCCGG CC^[GC]GG NdeI 1746 CATATG CA^TATG PflMI 1540 CCATTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 569 ACCGGT A^CCGGT PpuMI 415 GGGACCT [GA]G^G[AT]CC[CT] PvuI 1639 CGATCG CGAT^CG PvuII 2476 CAGCTG CAG^CTG SacII 140 CCGCGG CCGC^GG SalI 364 GTCGAC G^TCGAC SapI 499 GAAGAGC GCTCTTC(1/4) ScaI 2263 AGTACT AGT^ACT SpeI 3160 ACTAGT A^CTAGT Van91I 1540 CCATTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 4201 CCAGCCTCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 364 GTCGAC GT^[AC][GT]AC 3055 GTAGAC AlwNI 837 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1052 CAGATTCTG ApaI 977 GGGCCC GGGCC^C 3268 GGGCCC Asp700I 226 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2953 GAATTAGTTC BamHI 1965 GGATCC G^GATCC 2528 GGATCC BciVI 3129 GTATCC GTATCC(6/5) 4146 GTATCC BsaI 1343 GAGACC GGTCTC(1/5) 3506 GGTCTC Bsp1407I 2559 TGTACA T^GTACA 3762 TGTACA BspHI 1179 TCATGA T^CATGA 3443 TCATGA BsrGI 2559 TGTACA T^GTACA 3762 TGTACA BstBI 3548 TTCGAA TT^CGAA 3560 TTCGAA DrdI 687 GACGGACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3138 GACAGACATGTC DsaI 140 CCGCGG C^C[GA][CT]GG 1929 CCACGG EarI 499 GAAGAG CTCTTC(1/4) 3308 GAAGAG EcoRI 2055 GAATTC G^AATTC 2355 GAATTC EcoRV 639 GATATC GAT^ATC 4062 GATATC FauI 10 CCCGC CCCGC(4/6) 1658 GCGGG HapII 570 CCGG C^CGG 4059 CCGG HpaII 570 CCGG C^CGG 4059 CCGG Ksp632I 499 GAAGAG CTCTTC(1/4) 3308 GAAGAG MaeII 3911 ACGT A^CGT 4127 ACGT MspI 570 CCGG C^CGG 4059 CCGG NspV 3548 TTCGAA TT^CGAA 3560 TTCGAA RcaI 1179 TCATGA T^CATGA 3443 TCATGA SfuI 3548 TTCGAA TT^CGAA 3560 TTCGAA SspBI 2559 TGTACA T^GTACA 3762 TGTACA XbaI 804 TCTAGA T^CTAGA 2059 TCTAGA XmnI 226 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2953 GAATTAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaII BanI BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI MaeI MboI MboII MflI MnlI MseI MslI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII