Restriction map for
pFN21AE2637
(Linear) (Six-base) MAPSORT of: KIEE2637.seq Check: 4669 from: 1 to: 2274
KIEE2637.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AatII G_ACGT'C
Cuts at: 0 671 2274
Size: 671 1603
AccI GT'mk_AC
Cuts at: 0 1877 2210 2274
Size: 1877 333 64
Fragments arranged by size:
1877 333 64
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 628 1729 1768 2274
Size: 329 299 1101 39 506
Fragments arranged by size:
1101 506 329 299 39
AflIII A'CryG_T
Cuts at: 0 1189 1845 2156 2274
Size: 1189 656 311 118
Fragments arranged by size:
1189 656 311 118
AhdI GACnn_n'nnGTC
Cuts at: 0 2244 2274
Size: 2244 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1094 2274
Size: 1094 1180
AlwNI CAG_nnn'CTG
Cuts at: 0 523 2274
Size: 523 1751
ApaI G_GGCC'C
Cuts at: 0 487 860 990 1018 2274
Size: 487 373 130 28 1256
Fragments arranged by size:
1256 487 373 130 28
AseI AT'TA_AT
Cuts at: 0 1784 2274
Size: 1784 490
AvaI C'yCGr_G
Cuts at: 0 1010 1610 1851 2274
Size: 1010 600 241 423
Fragments arranged by size:
1010 600 423 241
BanI G'GyrC_C
Cuts at: 0 787 2274
Size: 787 1487
BanII G_rGCy'C
Cuts at: 0 11 181 252 468 487 851 860 990
Size: 11 170 71 216 19 364 9 130
Cuts at: 990 1018 1061 1619 2274
Size: 28 43 558 655
Fragments arranged by size:
655 558 364 216 170 130 71 43
28 19 11 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 350 665 1652 2274
Size: 350 315 987 622
Fragments arranged by size:
987 622 350 315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2230 2264 2274
Size: 2230 34 10
Fragments arranged by size:
2230 34 10
BglI GCCn_nnn'nGGC
Cuts at: 0 855 1439 2257 2274
Size: 855 584 818 17
Fragments arranged by size:
855 818 584 17
BlpI GC'TnA_GC
Cuts at: 0 5 2274
Size: 5 2269
Bme1580I G_kGCm'C
Cuts at: 0 487 860 990 1018 1297 1697 2274
Size: 487 373 130 28 279 400 577
Fragments arranged by size:
577 487 400 373 279 130 28
BmgBI CAC'GTC
Cuts at: 0 1848 2037 2159 2274
Size: 1848 189 122 115
Fragments arranged by size:
1848 189 122 115
BmrI ACTGGGnnnn_n'
Cuts at: 0 854 2274
Size: 854 1420
BmtI G_CTAG'C
Cuts at: 0 2236 2274
Size: 2236 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 1013 1263 1376 1412 2274
Size: 916 97 250 113 36 862
Fragments arranged by size:
916 862 250 113 97 36
Bpu10I CC'TnA_GC
Cuts at: 0 554 1228 1894 2274
Size: 554 674 666 380
Fragments arranged by size:
674 666 554 380
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1360 2274
Size: 547 813 914
Fragments arranged by size:
914 813 547
BsaI GGTCTCn'nnnn_
Cuts at: 0 575 2274
Size: 575 1699
BsaAI yAC'GTr
Cuts at: 0 363 912 2274
Size: 363 549 1362
Fragments arranged by size:
1362 549 363
BsaHI Gr'CG_yC
Cuts at: 0 668 2274
Size: 668 1606
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 889 919 2113 2143 2274
Size: 889 30 1194 30 131
Fragments arranged by size:
1194 889 131 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 661 970 2175 2274
Size: 661 309 1205 99
Fragments arranged by size:
1205 661 309 99
BseYI C'CCAG_C
Cuts at: 0 43 334 714 777 837 1498 2219 2274
Size: 43 291 380 63 60 661 721 55
Fragments arranged by size:
721 661 380 291 63 60 55 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 952 1795 2274
Size: 571 381 843 479
Fragments arranged by size:
843 571 479 381
BsiEI CG_ry'CG
Cuts at: 0 543 2274
Size: 543 1731
BsiHKAI G_wGCw'C
Cuts at: 0 205 252 2274
Size: 205 47 2022
Fragments arranged by size:
2022 205 47
BsiWI C'GTAC_G
Cuts at: 0 50 2274
Size: 50 2224
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1329 2165 2274
Size: 1329 836 109
Fragments arranged by size:
1329 836 109
Bsp1286I G_dGCh'C
Cuts at: 0 11 181 205 252 468 487 851 860
Size: 11 170 24 47 216 19 364 9
Cuts at: 860 990 1018 1061 1297 1619 1697 2274
Size: 130 28 43 236 322 78 577
Fragments arranged by size:
577 364 322 236 216 170 130 78
47 43 28 24 19 11 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 593 2274
Size: 593 1681
BsrBI CCG'CTC
Cuts at: 0 1483 1577 2274
Size: 1483 94 697
Fragments arranged by size:
1483 697 94
BsrDI GCAATG_nn'
Cuts at: 0 2184 2274
Size: 2184 90
BsrFI r'CCGG_y
Cuts at: 0 2191 2274
Size: 2191 83
BsrGI T'GTAC_A
Cuts at: 0 2129 2274
Size: 2129 145
BstBI TT'CG_AA
Cuts at: 0 23 2274
Size: 23 2251
BstEII G'GTnAC_C
Cuts at: 0 1902 2274
Size: 1902 372
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1626 2041 2274
Size: 1626 415 233
Fragments arranged by size:
1626 415 233
BstYI r'GATC_y
Cuts at: 0 163 287 833 1797 2274
Size: 163 124 546 964 477
Fragments arranged by size:
964 546 477 163 124
Bsu36I CC'TnA_GG
Cuts at: 0 479 524 2274
Size: 479 45 1750
Fragments arranged by size:
1750 479 45
BtgI C'CryG_G
Cuts at: 0 1087 1411 2112 2164 2274
Size: 1087 324 701 52 110
Fragments arranged by size:
1087 701 324 110 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1178 2274
Size: 1178 1096
BtsI GCAGTG_nn'
Cuts at: 0 1637 1869 2274
Size: 1637 232 405
Fragments arranged by size:
1637 405 232
DrdI GACnn_nn'nnGTC
Cuts at: 0 667 2274
Size: 667 1607
EaeI y'GGCC_r
Cuts at: 0 540 1953 2258 2274
Size: 540 1413 305 16
Fragments arranged by size:
1413 540 305 16
EagI C'GGCC_G
Cuts at: 0 540 2274
Size: 540 1734
EarI CTCTTCn'nnn_
Cuts at: 0 1332 2274
Size: 1332 942
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1653 2060 2134 2274
Size: 1653 407 74 140
Fragments arranged by size:
1653 407 140 74
EcoICRI GAG'CTC
Cuts at: 0 250 2274
Size: 250 2024
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 628 916 1013 1263 1376 1412 1729
Size: 329 299 288 97 250 113 36 317
Cuts at: 1729 1768 2274
Size: 39 506
Fragments arranged by size:
506 329 317 299 288 250 113 97
39 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 478 589 1897 2045 2274
Size: 478 111 1308 148 229
Fragments arranged by size:
1308 478 229 148 111
EcoO109I rG'GnC_Cy
Cuts at: 0 421 483 484 520 986 987 1014 1015
Size: 421 62 1 36 466 1 27 1
Cuts at: 1015 1110 2015 2274
Size: 95 905 259
Fragments arranged by size:
905 466 421 259 95 62 36 27
1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2001 2097 2274
Size: 2001 96 177
Fragments arranged by size:
2001 177 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 148 180 664 696 799 831
Size: 21 32 95 32 484 32 103 32
Cuts at: 831 2230 2262 2274
Size: 1399 32 12
Fragments arranged by size:
1399 484 103 95 32 32 32 32
32 21 12
HincII GTy'rAC
Cuts at: 0 1890 2274
Size: 1890 384
HindIII A'AGCT_T
Cuts at: 0 1978 2274
Size: 1978 296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1296 1621 2274
Size: 232 1064 325 653
Fragments arranged by size:
1064 653 325 232
MscI TGG'CCA
Cuts at: 0 1955 2260 2274
Size: 1955 305 14
Fragments arranged by size:
1955 305 14
MslI CAynn'nnrTG
Cuts at: 0 2203 2274
Size: 2203 71
MspA1I CmG'CkG
Cuts at: 0 214 307 606 781 1089 1413 1535 2274
Size: 214 93 299 175 308 324 122 739
Fragments arranged by size:
739 324 308 299 214 175 122 93
NheI G'CTAG_C
Cuts at: 0 2232 2274
Size: 2232 42
NotI GC'GGCC_GC
Cuts at: 0 540 2274
Size: 540 1734
NspI r_CATG'y
Cuts at: 0 459 2274
Size: 459 1815
PflMI CCAn_nnn'nTGG
Cuts at: 0 1951 2274
Size: 1951 323
PfoI T'CCnGG_A
Cuts at: 0 395 2274
Size: 395 1879
PmlI CAC'GTG
Cuts at: 0 363 912 2274
Size: 363 549 1362
Fragments arranged by size:
1362 549 363
PpuMI rG'GwC_Cy
Cuts at: 0 520 1110 2015 2274
Size: 520 590 905 259
Fragments arranged by size:
905 590 520 259
PspOMI G'GGCC_C
Cuts at: 0 483 856 986 1014 2274
Size: 483 373 130 28 1260
Fragments arranged by size:
1260 483 373 130 28
PstI C_TGCA'G
Cuts at: 0 146 1682 2274
Size: 146 1536 592
Fragments arranged by size:
1536 592 146
PvuII CAG'CTG
Cuts at: 0 307 2274
Size: 307 1967
SacI G_AGCT'C
Cuts at: 0 252 2274
Size: 252 2022
SacII CC_GC'GG
Cuts at: 0 1090 1414 2274
Size: 1090 324 860
Fragments arranged by size:
1090 860 324
SanDI GG'GwC_CC
Cuts at: 0 1110 2015 2274
Size: 1110 905 259
Fragments arranged by size:
1110 905 259
SfcI C'TryA_G
Cuts at: 0 142 1539 1678 2274
Size: 142 1397 139 596
Fragments arranged by size:
1397 596 142 139
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1439 2274
Size: 1439 835
SmaI CCC'GGG
Cuts at: 0 1012 1612 1853 2274
Size: 1012 600 241 421
Fragments arranged by size:
1012 600 421 241
SmlI C'TyrA_G
Cuts at: 0 562 1375 2274
Size: 562 813 899
Fragments arranged by size:
899 813 562
SphI G_CATG'C
Cuts at: 0 459 2274
Size: 459 1815
StuI AGG'CCT
Cuts at: 0 709 1221 2274
Size: 709 512 1053
Fragments arranged by size:
1053 709 512
StyI C'CwwG_G
Cuts at: 0 704 2274
Size: 704 1570
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 939 1512 1620 1662 2047 2274
Size: 939 573 108 42 385 227
Fragments arranged by size:
939 573 385 227 108 42
TatI w'GTAC_w
Cuts at: 0 2129 2274
Size: 2129 145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1446 1478 2274
Size: 1446 32 796
Fragments arranged by size:
1446 796 32
Tth111I GACn'n_nGTC
Cuts at: 0 1996 2274
Size: 1996 278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1819 2008 2274
Size: 1819 189 266
Fragments arranged by size:
1819 266 189
XmaI C'CCGG_G
Cuts at: 0 1010 1610 1851 2274
Size: 1010 600 241 423
Fragments arranged by size:
1010 600 423 241
ZraI GAC'GTC
Cuts at: 0 669 2274
Size: 669 1605
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AlfI AlwNI ApaI
AseI AvaI BanI BanII BbsI BcgI BglI BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI Hin4I HincII HindIII MmeI MscI MslI
MspA1I NheI NotI NspI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuII SacI SacII SanDI SfcI SfiI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AleI AloI
ApaLI ApoI AscI AsiSI AvrII BaeI BamHI BbeI
BbvCI BciVI BclI BfrBI BglII BplI BsaBI BsaWI
BsmI BspEI BspHI BssHII BssSI BstAPI BstZ17I ClaI
CspCI DraI DraIII EcoRI EcoRV FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NruI NsiI PacI PasI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmnI