(Circular) (Six-base) MAPSORT of: pFN21AE2732.seq Check: 4450 from: 1 to: 9529 pFN21AE2732 9529 bp With 182 enzymes: SgfI * October 12, 2018 18:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4816 6089 4816 Size: 1273 8256 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7339 278 Size: 53 83 186 936 5803 2468 Fragments arranged by size: 5803 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2913 3559 3594 6093 6872 1937 Size: 976 646 35 2499 779 4594 Fragments arranged by size: 4594 2499 976 779 646 35 Acc65I G'GTAC_C Cuts at: 2249 3415 5464 5928 6850 2249 Size: 1166 2049 464 922 4928 Fragments arranged by size: 4928 2049 1166 922 464 AclI AA'CG_TT Cuts at: 1563 7656 8029 1563 Size: 6093 373 3063 Fragments arranged by size: 6093 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2949 3110 3626 3836 3917 4001 4427 4994 Size: 1317 161 516 210 81 84 426 567 Cuts at: 4994 6293 6558 6741 7592 8976 9244 1632 Size: 1299 265 183 851 1384 268 1917 Fragments arranged by size: 1917 1384 1317 1299 851 567 516 426 268 265 210 183 161 84 81 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9332 AflIII A'CryG_T Cuts at: 1203 5034 6316 8428 9405 1203 Size: 3831 1282 2112 977 1327 Fragments arranged by size: 3831 2112 1327 1282 977 AgeI A'CCGG_T Cuts at: 8360 8360 Size: 9529 AhdI GACnn_n'nnGTC Cuts at: 2655 3457 4938 8258 2655 Size: 802 1481 3320 3926 Fragments arranged by size: 3926 3320 1481 802 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9529 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3936 1184 Size: 2752 6777 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3834 3866 5021 5053 3834 Size: 32 1155 32 8310 Fragments arranged by size: 8310 1155 32 32 AlwNI CAG_nnn'CTG Cuts at: 2606 4972 7272 8844 2606 Size: 2366 2300 1572 3291 Fragments arranged by size: 3291 2366 2300 1572 ApaLI G'TGCA_C Cuts at: 7586 8742 7586 Size: 1156 8373 ApoI r'AATT_y Cuts at: 1493 3256 3719 4195 4304 4331 4496 4566 5048 Size: 1763 463 476 109 27 165 70 482 Cuts at: 5048 6838 7064 7216 9325 1493 Size: 1790 226 152 2109 1697 Fragments arranged by size: 2109 1790 1763 1697 482 476 463 226 165 152 109 70 27 AseI AT'TA_AT Cuts at: 160 1032 2889 6431 8083 160 Size: 872 1857 3542 1652 1606 Fragments arranged by size: 3542 1857 1652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9529 AvaI C'yCGr_G Cuts at: 1870 1943 2117 2135 6854 1870 Size: 73 174 18 4719 4545 Fragments arranged by size: 4719 4545 174 73 18 AvrII C'CTAG_G Cuts at: 3410 3410 Size: 9529 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2952 2985 2952 Size: 33 9496 BamHI G'GATC_C Cuts at: 4128 6859 7226 4128 Size: 2731 367 6431 Fragments arranged by size: 6431 2731 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2249 3415 4757 5464 5928 Size: 325 217 627 462 1166 1342 707 464 Cuts at: 5928 6401 6532 6850 8305 618 Size: 473 131 318 1455 1842 Fragments arranged by size: 1842 1455 1342 1166 707 627 473 464 462 325 318 217 131 BanII G_rGCy'C Cuts at: 1391 4405 4416 4735 6234 6848 1391 Size: 3014 11 319 1499 614 4072 Fragments arranged by size: 4072 3014 1499 614 319 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2217 928 Size: 971 318 8240 Fragments arranged by size: 8240 971 318 BbvCI CC'TCA_GC Cuts at: 2570 4614 5058 2570 Size: 2044 444 7041 Fragments arranged by size: 7041 2044 444 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4709 4743 6073 6107 7720 7754 1903 Size: 34 2772 34 1330 34 1613 34 3678 Fragments arranged by size: 3678 2772 1613 1330 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7421 8631 7421 Size: 1210 8319 BclI T'GATC_A Cuts at: 1548 2470 1548 Size: 922 8607 BfrBI ATG'CAT Cuts at: 4985 5379 4985 Size: 394 9135 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8140 136 Size: 107 122 71 631 605 6468 1525 Fragments arranged by size: 6468 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 9524 9524 Size: 9529 BlpI GC'TnA_GC Cuts at: 2592 6946 2592 Size: 4354 5175 Bme1580I G_kGCm'C Cuts at: 1790 2168 2613 3239 3676 4760 5668 7590 8746 Size: 378 445 626 437 1084 908 1922 1156 Cuts at: 8746 1790 Size: 2573 Fragments arranged by size: 2573 1922 1156 1084 908 626 445 437 378 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9529 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4508 6449 7260 Size: 416 53 83 399 24 3091 1941 811 Cuts at: 7260 8218 442 Size: 958 1753 Fragments arranged by size: 3091 1941 1753 958 811 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9529 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3556 3945 4055 5499 6610 8189 1239 Size: 2317 389 110 1444 1111 1579 2579 Fragments arranged by size: 2579 2317 1579 1444 1111 389 110 Bpu10I CC'TnA_GC Cuts at: 2570 4614 5058 2570 Size: 2044 444 7041 Fragments arranged by size: 7041 2044 444 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3506 3961 4137 5268 5371 5740 6031 6770 7654 Size: 455 176 1131 103 369 291 739 884 Cuts at: 7654 8519 8817 3506 Size: 865 298 4218 Fragments arranged by size: 4218 1131 884 865 739 455 369 298 291 176 103 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2114 3055 3910 4515 8192 882 Size: 617 615 941 855 605 3677 2219 Fragments arranged by size: 3677 2219 941 855 617 615 605 BsaAI yAC'GTr Cuts at: 493 1204 3677 7320 493 Size: 711 2473 3643 2702 Fragments arranged by size: 3643 2702 2473 711 BsaBI GATnn'nnATC Cuts at: 2550 4701 9523 2550 Size: 2151 4822 2556 Fragments arranged by size: 4822 2556 2151 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7336 7718 8353 275 Size: 53 83 186 936 5803 382 635 1451 Fragments arranged by size: 5803 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2110 3418 7962 8360 8634 8781 9192 2110 Size: 1308 4544 398 274 147 411 2447 Fragments arranged by size: 4544 2447 1308 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 9304 Fragments arranged by size: 9304 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2025 2087 3797 3824 4245 4631 5783 5811 Size: 839 62 1710 27 421 386 1152 28 Cuts at: 5811 5956 6779 1186 Size: 145 823 3936 Fragments arranged by size: 3936 1710 1152 839 823 421 386 145 62 28 27 BseYI C'CCAG_C Cuts at: 2261 3519 4752 5618 6301 8732 9228 2261 Size: 1258 1233 866 683 2431 496 2562 Fragments arranged by size: 2562 2431 1258 1233 866 683 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2616 3194 3773 1163 Size: 573 880 578 579 6919 Fragments arranged by size: 6919 880 579 578 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3700 6582 6979 7740 8768 1540 Size: 275 186 1699 2882 397 761 1028 2301 Fragments arranged by size: 2882 2301 1699 1028 761 397 275 186 BsiHKAI G_wGCw'C Cuts at: 2495 3529 3542 4416 6234 6654 7590 7675 8746 Size: 1034 13 874 1818 420 936 85 1071 Cuts at: 8746 2495 Size: 3278 Fragments arranged by size: 3278 1818 1071 1034 936 874 420 85 13 BsmI GAATG_Cn' Cuts at: 2362 4278 5381 7040 7133 2362 Size: 1916 1103 1659 93 4758 Fragments arranged by size: 4758 1916 1659 1103 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2168 2495 2613 3239 3529 3542 3676 Size: 399 378 327 118 626 290 13 134 Cuts at: 3676 4405 4416 4735 4760 5668 6234 6654 6848 Size: 729 11 319 25 908 566 420 194 Cuts at: 6848 7590 7675 8746 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 908 742 729 626 566 420 399 378 327 319 290 194 134 118 85 25 13 11 BspEI T'CCGG_A Cuts at: 9192 9192 Size: 9529 BspHI T'CATG_A Cuts at: 3025 7418 9148 3025 Size: 4393 1730 3406 Fragments arranged by size: 4393 3406 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2638 3052 3853 3928 4816 5323 6089 6884 Size: 1794 414 801 75 888 507 766 795 Cuts at: 6884 844 Size: 3489 Fragments arranged by size: 3489 1794 888 801 795 766 507 414 75 BsrBI CCG'CTC Cuts at: 1394 1823 7416 1394 Size: 429 5593 3507 Fragments arranged by size: 5593 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2593 3113 4263 5017 5115 6348 8024 Size: 1183 1345 520 1150 754 98 1233 1676 Cuts at: 8024 8198 65 Size: 174 1396 Fragments arranged by size: 1676 1396 1345 1233 1183 1150 754 520 174 98 BsrFI r'CCGG_y Cuts at: 1700 1810 4945 8173 8360 1700 Size: 110 3135 3228 187 2869 Fragments arranged by size: 3228 3135 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2485 6075 6249 96 Size: 2389 3590 174 3376 Fragments arranged by size: 3590 3376 2389 174 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9529 BssSI C'ACGA_G Cuts at: 6549 7589 8601 6549 Size: 1040 1012 7477 Fragments arranged by size: 7477 1040 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9529 BstBI TT'CG_AA Cuts at: 3840 6186 8333 3840 Size: 2346 2147 5036 Fragments arranged by size: 5036 2346 2147 BstEII G'GTnAC_C Cuts at: 1184 6019 1184 Size: 4835 4694 BstXI CCAn_nnnn'nTGG Cuts at: 6374 6374 Size: 9529 BstYI r'GATC_y Cuts at: 1972 2439 2519 2551 3727 4081 4128 6859 7226 Size: 467 80 32 1176 354 47 2731 367 Cuts at: 7226 7611 7628 9080 9166 9178 9524 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 2731 1977 1452 1176 467 385 367 354 346 86 80 47 32 17 12 Bsu36I CC'TnA_GG Cuts at: 4791 4791 Size: 9529 BtgI C'CryG_G Cuts at: 513 1065 2067 3822 5560 5763 6568 513 Size: 552 1002 1755 1738 203 805 3474 Fragments arranged by size: 3474 1755 1738 1002 805 552 203 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4143 4936 6285 487 Size: 683 56 2917 793 1349 3731 Fragments arranged by size: 3731 2917 1349 793 683 56 BtsI GCAGTG_nn' Cuts at: 2984 3799 4363 5668 6742 7045 7839 7859 2984 Size: 815 564 1305 1074 303 794 20 4654 Fragments arranged by size: 4654 1305 1074 815 794 564 303 20 ClaI AT'CG_AT Cuts at: 1554 4695 5704 1554 Size: 3141 1009 5379 Fragments arranged by size: 5379 3141 1009 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9494 DraI TTT'AAA Cuts at: 2673 4170 6833 2673 Size: 1497 2663 5369 Fragments arranged by size: 5369 2663 1497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8536 809 Size: 507 7220 1802 Fragments arranged by size: 7220 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3275 5383 6111 Size: 54 1425 50 275 18 1445 2108 728 Cuts at: 6111 6579 6976 7865 9494 8 Size: 468 397 889 1629 43 Fragments arranged by size: 2108 1629 1445 1425 889 728 468 397 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6579 6976 1537 Size: 275 4767 397 4090 Fragments arranged by size: 4767 4090 397 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3129 4071 4421 4616 6282 7459 1353 Size: 1776 942 350 195 1666 1177 3423 Fragments arranged by size: 3423 1776 1666 1177 942 350 195 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8116 8490 8636 387 Size: 1064 138 6527 374 146 1280 Fragments arranged by size: 6527 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4414 6232 4414 Size: 1818 7711 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2949 3110 3556 3626 3836 3917 3945 Size: 393 1317 161 446 70 210 81 28 Cuts at: 3945 4001 4055 4427 4994 5499 6293 6558 6610 Size: 56 54 372 567 505 794 265 52 Cuts at: 6610 6741 7592 8189 8976 9244 1239 Size: 131 851 597 787 268 1524 Fragments arranged by size: 1524 1317 851 794 787 597 567 505 446 393 372 268 265 210 161 131 81 70 56 54 52 28 EcoNI CCTnn'n_nnAGG Cuts at: 3667 5415 3667 Size: 1748 7781 EcoO109I rG'GnC_Cy Cuts at: 3976 4477 5739 5879 3976 Size: 501 1262 140 7626 Fragments arranged by size: 7626 1262 501 140 EcoRI G'AATT_C Cuts at: 4496 6838 7216 4496 Size: 2342 378 6809 Fragments arranged by size: 6809 2342 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4432 6040 4432 Size: 1608 7921 FspI TGC'GCA Cuts at: 4863 8035 4863 Size: 3172 6357 HaeII r_GCGC'y Cuts at: 1678 8676 1678 Size: 6998 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 2641 2673 3443 Size: 32 135 32 539 32 498 32 770 Cuts at: 3443 3458 3475 3490 4919 4924 4951 4956 8170 Size: 15 17 15 1429 5 27 5 3214 Cuts at: 8170 8202 8244 8276 1373 Size: 32 42 32 2626 Fragments arranged by size: 3214 2626 1429 770 539 498 135 42 32 32 32 32 32 32 27 17 15 15 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 2292 2914 4807 6094 6873 7119 Size: 988 281 354 622 1893 1287 779 246 Cuts at: 7119 669 Size: 3079 Fragments arranged by size: 3079 1893 1287 988 779 622 354 281 246 HindIII A'AGCT_T Cuts at: 748 2594 2705 3482 4250 4884 4959 5206 748 Size: 1846 111 777 768 634 75 247 5071 Fragments arranged by size: 5071 1846 777 768 634 247 111 75 HpaI GTT'AAC Cuts at: 7119 7119 Size: 9529 KpnI G_GTAC'C Cuts at: 2253 3419 5468 5932 6854 2253 Size: 1166 2049 464 922 4928 Fragments arranged by size: 4928 2049 1166 922 464 MfeI C'AATT_G Cuts at: 5307 7128 5307 Size: 1821 7708 MluI A'CGCG_T Cuts at: 9405 9405 Size: 9529 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3061 3345 4389 5571 8643 8827 1305 Size: 1756 284 1044 1182 3072 184 2007 Fragments arranged by size: 3072 2007 1756 1182 1044 284 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3277 5385 6113 10 Size: 54 1425 343 1445 2108 728 3426 Fragments arranged by size: 3426 2108 1445 1425 728 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4826 6257 6372 6573 6614 6645 7487 Size: 648 3660 1431 115 201 41 31 842 Cuts at: 7487 7846 8005 518 Size: 359 159 2042 Fragments arranged by size: 3660 2042 1431 842 648 359 201 159 115 41 31 MspA1I CmG'CkG Cuts at: 1606 2018 3955 4652 4752 4972 6945 7622 8770 Size: 412 1937 697 100 220 1973 677 1148 Cuts at: 8770 9015 1606 Size: 245 2120 Fragments arranged by size: 2120 1973 1937 1148 697 677 412 245 220 100 NcoI C'CATG_G Cuts at: 513 1065 2067 3822 5560 6568 513 Size: 552 1002 1755 1738 1008 3474 Fragments arranged by size: 3474 1755 1738 1008 1002 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9529 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9529 NotI GC'GGCC_GC Cuts at: 6976 6976 Size: 9529 NsiI A_TGCA'T Cuts at: 4987 5381 4987 Size: 394 9135 NspI r_CATG'y Cuts at: 1132 1229 2290 2822 4825 5038 5542 6320 6887 Size: 97 1061 532 2003 213 504 778 567 Cuts at: 6887 8432 1132 Size: 1545 2229 Fragments arranged by size: 2229 2003 1545 1061 778 567 532 504 213 97 PasI CC'CwG_GG Cuts at: 1348 6511 1348 Size: 5163 4366 PciI A'CATG_T Cuts at: 5034 6316 8428 5034 Size: 1282 2112 6135 Fragments arranged by size: 6135 2112 1282 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9529 PfoI T'CCnGG_A Cuts at: 2992 3162 4527 2992 Size: 170 1365 7994 Fragments arranged by size: 7994 1365 170 PmeI GTTT'AAAC Cuts at: 6833 6833 Size: 9529 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3921 3953 7598 7630 9111 9143 3921 Size: 32 3645 32 1481 32 4307 Fragments arranged by size: 4307 3645 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3976 4477 5739 5879 3976 Size: 501 1262 140 7626 Fragments arranged by size: 7626 1262 501 140 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9529 PsiI TTA'TAA Cuts at: 7099 7099 Size: 9529 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3998 4030 3998 Size: 32 9497 PstI C_TGCA'G Cuts at: 830 4026 4657 6169 6881 830 Size: 3196 631 1512 712 3478 Fragments arranged by size: 3478 3196 1512 712 631 PvuI CG_AT'CG Cuts at: 2001 3700 2001 Size: 1699 7830 PvuII CAG'CTG Cuts at: 3955 4652 4752 4972 3955 Size: 697 100 220 8512 Fragments arranged by size: 8512 697 220 100 SacI G_AGCT'C Cuts at: 4416 6234 4416 Size: 1818 7711 SalI G'TCGA_C Cuts at: 1936 2912 6092 6871 1936 Size: 976 3180 779 4594 Fragments arranged by size: 4594 3180 976 779 SapI GCTCTTCn'nnn_ Cuts at: 3129 4421 3129 Size: 1292 8237 SbfI CC_TGCA'GG Cuts at: 6881 6881 Size: 9529 ScaI AGT'ACT Cuts at: 4007 5096 6266 7777 9489 4007 Size: 1089 1170 1511 1712 4047 Fragments arranged by size: 4047 1712 1511 1170 1089 SfcI C'TryA_G Cuts at: 826 1045 2581 2619 4022 4139 4287 4653 6165 Size: 219 1536 38 1403 117 148 366 1512 Cuts at: 6165 6877 8012 8693 8884 826 Size: 712 1135 681 191 1471 Fragments arranged by size: 1536 1512 1471 1403 1135 712 681 366 219 191 148 117 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9529 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9529 SmaI CCC'GGG Cuts at: 1872 6856 1872 Size: 4984 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3485 3940 4152 5283 5350 5755 Size: 197 926 1542 455 212 1131 67 405 Cuts at: 5755 6010 6749 7633 8534 8796 820 Size: 255 739 884 901 262 1553 Fragments arranged by size: 1553 1542 1131 926 901 884 739 455 405 262 255 212 197 67 SnaBI TAC'GTA Cuts at: 493 7320 493 Size: 6827 2702 SpeI A'CTAG_T Cuts at: 152 4340 4843 152 Size: 4188 503 4838 Fragments arranged by size: 4838 4188 503 SphI G_CATG'C Cuts at: 1132 2822 4825 5542 6887 1132 Size: 1690 2003 717 1345 3774 Fragments arranged by size: 3774 2003 1690 1345 717 SspI AAT'ATT Cuts at: 5 52 6183 7453 5 Size: 47 6131 1270 2081 Fragments arranged by size: 6131 2081 1270 47 StuI AGG'CCT Cuts at: 1517 6634 1517 Size: 5117 4412 StyI C'CwwG_G Cuts at: 513 1065 2067 2575 3410 3822 5560 6568 6635 Size: 552 1002 508 835 412 1738 1008 67 Cuts at: 6635 6968 513 Size: 333 3074 Fragments arranged by size: 3074 1738 1008 1002 835 552 508 412 333 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2138 2322 2504 7556 7573 7726 7911 Size: 1255 558 184 182 5052 17 153 185 Cuts at: 7911 8356 325 Size: 445 1498 Fragments arranged by size: 5052 1498 1255 558 445 185 184 182 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2485 3090 4005 Size: 274 80 33 51 1443 508 605 915 Cuts at: 4005 5094 6075 6249 6264 7775 9487 96 Size: 1089 981 174 15 1511 1712 138 Fragments arranged by size: 1712 1511 1443 1089 981 915 605 508 274 174 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2382 2414 9489 9521 2382 Size: 32 7075 32 2390 Fragments arranged by size: 7075 2390 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2125 3564 1316 Size: 809 1439 7281 Fragments arranged by size: 7281 1439 809 XbaI T'CTAG_A Cuts at: 2206 6865 2206 Size: 4659 4870 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3732 5023 6488 6768 3732 Size: 1291 1465 280 6493 Fragments arranged by size: 6493 1465 1291 280 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9529 XmaI C'CCGG_G Cuts at: 1870 6854 1870 Size: 4984 4545 XmnI GAAnn'nnTTC Cuts at: 4253 5154 6182 7658 4253 Size: 901 1028 1476 6124 Fragments arranged by size: 6124 1476 1028 901 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7337 276 Size: 53 83 186 936 5803 2468 Fragments arranged by size: 5803 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI