(Linear) (Six-base) MAPSORT of: KIEE2732.seq Check: 3651 from: 1 to: 4824 KIEE2732.orf With 182 enzymes: SgfI * October 2, 2018 18:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2811 4084 4824 Size: 2811 1273 740 Fragments arranged by size: 2811 1273 740 AccI GT'mk_AC Cuts at: 0 908 1554 1589 4088 4824 Size: 908 646 35 2499 736 Fragments arranged by size: 2499 908 736 646 35 Acc65I G'GTAC_C Cuts at: 0 244 1410 3459 3923 4824 Size: 244 1166 2049 464 901 Fragments arranged by size: 2049 1166 901 464 244 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 944 1105 1621 1831 1912 1996 2422 2989 Size: 944 161 516 210 81 84 426 567 Cuts at: 2989 4288 4553 4736 4824 Size: 1299 265 183 88 Fragments arranged by size: 1299 944 567 516 426 265 210 183 161 88 84 81 AflIII A'CryG_T Cuts at: 0 3029 4311 4824 Size: 3029 1282 513 Fragments arranged by size: 3029 1282 513 AhdI GACnn_n'nnGTC Cuts at: 0 650 1452 2933 4824 Size: 650 802 1481 1891 Fragments arranged by size: 1891 1481 802 650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1931 4824 Size: 1931 2893 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1829 1861 3016 3048 4824 Size: 1829 32 1155 32 1776 Fragments arranged by size: 1829 1776 1155 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 601 2967 4824 Size: 601 2366 1857 Fragments arranged by size: 2366 1857 601 ApoI r'AATT_y Cuts at: 0 1251 1714 2190 2299 2326 2491 2561 3043 Size: 1251 463 476 109 27 165 70 482 Cuts at: 3043 4824 Size: 1781 Fragments arranged by size: 1781 1251 482 476 463 165 109 70 27 AseI AT'TA_AT Cuts at: 0 884 4426 4824 Size: 884 3542 398 Fragments arranged by size: 3542 884 398 AvaI C'yCGr_G Cuts at: 0 112 130 4824 Size: 112 18 4694 Fragments arranged by size: 4694 112 18 AvrII C'CTAG_G Cuts at: 0 1405 4824 Size: 1405 3419 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 947 980 4824 Size: 947 33 3844 Fragments arranged by size: 3844 947 33 BamHI G'GATC_C Cuts at: 0 2123 4824 Size: 2123 2701 BanI G'GyrC_C Cuts at: 0 244 1410 2752 3459 3923 4396 4527 4824 Size: 244 1166 1342 707 464 473 131 297 Fragments arranged by size: 1342 1166 707 473 464 297 244 131 BanII G_rGCy'C Cuts at: 0 2400 2411 2730 4229 4824 Size: 2400 11 319 1499 595 Fragments arranged by size: 2400 1499 595 319 11 BbsI GAAGACnn'nnnn_ Cuts at: 0 212 4824 Size: 212 4612 BbvCI CC'TCA_GC Cuts at: 0 565 2609 3053 4824 Size: 565 2044 444 1771 Fragments arranged by size: 2044 1771 565 444 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2704 2738 4068 4102 4824 Size: 2704 34 1330 34 722 Fragments arranged by size: 2704 1330 722 34 34 BclI T'GATC_A Cuts at: 0 465 4824 Size: 465 4359 BfrBI ATG'CAT Cuts at: 0 2980 3374 4824 Size: 2980 394 1450 Fragments arranged by size: 2980 1450 394 BlpI GC'TnA_GC Cuts at: 0 587 4824 Size: 587 4237 Bme1580I G_kGCm'C Cuts at: 0 163 608 1234 1671 2755 3663 4824 Size: 163 445 626 437 1084 908 1161 Fragments arranged by size: 1161 1084 908 626 445 437 163 BmrI ACTGGGnnnn_n' Cuts at: 0 2503 4444 4824 Size: 2503 1941 380 Fragments arranged by size: 2503 1941 380 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1551 1940 2050 3494 4605 4824 Size: 1551 389 110 1444 1111 219 Fragments arranged by size: 1551 1444 1111 389 219 110 Bpu10I CC'TnA_GC Cuts at: 0 565 2609 3053 4824 Size: 565 2044 444 1771 Fragments arranged by size: 2044 1771 565 444 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1501 1956 2132 3263 3366 3735 4026 4765 Size: 1501 455 176 1131 103 369 291 739 Cuts at: 4765 4824 Size: 59 Fragments arranged by size: 1501 1131 739 455 369 291 176 103 59 BsaI GGTCTCn'nnnn_ Cuts at: 0 109 1050 1905 2510 4824 Size: 109 941 855 605 2314 Fragments arranged by size: 2314 941 855 605 109 BsaAI yAC'GTr Cuts at: 0 1672 4824 Size: 1672 3152 BsaBI GATnn'nnATC Cuts at: 0 545 2696 4824 Size: 545 2151 2128 Fragments arranged by size: 2151 2128 545 BsaWI w'CCGG_w Cuts at: 0 105 1413 4824 Size: 105 1308 3411 Fragments arranged by size: 3411 1308 105 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 20 82 1792 1819 2240 2626 3778 3806 Size: 20 62 1710 27 421 386 1152 28 Cuts at: 3806 3951 4774 4824 Size: 145 823 50 Fragments arranged by size: 1710 1152 823 421 386 145 62 50 28 27 20 BseYI C'CCAG_C Cuts at: 0 256 1514 2747 3613 4296 4824 Size: 256 1258 1233 866 683 528 Fragments arranged by size: 1258 1233 866 683 528 256 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 611 1189 1768 4824 Size: 611 578 579 3056 Fragments arranged by size: 3056 611 579 578 BsiEI CG_ry'CG Cuts at: 0 1695 4577 4824 Size: 1695 2882 247 Fragments arranged by size: 2882 1695 247 BsiHKAI G_wGCw'C Cuts at: 0 490 1524 1537 2411 4229 4649 4824 Size: 490 1034 13 874 1818 420 175 Fragments arranged by size: 1818 1034 874 490 420 175 13 BsmI GAATG_Cn' Cuts at: 0 357 2273 3376 4824 Size: 357 1916 1103 1448 Fragments arranged by size: 1916 1448 1103 357 Bsp1286I G_dGCh'C Cuts at: 0 163 490 608 1234 1524 1537 1671 2400 Size: 163 327 118 626 290 13 134 729 Cuts at: 2400 2411 2730 2755 3663 4229 4649 4824 Size: 11 319 25 908 566 420 175 Fragments arranged by size: 908 729 626 566 420 327 319 290 175 163 134 118 25 13 11 BspHI T'CATG_A Cuts at: 0 1020 4824 Size: 1020 3804 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 633 1047 1848 1923 2811 3318 4084 4824 Size: 633 414 801 75 888 507 766 740 Fragments arranged by size: 888 801 766 740 633 507 414 75 BsrDI GCAATG_nn' Cuts at: 0 588 1108 2258 3012 3110 4343 4824 Size: 588 520 1150 754 98 1233 481 Fragments arranged by size: 1233 1150 754 588 520 481 98 BsrFI r'CCGG_y Cuts at: 0 2940 4824 Size: 2940 1884 BsrGI T'GTAC_A Cuts at: 0 480 4070 4244 4824 Size: 480 3590 174 580 Fragments arranged by size: 3590 580 480 174 BssSI C'ACGA_G Cuts at: 0 4544 4824 Size: 4544 280 BstBI TT'CG_AA Cuts at: 0 1835 4181 4824 Size: 1835 2346 643 Fragments arranged by size: 2346 1835 643 BstEII G'GTnAC_C Cuts at: 0 4014 4824 Size: 4014 810 BstXI CCAn_nnnn'nTGG Cuts at: 0 4369 4824 Size: 4369 455 BstYI r'GATC_y Cuts at: 0 434 514 546 1722 2076 2123 4824 Size: 434 80 32 1176 354 47 2701 Fragments arranged by size: 2701 1176 434 354 80 47 32 Bsu36I CC'TnA_GG Cuts at: 0 2786 4824 Size: 2786 2038 BtgI C'CryG_G Cuts at: 0 62 1817 3555 3758 4563 4824 Size: 62 1755 1738 203 805 261 Fragments arranged by size: 1755 1738 805 261 203 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2138 2931 4280 4824 Size: 2138 793 1349 544 Fragments arranged by size: 2138 1349 793 544 BtsI GCAGTG_nn' Cuts at: 0 979 1794 2358 3663 4737 4824 Size: 979 815 564 1305 1074 87 Fragments arranged by size: 1305 1074 979 815 564 87 ClaI AT'CG_AT Cuts at: 0 2690 3699 4824 Size: 2690 1009 1125 Fragments arranged by size: 2690 1125 1009 DraI TTT'AAA Cuts at: 0 668 2165 4824 Size: 668 1497 2659 Fragments arranged by size: 2659 1497 668 EaeI y'GGCC_r Cuts at: 0 1270 3378 4106 4574 4824 Size: 1270 2108 728 468 250 Fragments arranged by size: 2108 1270 728 468 250 EagI C'GGCC_G Cuts at: 0 4574 4824 Size: 4574 250 EarI CTCTTCn'nnn_ Cuts at: 0 1124 2066 2416 2611 4277 4824 Size: 1124 942 350 195 1666 547 Fragments arranged by size: 1666 1124 942 547 350 195 EcoICRI GAG'CTC Cuts at: 0 2409 4227 4824 Size: 2409 1818 597 Fragments arranged by size: 2409 1818 597 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 944 1105 1551 1621 1831 1912 1940 1996 Size: 944 161 446 70 210 81 28 56 Cuts at: 1996 2050 2422 2989 3494 4288 4553 4605 4736 Size: 54 372 567 505 794 265 52 131 Cuts at: 4736 4824 Size: 88 Fragments arranged by size: 944 794 567 505 446 372 265 210 161 131 88 81 70 56 54 52 28 EcoNI CCTnn'n_nnAGG Cuts at: 0 1662 3410 4824 Size: 1662 1748 1414 Fragments arranged by size: 1748 1662 1414 EcoO109I rG'GnC_Cy Cuts at: 0 1971 2472 3734 3874 4824 Size: 1971 501 1262 140 950 Fragments arranged by size: 1971 1262 950 501 140 EcoRI G'AATT_C Cuts at: 0 2491 4824 Size: 2491 2333 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2427 4035 4824 Size: 2427 1608 789 Fragments arranged by size: 2427 1608 789 FspI TGC'GCA Cuts at: 0 2858 4824 Size: 2858 1966 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 106 138 636 668 1438 1453 1470 1485 Size: 106 32 498 32 770 15 17 15 Cuts at: 1485 2914 2919 2946 2951 4824 Size: 1429 5 27 5 1873 Fragments arranged by size: 1873 1429 770 498 106 32 32 27 17 15 15 5 5 HincII GTy'rAC Cuts at: 0 287 909 2802 4089 4824 Size: 287 622 1893 1287 735 Fragments arranged by size: 1893 1287 735 622 287 HindIII A'AGCT_T Cuts at: 0 589 700 1477 2245 2879 2954 3201 4824 Size: 589 111 777 768 634 75 247 1623 Fragments arranged by size: 1623 777 768 634 589 247 111 75 KpnI G_GTAC'C Cuts at: 0 248 1414 3463 3927 4824 Size: 248 1166 2049 464 897 Fragments arranged by size: 2049 1166 897 464 248 MfeI C'AATT_G Cuts at: 0 3302 4824 Size: 3302 1522 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1056 1340 2384 3566 4824 Size: 1056 284 1044 1182 1258 Fragments arranged by size: 1258 1182 1056 1044 284 MscI TGG'CCA Cuts at: 0 1272 3380 4108 4824 Size: 1272 2108 728 716 Fragments arranged by size: 2108 1272 728 716 MslI CAynn'nnrTG Cuts at: 0 2821 4252 4367 4568 4609 4640 4824 Size: 2821 1431 115 201 41 31 184 Fragments arranged by size: 2821 1431 201 184 115 41 31 MspA1I CmG'CkG Cuts at: 0 13 1950 2647 2747 2967 4824 Size: 13 1937 697 100 220 1857 Fragments arranged by size: 1937 1857 697 220 100 13 NcoI C'CATG_G Cuts at: 0 62 1817 3555 4563 4824 Size: 62 1755 1738 1008 261 Fragments arranged by size: 1755 1738 1008 261 62 NsiI A_TGCA'T Cuts at: 0 2982 3376 4824 Size: 2982 394 1448 Fragments arranged by size: 2982 1448 394 NspI r_CATG'y Cuts at: 0 285 817 2820 3033 3537 4315 4824 Size: 285 532 2003 213 504 778 509 Fragments arranged by size: 2003 778 532 509 504 285 213 PasI CC'CwG_GG Cuts at: 0 4506 4824 Size: 4506 318 PciI A'CATG_T Cuts at: 0 3029 4311 4824 Size: 3029 1282 513 Fragments arranged by size: 3029 1282 513 PfoI T'CCnGG_A Cuts at: 0 987 1157 2522 4824 Size: 987 170 1365 2302 Fragments arranged by size: 2302 1365 987 170 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1916 1948 4824 Size: 1916 32 2876 Fragments arranged by size: 2876 1916 32 PpuMI rG'GwC_Cy Cuts at: 0 1971 2472 3734 3874 4824 Size: 1971 501 1262 140 950 Fragments arranged by size: 1971 1262 950 501 140 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1993 2025 4824 Size: 1993 32 2799 Fragments arranged by size: 2799 1993 32 PstI C_TGCA'G Cuts at: 0 2021 2652 4164 4824 Size: 2021 631 1512 660 Fragments arranged by size: 2021 1512 660 631 PvuI CG_AT'CG Cuts at: 0 1695 4824 Size: 1695 3129 PvuII CAG'CTG Cuts at: 0 1950 2647 2747 2967 4824 Size: 1950 697 100 220 1857 Fragments arranged by size: 1950 1857 697 220 100 SacI G_AGCT'C Cuts at: 0 2411 4229 4824 Size: 2411 1818 595 Fragments arranged by size: 2411 1818 595 SalI G'TCGA_C Cuts at: 0 907 4087 4824 Size: 907 3180 737 Fragments arranged by size: 3180 907 737 SapI GCTCTTCn'nnn_ Cuts at: 0 1124 2416 4824 Size: 1124 1292 2408 Fragments arranged by size: 2408 1292 1124 ScaI AGT'ACT Cuts at: 0 2002 3091 4261 4824 Size: 2002 1089 1170 563 Fragments arranged by size: 2002 1170 1089 563 SfcI C'TryA_G Cuts at: 0 576 614 2017 2134 2282 2648 4160 4824 Size: 576 38 1403 117 148 366 1512 664 Fragments arranged by size: 1512 1403 664 576 366 148 117 38 SmlI C'TyrA_G Cuts at: 0 1480 1935 2147 3278 3345 3750 4005 4744 Size: 1480 455 212 1131 67 405 255 739 Cuts at: 4744 4824 Size: 80 Fragments arranged by size: 1480 1131 739 455 405 255 212 80 67 SpeI A'CTAG_T Cuts at: 0 2335 2838 4824 Size: 2335 503 1986 Fragments arranged by size: 2335 1986 503 SphI G_CATG'C Cuts at: 0 817 2820 3537 4824 Size: 817 2003 717 1287 Fragments arranged by size: 2003 1287 817 717 SspI AAT'ATT Cuts at: 0 4178 4824 Size: 4178 646 StuI AGG'CCT Cuts at: 0 4629 4824 Size: 4629 195 StyI C'CwwG_G Cuts at: 0 62 570 1405 1817 3555 4563 4630 4824 Size: 62 508 835 412 1738 1008 67 194 Fragments arranged by size: 1738 1008 835 508 412 194 67 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 133 317 499 4824 Size: 133 184 182 4325 Fragments arranged by size: 4325 184 182 133 TatI w'GTAC_w Cuts at: 0 480 1085 2000 3089 4070 4244 4259 4824 Size: 480 605 915 1089 981 174 15 565 Fragments arranged by size: 1089 981 915 605 565 480 174 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 377 409 4824 Size: 377 32 4415 Fragments arranged by size: 4415 377 32 Tth111I GACn'n_nGTC Cuts at: 0 120 1559 4824 Size: 120 1439 3265 Fragments arranged by size: 3265 1439 120 XbaI T'CTAG_A Cuts at: 0 201 4824 Size: 201 4623 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1727 3018 4483 4763 4824 Size: 1727 1291 1465 280 61 Fragments arranged by size: 1727 1465 1291 280 61 XmnI GAAnn'nnTTC Cuts at: 0 2248 3149 4177 4824 Size: 2248 901 1028 647 Fragments arranged by size: 2248 1028 901 647 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AhdI AlfI AloI AlwNI ApoI AseI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BclI BfrBI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PciI PfoI PpiI PpuMI PsrI PstI PvuI PvuII SacI SalI SapI ScaI SfcI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AleI ApaI ApaLI AscI AsiSI BbeI BciVI BglI BglII BmgBI BmtI BplI BsaHI BsaXI BsiWI BsmBI BspEI BsrBI BssHII BstAPI BstZ17I CspCI DraIII DrdI EciI EcoRV FseI FspAI HaeII HpaI KasI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI RsrII SacII SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI XhoI XmaI ZraI