Restriction map for
pFN21AE2650
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflIII AgeI AhdI
AlwI Aor51HI ApaI AscI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BclI
BcnI BlnI BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CfoI Cfr10I Cfr13I ClaI CpoI
DraII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RsrII
SacII SalI Sau96I SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspI Tth111I Van91I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1511 GTAGAC GT^[AC][GT]AC
AflII 1756 CTTAAG C^TTAAG
Alw44I 1585 GTGCAC G^TGCAC
AlwNI 92 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1585 GTGCAC G^TGCAC
AseI 1864 ATTAAT AT^TAAT
AsnI 1864 ATTAAT AT^TAAT
BbvI 736 GCAGC GCAGC(8/12)
BciVI 2025 GGATAC GTATCC(6/5)
BfrI 1756 CTTAAG C^TTAAG
BlpI 1096 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1096 GCTAAGC GC^T[ACGT]AGC
BsaAI 1508 CACGTA [CT]AC^GT[GA]
BsaBI 553 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 114 TCCGGT [AT]^CCGG[AT]
BsgI 1789 CTGCAC GTGCAG(16/14)
BsmFI 1547 GGGAC GGGAC(10/14)
BsmI 1713 GCATTC GAATGC(1/-1)
Bsp1407I 21 TGTACA T^GTACA
BspHI 1695 TCATGA T^CATGA
BsrGI 21 TGTACA T^GTACA
Cac8I 907 GCCAGC GC[ACGT]^[ACGT]GC
CelII 1096 GCTAAGC GC^T[ACGT]AGC
DraIII 1576 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 2355 CCAAGG C^C[AT][AT]GG
Fnu4HI 736 GCAGC GC^[ACGT]GC
HapII 115 CCGG C^CGG
HpaI 87 GTTAAC GTT^AAC
HpaII 115 CCGG C^CGG
ItaI 736 GCAGC GC^[ACGT]GC
MamI 553 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1613 CAGCTG C[AC]G^C[GT]G
MspI 115 CCGG C^CGG
NdeI 2554 CATATG CA^TATG
PshBI 1864 ATTAAT AT^TAAT
PvuII 1613 CAGCTG CAG^CTG
RcaI 1695 TCATGA T^CATGA
SacI 1455 GAGCTC GAGCT^C
SapI 1222 GAAGAGC GCTCTTC(1/4)
ScaI 723 AGTACT AGT^ACT
SexAI 1577 ACCAGGT A^CC[AT]GGT
SfcI 1415 CTGTAG C^T[GA][CT]AG
SpeI 16 ACTAGT A^CTAGT
SspBI 21 TGTACA T^GTACA
StyI 2355 CCAAGG C^C[AT][AT]GG
SwaI 849 ATTTAAAT ATTT^AAAT
TaqI 2232 TCGA T^CGA
TseI 736 GCAGC G^C[AT]GC
TthHB8I 2232 TCGA T^CGA
XcmI 1382 CCAGTGATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspHI 1455 GAGCTC G[AT]GC[AT]^C
1585 GTGCAC
BanII 1225 GAGCCC G[GA]GC[CT]^C
1455 GAGCTC
BbsI 787 GTCTTC GAAGAC(2/6)
2428 GTCTTC
BglI 1227 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2479 GCCAAAATGGC
BglII 882 AGATCT A^GATCT
2086 AGATCT
BpuAI 787 GTCTTC GAAGAC(2/6)
2428 GTCTTC
BsiHKAI 1455 GAGCTC G[AT]GC[AT]^C
1585 GTGCAC
BsiYI 287 CCAGTAGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
951 CCCACAAAAGG
BslI 287 CCAGTAGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
951 CCCACAAAAGG
BsmAI 1123 GAGAC GTCTC(1/5)
2259 GAGAC
BstYI 882 AGATCT [GA]^GATC[CT]
2086 AGATCT
HincII 87 GTTAAC GT[CT]^[GA]AC
2373 GTTGAC
HindII 87 GTTAAC GT[CT]^[GA]AC
2373 GTTGAC
MaeII 140 ACGT A^CGT
1509 ACGT
MflI 882 AGATCT [GA]^GATC[CT]
2086 AGATCT
StuI 1169 AGGCCT AGG^CCT
1283 AGGCCT
Tsp45I 1680 GTCAC ^GT[GC]AC
1747 GTCAC
XhoII 882 AGATCT [GA]^GATC[CT]
2086 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AcsI AfaI AluI AocI ApoI
BfaI BmyI BpmI BsaJI BseRI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Bsu36I DdeI DpnII DraI
EarI Eco81I EcoRI EcoRII FokI
HaeIII HinfI HphI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
PleI RsaI Sau3AI ScrFI SfaNI
SmlI TfiI Tru9I Tsp509I TspRI
XbaI