(Circular) (Six-base) MAPSORT of: pFN21AE2704.seq Check: 1588 from: 1 to: 8752 pFN21AE2704 8752 bp With 182 enzymes: SgfI * September 21, 2017 12:07 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6562 278 Size: 53 83 186 936 5026 2468 Fragments arranged by size: 5026 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2351 2959 3386 6095 1937 Size: 414 608 427 2709 4594 Fragments arranged by size: 4594 2709 608 427 414 Acc65I G'GTAC_C Cuts at: 6073 6073 Size: 8752 AclI AA'CG_TT Cuts at: 1563 2382 6879 7252 1563 Size: 819 4497 373 3063 Fragments arranged by size: 4497 3063 819 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3246 3504 4466 4914 5048 5075 5217 5399 Size: 1614 258 962 448 134 27 142 182 Cuts at: 5399 5694 5769 5994 6815 8199 8467 1632 Size: 295 75 225 821 1384 268 1917 Fragments arranged by size: 1917 1614 1384 962 821 448 295 268 258 225 182 142 134 75 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8555 AflIII A'CryG_T Cuts at: 1203 7651 8628 1203 Size: 6448 977 1327 Fragments arranged by size: 6448 1327 977 AgeI A'CCGG_T Cuts at: 7583 7583 Size: 8752 AhdI GACnn_n'nnGTC Cuts at: 7481 7481 Size: 8752 AleI CACnn'nnGTG Cuts at: 1166 5741 1166 Size: 4575 4177 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8752 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4827 4859 5675 5707 4827 Size: 32 816 32 7872 Fragments arranged by size: 7872 816 32 32 AlwNI CAG_nnn'CTG Cuts at: 2166 5972 6495 8067 2166 Size: 3806 523 1572 2851 Fragments arranged by size: 3806 2851 1572 523 ApaLI G'TGCA_C Cuts at: 6809 7965 6809 Size: 1156 7596 ApoI r'AATT_y Cuts at: 1493 2097 2203 2245 2702 2846 3525 3582 4235 Size: 604 106 42 457 144 679 57 653 Cuts at: 4235 4422 4518 4813 6061 6287 6439 8548 1493 Size: 187 96 295 1248 226 152 2109 1697 Fragments arranged by size: 2109 1697 1248 679 653 604 457 295 226 187 152 144 106 96 57 42 AseI AT'TA_AT Cuts at: 160 1032 2307 7306 160 Size: 872 1275 4999 1606 Fragments arranged by size: 4999 1606 1275 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8752 AvaI C'yCGr_G Cuts at: 1870 1943 2767 4660 4947 5767 6077 1870 Size: 73 824 1893 287 820 310 4545 Fragments arranged by size: 4545 1893 824 820 310 287 73 BamHI G'GATC_C Cuts at: 5460 6082 6449 5460 Size: 622 367 7763 Fragments arranged by size: 7763 622 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2721 6073 7528 618 Size: 325 217 627 934 3352 1455 1842 Fragments arranged by size: 3352 1842 1455 934 627 325 217 BanII G_rGCy'C Cuts at: 1391 2085 2092 2768 4884 6071 1391 Size: 694 7 676 2116 1187 4072 Fragments arranged by size: 4072 2116 1187 694 676 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2637 3627 3840 4020 4920 5495 5732 Size: 971 738 990 213 180 900 575 237 Cuts at: 5732 928 Size: 3948 Fragments arranged by size: 3948 990 971 900 738 575 237 213 180 BbvCI CC'TCA_GC Cuts at: 5097 5103 5646 5097 Size: 6 543 8203 Fragments arranged by size: 8203 543 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6943 6977 1903 Size: 34 5006 34 3678 Fragments arranged by size: 5006 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6644 7854 6644 Size: 1210 7542 BclI T'GATC_A Cuts at: 1548 5883 1548 Size: 4335 4417 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5581 7363 136 Size: 107 122 71 631 605 3909 1782 1525 Fragments arranged by size: 3909 1782 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2443 3265 3788 5267 8747 2443 Size: 822 523 1479 3480 2448 Fragments arranged by size: 3480 2448 1479 822 523 BlpI GC'TnA_GC Cuts at: 6169 6169 Size: 8752 Bme1580I G_kGCm'C Cuts at: 1790 4660 5703 6813 7969 1790 Size: 2870 1043 1110 1156 2573 Fragments arranged by size: 2870 2573 1156 1110 1043 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8752 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6483 7441 442 Size: 416 53 83 399 24 5066 958 1753 Fragments arranged by size: 5066 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8752 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5592 5592 Size: 8752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2460 2620 3452 4083 4930 5677 7412 1239 Size: 1221 160 832 631 847 747 1735 2579 Fragments arranged by size: 2579 1735 1221 847 832 747 631 160 Bpu10I CC'TnA_GC Cuts at: 4329 4847 5097 5103 5646 4329 Size: 518 250 6 543 7435 Fragments arranged by size: 7435 543 518 250 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3335 4081 4320 6877 7742 8040 3335 Size: 746 239 2557 865 298 4047 Fragments arranged by size: 4047 2557 865 746 298 239 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7415 882 Size: 617 5916 2219 Fragments arranged by size: 5916 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6543 493 Size: 711 5339 2702 Fragments arranged by size: 5339 2702 711 BsaBI GATnn'nnATC Cuts at: 6028 8746 6028 Size: 2718 6034 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2325 4145 6559 6941 Size: 53 83 186 936 792 1820 2414 382 Cuts at: 6941 7576 275 Size: 635 1451 Fragments arranged by size: 2414 1820 1451 936 792 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3702 7185 7583 7857 8004 8415 3702 Size: 3483 398 274 147 411 4039 Fragments arranged by size: 4039 3483 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5531 5561 5675 5705 5961 Size: 30 165 30 4128 30 114 30 256 Cuts at: 5961 5991 1178 Size: 30 3939 Fragments arranged by size: 4128 3939 256 165 114 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2703 2779 3182 4700 4703 4976 4981 5120 Size: 1517 76 403 1518 3 273 5 139 Cuts at: 5120 5141 5371 5680 1186 Size: 21 230 309 4258 Fragments arranged by size: 4258 1518 1517 403 309 273 230 139 76 21 5 3 BseYI C'CCAG_C Cuts at: 2424 2530 3622 4228 4668 7955 8451 2424 Size: 106 1092 606 440 3287 496 2725 Fragments arranged by size: 3287 2725 1092 606 496 440 106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3858 1163 Size: 573 2122 6057 Fragments arranged by size: 6057 2122 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6202 6963 7991 1540 Size: 275 186 4201 761 1028 2301 Fragments arranged by size: 4201 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2085 2092 2122 3656 6813 6898 7969 2085 Size: 7 30 1534 3157 85 1071 2868 Fragments arranged by size: 3157 2868 1534 1071 85 30 7 BsmI GAATG_Cn' Cuts at: 6263 6356 6263 Size: 93 8659 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2092 2122 2768 3656 4660 4884 Size: 399 295 7 30 646 888 1004 224 Cuts at: 4884 5703 6071 6813 6898 7969 1391 Size: 819 368 742 85 1071 2174 Fragments arranged by size: 2174 1071 1004 888 819 742 646 399 368 295 224 85 30 7 BspEI T'CCGG_A Cuts at: 8415 8415 Size: 8752 BspHI T'CATG_A Cuts at: 4522 4993 5514 6641 8371 4522 Size: 471 521 1127 1730 4903 Fragments arranged by size: 4903 1730 1127 521 471 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4211 6107 844 Size: 3367 1896 3489 Fragments arranged by size: 3489 3367 1896 BsrBI CCG'CTC Cuts at: 1394 1823 4693 5155 6639 1394 Size: 429 2870 462 1484 3507 Fragments arranged by size: 3507 2870 1484 462 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4540 7247 7421 65 Size: 1183 3292 2707 174 1396 Fragments arranged by size: 3292 2707 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7396 7583 1700 Size: 110 5586 187 2869 Fragments arranged by size: 5586 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8752 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8752 BssSI C'ACGA_G Cuts at: 3672 6812 7824 3672 Size: 3140 1012 4600 Fragments arranged by size: 4600 3140 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 6012 794 Size: 5218 3534 BstBI TT'CG_AA Cuts at: 7556 7556 Size: 8752 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8752 BstXI CCAn_nnnn'nTGG Cuts at: 4148 4825 5743 4148 Size: 677 918 7157 Fragments arranged by size: 7157 918 677 BstYI r'GATC_y Cuts at: 1972 2443 3265 3788 4100 4197 5267 5360 5460 Size: 471 822 523 312 97 1070 93 100 Cuts at: 5460 6082 6449 6834 6851 8303 8389 8401 8747 Size: 622 367 385 17 1452 86 12 346 Cuts at: 8747 1972 Size: 1977 Fragments arranged by size: 1977 1452 1070 822 622 523 471 385 367 346 312 100 97 93 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5965 5965 Size: 8752 BtgI C'CryG_G Cuts at: 513 1065 4323 6048 513 Size: 552 3258 1725 3217 Fragments arranged by size: 3258 3217 1725 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8013 Fragments arranged by size: 8013 683 56 BtsI GCAGTG_nn' Cuts at: 5479 6268 7062 7082 5479 Size: 789 794 20 7149 Fragments arranged by size: 7149 794 789 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3840 3875 529 Size: 35 3276 35 5406 Fragments arranged by size: 5406 3276 35 35 DraI TTT'AAA Cuts at: 6056 6056 Size: 8752 DraIII CAC_nnn'GTG Cuts at: 4354 4354 Size: 8752 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7759 809 Size: 507 6443 1802 Fragments arranged by size: 6443 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5745 6038 6199 Size: 54 1425 50 275 18 3915 293 161 Cuts at: 6199 7088 8717 8 Size: 889 1629 43 Fragments arranged by size: 3915 1629 1425 889 293 275 161 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6199 1537 Size: 275 4387 4090 Fragments arranged by size: 4387 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 2478 3279 3492 4148 4770 4953 5064 Size: 719 406 801 213 656 622 183 111 Cuts at: 5064 5353 5423 5515 5965 6682 1353 Size: 289 70 92 450 717 3423 Fragments arranged by size: 3423 801 719 717 656 622 450 406 289 213 183 111 92 70 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7339 7713 7859 387 Size: 1064 138 5750 374 146 1280 Fragments arranged by size: 5750 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2083 2090 2083 Size: 7 8745 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2460 2620 3246 3452 3504 4083 4466 Size: 393 828 160 626 206 52 579 383 Cuts at: 4466 4914 4930 5048 5075 5217 5399 5677 5694 Size: 448 16 118 27 142 182 278 17 Cuts at: 5694 5769 5994 6815 7412 8199 8467 1239 Size: 75 225 821 597 787 268 1524 Fragments arranged by size: 1524 828 821 787 626 597 579 448 393 383 278 268 225 206 182 160 142 118 75 52 27 17 16 EcoNI CCTnn'n_nnAGG Cuts at: 4736 4736 Size: 8752 EcoRI G'AATT_C Cuts at: 4518 4813 6061 6439 4518 Size: 295 1248 378 6831 Fragments arranged by size: 6831 1248 378 295 EcoRV GAT'ATC Cuts at: 2797 2797 Size: 8752 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2444 3276 4554 2444 Size: 832 1278 6642 Fragments arranged by size: 6642 1278 832 FspI TGC'GCA Cuts at: 7258 7258 Size: 8752 HaeII r_GCGC'y Cuts at: 1678 4372 5140 7899 1678 Size: 2694 768 2759 2531 Fragments arranged by size: 2759 2694 2531 768 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2104 2136 2457 2489 2870 Size: 32 135 32 532 32 321 32 381 Cuts at: 2870 2902 3353 3385 4037 4069 5276 5308 5961 Size: 32 451 32 652 32 1207 32 653 Cuts at: 5961 5993 7393 7425 7467 7499 1373 Size: 32 1400 32 42 32 2626 Fragments arranged by size: 2626 1400 1207 653 652 532 451 381 321 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3726 5485 6096 6342 669 Size: 988 281 1788 1759 611 246 3079 Fragments arranged by size: 3079 1788 1759 988 611 281 246 HindIII A'AGCT_T Cuts at: 748 2312 2394 2749 3907 748 Size: 1564 82 355 1158 5593 Fragments arranged by size: 5593 1564 1158 355 82 HpaI GTT'AAC Cuts at: 3726 6342 3726 Size: 2616 6136 KpnI G_GTAC'C Cuts at: 6077 6077 Size: 8752 MfeI C'AATT_G Cuts at: 6351 6351 Size: 8752 MluI A'CGCG_T Cuts at: 8628 8628 Size: 8752 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2027 4670 5488 7866 8050 1305 Size: 722 2643 818 2378 184 2007 Fragments arranged by size: 2643 2378 2007 818 722 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5747 10 Size: 54 1425 343 3915 3015 Fragments arranged by size: 3915 3015 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2135 3659 5741 6710 7069 7228 518 Size: 648 969 1524 2082 969 359 159 2042 Fragments arranged by size: 2082 2042 1524 969 969 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3157 5457 5650 5714 5809 6168 6845 7993 Size: 1551 2300 193 64 95 359 677 1148 Cuts at: 7993 8238 1606 Size: 245 2120 Fragments arranged by size: 2300 2120 1551 1148 677 359 245 193 95 64 NcoI C'CATG_G Cuts at: 513 1065 4323 6048 513 Size: 552 3258 1725 3217 Fragments arranged by size: 3258 3217 1725 552 NdeI CA'TA_TG Cuts at: 387 5840 387 Size: 5453 3299 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8752 NotI GC'GGCC_GC Cuts at: 6199 6199 Size: 8752 NspI r_CATG'y Cuts at: 1132 1229 4864 5680 6110 7655 1132 Size: 97 3635 816 430 1545 2229 Fragments arranged by size: 3635 2229 1545 816 430 97 PasI CC'CwG_GG Cuts at: 1348 5896 1348 Size: 4548 4204 PciI A'CATG_T Cuts at: 7651 7651 Size: 8752 PflMI CCAn_nnn'nTGG Cuts at: 1108 5565 1108 Size: 4457 4295 PfoI T'CCnGG_A Cuts at: 2437 2437 Size: 8752 PmeI GTTT'AAAC Cuts at: 6056 6056 Size: 8752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5330 5362 5675 5707 6821 6853 8334 8366 5330 Size: 32 313 32 1114 32 1481 32 5716 Fragments arranged by size: 5716 1481 1114 313 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 5631 1613 Size: 4018 4734 PsiI TTA'TAA Cuts at: 2871 6322 2871 Size: 3451 5301 PspXI vC'TCGA_Gb Cuts at: 2767 2767 Size: 8752 PstI C_TGCA'G Cuts at: 830 2171 3273 3360 4740 6104 830 Size: 1341 1102 87 1380 1364 3478 Fragments arranged by size: 3478 1380 1364 1341 1102 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8752 PvuII CAG'CTG Cuts at: 3157 5457 5650 5714 5809 3157 Size: 2300 193 64 95 6100 Fragments arranged by size: 6100 2300 193 95 64 SacI G_AGCT'C Cuts at: 2085 2092 2085 Size: 7 8745 SalI G'TCGA_C Cuts at: 1936 6094 1936 Size: 4158 4594 SapI GCTCTTCn'nnn_ Cuts at: 5064 5965 5064 Size: 901 7851 SbfI CC_TGCA'GG Cuts at: 6104 6104 Size: 8752 ScaI AGT'ACT Cuts at: 4823 7000 8712 4823 Size: 2177 1712 4863 Fragments arranged by size: 4863 2177 1712 SfcI C'TryA_G Cuts at: 826 1045 2167 2447 3269 3356 3387 4736 6100 Size: 219 1122 280 822 87 31 1349 1364 Cuts at: 6100 7235 7916 8107 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1364 1349 1135 1122 822 681 280 219 191 87 31 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8752 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8752 SmaI CCC'GGG Cuts at: 1872 6079 1872 Size: 4207 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2767 3314 4096 4335 6856 7757 Size: 197 926 824 547 782 239 2521 901 Cuts at: 7757 8019 820 Size: 262 1553 Fragments arranged by size: 2521 1553 926 901 824 782 547 262 239 197 SnaBI TAC'GTA Cuts at: 493 6543 493 Size: 6050 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8752 SphI G_CATG'C Cuts at: 1132 6110 1132 Size: 4978 3774 SspI AAT'ATT Cuts at: 5 52 4495 5321 6676 5 Size: 47 4443 826 1355 2081 Fragments arranged by size: 4443 2081 1355 826 47 StuI AGG'CCT Cuts at: 1517 5191 1517 Size: 3674 5078 StyI C'CwwG_G Cuts at: 513 1065 2717 4323 5002 5579 6048 6191 513 Size: 552 1652 1606 679 577 469 143 3074 Fragments arranged by size: 3074 1652 1606 679 577 552 469 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4660 6779 6796 6949 7134 7579 325 Size: 1255 3080 2119 17 153 185 445 1498 Fragments arranged by size: 3080 2119 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2523 4821 5181 Size: 274 80 33 51 1443 546 2298 360 Cuts at: 5181 6000 6998 8710 96 Size: 819 998 1712 138 Fragments arranged by size: 2298 1712 1443 998 819 546 360 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4338 4370 8712 8744 4338 Size: 32 4342 32 4346 Fragments arranged by size: 4346 4342 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2162 3463 1316 Size: 846 1301 6605 Fragments arranged by size: 6605 1301 846 XbaI T'CTAG_A Cuts at: 2275 6088 2275 Size: 3813 4939 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5740 5740 Size: 8752 XhoI C'TCGA_G Cuts at: 1943 2767 1943 Size: 824 7928 XmaI C'CCGG_G Cuts at: 1870 6077 1870 Size: 4207 4545 XmnI GAAnn'nnTTC Cuts at: 2899 3067 3138 3412 4479 4989 5776 6881 2899 Size: 168 71 274 1067 510 787 1105 4770 Fragments arranged by size: 4770 1105 1067 787 510 274 168 71 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6560 276 Size: 53 83 186 936 5026 2468 Fragments arranged by size: 5026 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI