(Circular) (Six-base) MAPSORT of: pFN21AE2688.seq Check: 6097 from: 1 to: 8887 pFN21AE2688 8887 bp With 182 enzymes: SgfI * May 11, 2017 14:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3352 6697 278 Size: 53 83 186 936 1816 3345 2468 Fragments arranged by size: 3345 2468 1816 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4171 6230 1937 Size: 2234 2059 4594 Fragments arranged by size: 4594 2234 2059 Acc65I G'GTAC_C Cuts at: 6208 6208 Size: 8887 AclI AA'CG_TT Cuts at: 1563 7014 7387 1563 Size: 5451 373 3063 Fragments arranged by size: 5451 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2447 2923 3631 4337 4757 5604 6113 6950 Size: 815 476 708 706 420 847 509 837 Cuts at: 6950 8334 8602 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 847 837 815 708 706 509 476 420 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8690 AflIII A'CryG_T Cuts at: 1203 2990 7786 8763 1203 Size: 1787 4796 977 1327 Fragments arranged by size: 4796 1787 1327 977 AgeI A'CCGG_T Cuts at: 7718 7718 Size: 8887 AhdI GACnn_n'nnGTC Cuts at: 7616 7616 Size: 8887 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8887 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8887 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5091 5123 5091 Size: 32 8855 AlwNI CAG_nnn'CTG Cuts at: 2872 5102 6630 8202 2872 Size: 2230 1528 1572 3557 Fragments arranged by size: 3557 2230 1572 1528 ApaI G_GGCC'C Cuts at: 2145 2145 Size: 8887 ApaLI G'TGCA_C Cuts at: 6944 8100 6944 Size: 1156 7731 ApoI r'AATT_y Cuts at: 1493 2435 2537 2973 3072 3855 3952 4159 4211 Size: 942 102 436 99 783 97 207 52 Cuts at: 4211 5074 5884 6143 6196 6422 6574 8683 1493 Size: 863 810 259 53 226 152 2109 1697 Fragments arranged by size: 2109 1697 942 863 810 783 436 259 226 207 152 102 99 97 53 52 AseI AT'TA_AT Cuts at: 160 1032 7441 160 Size: 872 6409 1606 Fragments arranged by size: 6409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8887 AvaI C'yCGr_G Cuts at: 1870 1943 2095 3726 6212 1870 Size: 73 152 1631 2486 4545 Fragments arranged by size: 4545 2486 1631 152 73 AvrII C'CTAG_G Cuts at: 2356 2356 Size: 8887 BamHI G'GATC_C Cuts at: 6217 6584 6217 Size: 367 8520 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2648 6208 7663 618 Size: 325 217 627 861 3560 1455 1842 Fragments arranged by size: 3560 1842 1455 861 627 325 217 BanII G_rGCy'C Cuts at: 1391 2145 2168 2263 4759 5821 6206 1391 Size: 754 23 95 2496 1062 385 4072 Fragments arranged by size: 4072 2496 1062 754 385 95 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5517 928 Size: 971 3618 4298 Fragments arranged by size: 4298 3618 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7078 7112 1903 Size: 34 5141 34 3678 Fragments arranged by size: 5141 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2964 6779 7989 2964 Size: 3815 1210 3862 Fragments arranged by size: 3862 3815 1210 BclI T'GATC_A Cuts at: 1548 3421 1548 Size: 1873 7014 BfrBI ATG'CAT Cuts at: 2831 2831 Size: 8887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2051 7498 136 Size: 107 122 71 631 605 379 5447 1525 Fragments arranged by size: 5447 1525 631 605 379 122 107 71 BglII A'GATC_T Cuts at: 2275 8882 2275 Size: 6607 2280 BlpI GC'TnA_GC Cuts at: 6304 6304 Size: 8887 Bme1580I G_kGCm'C Cuts at: 1790 2145 2716 6948 8104 1790 Size: 355 571 4232 1156 2573 Fragments arranged by size: 4232 2573 1156 571 355 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8887 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2684 6618 7576 Size: 416 53 83 399 24 1267 3934 958 Cuts at: 7576 442 Size: 1753 Fragments arranged by size: 3934 1753 1267 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8887 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2894 3857 4728 6123 7547 1239 Size: 1655 963 871 1395 1424 2579 Fragments arranged by size: 2579 1655 1424 1395 963 871 Bpu10I CC'TnA_GC Cuts at: 2347 2347 Size: 8887 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3513 4550 7012 7877 8175 3513 Size: 1037 2462 865 298 4225 Fragments arranged by size: 4225 2462 1037 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4860 5279 7550 882 Size: 617 3361 419 2271 2219 Fragments arranged by size: 3361 2271 2219 617 419 BsaAI yAC'GTr Cuts at: 493 1204 2387 6678 493 Size: 711 1183 4291 2702 Fragments arranged by size: 4291 2702 1183 711 BsaBI GATnn'nnATC Cuts at: 5717 8881 5717 Size: 3164 5723 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3349 6694 7076 7711 Size: 53 83 186 936 1816 3345 382 635 Cuts at: 7711 275 Size: 1451 Fragments arranged by size: 3345 1816 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7320 7718 7992 8139 8550 7320 Size: 398 274 147 411 7657 Fragments arranged by size: 7657 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 6096 6126 1178 Size: 30 165 30 4693 30 3939 Fragments arranged by size: 4693 3939 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2228 3821 5699 1186 Size: 886 156 1593 1878 4374 Fragments arranged by size: 4374 1878 1593 886 156 BseYI C'CCAG_C Cuts at: 2167 2948 8090 8586 2167 Size: 781 5142 496 2468 Fragments arranged by size: 5142 2468 781 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 8314 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6337 7098 8126 1540 Size: 275 186 4336 761 1028 2301 Fragments arranged by size: 4336 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4759 5821 6948 7033 8104 4759 Size: 1062 1127 85 1071 5542 Fragments arranged by size: 5542 1127 1071 1062 85 BsmI GAATG_Cn' Cuts at: 2301 2758 2833 3402 3879 5179 5276 6398 6491 Size: 457 75 569 477 1300 97 1122 93 Cuts at: 6491 2301 Size: 4697 Fragments arranged by size: 4697 1300 1122 569 477 457 97 93 75 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2168 2263 2716 4759 5821 6206 Size: 399 355 23 95 453 2043 1062 385 Cuts at: 6206 6948 7033 8104 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 2043 1071 1062 742 453 399 385 355 95 85 23 BspEI T'CCGG_A Cuts at: 8550 8550 Size: 8887 BspHI T'CATG_A Cuts at: 4579 5976 6776 8506 4579 Size: 1397 800 1730 4960 Fragments arranged by size: 4960 1730 1397 800 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2348 6242 844 Size: 1504 3894 3489 Fragments arranged by size: 3894 3489 1504 BsrBI CCG'CTC Cuts at: 1394 1823 2068 2132 2346 6774 1394 Size: 429 245 64 214 4428 3507 Fragments arranged by size: 4428 3507 429 245 214 64 BsrDI GCAATG_nn' Cuts at: 65 1248 2184 3024 3335 3599 4641 5268 5550 Size: 1183 936 840 311 264 1042 627 282 Cuts at: 5550 7382 7556 65 Size: 1832 174 1396 Fragments arranged by size: 1832 1396 1183 1042 936 840 627 311 282 264 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2100 4247 7531 7718 1700 Size: 110 290 2147 3284 187 2869 Fragments arranged by size: 3284 2869 2147 290 187 110 BsrGI T'GTAC_A Cuts at: 96 3696 4495 96 Size: 3600 799 4488 Fragments arranged by size: 4488 3600 799 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8887 BssSI C'ACGA_G Cuts at: 3477 4968 6947 7959 3477 Size: 1491 1979 1012 4405 Fragments arranged by size: 4405 1979 1491 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8887 BstBI TT'CG_AA Cuts at: 2287 7691 2287 Size: 5404 3483 BstEII G'GTnAC_C Cuts at: 1184 5012 1184 Size: 3828 5059 BstYI r'GATC_y Cuts at: 1972 2021 2275 2683 6217 6584 6969 6986 8438 Size: 49 254 408 3534 367 385 17 1452 Cuts at: 8438 8524 8536 8882 1972 Size: 86 12 346 1977 Fragments arranged by size: 3534 1977 1452 408 385 367 346 254 86 49 17 12 Bsu36I CC'TnA_GG Cuts at: 5879 5879 Size: 8887 BtgI C'CryG_G Cuts at: 513 1065 2004 2145 513 Size: 552 939 141 7255 Fragments arranged by size: 7255 939 552 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8148 Fragments arranged by size: 8148 683 56 BtsI GCAGTG_nn' Cuts at: 5433 6403 7197 7217 5433 Size: 970 794 20 7103 Fragments arranged by size: 7103 970 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8852 DraI TTT'AAA Cuts at: 6191 6191 Size: 8887 DraIII CAC_nnn'GTG Cuts at: 3145 3145 Size: 8887 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7894 809 Size: 507 6578 1802 Fragments arranged by size: 6578 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2296 3332 Size: 54 1425 50 275 18 318 148 1036 Cuts at: 3332 5451 5736 6334 7223 8852 8 Size: 2119 285 598 889 1629 43 Fragments arranged by size: 2119 1629 1425 1036 889 598 318 285 275 148 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6334 1537 Size: 275 4522 4090 Fragments arranged by size: 4522 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2418 4074 4691 6187 6817 1353 Size: 1065 1656 617 1496 630 3423 Fragments arranged by size: 3423 1656 1496 1065 630 617 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5741 7474 7848 7994 387 Size: 1064 138 4152 1733 374 146 1280 Fragments arranged by size: 4152 1733 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4757 5819 4757 Size: 1062 7825 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2447 2894 2923 3631 3857 4337 4728 Size: 393 815 447 29 708 226 480 391 Cuts at: 4728 4757 5604 6113 6123 6950 7547 8334 8602 Size: 29 847 509 10 827 597 787 268 Cuts at: 8602 1239 Size: 1524 Fragments arranged by size: 1524 847 827 815 787 708 597 509 480 447 393 391 268 226 29 29 10 EcoNI CCTnn'n_nnAGG Cuts at: 5006 5006 Size: 8887 EcoRI G'AATT_C Cuts at: 5074 5884 6196 6574 5074 Size: 810 312 378 7387 Fragments arranged by size: 7387 810 378 312 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2537 2537 Size: 8887 FspI TGC'GCA Cuts at: 7393 7393 Size: 8887 HaeII r_GCGC'y Cuts at: 1678 8034 1678 Size: 6356 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2230 2260 2262 2292 4741 Size: 32 135 32 658 30 2 30 2449 Cuts at: 4741 4773 5993 6025 6096 6128 7528 7560 7602 Size: 32 1220 32 71 32 1400 32 42 Cuts at: 7602 7634 1373 Size: 32 2626 Fragments arranged by size: 2626 2449 1400 1220 658 135 71 42 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 4172 6231 6477 669 Size: 988 281 2234 2059 246 3079 Fragments arranged by size: 3079 2234 2059 988 281 246 HindIII A'AGCT_T Cuts at: 748 2312 2417 6136 748 Size: 1564 105 3719 3499 Fragments arranged by size: 3719 3499 1564 105 HpaI GTT'AAC Cuts at: 6477 6477 Size: 8887 KpnI G_GTAC'C Cuts at: 6212 6212 Size: 8887 MfeI C'AATT_G Cuts at: 5841 6486 5841 Size: 645 8242 MluI A'CGCG_T Cuts at: 8763 8763 Size: 8887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3325 3332 4149 5673 8001 8185 1305 Size: 2020 7 817 1524 2328 184 2007 Fragments arranged by size: 2328 2020 2007 1524 817 184 7 MscI TGG'CCA Cuts at: 10 64 1489 1832 3334 5453 5738 10 Size: 54 1425 343 1502 2119 285 3159 Fragments arranged by size: 3159 2119 1502 1425 343 285 54 MslI CAynn'nnrTG Cuts at: 518 1166 2499 2774 4064 6845 7204 7363 518 Size: 648 1333 275 1290 2781 359 159 2042 Fragments arranged by size: 2781 2042 1333 1290 648 359 275 159 MspA1I CmG'CkG Cuts at: 1606 3835 6303 6980 8128 8373 1606 Size: 2229 2468 677 1148 245 2120 Fragments arranged by size: 2468 2229 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2102 2102 Size: 8887 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7396 Fragments arranged by size: 7396 939 552 NdeI CA'TA_TG Cuts at: 387 2833 5158 387 Size: 2446 2325 4116 Fragments arranged by size: 4116 2446 2325 NgoMIV G'CCGG_C Cuts at: 2100 2100 Size: 8887 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8887 NotI GC'GGCC_GC Cuts at: 6334 6334 Size: 8887 NsiI A_TGCA'T Cuts at: 2833 2833 Size: 8887 NspI r_CATG'y Cuts at: 1132 1229 2111 2732 2994 3087 4381 6245 7790 Size: 97 882 621 262 93 1294 1864 1545 Cuts at: 7790 1132 Size: 2229 Fragments arranged by size: 2229 1864 1545 1294 882 621 262 97 93 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8887 PciI A'CATG_T Cuts at: 2990 7786 2990 Size: 4796 4091 PflMI CCAn_nnn'nTGG Cuts at: 1108 3835 5449 1108 Size: 2727 1614 4546 Fragments arranged by size: 4546 2727 1614 PfoI T'CCnGG_A Cuts at: 3891 3891 Size: 8887 PmeI GTTT'AAAC Cuts at: 6191 6191 Size: 8887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6956 6988 8469 8501 6956 Size: 32 1481 32 7342 Fragments arranged by size: 7342 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 5435 1613 Size: 3822 5065 PsiI TTA'TAA Cuts at: 3762 5757 6457 3762 Size: 1995 700 6192 Fragments arranged by size: 6192 1995 700 PspOMI G'GGCC_C Cuts at: 2141 2141 Size: 8887 PspXI vC'TCGA_Gb Cuts at: 3726 3726 Size: 8887 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2380 2412 2380 Size: 32 8855 PstI C_TGCA'G Cuts at: 830 3228 3618 6239 830 Size: 2398 390 2621 3478 Fragments arranged by size: 3478 2621 2398 390 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8887 PvuII CAG'CTG Cuts at: 3835 3835 Size: 8887 SacI G_AGCT'C Cuts at: 4759 5821 4759 Size: 1062 7825 SalI G'TCGA_C Cuts at: 1936 4170 6229 1936 Size: 2234 2059 4594 Fragments arranged by size: 4594 2234 2059 SbfI CC_TGCA'GG Cuts at: 6239 6239 Size: 8887 ScaI AGT'ACT Cuts at: 7135 8847 7135 Size: 1712 7175 SfcI C'TryA_G Cuts at: 826 1045 3224 3614 3871 5212 5825 6235 7370 Size: 219 2179 390 257 1341 613 410 1135 Cuts at: 7370 8051 8242 826 Size: 681 191 1471 Fragments arranged by size: 2179 1471 1341 1135 681 613 410 390 257 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8887 SmaI CCC'GGG Cuts at: 1872 2097 6214 1872 Size: 225 4117 4545 Fragments arranged by size: 4545 4117 225 SmlI C'TyrA_G Cuts at: 820 1017 1943 3528 3726 4529 6991 7892 8154 Size: 197 926 1585 198 803 2462 901 262 Cuts at: 8154 820 Size: 1553 Fragments arranged by size: 2462 1585 1553 926 901 803 262 198 197 SnaBI TAC'GTA Cuts at: 493 2387 6678 493 Size: 1894 4291 2702 Fragments arranged by size: 4291 2702 1894 SpeI A'CTAG_T Cuts at: 152 152 Size: 8887 SphI G_CATG'C Cuts at: 1132 2111 2732 3087 6245 1132 Size: 979 621 355 3158 3774 Fragments arranged by size: 3774 3158 979 621 355 SrfI GCCC'GGGC Cuts at: 2097 2097 Size: 8887 SspI AAT'ATT Cuts at: 5 52 6811 5 Size: 47 6759 2081 Fragments arranged by size: 6759 2081 47 StuI AGG'CCT Cuts at: 1517 4263 1517 Size: 2746 6141 StyI C'CwwG_G Cuts at: 513 1065 2004 2356 3801 4571 6326 513 Size: 552 939 352 1445 770 1755 3074 Fragments arranged by size: 3074 1755 1445 939 770 552 352 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3748 6914 6931 7084 7269 7714 325 Size: 1255 2168 3166 17 153 185 445 1498 Fragments arranged by size: 3166 2168 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3412 3696 4495 Size: 274 80 33 51 1443 1435 284 799 Cuts at: 4495 4875 7133 8845 96 Size: 380 2258 1712 138 Fragments arranged by size: 2258 1712 1443 1435 799 380 284 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8847 8879 8847 Size: 32 8855 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8887 XbaI T'CTAG_A Cuts at: 6223 6223 Size: 8887 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4382 4382 Size: 8887 XhoI C'TCGA_G Cuts at: 1943 3726 1943 Size: 1783 7104 XmaI C'CCGG_G Cuts at: 1870 2095 6212 1870 Size: 225 4117 4545 Fragments arranged by size: 4545 4117 225 XmnI GAAnn'nnTTC Cuts at: 2763 3306 5276 5484 5934 7016 2763 Size: 543 1970 208 450 1082 4634 Fragments arranged by size: 4634 1970 1082 543 450 208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3350 6695 276 Size: 53 83 186 936 1816 3345 2468 Fragments arranged by size: 3345 2468 1816 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I EcoO109I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PpuMI RsrII SacII SanDI SapI SexAI SfiI SfoI SwaI