(Circular) (Six-base) MAPSORT of: pFN21AE2650.seq Check: 9712 from: 1 to: 7300 pFN21AE2650 7300 bp With 182 enzymes: SgfI * November 16, 2016 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5110 278 Size: 53 83 186 936 3574 2468 Fragments arranged by size: 3574 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3517 4643 1937 Size: 1580 1126 4594 Fragments arranged by size: 4594 1580 1126 Acc65I G'GTAC_C Cuts at: 4621 4621 Size: 7300 AclI AA'CG_TT Cuts at: 1563 5427 5800 1563 Size: 3864 373 3063 Fragments arranged by size: 3864 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3252 3306 5363 6747 7015 1632 Size: 1620 54 2057 1384 268 1917 Fragments arranged by size: 2057 1917 1620 1384 268 54 AflII C'TTAA_G Cuts at: 820 1017 3761 820 Size: 197 2744 4359 Fragments arranged by size: 4359 2744 197 AflIII A'CryG_T Cuts at: 1203 6199 7176 1203 Size: 4996 977 1327 Fragments arranged by size: 4996 1327 977 AgeI A'CCGG_T Cuts at: 6131 6131 Size: 7300 AhdI GACnn_n'nnGTC Cuts at: 6029 6029 Size: 7300 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7300 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7300 AlwNI CAG_nnn'CTG Cuts at: 2102 5043 6615 2102 Size: 2941 1572 2787 Fragments arranged by size: 2941 2787 1572 ApaLI G'TGCA_C Cuts at: 3590 5357 6513 3590 Size: 1767 1156 4377 Fragments arranged by size: 4377 1767 1156 ApoI r'AATT_y Cuts at: 1493 2036 2184 2278 2398 2582 2621 2651 2828 Size: 543 148 94 120 184 39 30 177 Cuts at: 2828 2906 2997 3528 3730 3904 3943 4019 4122 Size: 78 91 531 202 174 39 76 103 Cuts at: 4122 4292 4300 4538 4609 4835 4987 7096 1493 Size: 170 8 238 71 226 152 2109 1697 Fragments arranged by size: 2109 1697 543 531 238 226 202 184 177 174 170 152 148 120 103 94 91 78 76 71 39 39 30 8 AseI AT'TA_AT Cuts at: 160 1032 3870 5854 160 Size: 872 2838 1984 1606 Fragments arranged by size: 2838 1984 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7300 AvaI C'yCGr_G Cuts at: 1870 1943 4625 1870 Size: 73 2682 4545 Fragments arranged by size: 4545 2682 73 BamHI G'GATC_C Cuts at: 4630 4997 4630 Size: 367 6933 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4621 6076 618 Size: 325 217 627 2834 1455 1842 Fragments arranged by size: 2834 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3234 3464 4619 1391 Size: 1843 230 1155 4072 Fragments arranged by size: 4072 1843 1155 230 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2785 4426 928 Size: 971 886 1641 3802 Fragments arranged by size: 3802 1641 971 886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5491 5525 1903 Size: 34 3554 34 3678 Fragments arranged by size: 3678 3554 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4024 5192 6402 4024 Size: 1168 1210 4922 Fragments arranged by size: 4922 1210 1168 BclI T'GATC_A Cuts at: 1548 1548 Size: 7300 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3238 4490 5911 Size: 107 122 71 631 605 1566 1252 1421 Cuts at: 5911 136 Size: 1525 Fragments arranged by size: 1566 1525 1421 1252 631 605 122 107 71 BglII A'GATC_T Cuts at: 2887 4091 7295 2887 Size: 1204 3204 2892 Fragments arranged by size: 3204 2892 1204 BlpI GC'TnA_GC Cuts at: 3102 4717 3102 Size: 1615 5685 Bme1580I G_kGCm'C Cuts at: 1790 3594 3627 5361 6517 1790 Size: 1804 33 1734 1156 2573 Fragments arranged by size: 2573 1804 1734 1156 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7300 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4592 5031 5989 Size: 416 53 83 399 24 3175 439 958 Cuts at: 5989 442 Size: 1753 Fragments arranged by size: 3175 1753 958 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7300 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2235 4287 2235 Size: 2052 5248 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2275 2677 3066 3233 3263 5960 1239 Size: 1036 402 389 167 30 2697 2579 Fragments arranged by size: 2697 2579 1036 402 389 167 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3088 3117 3874 4238 4591 5425 6290 6588 3088 Size: 29 757 364 353 834 865 298 3800 Fragments arranged by size: 3800 865 834 757 364 353 298 29 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5963 882 Size: 617 4464 2219 Fragments arranged by size: 4464 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3515 5091 493 Size: 711 2311 1576 2702 Fragments arranged by size: 2702 2311 1576 711 BsaBI GATnn'nnATC Cuts at: 2562 7294 2562 Size: 4732 2568 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5107 5489 6124 275 Size: 53 83 186 936 3574 382 635 1451 Fragments arranged by size: 3574 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2119 5733 6131 6405 6552 6963 2119 Size: 3614 398 274 147 411 2456 Fragments arranged by size: 3614 2456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7075 Fragments arranged by size: 7075 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 2690 3125 3173 3230 3233 3236 3260 Size: 1033 471 435 48 57 3 3 24 Cuts at: 3260 3293 3317 3455 3908 4100 1186 Size: 33 24 138 453 192 4386 Fragments arranged by size: 4386 1033 471 453 435 192 138 57 48 33 24 24 3 3 BseYI C'CCAG_C Cuts at: 6503 6999 6503 Size: 496 6804 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3779 1163 Size: 573 2043 4684 Fragments arranged by size: 4684 2043 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4750 5511 6539 1540 Size: 275 186 2749 761 1028 2301 Fragments arranged by size: 2749 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3464 3594 5361 5446 6517 3464 Size: 130 1767 85 1071 4247 Fragments arranged by size: 4247 1767 1071 130 85 BsmI GAATG_Cn' Cuts at: 3718 4811 4904 3718 Size: 1093 93 6114 Fragments arranged by size: 6114 1093 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3234 3464 3594 3627 4619 5361 5446 Size: 399 1444 230 130 33 992 742 85 Cuts at: 5446 6517 1391 Size: 1071 2174 Fragments arranged by size: 2174 1444 1071 992 742 399 230 130 85 33 BspEI T'CCGG_A Cuts at: 6963 6963 Size: 7300 BspHI T'CATG_A Cuts at: 3700 5189 6919 3700 Size: 1489 1730 4081 Fragments arranged by size: 4081 1730 1489 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4655 844 Size: 3811 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5187 1394 Size: 429 3364 3507 Fragments arranged by size: 3507 3364 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2575 2786 4435 5795 5969 65 Size: 1183 1327 211 1649 1360 174 1396 Fragments arranged by size: 1649 1396 1360 1327 1183 211 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5944 6131 1700 Size: 110 4134 187 2869 Fragments arranged by size: 4134 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2026 96 Size: 1930 5370 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7300 BssSI C'ACGA_G Cuts at: 5360 6372 5360 Size: 1012 6288 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7300 BstBI TT'CG_AA Cuts at: 6104 6104 Size: 7300 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7300 BstYI r'GATC_y Cuts at: 1972 2887 4091 4630 4997 5382 5399 6851 6937 Size: 915 1204 539 367 385 17 1452 86 Cuts at: 6937 6949 7295 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1204 915 539 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2268 3178 3205 3235 3292 2268 Size: 910 27 30 57 6276 Fragments arranged by size: 6276 910 57 30 27 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6748 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6561 Fragments arranged by size: 6561 683 56 BtsI GCAGTG_nn' Cuts at: 4816 5610 5630 4816 Size: 794 20 6486 Fragments arranged by size: 6486 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7300 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7265 DraI TTT'AAA Cuts at: 2133 2410 2685 2857 4604 2133 Size: 277 275 172 1747 4829 Fragments arranged by size: 4829 1747 277 275 172 DraIII CAC_nnn'GTG Cuts at: 3586 3586 Size: 7300 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6307 809 Size: 507 4991 1802 Fragments arranged by size: 4991 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4747 5636 7265 Size: 54 1425 50 275 18 2917 889 1629 Cuts at: 7265 8 Size: 43 Fragments arranged by size: 2917 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4747 1537 Size: 275 2935 4090 Fragments arranged by size: 4090 2935 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3135 3165 3222 3255 3303 3603 4339 5230 Size: 1782 30 57 33 48 300 736 891 Cuts at: 5230 1353 Size: 3423 Fragments arranged by size: 3423 1782 891 736 300 57 48 33 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5887 6261 6407 387 Size: 1064 138 4298 374 146 1280 Fragments arranged by size: 4298 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3462 3462 Size: 7300 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2275 2677 3066 3233 3252 3263 3306 Size: 393 643 402 389 167 19 11 43 Cuts at: 3306 5363 5960 6747 7015 1239 Size: 2057 597 787 268 1524 Fragments arranged by size: 2057 1524 787 643 597 402 393 389 268 167 43 19 11 EcoRI G'AATT_C Cuts at: 2278 2997 4019 4122 4300 4609 4987 2278 Size: 719 1022 103 178 309 378 4591 Fragments arranged by size: 4591 1022 719 378 309 178 103 FspI TGC'GCA Cuts at: 5806 5806 Size: 7300 HaeII r_GCGC'y Cuts at: 1678 6447 1678 Size: 4769 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2738 2770 3275 3307 3419 Size: 32 135 32 1166 32 505 32 112 Cuts at: 3419 3451 4076 4108 5941 5973 6015 6047 1373 Size: 32 625 32 1833 32 42 32 2626 Fragments arranged by size: 2626 1833 1166 625 505 135 112 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2094 4380 4644 4890 669 Size: 988 281 156 2286 264 246 3079 Fragments arranged by size: 3079 2286 988 281 264 246 156 HindIII A'AGCT_T Cuts at: 748 748 Size: 7300 HpaI GTT'AAC Cuts at: 2094 4890 2094 Size: 2796 4504 KpnI G_GTAC'C Cuts at: 4625 4625 Size: 7300 MfeI C'AATT_G Cuts at: 4899 4899 Size: 7300 MluI A'CGCG_T Cuts at: 7176 7176 Size: 7300 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3255 6414 6598 1305 Size: 1950 3159 184 2007 Fragments arranged by size: 3159 2007 1950 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5478 Fragments arranged by size: 5478 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2075 2857 3924 4286 5258 5617 5776 Size: 648 909 782 1067 362 972 359 159 Cuts at: 5776 518 Size: 2042 Fragments arranged by size: 2042 1067 972 909 782 648 362 359 159 MspA1I CmG'CkG Cuts at: 1606 3620 4716 5393 6541 6786 1606 Size: 2014 1096 677 1148 245 2120 Fragments arranged by size: 2120 2014 1148 1096 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6748 NdeI CA'TA_TG Cuts at: 387 4560 387 Size: 4173 3127 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7300 NotI GC'GGCC_GC Cuts at: 4747 4747 Size: 7300 NspI r_CATG'y Cuts at: 1132 1229 4658 6203 1132 Size: 97 3429 1545 2229 Fragments arranged by size: 3429 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2488 1348 Size: 1140 6160 PciI A'CATG_T Cuts at: 6199 6199 Size: 7300 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7300 PmeI GTTT'AAAC Cuts at: 4604 4604 Size: 7300 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5369 5401 6882 6914 5369 Size: 32 1481 32 5755 Fragments arranged by size: 5755 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7300 PsiI TTA'TAA Cuts at: 4870 4870 Size: 7300 PstI C_TGCA'G Cuts at: 830 4652 830 Size: 3822 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7300 PvuII CAG'CTG Cuts at: 3620 3620 Size: 7300 SacI G_AGCT'C Cuts at: 3464 3464 Size: 7300 SalI G'TCGA_C Cuts at: 1936 4642 1936 Size: 2706 4594 SapI GCTCTTCn'nnn_ Cuts at: 3222 3222 Size: 7300 SbfI CC_TGCA'GG Cuts at: 4652 4652 Size: 7300 ScaI AGT'ACT Cuts at: 2730 5548 7260 2730 Size: 2818 1712 2770 Fragments arranged by size: 2818 2770 1712 SexAI A'CCwGG_T Cuts at: 3582 3582 Size: 7300 SfcI C'TryA_G Cuts at: 826 1045 3420 4648 5783 6464 6655 826 Size: 219 2375 1228 1135 681 191 1471 Fragments arranged by size: 2375 1471 1228 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7300 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7300 SmaI CCC'GGG Cuts at: 1872 4627 1872 Size: 2755 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3067 3132 3761 3853 4217 4570 Size: 197 926 1124 65 629 92 364 353 Cuts at: 4570 5404 6305 6567 820 Size: 834 901 262 1553 Fragments arranged by size: 1553 1124 926 901 834 629 364 353 262 197 92 65 SnaBI TAC'GTA Cuts at: 493 5091 493 Size: 4598 2702 SpeI A'CTAG_T Cuts at: 152 2021 152 Size: 1869 5431 SphI G_CATG'C Cuts at: 1132 4658 1132 Size: 3526 3774 SspI AAT'ATT Cuts at: 5 52 5224 5 Size: 47 5172 2081 Fragments arranged by size: 5172 2081 47 StuI AGG'CCT Cuts at: 1517 3176 3290 1517 Size: 1659 114 5527 Fragments arranged by size: 5527 1659 114 StyI C'CwwG_G Cuts at: 513 1065 4360 4739 513 Size: 552 3295 379 3074 Fragments arranged by size: 3295 3074 552 379 SwaI ATTT'AAAT Cuts at: 2857 2857 Size: 7300 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5327 5344 5497 5682 6127 325 Size: 1255 3747 17 153 185 445 1498 Fragments arranged by size: 3747 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2026 2728 2950 Size: 274 80 33 51 1443 49 702 222 Cuts at: 2950 5546 7258 96 Size: 2596 1712 138 Fragments arranged by size: 2596 1712 1443 702 274 222 138 80 51 49 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4291 4323 7260 7292 4291 Size: 32 2937 32 4299 Fragments arranged by size: 4299 2937 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7300 XbaI T'CTAG_A Cuts at: 4000 4094 4334 4636 4000 Size: 94 240 302 6664 Fragments arranged by size: 6664 302 240 94 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3394 3394 Size: 7300 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7300 XmaI C'CCGG_G Cuts at: 1870 4625 1870 Size: 2755 4545 XmnI GAAnn'nnTTC Cuts at: 5429 5429 Size: 7300 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5108 276 Size: 53 83 186 936 3574 2468 Fragments arranged by size: 3574 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI