(Linear) (Six-base) MAPSORT of: KIEE2688.seq Check: 1437 from: 1 to: 4182 KIEE2688.orf With 182 enzymes: SgfI * May 11, 2017 11:17 .. AatII G_ACGT'C Cuts at: 0 1347 4182 Size: 1347 2835 AccI GT'mk_AC Cuts at: 0 2166 4182 Size: 2166 2016 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 442 918 1626 2332 2752 3599 4108 4182 Size: 442 476 708 706 420 847 509 74 Fragments arranged by size: 847 708 706 509 476 442 420 74 AflIII A'CryG_T Cuts at: 0 985 4182 Size: 985 3197 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3086 3118 4182 Size: 3086 32 1064 Fragments arranged by size: 3086 1064 32 AlwNI CAG_nnn'CTG Cuts at: 0 867 3097 4182 Size: 867 2230 1085 Fragments arranged by size: 2230 1085 867 ApaI G_GGCC'C Cuts at: 0 140 4182 Size: 140 4042 ApoI r'AATT_y Cuts at: 0 430 532 968 1067 1850 1947 2154 2206 Size: 430 102 436 99 783 97 207 52 Cuts at: 2206 3069 3879 4138 4182 Size: 863 810 259 44 Fragments arranged by size: 863 810 783 436 430 259 207 102 99 97 52 44 AvaI C'yCGr_G Cuts at: 0 90 1721 4182 Size: 90 1631 2461 Fragments arranged by size: 2461 1631 90 AvrII C'CTAG_G Cuts at: 0 351 4182 Size: 351 3831 BanI G'GyrC_C Cuts at: 0 643 4182 Size: 643 3539 BanII G_rGCy'C Cuts at: 0 140 163 258 2754 3816 4182 Size: 140 23 95 2496 1062 366 Fragments arranged by size: 2496 1062 366 140 95 23 BbsI GAAGACnn'nnnn_ Cuts at: 0 3512 4182 Size: 3512 670 BciVI GTATCCnnnnn_n' Cuts at: 0 959 4182 Size: 959 3223 BclI T'GATC_A Cuts at: 0 1416 4182 Size: 1416 2766 BfrBI ATG'CAT Cuts at: 0 826 4182 Size: 826 3356 BglI GCCn_nnn'nGGC Cuts at: 0 46 4182 Size: 46 4136 BglII A'GATC_T Cuts at: 0 270 4182 Size: 270 3912 Bme1580I G_kGCm'C Cuts at: 0 140 711 4182 Size: 140 571 3471 Fragments arranged by size: 3471 571 140 BmrI ACTGGGnnnn_n' Cuts at: 0 679 4182 Size: 679 3503 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 889 1852 2723 4118 4182 Size: 889 963 871 1395 64 Fragments arranged by size: 1395 963 889 871 64 Bpu10I CC'TnA_GC Cuts at: 0 342 4182 Size: 342 3840 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1508 2545 4182 Size: 1508 1037 1637 Fragments arranged by size: 1637 1508 1037 BsaI GGTCTCn'nnnn_ Cuts at: 0 2855 3274 4182 Size: 2855 419 908 Fragments arranged by size: 2855 908 419 BsaAI yAC'GTr Cuts at: 0 382 4182 Size: 382 3800 BsaBI GATnn'nnATC Cuts at: 0 3712 4182 Size: 3712 470 BsaHI Gr'CG_yC Cuts at: 0 1344 4182 Size: 1344 2838 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 4091 4121 4182 Size: 4091 30 61 Fragments arranged by size: 4091 61 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 67 223 1816 3694 4182 Size: 67 156 1593 1878 488 Fragments arranged by size: 1878 1593 488 156 67 BseYI C'CCAG_C Cuts at: 0 162 943 4182 Size: 162 781 3239 Fragments arranged by size: 3239 781 162 BsiHKAI G_wGCw'C Cuts at: 0 2754 3816 4182 Size: 2754 1062 366 Fragments arranged by size: 2754 1062 366 BsmI GAATG_Cn' Cuts at: 0 296 753 828 1397 1874 3174 3271 4182 Size: 296 457 75 569 477 1300 97 911 Fragments arranged by size: 1300 911 569 477 457 296 97 75 Bsp1286I G_dGCh'C Cuts at: 0 140 163 258 711 2754 3816 4182 Size: 140 23 95 453 2043 1062 366 Fragments arranged by size: 2043 1062 453 366 140 95 23 BspHI T'CATG_A Cuts at: 0 2574 3971 4182 Size: 2574 1397 211 Fragments arranged by size: 2574 1397 211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 343 4182 Size: 343 3839 BsrBI CCG'CTC Cuts at: 0 63 127 341 4182 Size: 63 64 214 3841 Fragments arranged by size: 3841 214 64 63 BsrDI GCAATG_nn' Cuts at: 0 179 1019 1330 1594 2636 3263 3545 4182 Size: 179 840 311 264 1042 627 282 637 Fragments arranged by size: 1042 840 637 627 311 282 264 179 BsrFI r'CCGG_y Cuts at: 0 95 2242 4182 Size: 95 2147 1940 Fragments arranged by size: 2147 1940 95 BsrGI T'GTAC_A Cuts at: 0 1691 2490 4182 Size: 1691 799 1692 Fragments arranged by size: 1692 1691 799 BssSI C'ACGA_G Cuts at: 0 1472 2963 4182 Size: 1472 1491 1219 Fragments arranged by size: 1491 1472 1219 BstBI TT'CG_AA Cuts at: 0 282 4182 Size: 282 3900 BstEII G'GTnAC_C Cuts at: 0 3007 4182 Size: 3007 1175 BstYI r'GATC_y Cuts at: 0 16 270 678 4182 Size: 16 254 408 3504 Fragments arranged by size: 3504 408 254 16 Bsu36I CC'TnA_GG Cuts at: 0 3874 4182 Size: 3874 308 BtgI C'CryG_G Cuts at: 0 140 4182 Size: 140 4042 BtsI GCAGTG_nn' Cuts at: 0 3428 4182 Size: 3428 754 DraIII CAC_nnn'GTG Cuts at: 0 1140 4182 Size: 1140 3042 EaeI y'GGCC_r Cuts at: 0 143 291 1327 3446 3731 4182 Size: 143 148 1036 2119 285 451 Fragments arranged by size: 2119 1036 451 285 148 143 EarI CTCTTCn'nnn_ Cuts at: 0 413 2069 2686 4182 Size: 413 1656 617 1496 Fragments arranged by size: 1656 1496 617 413 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 3736 4182 Size: 3736 446 EcoICRI GAG'CTC Cuts at: 0 2752 3814 4182 Size: 2752 1062 368 Fragments arranged by size: 2752 1062 368 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 442 889 918 1626 1852 2332 2723 2752 Size: 442 447 29 708 226 480 391 29 Cuts at: 2752 3599 4108 4118 4182 Size: 847 509 10 64 Fragments arranged by size: 847 708 509 480 447 442 391 226 64 29 29 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 3001 4182 Size: 3001 1181 EcoRI G'AATT_C Cuts at: 0 3069 3879 4182 Size: 3069 810 303 Fragments arranged by size: 3069 810 303 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 532 4182 Size: 532 3650 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 225 255 257 287 2736 2768 3988 4020 Size: 225 30 2 30 2449 32 1220 32 Cuts at: 4020 4091 4123 4182 Size: 71 32 59 Fragments arranged by size: 2449 1220 225 71 59 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 0 2167 4182 Size: 2167 2015 HindIII A'AGCT_T Cuts at: 0 307 412 4131 4182 Size: 307 105 3719 51 Fragments arranged by size: 3719 307 105 51 MfeI C'AATT_G Cuts at: 0 3836 4182 Size: 3836 346 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1320 1327 2144 3668 4182 Size: 1320 7 817 1524 514 Fragments arranged by size: 1524 1320 817 514 7 MscI TGG'CCA Cuts at: 0 1329 3448 3733 4182 Size: 1329 2119 285 449 Fragments arranged by size: 2119 1329 449 285 MslI CAynn'nnrTG Cuts at: 0 494 769 2059 4182 Size: 494 275 1290 2123 Fragments arranged by size: 2123 1290 494 275 MspA1I CmG'CkG Cuts at: 0 1830 4182 Size: 1830 2352 NaeI GCC'GGC Cuts at: 0 97 4182 Size: 97 4085 NdeI CA'TA_TG Cuts at: 0 828 3153 4182 Size: 828 2325 1029 Fragments arranged by size: 2325 1029 828 NgoMIV G'CCGG_C Cuts at: 0 95 4182 Size: 95 4087 NsiI A_TGCA'T Cuts at: 0 828 4182 Size: 828 3354 NspI r_CATG'y Cuts at: 0 106 727 989 1082 2376 4182 Size: 106 621 262 93 1294 1806 Fragments arranged by size: 1806 1294 621 262 106 93 PciI A'CATG_T Cuts at: 0 985 4182 Size: 985 3197 PflMI CCAn_nnn'nTGG Cuts at: 0 1830 3444 4182 Size: 1830 1614 738 Fragments arranged by size: 1830 1614 738 PfoI T'CCnGG_A Cuts at: 0 1886 4182 Size: 1886 2296 PshAI GACnn'nnGTC Cuts at: 0 3430 4182 Size: 3430 752 PsiI TTA'TAA Cuts at: 0 1757 3752 4182 Size: 1757 1995 430 Fragments arranged by size: 1995 1757 430 PspOMI G'GGCC_C Cuts at: 0 136 4182 Size: 136 4046 PspXI vC'TCGA_Gb Cuts at: 0 1721 4182 Size: 1721 2461 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 375 407 4182 Size: 375 32 3775 Fragments arranged by size: 3775 375 32 PstI C_TGCA'G Cuts at: 0 1223 1613 4182 Size: 1223 390 2569 Fragments arranged by size: 2569 1223 390 PvuII CAG'CTG Cuts at: 0 1830 4182 Size: 1830 2352 SacI G_AGCT'C Cuts at: 0 2754 3816 4182 Size: 2754 1062 366 Fragments arranged by size: 2754 1062 366 SalI G'TCGA_C Cuts at: 0 2165 4182 Size: 2165 2017 SfcI C'TryA_G Cuts at: 0 1219 1609 1866 3207 3820 4182 Size: 1219 390 257 1341 613 362 Fragments arranged by size: 1341 1219 613 390 362 257 SmaI CCC'GGG Cuts at: 0 92 4182 Size: 92 4090 SmlI C'TyrA_G Cuts at: 0 1523 1721 2524 4182 Size: 1523 198 803 1658 Fragments arranged by size: 1658 1523 803 198 SnaBI TAC'GTA Cuts at: 0 382 4182 Size: 382 3800 SphI G_CATG'C Cuts at: 0 106 727 1082 4182 Size: 106 621 355 3100 Fragments arranged by size: 3100 621 355 106 SrfI GCCC'GGGC Cuts at: 0 92 4182 Size: 92 4090 StuI AGG'CCT Cuts at: 0 2258 4182 Size: 2258 1924 StyI C'CwwG_G Cuts at: 0 351 1796 2566 4182 Size: 351 1445 770 1616 Fragments arranged by size: 1616 1445 770 351 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1743 4182 Size: 1743 2439 TatI w'GTAC_w Cuts at: 0 1407 1691 2490 2870 4182 Size: 1407 284 799 380 1312 Fragments arranged by size: 1407 1312 799 380 284 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2377 4182 Size: 2377 1805 XhoI C'TCGA_G Cuts at: 0 1721 4182 Size: 1721 2461 XmaI C'CCGG_G Cuts at: 0 90 4182 Size: 90 4092 XmnI GAAnn'nnTTC Cuts at: 0 758 1301 3271 3479 3929 4182 Size: 758 543 1970 208 450 253 Fragments arranged by size: 1970 758 543 450 253 208 ZraI GAC'GTC Cuts at: 0 1345 4182 Size: 1345 2837 Enzymes that do cut: AatII AccI AcuI AflIII AloI AlwNI ApaI ApoI AvaI AvrII BanI BanII BbsI BciVI BclI BfrBI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV NsiI NspI PciI PflMI PfoI PshAI PsiI PspOMI PspXI PsrI PstI PvuII SacI SalI SfcI SmaI SmlI SnaBI SphI SrfI StuI StyI TaqII TatI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BlpI BmgBI BmtI BplI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BssHII BstAPI BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoO109I EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MluI NarI NcoI NheI NotI NruI PacI PasI PmeI PmlI PpiI PpuMI PvuI RsrII SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SpeI SspI SwaI TstI Tth111I XbaI