Restriction map for
pFN21AE2637
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# NO CUT ENZYMES ( 87 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AgeI Alw44I Aor51HI ApaLI ApoI
AscI Asp700I Asp718I AviII AvrII
BamHI BbeI BbvCI BciVI BclI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsmI BspDI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstAPI
BstZ17I ClaI CpoI DraI DraIII
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NruI NsiI PacI PaeR7I
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfoI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 667 GACGTC GACGT^C
AcyI 667 GACGTC G[GA]^CG[CT]C
AhdI 2239 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 518 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1783 ATTAAT AT^TAAT
AsnI 1783 ATTAAT AT^TAAT
AspEI 2239 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1993 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 787 GGTGCC G^G[CT][GA]CC
BcgI 2241 CGAGGTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 4 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC
BsaHI 667 GACGTC G[GA]^CG[CT]C
BsaI 581 GAGACC GGTCTC(1/5)
BsiEI 540 CGGCCG CG[GA][CT]^CG
BsiWI 50 CGTACG C^GTACG
Bsp1407I 2129 TGTACA T^GTACA
BspMI 584 ACCTGC ACCTGC(4/8)
BsrDI 2177 GCAATG GCAATG(2/0)
BsrFI 2191 ACCGGC [GA]^CCGG[CT]
BsrGI 2129 TGTACA T^GTACA
BstBI 22 TTCGAA TT^CGAA
BstEII 1902 GGTGACC G^GT[ACGT]ACC
BstPI 1902 GGTGACC G^GT[ACGT]ACC
CelII 4 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2191 ACCGGC [GA]^CCGG[CT]
DrdI 661 GACCGCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 540 CGGCCG C^GGCCG
Eam1105I 2239 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1326 CTCTTC CTCTTC(1/4)
EclXI 540 CGGCCG C^GGCCG
Eco52I 540 CGGCCG C^GGCCG
EcoO65I 1902 GGTGACC G^GT[ACGT]ACC
EcoT14I 704 CCAAGG C^C[AT][AT]GG
HgaI 118 GACGC GACGC(5/10)
Hin1I 667 GACGTC G[GA]^CG[CT]C
HincII 1888 GTTGAC GT[CT]^[GA]AC
HindII 1888 GTTGAC GT[CT]^[GA]AC
HindIII 1978 AAGCTT A^AGCTT
Ksp632I 1326 CTCTTC CTCTTC(1/4)
MseI 1784 TTAA T^TAA
MslI 2199 CATCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 2232 GCTAGC G^CTAGC
NotI 539 GCGGCCGC GC^GGCCGC
NspI 455 GCATGC [GA]CATG^[CT]
NspV 22 TTCGAA TT^CGAA
PflFI 1993 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1945 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1783 ATTAAT AT^TAAT
PvuII 305 CAGCTG CAG^CTG
SacI 248 GAGCTC GAGCT^C
SfiI 1432 GGCCAGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 22 TTCGAA TT^CGAA
SphI 455 GCATGC GCATG^C
SspBI 2129 TGTACA T^GTACA
StyI 704 CCAAGG C^C[AT][AT]GG
Tru9I 1784 TTAA T^TAA
Tsp509I 1782 AATT ^AATT
Tth111I 1993 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1945 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccI 1876 GTCTAC GT^[AC][GT]AC
2209 GTAGAC
AocI 478 CCTGAGG CC^T[ACGT]AGG
523 CCTGAGG
AspHI 201 GAGCAC G[AT]GC[AT]^C
248 GAGCTC
BalI 1953 TGGCCA TGG^CCA
2258 TGGCCA
BbrPI 361 CACGTG CAC^GTG
910 CACGTG
BfaI 577 CTAG C^TAG
2233 CTAG
BsaAI 361 CACGTG [CT]AC^GT[GA]
910 CACGTG
BsiHKAI 201 GAGCAC G[AT]GC[AT]^C
248 GAGCTC
BsmBI 1323 CGTCTC CGTCTC(1/5)
2159 CGTCTC
BsrBI 1481 CCGCTC CCGCTC(-3/-3)
1575 CCGCTC
BstXI 1619 CCAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2034 CCACGTCCCTGG
Bsu36I 478 CCTGAGG CC^T[ACGT]AGG
523 CCTGAGG
CfoI 271 GCGC GCG^C
946 GCGC
Eco81I 478 CCTGAGG CC^T[ACGT]AGG
523 CCTGAGG
HhaI 271 GCGC GCG^C
946 GCGC
HinP1I 271 GCGC G^CGC
946 GCGC
KspI 1087 CCGCGG CCGC^GG
1411 CCGCGG
MaeI 577 CTAG C^TAG
2233 CTAG
MluNI 1953 TGGCCA TGG^CCA
2258 TGGCCA
MscI 1953 TGGCCA TGG^CCA
2258 TGGCCA
PmaCI 361 CACGTG CAC^GTG
910 CACGTG
PmlI 361 CACGTG CAC^GTG
910 CACGTG
PstI 142 CTGCAG CTGCA^G
1678 CTGCAG
SacII 1087 CCGCGG CCGC^GG
1411 CCGCGG
SmlI 562 CTCAAG C^T[CT][GA]AG
1375 CTCAAG
StuI 707 AGGCCT AGG^CCT
1219 AGGCCT
TfiI 133 GAATC G^A[AT]TC
1461 GATTC
XcmI 1812 CCAGTCCAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2001 CCAGTCGAAGATTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI ApaI AvaI AvaII BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII XmaCI XmaI