(Circular) (Six-base) MAPSORT of: pFN21AE2637.seq Check: 2285 from: 1 to: 6979 pFN21AE2637 6979 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2676 4789 278 Size: 53 83 186 936 1140 2113 2468 Fragments arranged by size: 2468 2113 1140 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3882 4215 4322 1937 Size: 1945 333 107 4594 Fragments arranged by size: 4594 1945 333 107 Acc65I G'GTAC_C Cuts at: 4300 4300 Size: 6979 AclI AA'CG_TT Cuts at: 1563 5106 5479 1563 Size: 3543 373 3063 Fragments arranged by size: 3543 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2334 2633 3734 3773 5042 6426 6694 1632 Size: 702 299 1101 39 1269 1384 268 1917 Fragments arranged by size: 1917 1384 1269 1101 702 299 268 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6782 AflIII A'CryG_T Cuts at: 1203 3194 3850 4161 5878 6855 1203 Size: 1991 656 311 1717 977 1327 Fragments arranged by size: 1991 1717 1327 977 656 311 AgeI A'CCGG_T Cuts at: 5810 5810 Size: 6979 AhdI GACnn_n'nnGTC Cuts at: 4249 5708 4249 Size: 1459 5520 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6979 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3099 1184 Size: 1915 5064 AlwNI CAG_nnn'CTG Cuts at: 2528 4722 6294 2528 Size: 2194 1572 3213 Fragments arranged by size: 3213 2194 1572 ApaI G_GGCC'C Cuts at: 2492 2865 2995 3023 2492 Size: 373 130 28 6448 Fragments arranged by size: 6448 373 130 28 ApaLI G'TGCA_C Cuts at: 5036 6192 5036 Size: 1156 5823 ApoI r'AATT_y Cuts at: 1493 4288 4514 4666 6775 1493 Size: 2795 226 152 2109 1697 Fragments arranged by size: 2795 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3789 5533 160 Size: 872 2757 1744 1606 Fragments arranged by size: 2757 1744 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6979 AvaI C'yCGr_G Cuts at: 1870 1943 3015 3615 3856 4304 1870 Size: 73 1072 600 241 448 4545 Fragments arranged by size: 4545 1072 600 448 241 73 BamHI G'GATC_C Cuts at: 4309 4676 4309 Size: 367 6612 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2792 4300 5755 618 Size: 325 217 627 1005 1508 1455 1842 Fragments arranged by size: 1842 1508 1455 1005 627 325 217 BanII G_rGCy'C Cuts at: 1391 2016 2186 2257 2473 2492 2856 2865 2995 Size: 625 170 71 216 19 364 9 130 Cuts at: 2995 3023 3066 3624 4298 1391 Size: 28 43 558 674 4072 Fragments arranged by size: 4072 674 625 558 364 216 170 130 71 43 28 19 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2355 2670 3657 928 Size: 971 456 315 987 4250 Fragments arranged by size: 4250 987 971 456 315 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4235 4269 5170 5204 1903 Size: 34 2298 34 901 34 3678 Fragments arranged by size: 3678 2298 901 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4871 6081 4871 Size: 1210 5769 BclI T'GATC_A Cuts at: 1548 1548 Size: 6979 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2860 3444 4262 Size: 107 122 71 631 605 1188 584 818 Cuts at: 4262 5590 136 Size: 1328 1525 Fragments arranged by size: 1525 1328 1188 818 631 605 584 122 107 71 BglII A'GATC_T Cuts at: 6974 6974 Size: 6979 BlpI GC'TnA_GC Cuts at: 2010 4396 2010 Size: 2386 4593 Bme1580I G_kGCm'C Cuts at: 1790 2492 2865 2995 3023 3302 3702 5040 6196 Size: 702 373 130 28 279 400 1338 1156 Cuts at: 6196 1790 Size: 2573 Fragments arranged by size: 2573 1338 1156 702 400 373 279 130 28 BmgBI CAC'GTC Cuts at: 1318 3853 4042 4164 1318 Size: 2535 189 122 4133 Fragments arranged by size: 4133 2535 189 122 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2859 4710 5668 Size: 416 53 83 399 24 1442 1851 958 Cuts at: 5668 442 Size: 1753 Fragments arranged by size: 1851 1753 1442 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4241 1056 Size: 3185 3794 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2921 3018 3268 3381 3417 5639 1239 Size: 1682 97 250 113 36 2222 2579 Fragments arranged by size: 2579 2222 1682 250 113 97 36 Bpu10I CC'TnA_GC Cuts at: 2559 3233 3899 2559 Size: 674 666 5639 Fragments arranged by size: 5639 674 666 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2552 3365 5104 5969 6267 2552 Size: 813 1739 865 298 3264 Fragments arranged by size: 3264 1739 865 813 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2580 5642 882 Size: 617 1081 3062 2219 Fragments arranged by size: 3062 2219 1081 617 BsaAI yAC'GTr Cuts at: 493 1204 2368 2917 4770 493 Size: 711 1164 549 1853 2702 Fragments arranged by size: 2702 1853 1164 711 549 BsaBI GATnn'nnATC Cuts at: 6973 6973 Size: 6979 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2673 4786 5168 5803 Size: 53 83 186 936 1140 2113 382 635 Cuts at: 5803 275 Size: 1451 Fragments arranged by size: 2113 1451 1140 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5412 5810 6084 6231 6642 5412 Size: 398 274 147 411 5749 Fragments arranged by size: 5749 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2894 2924 4118 4148 1178 Size: 30 165 30 1491 30 1194 30 4009 Fragments arranged by size: 4009 1491 1194 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2666 2975 4180 1186 Size: 1480 309 1205 3985 Fragments arranged by size: 3985 1480 1205 309 BseYI C'CCAG_C Cuts at: 2048 2339 2719 2782 2842 3503 4224 6182 6678 Size: 291 380 63 60 661 721 1958 496 Cuts at: 6678 2048 Size: 2349 Fragments arranged by size: 2349 1958 721 661 496 380 291 63 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2576 2957 3800 1163 Size: 573 840 381 843 4342 Fragments arranged by size: 4342 843 840 573 381 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2548 4429 5190 6218 1540 Size: 275 186 547 1881 761 1028 2301 Fragments arranged by size: 2301 1881 1028 761 547 275 186 BsiHKAI G_wGCw'C Cuts at: 2210 2257 5040 5125 6196 2210 Size: 47 2783 85 1071 2993 Fragments arranged by size: 2993 2783 1071 85 47 BsiWI C'GTAC_G Cuts at: 2055 2055 Size: 6979 BsmI GAATG_Cn' Cuts at: 4490 4583 4490 Size: 93 6886 BsmBI CGTCTCn'nnnn_ Cuts at: 3334 4170 3334 Size: 836 6143 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2186 2210 2257 2473 2492 2856 Size: 399 226 170 24 47 216 19 364 Cuts at: 2856 2865 2995 3023 3066 3302 3624 3702 4298 Size: 9 130 28 43 236 322 78 596 Cuts at: 4298 5040 5125 6196 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 596 399 364 322 236 226 216 170 130 85 78 47 43 28 24 19 9 BspEI T'CCGG_A Cuts at: 6642 6642 Size: 6979 BspHI T'CATG_A Cuts at: 4868 6598 4868 Size: 1730 5249 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2598 4334 844 Size: 1754 1736 3489 Fragments arranged by size: 3489 1754 1736 BsrBI CCG'CTC Cuts at: 1394 1823 3488 3582 4866 1394 Size: 429 1665 94 1284 3507 Fragments arranged by size: 3507 1665 1284 429 94 BsrDI GCAATG_nn' Cuts at: 65 1248 4189 5474 5648 65 Size: 1183 2941 1285 174 1396 Fragments arranged by size: 2941 1396 1285 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4196 5623 5810 1700 Size: 110 2386 1427 187 2869 Fragments arranged by size: 2869 2386 1427 187 110 BsrGI T'GTAC_A Cuts at: 96 4134 96 Size: 4038 2941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6979 BssSI C'ACGA_G Cuts at: 5039 6051 5039 Size: 1012 5967 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6979 BstBI TT'CG_AA Cuts at: 2028 5783 2028 Size: 3755 3224 BstEII G'GTnAC_C Cuts at: 1184 3907 1184 Size: 2723 4256 BstXI CCAn_nnnn'nTGG Cuts at: 3631 4046 3631 Size: 415 6564 BstYI r'GATC_y Cuts at: 1972 2168 2292 2838 3802 4309 4676 5061 5078 Size: 196 124 546 964 507 367 385 17 Cuts at: 5078 6530 6616 6628 6974 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 964 546 507 385 367 346 196 124 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2484 2529 2484 Size: 45 6934 BtgI C'CryG_G Cuts at: 513 1065 2004 3092 3416 4117 4169 513 Size: 552 939 1088 324 701 52 3323 Fragments arranged by size: 3323 1088 939 701 552 324 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3183 487 Size: 683 56 1957 4283 Fragments arranged by size: 4283 1957 683 56 BtsI GCAGTG_nn' Cuts at: 3642 3874 4495 5289 5309 3642 Size: 232 621 794 20 5312 Fragments arranged by size: 5312 794 621 232 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6979 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6944 DraI TTT'AAA Cuts at: 4283 4283 Size: 6979 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2672 5986 809 Size: 507 1356 3314 1802 Fragments arranged by size: 3314 1802 1356 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2545 3958 4263 Size: 54 1425 50 275 18 715 1413 305 Cuts at: 4263 4426 5315 6944 8 Size: 163 889 1629 43 Fragments arranged by size: 1629 1425 1413 889 715 305 275 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2545 4426 1537 Size: 275 733 1881 4090 Fragments arranged by size: 4090 1881 733 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3337 4909 1353 Size: 1984 1572 3423 Fragments arranged by size: 3423 1984 1572 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3658 4065 4139 5566 5940 6086 Size: 1064 138 2069 407 74 1427 374 146 Cuts at: 6086 387 Size: 1280 Fragments arranged by size: 2069 1427 1280 1064 407 374 146 138 74 EcoICRI GAG'CTC Cuts at: 2255 2255 Size: 6979 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2334 2633 2921 3018 3268 3381 3417 Size: 393 702 299 288 97 250 113 36 Cuts at: 3417 3734 3773 5042 5639 6426 6694 1239 Size: 317 39 1269 597 787 268 1524 Fragments arranged by size: 1524 1269 787 702 597 393 317 299 288 268 250 113 97 39 36 EcoNI CCTnn'n_nnAGG Cuts at: 2483 2594 3902 4050 2483 Size: 111 1308 148 5412 Fragments arranged by size: 5412 1308 148 111 EcoO109I rG'GnC_Cy Cuts at: 2426 2488 2489 2525 2991 2992 3019 3020 3115 Size: 62 1 36 466 1 27 1 95 Cuts at: 3115 4020 2426 Size: 905 5385 Fragments arranged by size: 5385 905 466 95 62 36 27 1 1 1 EcoRI G'AATT_C Cuts at: 4288 4666 4288 Size: 378 6601 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4006 4102 4006 Size: 96 6883 FspI TGC'GCA Cuts at: 5485 5485 Size: 6979 HaeII r_GCGC'y Cuts at: 1678 6126 1678 Size: 4448 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2026 2058 2153 2185 2669 Size: 32 135 32 454 32 95 32 484 Cuts at: 2669 2701 2804 2836 4235 4267 5620 5652 5694 Size: 32 103 32 1399 32 1353 32 42 Cuts at: 5694 5726 1373 Size: 32 2626 Fragments arranged by size: 2626 1399 1353 484 454 135 103 95 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3895 4323 4569 669 Size: 988 281 1957 428 246 3079 Fragments arranged by size: 3079 1957 988 428 281 246 HindIII A'AGCT_T Cuts at: 748 3983 748 Size: 3235 3744 HpaI GTT'AAC Cuts at: 4569 4569 Size: 6979 KpnI G_GTAC'C Cuts at: 4304 4304 Size: 6979 MfeI C'AATT_G Cuts at: 4578 4578 Size: 6979 MluI A'CGCG_T Cuts at: 6855 6855 Size: 6979 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2237 3301 3626 6093 6277 1305 Size: 932 1064 325 2467 184 2007 Fragments arranged by size: 2467 2007 1064 932 325 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3960 4265 10 Size: 54 1425 343 2128 305 2724 Fragments arranged by size: 2724 2128 1425 343 305 54 MslI CAynn'nnrTG Cuts at: 518 1166 4208 4937 5296 5455 518 Size: 648 3042 729 359 159 2042 Fragments arranged by size: 3042 2042 729 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2219 2312 2611 2786 3094 3418 3540 4395 Size: 613 93 299 175 308 324 122 855 Cuts at: 4395 5072 6220 6465 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 855 677 613 324 308 299 245 175 122 93 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5488 Fragments arranged by size: 5488 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6979 NheI G'CTAG_C Cuts at: 1052 4237 1052 Size: 3185 3794 NotI GC'GGCC_GC Cuts at: 2545 4426 2545 Size: 1881 5098 NspI r_CATG'y Cuts at: 1132 1229 2464 4337 5882 1132 Size: 97 1235 1873 1545 2229 Fragments arranged by size: 2229 1873 1545 1235 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6979 PciI A'CATG_T Cuts at: 5878 5878 Size: 6979 PflMI CCAn_nnn'nTGG Cuts at: 1108 3956 1108 Size: 2848 4131 PfoI T'CCnGG_A Cuts at: 2400 2400 Size: 6979 PmeI GTTT'AAAC Cuts at: 4283 4283 Size: 6979 PmlI CAC'GTG Cuts at: 2368 2917 2368 Size: 549 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5048 5080 6561 6593 5048 Size: 32 1481 32 5434 Fragments arranged by size: 5434 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2525 3115 4020 2525 Size: 590 905 5484 Fragments arranged by size: 5484 905 590 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6979 PsiI TTA'TAA Cuts at: 4549 4549 Size: 6979 PspOMI G'GGCC_C Cuts at: 2488 2861 2991 3019 2488 Size: 373 130 28 6448 Fragments arranged by size: 6448 373 130 28 PstI C_TGCA'G Cuts at: 830 2151 3687 4331 830 Size: 1321 1536 644 3478 Fragments arranged by size: 3478 1536 1321 644 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6979 PvuII CAG'CTG Cuts at: 2312 2312 Size: 6979 SacI G_AGCT'C Cuts at: 2257 2257 Size: 6979 SacII CC_GC'GG Cuts at: 3095 3419 3095 Size: 324 6655 SalI G'TCGA_C Cuts at: 1936 4321 1936 Size: 2385 4594 SanDI GG'GwC_CC Cuts at: 3115 4020 3115 Size: 905 6074 SbfI CC_TGCA'GG Cuts at: 4331 4331 Size: 6979 ScaI AGT'ACT Cuts at: 5227 6939 5227 Size: 1712 5267 SfcI C'TryA_G Cuts at: 826 1045 2147 3544 3683 4327 5462 6143 6334 Size: 219 1102 1397 139 644 1135 681 191 Cuts at: 6334 826 Size: 1471 Fragments arranged by size: 1471 1397 1135 1102 681 644 219 191 139 SfiI GGCCn_nnn'nGGCC Cuts at: 3444 3444 Size: 6979 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6979 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6979 SmaI CCC'GGG Cuts at: 1872 3017 3617 3858 4306 1872 Size: 1145 600 241 448 4545 Fragments arranged by size: 4545 1145 600 448 241 SmlI C'TyrA_G Cuts at: 820 1017 1943 2567 3380 5083 5984 6246 820 Size: 197 926 624 813 1703 901 262 1553 Fragments arranged by size: 1703 1553 926 901 813 624 262 197 SnaBI TAC'GTA Cuts at: 493 4770 493 Size: 4277 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6979 SphI G_CATG'C Cuts at: 1132 2464 4337 1132 Size: 1332 1873 3774 Fragments arranged by size: 3774 1873 1332 SspI AAT'ATT Cuts at: 5 52 4903 5 Size: 47 4851 2081 Fragments arranged by size: 4851 2081 47 StuI AGG'CCT Cuts at: 1517 2714 3226 1517 Size: 1197 512 5270 Fragments arranged by size: 5270 1197 512 StyI C'CwwG_G Cuts at: 513 1065 2004 2709 4418 513 Size: 552 939 705 1709 3074 Fragments arranged by size: 3074 1709 939 705 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2944 3517 3625 3667 4052 5006 5023 Size: 1255 1364 573 108 42 385 954 17 Cuts at: 5023 5176 5361 5806 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1364 1255 954 573 445 385 185 153 108 42 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4134 5225 6937 Size: 274 80 33 51 1443 2157 1091 1712 Cuts at: 6937 96 Size: 138 Fragments arranged by size: 2157 1712 1443 1091 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3451 3483 6939 6971 3451 Size: 32 3456 32 3459 Fragments arranged by size: 3459 3456 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4001 1316 Size: 2685 4294 XbaI T'CTAG_A Cuts at: 4315 4315 Size: 6979 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3824 4013 4273 3824 Size: 189 260 6530 Fragments arranged by size: 6530 260 189 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6979 XmaI C'CCGG_G Cuts at: 1870 3015 3615 3856 4304 1870 Size: 1145 600 241 448 4545 Fragments arranged by size: 4545 1145 600 448 241 XmnI GAAnn'nnTTC Cuts at: 5108 5108 Size: 6979 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2674 4787 276 Size: 53 83 186 936 1140 2113 2468 Fragments arranged by size: 2468 2113 1140 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SapI SexAI SfoI SrfI SwaI