################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI BbeI BbrPI BciVI BfrI BglI BglII BsaHI BseAI BsiWI BsmBI BspEI BsrBI BssHII Bst1107I BstAPI BstZ17I CpoI DraIII DrdI Eco47III EcoRV FseI HaeII Hin1I HpaI KasI KspI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I RsrII SacII SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AocI 2785 CCTCAGG CC^T[ACGT]AGG AviII 2856 TGCGCA TGC^GCA AvrII 1405 CCTAGG C^CTAGG BamHI 2123 GGATCC G^GATCC BbsI 205 GAAGAC GAAGAC(2/6) BclI 465 TGATCA T^GATCA BlnI 1405 CCTAGG C^CTAGG BlpI 586 GCTAAGC GC^T[ACGT]AGC Bpu1102I 586 GCTAAGC GC^T[ACGT]AGC BpuAI 205 GAAGAC GAAGAC(2/6) BsaAI 1670 CACGTA [CT]AC^GT[GA] BspHI 1020 TCATGA T^CATGA BsrFI 2940 GCCGGT [GA]^CCGG[CT] BssSI 4544 CTCGTG CACGAG(-5/-1) BstEII 4014 GGTGACC G^GT[ACGT]ACC BstPI 4014 GGTGACC G^GT[ACGT]ACC BstXI 4362 CCATGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2785 CCTCAGG CC^T[ACGT]AGG CelII 586 GCTAAGC GC^T[ACGT]AGC Cfr10I 2940 GCCGGT [GA]^CCGG[CT] EagI 4574 CGGCCG C^GGCCG EclXI 4574 CGGCCG C^GGCCG Eco52I 4574 CGGCCG C^GGCCG Eco81I 2785 CCTCAGG CC^T[ACGT]AGG EcoO65I 4014 GGTGACC G^GT[ACGT]ACC EcoRI 2491 GAATTC G^AATTC FbaI 465 TGATCA T^GATCA FspI 2856 TGCGCA TGC^GCA MfeI 3302 CAATTG C^AATTG MunI 3302 CAATTG C^AATTG PvuI 1692 CGATCG CGAT^CG RcaI 1020 TCATGA T^CATGA SspI 4176 AATATT AAT^ATT StuI 4627 AGGCCT AGG^CCT XbaI 201 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AflIII 3029 ACATGT A^C[GA][CT]GT 4311 ACATGT AlwNI 596 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG 2962 CAGCAGCTG AseI 883 ATTAAT AT^TAAT 4425 ATTAAT AsnI 883 ATTAAT AT^TAAT 4425 ATTAAT AspI 117 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1556 GACATTGTC AvaI 112 CCCGAG C^[CT]CG[GA]G 130 CCCGAG BcgI 2715 GCACGGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4079 GCATCAGCGTCG BsaBI 541 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2692 GATGCCTATC BsaWI 105 ACCGGA [AT]^CCGG[AT] 1413 ACCGGA BsiEI 1692 CGATCG CG[GA][CT]^CG 4574 CGGCCG BsoBI 112 CCCGAG C^[CT]CG[GA]G 130 CCCGAG BspDI 2689 ATCGAT AT^CGAT 3698 ATCGAT BspLU11I 3029 ACATGT A^CATGT 4311 ACATGT BstBI 1834 TTCGAA TT^CGAA 4180 TTCGAA ClaI 2689 ATCGAT AT^CGAT 3698 ATCGAT DraI 666 TTTAAA TTT^AAA 2163 TTTAAA EcoNI 1658 CCTTGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3406 CCTGTTACAGG EcoT22I 2978 ATGCAT ATGCA^T 3372 ATGCAT FauI 34 CCCGC CCCGC(4/6) 1458 GCGGG MamI 541 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2692 GATGCCTATC NsiI 2978 ATGCAT ATGCA^T 3372 ATGCAT NspV 1834 TTCGAA TT^CGAA 4180 TTCGAA PflFI 117 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1556 GACATTGTC PshBI 883 ATTAAT AT^TAAT 4425 ATTAAT SacI 2407 GAGCTC GAGCT^C 4225 GAGCTC SalI 907 GTCGAC G^TCGAC 4087 GTCGAC SapI 1117 GCTCTTC GCTCTTC(1/4) 2409 GCTCTTC SfuI 1834 TTCGAA TT^CGAA 4180 TTCGAA SpeI 2335 ACTAGT A^CTAGT 2838 ACTAGT Tth111I 117 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1556 GACATTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AfaI AhdI AluI AlwI ApoI Asp700I Asp718I AspEI AspHI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI