(Linear) (Six-base) MAPSORT of: KIBB5617.seq Check: 6523 from: 1 to: 4224 KIBB5617.orf With 182 enzymes: SgfI * April 1, 2016 14:38 .. AccI GT'mk_AC Cuts at: 0 365 3056 4224 Size: 365 2691 1168 Fragments arranged by size: 2691 1168 365 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 934 2190 2675 2741 4224 Size: 217 717 1256 485 66 1483 Fragments arranged by size: 1483 1256 717 485 217 66 AflIII A'CryG_T Cuts at: 0 429 2086 3143 3737 4224 Size: 429 1657 1057 594 487 Fragments arranged by size: 1657 1057 594 487 429 AgeI A'CCGG_T Cuts at: 0 569 4224 Size: 569 3655 AhdI GACnn_n'nnGTC Cuts at: 0 462 4224 Size: 462 3762 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 52 4224 Size: 52 4172 AlwNI CAG_nnn'CTG Cuts at: 0 842 1057 4224 Size: 842 215 3167 Fragments arranged by size: 3167 842 215 ApaI G_GGCC'C Cuts at: 0 981 3272 4224 Size: 981 2291 952 Fragments arranged by size: 2291 981 952 ApaLI G'TGCA_C Cuts at: 0 1715 4224 Size: 1715 2509 ApoI r'AATT_y Cuts at: 0 603 1212 1431 1783 2055 2237 2355 2841 Size: 603 609 219 352 272 182 118 486 Cuts at: 2841 3365 3552 3697 4224 Size: 524 187 145 527 Fragments arranged by size: 609 603 527 524 486 352 272 219 187 182 145 118 AvaI C'yCGr_G Cuts at: 0 2370 4224 Size: 2370 1854 BamHI G'GATC_C Cuts at: 0 1965 2528 4224 Size: 1965 563 1696 Fragments arranged by size: 1965 1696 563 BanI G'GyrC_C Cuts at: 0 193 1619 4013 4224 Size: 193 1426 2394 211 Fragments arranged by size: 2394 1426 211 193 BanII G_rGCy'C Cuts at: 0 506 981 3272 3897 4224 Size: 506 475 2291 625 327 Fragments arranged by size: 2291 625 506 475 327 BbsI GAAGACnn'nnnn_ Cuts at: 0 233 3601 3960 4224 Size: 233 3368 359 264 Fragments arranged by size: 3368 359 264 233 BciVI GTATCCnnnnn_n' Cuts at: 0 3140 4157 4224 Size: 3140 1017 67 Fragments arranged by size: 3140 1017 67 BclI T'GATC_A Cuts at: 0 381 4224 Size: 381 3843 BglII A'GATC_T Cuts at: 0 1676 2940 3543 3591 4224 Size: 1676 1264 603 48 633 Fragments arranged by size: 1676 1264 633 603 48 Bme1580I G_kGCm'C Cuts at: 0 981 1719 3012 3272 4224 Size: 981 738 1293 260 952 Fragments arranged by size: 1293 981 952 738 260 BmrI ACTGGGnnnn_n' Cuts at: 0 266 1526 3266 4224 Size: 266 1260 1740 958 Fragments arranged by size: 1740 1260 958 266 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 907 1141 1445 4224 Size: 907 234 304 2779 Fragments arranged by size: 2779 907 304 234 Bpu10I CC'TnA_GC Cuts at: 0 1654 4224 Size: 1654 2570 BsaI GGTCTCn'nnnn_ Cuts at: 0 1337 3512 4224 Size: 1337 2175 712 Fragments arranged by size: 2175 1337 712 BsaBI GATnn'nnATC Cuts at: 0 1244 4224 Size: 1244 2980 BsaWI w'CCGG_w Cuts at: 0 569 4224 Size: 569 3655 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 533 563 4224 Size: 533 30 3661 Fragments arranged by size: 3661 533 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 75 2975 3859 3905 4224 Size: 75 2900 884 46 319 Fragments arranged by size: 2900 884 319 75 46 BseYI C'CCAG_C Cuts at: 0 505 980 1754 2133 4224 Size: 505 475 774 379 2091 Fragments arranged by size: 2091 774 505 475 379 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 1812 3145 3271 4224 Size: 76 1736 1333 126 953 Fragments arranged by size: 1736 1333 953 126 76 BsiEI CG_ry'CG Cuts at: 0 1642 4224 Size: 1642 2582 BsiHKAI G_wGCw'C Cuts at: 0 41 1187 1719 1852 2329 3225 3869 4097 Size: 41 1146 532 133 477 896 644 228 Cuts at: 4097 4224 Size: 127 Fragments arranged by size: 1146 896 644 532 477 228 133 127 41 BsmI GAATG_Cn' Cuts at: 0 633 864 1090 1654 4224 Size: 633 231 226 564 2570 Fragments arranged by size: 2570 633 564 231 226 Bsp1286I G_dGCh'C Cuts at: 0 41 506 981 1187 1719 1852 2329 3012 Size: 41 465 475 206 532 133 477 683 Cuts at: 3012 3225 3272 3869 3897 4097 4224 Size: 213 47 597 28 200 127 Fragments arranged by size: 683 597 532 477 475 465 213 206 200 133 127 47 41 28 BspHI T'CATG_A Cuts at: 0 1179 3443 4224 Size: 1179 2264 781 Fragments arranged by size: 2264 1179 781 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 427 1380 2759 4224 Size: 427 953 1379 1465 Fragments arranged by size: 1465 1379 953 427 BsrBI CCG'CTC Cuts at: 0 1658 4224 Size: 1658 2566 BsrDI GCAATG_nn' Cuts at: 0 72 628 2619 4224 Size: 72 556 1991 1605 Fragments arranged by size: 1991 1605 556 72 BsrFI r'CCGG_y Cuts at: 0 569 4224 Size: 569 3655 BsrGI T'GTAC_A Cuts at: 0 2559 3762 4224 Size: 2559 1203 462 Fragments arranged by size: 2559 1203 462 BssHII G'CGCG_C Cuts at: 0 1231 4224 Size: 1231 2993 BssSI C'ACGA_G Cuts at: 0 34 4224 Size: 34 4190 BstAPI GCAn_nnn'nTGC Cuts at: 0 1583 4224 Size: 1583 2641 BstBI TT'CG_AA Cuts at: 0 3549 3561 4224 Size: 3549 12 663 Fragments arranged by size: 3549 663 12 BstXI CCAn_nnnn'nTGG Cuts at: 0 3797 4224 Size: 3797 427 BstYI r'GATC_y Cuts at: 0 919 1535 1676 1965 2220 2528 2940 3543 Size: 919 616 141 289 255 308 412 603 Cuts at: 3543 3591 4224 Size: 48 633 Fragments arranged by size: 919 633 616 603 412 308 289 255 141 48 BtgI C'CryG_G Cuts at: 0 140 1929 4224 Size: 140 1789 2295 Fragments arranged by size: 2295 1789 140 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2413 4224 Size: 2413 1811 BtsI GCAGTG_nn' Cuts at: 0 474 1185 2955 3254 4224 Size: 474 711 1770 299 970 Fragments arranged by size: 1770 970 711 474 299 ClaI AT'CG_AT Cuts at: 0 1074 4224 Size: 1074 3150 DraI TTT'AAA Cuts at: 0 218 1793 2096 4224 Size: 218 1575 303 2128 Fragments arranged by size: 2128 1575 303 218 DrdI GACnn_nn'nnGTC Cuts at: 0 693 3144 4224 Size: 693 2451 1080 Fragments arranged by size: 2451 1080 693 EaeI y'GGCC_r Cuts at: 0 1926 4224 Size: 1926 2298 EarI CTCTTCn'nnn_ Cuts at: 0 494 3303 4224 Size: 494 2809 921 Fragments arranged by size: 2809 921 494 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 907 934 1141 1445 2190 2675 2741 Size: 217 690 27 207 304 745 485 66 Cuts at: 2741 4224 Size: 1483 Fragments arranged by size: 1483 745 690 485 304 217 207 66 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 235 4224 Size: 235 3989 EcoO109I rG'GnC_Cy Cuts at: 0 6 416 977 4224 Size: 6 410 561 3247 Fragments arranged by size: 3247 561 410 6 EcoRI G'AATT_C Cuts at: 0 2055 2355 4224 Size: 2055 300 1869 Fragments arranged by size: 2055 1869 300 EcoRV GAT'ATC Cuts at: 0 641 4064 4224 Size: 641 3423 160 Fragments arranged by size: 3423 641 160 HaeII r_GCGC'y Cuts at: 0 536 4224 Size: 536 3688 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 448 480 533 565 1653 1685 3561 3593 Size: 448 32 53 32 1088 32 1876 32 Cuts at: 3593 3838 3870 4224 Size: 245 32 354 Fragments arranged by size: 1876 1088 448 354 245 53 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 366 408 1110 1134 1281 2425 4224 Size: 366 42 702 24 147 1144 1799 Fragments arranged by size: 1799 1144 702 366 147 42 24 MfeI C'AATT_G Cuts at: 0 2072 4224 Size: 2072 2152 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 446 2269 2664 3326 3604 4224 Size: 446 1823 395 662 278 620 Fragments arranged by size: 1823 662 620 446 395 278 MscI TGG'CCA Cuts at: 0 1928 4224 Size: 1928 2296 MslI CAynn'nnrTG Cuts at: 0 34 349 877 1866 2386 3293 3464 4224 Size: 34 315 528 989 520 907 171 760 Fragments arranged by size: 989 907 760 528 520 315 171 34 MspA1I CmG'CkG Cuts at: 0 13 142 2478 3513 4224 Size: 13 129 2336 1035 711 Fragments arranged by size: 2336 1035 711 129 13 NdeI CA'TA_TG Cuts at: 0 1747 4224 Size: 1747 2477 NspI r_CATG'y Cuts at: 0 433 2090 2423 3147 3259 3741 4224 Size: 433 1657 333 724 112 482 483 Fragments arranged by size: 1657 724 483 482 433 333 112 PasI CC'CwG_GG Cuts at: 0 537 4224 Size: 537 3687 PciI A'CATG_T Cuts at: 0 429 2086 3143 3737 4224 Size: 429 1657 1057 594 487 Fragments arranged by size: 1657 1057 594 487 429 PflMI CCAn_nnn'nTGG Cuts at: 0 1546 4224 Size: 1546 2678 PfoI T'CCnGG_A Cuts at: 0 1439 4057 4224 Size: 1439 2618 167 Fragments arranged by size: 2618 1439 167 PpuMI rG'GwC_Cy Cuts at: 0 416 4224 Size: 416 3808 PspOMI G'GGCC_C Cuts at: 0 977 3268 4224 Size: 977 2291 956 Fragments arranged by size: 2291 977 956 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1242 1274 4224 Size: 1242 32 2950 Fragments arranged by size: 2950 1242 32 PstI C_TGCA'G Cuts at: 0 304 1561 1579 4224 Size: 304 1257 18 2645 Fragments arranged by size: 2645 1257 304 18 PvuI CG_AT'CG Cuts at: 0 1642 4224 Size: 1642 2582 PvuII CAG'CTG Cuts at: 0 2478 4224 Size: 2478 1746 SacII CC_GC'GG Cuts at: 0 143 4224 Size: 143 4081 SalI G'TCGA_C Cuts at: 0 364 4224 Size: 364 3860 SapI GCTCTTCn'nnn_ Cuts at: 0 494 4224 Size: 494 3730 ScaI AGT'ACT Cuts at: 0 2265 4224 Size: 2265 1959 SfcI C'TryA_G Cuts at: 0 300 1557 1575 3029 3053 3756 4224 Size: 300 1257 18 1454 24 703 468 Fragments arranged by size: 1454 1257 703 468 300 24 18 SpeI A'CTAG_T Cuts at: 0 3160 4224 Size: 3160 1064 StyI C'CwwG_G Cuts at: 0 50 189 1812 2811 4224 Size: 50 139 1623 999 1413 Fragments arranged by size: 1623 1413 999 139 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 165 290 3445 4224 Size: 165 125 3155 779 Fragments arranged by size: 3155 779 165 125 TatI w'GTAC_w Cuts at: 0 248 1948 2089 2263 2559 2622 2976 3762 Size: 248 1700 141 174 296 63 354 786 Cuts at: 3762 4224 Size: 462 Fragments arranged by size: 1700 786 462 354 296 248 174 141 63 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 14 46 1863 1895 4224 Size: 14 32 1817 32 2329 Fragments arranged by size: 2329 1817 32 32 14 XbaI T'CTAG_A Cuts at: 0 804 2059 4224 Size: 804 1255 2165 Fragments arranged by size: 2165 1255 804 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 4208 4224 Size: 4208 16 XmnI GAAnn'nnTTC Cuts at: 0 230 2957 4224 Size: 230 2727 1267 Fragments arranged by size: 2727 1267 230 Enzymes that do cut: AccI AcuI AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BciVI BclI BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII MfeI MmeI MscI MslI MspA1I NdeI NspI PasI PciI PflMI PfoI PpuMI PspOMI PsrI PstI PvuI PvuII SacII SalI SapI ScaI SfcI SpeI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AleI AloI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BfrBI BglI BlpI BmgBI BmtI BplI BpuEI BsaAI BsaHI BsiWI BsmBI BspEI BstEII BstZ17I Bsu36I CspCI DraIII EagI EciI EcoICRI FalI FseI FspI FspAI HindIII HpaI KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI RsrII SacI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI SwaI Tth111I XhoI XmaI ZraI