################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccII AccIII AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AviII AvrII BbeI BbrPI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoT22I FseI FspI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AclI 376 AACGTT AA^CGTT AocI 3959 CCTCAGG CC^T[ACGT]AGG AseI 301 ATTAAT AT^TAAT AsnI 301 ATTAAT AT^TAAT BalI 3740 TGGCCA TGG^CCA BamHI 3455 GGATCC G^GATCC BanI 716 GGCACC G^G[CT][GA]CC BclI 3878 TGATCA T^GATCA BglI 3570 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 4019 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1697 ACCGGA [AT]^CCGG[AT] BsgI 1868 CTGCAC GTGCAG(16/14) BspMI 2215 GCAGGT ACCTGC(4/8) BsrDI 2528 GCAATG GCAATG(2/0) BssSI 1667 CTCGTG CACGAG(-5/-1) BstAPI 4001 GCAACGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 3959 CCTCAGG CC^T[ACGT]AGG DraIII 2344 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 2318 CCATGG C^C[GA][CT]GG Eco81I 3959 CCTCAGG CC^T[ACGT]AGG EcoNI 2727 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 790 GATATC GAT^ATC FauI 3150 GCGGG CCCGC(4/6) FbaI 3878 TGATCA T^GATCA HpaI 1719 GTTAAC GTT^AAC MamI 4019 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 3740 TGGCCA TGG^CCA MscI 3740 TGGCCA TGG^CCA NcoI 2318 CCATGG C^CATGG NdeI 3834 CATATG CA^TATG PaeR7I 762 CTCGAG C^TCGAG PflMI 3554 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 3622 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 301 ATTAAT AT^TAAT Psp1406I 376 AACGTT AA^CGTT ScaI 2816 AGTACT AGT^ACT StuI 3184 AGGCCT AGG^CCT Van91I 3554 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 270 TCTAGA T^CTAGA XcmI 3728 CCACCACAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 762 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 319 GGCGTC G[GA]^CG[CT]C 2139 GACGCC AlwNI 156 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG 3962 CAGGAGCTG AspI 154 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1455 GACTGTGTC BcnI 1035 CCCGG CC^[GC]GG 1878 CCCGG BsaHI 319 GGCGTC G[GA]^CG[CT]C 2139 GACGCC BsrBI 2686 GAGCGG CCGCTC(-3/-3) 3148 GAGCGG EaeI 3740 TGGCCA [CT]^GGCC[GA] 4033 CGGCCA EcoRI 2513 GAATTC G^AATTC 2808 GAATTC HaeII 2363 GGCGCT [GA]GCGC^[CT] 3131 AGCGCC Hin1I 319 GGCGTC G[GA]^CG[CT]C 2139 GACGCC HincII 1719 GTTAAC GT[CT]^[GA]AC 3478 GTCAAC HindII 1719 GTTAAC GT[CT]^[GA]AC 3478 GTCAAC NciI 1035 CCCGG CC^[GC]GG 1878 CCCGG NspI 2855 GCATGT [GA]CATG^[CT] 3671 ACATGC PflFI 154 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1455 GACTGTGTC SacI 76 GAGCTC GAGCT^C 83 GAGCTC SapI 3064 GAAGAGC GCTCTTC(1/4) 3953 GCTCTTC SspI 2488 AATATT AAT^ATT 3314 AATATT Tth111I 154 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1455 GACTGTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaI AvaII BanII BbsI BbvCI BbvI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI