Restriction map for
pFN21AE2704
(Linear) (Six-base) MAPSORT of: KIEE2704.seq Check: 2458 from: 1 to: 4047
KIEE2704.orf
With 182 enzymes: SgfI *
September 21, 2017 11:26 ..
AccI GT'mk_AC
Cuts at: 0 346 954 1381 4047
Size: 346 608 427 2666
Fragments arranged by size:
2666 608 427 346
AclI AA'CG_TT
Cuts at: 0 377 4047
Size: 377 3670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1241 1499 2461 2909 3043 3070 3212 3394
Size: 1241 258 962 448 134 27 142 182
Cuts at: 3394 3689 3764 3989 4047
Size: 295 75 225 58
Fragments arranged by size:
1241 962 448 295 258 225 182 142
134 75 58 27
AleI CACnn'nnGTG
Cuts at: 0 3736 4047
Size: 3736 311
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2822 2854 3670 3702 4047
Size: 2822 32 816 32 345
Fragments arranged by size:
2822 816 345 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 161 3967 4047
Size: 161 3806 80
Fragments arranged by size:
3806 161 80
ApoI r'AATT_y
Cuts at: 0 92 198 240 697 841 1520 1577 2230
Size: 92 106 42 457 144 679 57 653
Cuts at: 2230 2417 2513 2808 4047
Size: 187 96 295 1239
Fragments arranged by size:
1239 679 653 457 295 187 144 106
96 92 57 42
AseI AT'TA_AT
Cuts at: 0 302 4047
Size: 302 3745
AvaI C'yCGr_G
Cuts at: 0 762 2655 2942 3762 4047
Size: 762 1893 287 820 285
Fragments arranged by size:
1893 820 762 287 285
BamHI G'GATC_C
Cuts at: 0 3455 4047
Size: 3455 592
BanI G'GyrC_C
Cuts at: 0 716 4047
Size: 716 3331
BanII G_rGCy'C
Cuts at: 0 80 87 763 2879 4047
Size: 80 7 676 2116 1168
Fragments arranged by size:
2116 1168 676 80 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 632 1622 1835 2015 2915 3490 3727 4047
Size: 632 990 213 180 900 575 237 320
Fragments arranged by size:
990 900 632 575 320 237 213 180
BbvCI CC'TCA_GC
Cuts at: 0 3092 3098 3641 4047
Size: 3092 6 543 406
Fragments arranged by size:
3092 543 406 6
BclI T'GATC_A
Cuts at: 0 3878 4047
Size: 3878 169
BglI GCCn_nnn'nGGC
Cuts at: 0 3576 4047
Size: 3576 471
BglII A'GATC_T
Cuts at: 0 438 1260 1783 3262 4047
Size: 438 822 523 1479 785
Fragments arranged by size:
1479 822 785 523 438
Bme1580I G_kGCm'C
Cuts at: 0 2655 3698 4047
Size: 2655 1043 349
Fragments arranged by size:
2655 1043 349
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3587 4047
Size: 3587 460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 615 1447 2078 2925 3672 4047
Size: 455 160 832 631 847 747 375
Fragments arranged by size:
847 832 747 631 455 375 160
Bpu10I CC'TnA_GC
Cuts at: 0 2324 2842 3092 3098 3641 4047
Size: 2324 518 250 6 543 406
Fragments arranged by size:
2324 543 518 406 250 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1330 2076 2315 4047
Size: 1330 746 239 1732
Fragments arranged by size:
1732 1330 746 239
BsaBI GATnn'nnATC
Cuts at: 0 4023 4047
Size: 4023 24
BsaHI Gr'CG_yC
Cuts at: 0 320 2140 4047
Size: 320 1820 1907
Fragments arranged by size:
1907 1820 320
BsaWI w'CCGG_w
Cuts at: 0 1697 4047
Size: 1697 2350
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3526 3556 3670 3700 3956 3986 4047
Size: 3526 30 114 30 256 30 61
Fragments arranged by size:
3526 256 114 61 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 698 774 1177 2695 2698 2971 2976 3115
Size: 698 76 403 1518 3 273 5 139
Cuts at: 3115 3136 3366 3675 4047
Size: 21 230 309 372
Fragments arranged by size:
1518 698 403 372 309 273 230 139
76 21 5 3
BseYI C'CCAG_C
Cuts at: 0 419 525 1617 2223 2663 4047
Size: 419 106 1092 606 440 1384
Fragments arranged by size:
1384 1092 606 440 419 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1853 4047
Size: 1853 2194
BsiHKAI G_wGCw'C
Cuts at: 0 80 87 117 1651 4047
Size: 80 7 30 1534 2396
Fragments arranged by size:
2396 1534 80 30 7
Bsp1286I G_dGCh'C
Cuts at: 0 80 87 117 763 1651 2655 2879 3698
Size: 80 7 30 646 888 1004 224 819
Cuts at: 3698 4047
Size: 349
Fragments arranged by size:
1004 888 819 646 349 224 80 30
7
BspHI T'CATG_A
Cuts at: 0 2517 2988 3509 4047
Size: 2517 471 521 538
Fragments arranged by size:
2517 538 521 471
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2206 4047
Size: 2206 1841
BsrBI CCG'CTC
Cuts at: 0 2688 3150 4047
Size: 2688 462 897
Fragments arranged by size:
2688 897 462
BsrDI GCAATG_nn'
Cuts at: 0 2535 4047
Size: 2535 1512
BssSI C'ACGA_G
Cuts at: 0 1667 4047
Size: 1667 2380
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4007 4047
Size: 4007 40
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2143 2820 3738 4047
Size: 2143 677 918 309
Fragments arranged by size:
2143 918 677 309
BstYI r'GATC_y
Cuts at: 0 438 1260 1783 2095 2192 3262 3355 3455
Size: 438 822 523 312 97 1070 93 100
Cuts at: 3455 4047
Size: 592
Fragments arranged by size:
1070 822 592 523 438 312 100 97
93
Bsu36I CC'TnA_GG
Cuts at: 0 3960 4047
Size: 3960 87
BtgI C'CryG_G
Cuts at: 0 2318 4047
Size: 2318 1729
BtsI GCAGTG_nn'
Cuts at: 0 3474 4047
Size: 3474 573
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1835 1870 4047
Size: 1835 35 2177
Fragments arranged by size:
2177 1835 35
DraIII CAC_nnn'GTG
Cuts at: 0 2349 4047
Size: 2349 1698
EaeI y'GGCC_r
Cuts at: 0 3740 4033 4047
Size: 3740 293 14
Fragments arranged by size:
3740 293 14
EarI CTCTTCn'nnn_
Cuts at: 0 67 473 1274 1487 2143 2765 2948 3059
Size: 67 406 801 213 656 622 183 111
Cuts at: 3059 3348 3418 3510 3960 4047
Size: 289 70 92 450 87
Fragments arranged by size:
801 656 622 450 406 289 213 183
111 92 87 70 67
EcoICRI GAG'CTC
Cuts at: 0 78 85 4047
Size: 78 7 3962
Fragments arranged by size:
3962 78 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 615 1241 1447 1499 2078 2461 2909
Size: 455 160 626 206 52 579 383 448
Cuts at: 2909 2925 3043 3070 3212 3394 3672 3689 3764
Size: 16 118 27 142 182 278 17 75
Cuts at: 3764 3989 4047
Size: 225 58
Fragments arranged by size:
626 579 455 448 383 278 225 206
182 160 142 118 75 58 52 27
17 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2731 4047
Size: 2731 1316
EcoRI G'AATT_C
Cuts at: 0 2513 2808 4047
Size: 2513 295 1239
Fragments arranged by size:
2513 1239 295
EcoRV GAT'ATC
Cuts at: 0 792 4047
Size: 792 3255
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 439 1271 2549 4047
Size: 439 832 1278 1498
Fragments arranged by size:
1498 1278 832 439
HaeII r_GCGC'y
Cuts at: 0 2367 3135 4047
Size: 2367 768 912
Fragments arranged by size:
2367 912 768
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 99 131 452 484 865 897 1348 1380
Size: 99 32 321 32 381 32 451 32
Cuts at: 1380 2032 2064 3271 3303 3956 3988 4047
Size: 652 32 1207 32 653 32 59
Fragments arranged by size:
1207 653 652 451 381 321 99 59
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1721 3480 4047
Size: 1721 1759 567
Fragments arranged by size:
1759 1721 567
HindIII A'AGCT_T
Cuts at: 0 307 389 744 1902 4047
Size: 307 82 355 1158 2145
Fragments arranged by size:
2145 1158 355 307 82
HpaI GTT'AAC
Cuts at: 0 1721 4047
Size: 1721 2326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 2665 3483 4047
Size: 22 2643 818 564
Fragments arranged by size:
2643 818 564 22
MscI TGG'CCA
Cuts at: 0 3742 4047
Size: 3742 305
MslI CAynn'nnrTG
Cuts at: 0 130 1654 3736 4047
Size: 130 1524 2082 311
Fragments arranged by size:
2082 1524 311 130
MspA1I CmG'CkG
Cuts at: 0 1152 3452 3645 3709 3804 4047
Size: 1152 2300 193 64 95 243
Fragments arranged by size:
2300 1152 243 193 95 64
NcoI C'CATG_G
Cuts at: 0 2318 4047
Size: 2318 1729
NdeI CA'TA_TG
Cuts at: 0 3835 4047
Size: 3835 212
NspI r_CATG'y
Cuts at: 0 2859 3675 4047
Size: 2859 816 372
Fragments arranged by size:
2859 816 372
PasI CC'CwG_GG
Cuts at: 0 3891 4047
Size: 3891 156
PflMI CCAn_nnn'nTGG
Cuts at: 0 3560 4047
Size: 3560 487
PfoI T'CCnGG_A
Cuts at: 0 432 4047
Size: 432 3615
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3325 3357 3670 3702 4047
Size: 3325 32 313 32 345
Fragments arranged by size:
3325 345 313 32 32
PshAI GACnn'nnGTC
Cuts at: 0 3626 4047
Size: 3626 421
PsiI TTA'TAA
Cuts at: 0 866 4047
Size: 866 3181
PspXI vC'TCGA_Gb
Cuts at: 0 762 4047
Size: 762 3285
PstI C_TGCA'G
Cuts at: 0 166 1268 1355 2735 4047
Size: 166 1102 87 1380 1312
Fragments arranged by size:
1380 1312 1102 166 87
PvuII CAG'CTG
Cuts at: 0 1152 3452 3645 3709 3804 4047
Size: 1152 2300 193 64 95 243
Fragments arranged by size:
2300 1152 243 193 95 64
SacI G_AGCT'C
Cuts at: 0 80 87 4047
Size: 80 7 3960
Fragments arranged by size:
3960 80 7
SapI GCTCTTCn'nnn_
Cuts at: 0 3059 3960 4047
Size: 3059 901 87
Fragments arranged by size:
3059 901 87
ScaI AGT'ACT
Cuts at: 0 2818 4047
Size: 2818 1229
SfcI C'TryA_G
Cuts at: 0 162 442 1264 1351 1382 2731 4047
Size: 162 280 822 87 31 1349 1316
Fragments arranged by size:
1349 1316 822 280 162 87 31
SmlI C'TyrA_G
Cuts at: 0 762 1309 2091 2330 4047
Size: 762 547 782 239 1717
Fragments arranged by size:
1717 782 762 547 239
SspI AAT'ATT
Cuts at: 0 2490 3316 4047
Size: 2490 826 731
Fragments arranged by size:
2490 826 731
StuI AGG'CCT
Cuts at: 0 3186 4047
Size: 3186 861
StyI C'CwwG_G
Cuts at: 0 712 2318 2997 3574 4047
Size: 712 1606 679 577 473
Fragments arranged by size:
1606 712 679 577 473
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2655 4047
Size: 2655 1392
TatI w'GTAC_w
Cuts at: 0 518 2816 3176 3995 4047
Size: 518 2298 360 819 52
Fragments arranged by size:
2298 819 518 360 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2333 2365 4047
Size: 2333 32 1682
Fragments arranged by size:
2333 1682 32
Tth111I GACn'n_nGTC
Cuts at: 0 157 1458 4047
Size: 157 1301 2589
Fragments arranged by size:
2589 1301 157
XbaI T'CTAG_A
Cuts at: 0 270 4047
Size: 270 3777
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3735 4047
Size: 3735 312
XhoI C'TCGA_G
Cuts at: 0 762 4047
Size: 762 3285
XmnI GAAnn'nnTTC
Cuts at: 0 894 1062 1133 1407 2474 2984 3771 4047
Size: 894 168 71 274 1067 510 787 276
Fragments arranged by size:
1067 894 787 510 276 274 168 71
Enzymes that do cut:
AccI AclI AcuI AleI AloI AlwNI ApoI AseI
AvaI BamHI BanI BanII BbsI BbvCI BclI BglI
BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
HpaI MmeI MscI MslI MspA1I NcoI NdeI NspI
PasI PflMI PfoI PpiI PshAI PsiI PspXI PstI
PvuII SacI SapI ScaI SfcI SmlI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AlfI ApaI ApaLI AscI AsiSI AvrII BaeI BbeI
BcgI BciVI BfrBI BlpI BmgBI BmrI BmtI BsaI
BsaAI BsiEI BsiWI BsmI BsmBI BspEI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I BtgZI ClaI DraI DrdI
EagI EciI EcoO109I FseI FspI FspAI KasI KpnI
MfeI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpuMI PspOMI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI XmaI ZraI