(Linear) (Six-base) MAPSORT of: KIEE2650.seq Check: 8017 from: 1 to: 2595 KIEE2650.orf With 182 enzymes: SgfI * November 16, 2016 14:56 .. AccI GT'mk_AC Cuts at: 0 1512 2595 Size: 1512 1083 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1247 1301 2595 Size: 1247 54 1294 Fragments arranged by size: 1294 1247 54 AflII C'TTAA_G Cuts at: 0 1756 2595 Size: 1756 839 AlwNI CAG_nnn'CTG Cuts at: 0 97 2595 Size: 97 2498 ApaLI G'TGCA_C Cuts at: 0 1585 2595 Size: 1585 1010 ApoI r'AATT_y Cuts at: 0 31 179 273 393 577 616 646 823 Size: 31 148 94 120 184 39 30 177 Cuts at: 823 901 992 1523 1725 1899 1938 2014 2117 Size: 78 91 531 202 174 39 76 103 Cuts at: 2117 2287 2295 2533 2595 Size: 170 8 238 62 Fragments arranged by size: 531 238 202 184 177 174 170 148 120 103 94 91 78 76 62 39 39 31 30 8 AseI AT'TA_AT Cuts at: 0 1865 2595 Size: 1865 730 BanII G_rGCy'C Cuts at: 0 1229 1459 2595 Size: 1229 230 1136 Fragments arranged by size: 1229 1136 230 BbsI GAAGACnn'nnnn_ Cuts at: 0 780 2421 2595 Size: 780 1641 174 Fragments arranged by size: 1641 780 174 BciVI GTATCCnnnnn_n' Cuts at: 0 2019 2595 Size: 2019 576 BglI GCCn_nnn'nGGC Cuts at: 0 1233 2485 2595 Size: 1233 1252 110 Fragments arranged by size: 1252 1233 110 BglII A'GATC_T Cuts at: 0 882 2086 2595 Size: 882 1204 509 Fragments arranged by size: 1204 882 509 BlpI GC'TnA_GC Cuts at: 0 1097 2595 Size: 1097 1498 Bme1580I G_kGCm'C Cuts at: 0 1589 1622 2595 Size: 1589 33 973 Fragments arranged by size: 1589 973 33 BmrI ACTGGGnnnn_n' Cuts at: 0 2587 2595 Size: 2587 8 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 230 2282 2595 Size: 230 2052 313 Fragments arranged by size: 2052 313 230 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 270 672 1061 1228 1258 2595 Size: 270 402 389 167 30 1337 Fragments arranged by size: 1337 402 389 270 167 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1083 1112 1869 2233 2586 2595 Size: 1083 29 757 364 353 9 Fragments arranged by size: 1083 757 364 353 29 9 BsaAI yAC'GTr Cuts at: 0 1510 2595 Size: 1510 1085 BsaBI GATnn'nnATC Cuts at: 0 557 2595 Size: 557 2038 BsaWI w'CCGG_w Cuts at: 0 114 2595 Size: 114 2481 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 214 685 1120 1168 1225 1228 1231 1255 Size: 214 471 435 48 57 3 3 24 Cuts at: 1255 1288 1312 1450 1903 2095 2595 Size: 33 24 138 453 192 500 Fragments arranged by size: 500 471 453 435 214 192 138 57 48 33 24 24 3 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1774 2595 Size: 1774 821 BsiHKAI G_wGCw'C Cuts at: 0 1459 1589 2595 Size: 1459 130 1006 Fragments arranged by size: 1459 1006 130 BsmI GAATG_Cn' Cuts at: 0 1713 2595 Size: 1713 882 Bsp1286I G_dGCh'C Cuts at: 0 1229 1459 1589 1622 2595 Size: 1229 230 130 33 973 Fragments arranged by size: 1229 973 230 130 33 BspHI T'CATG_A Cuts at: 0 1695 2595 Size: 1695 900 BsrDI GCAATG_nn' Cuts at: 0 570 781 2430 2595 Size: 570 211 1649 165 Fragments arranged by size: 1649 570 211 165 BsrGI T'GTAC_A Cuts at: 0 21 2595 Size: 21 2574 BstYI r'GATC_y Cuts at: 0 882 2086 2595 Size: 882 1204 509 Fragments arranged by size: 1204 882 509 Bsu36I CC'TnA_GG Cuts at: 0 263 1173 1200 1230 1287 2595 Size: 263 910 27 30 57 1308 Fragments arranged by size: 1308 910 263 57 30 27 DraI TTT'AAA Cuts at: 0 128 405 680 852 2595 Size: 128 277 275 172 1743 Fragments arranged by size: 1743 277 275 172 128 DraIII CAC_nnn'GTG Cuts at: 0 1581 2595 Size: 1581 1014 EarI CTCTTCn'nnn_ Cuts at: 0 1130 1160 1217 1250 1298 1598 2334 2595 Size: 1130 30 57 33 48 300 736 261 Fragments arranged by size: 1130 736 300 261 57 48 33 30 EcoICRI GAG'CTC Cuts at: 0 1457 2595 Size: 1457 1138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 270 672 1061 1228 1247 1258 1301 2595 Size: 270 402 389 167 19 11 43 1294 Fragments arranged by size: 1294 402 389 270 167 43 19 11 EcoRI G'AATT_C Cuts at: 0 273 992 2014 2117 2295 2595 Size: 273 719 1022 103 178 300 Fragments arranged by size: 1022 719 300 273 178 103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 733 765 1270 1302 1414 1446 2071 2103 Size: 733 32 505 32 112 32 625 32 Cuts at: 2103 2595 Size: 492 Fragments arranged by size: 733 625 505 492 112 32 32 32 32 HincII GTy'rAC Cuts at: 0 89 2375 2595 Size: 89 2286 220 Fragments arranged by size: 2286 220 89 HpaI GTT'AAC Cuts at: 0 89 2595 Size: 89 2506 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1250 2595 Size: 1250 1345 MslI CAynn'nnrTG Cuts at: 0 70 852 1919 2281 2595 Size: 70 782 1067 362 314 Fragments arranged by size: 1067 782 362 314 70 MspA1I CmG'CkG Cuts at: 0 1615 2595 Size: 1615 980 NdeI CA'TA_TG Cuts at: 0 2555 2595 Size: 2555 40 PasI CC'CwG_GG Cuts at: 0 483 2595 Size: 483 2112 PvuII CAG'CTG Cuts at: 0 1615 2595 Size: 1615 980 SacI G_AGCT'C Cuts at: 0 1459 2595 Size: 1459 1136 SapI GCTCTTCn'nnn_ Cuts at: 0 1217 2595 Size: 1217 1378 ScaI AGT'ACT Cuts at: 0 725 2595 Size: 725 1870 SexAI A'CCwGG_T Cuts at: 0 1577 2595 Size: 1577 1018 SfcI C'TryA_G Cuts at: 0 1415 2595 Size: 1415 1180 SmlI C'TyrA_G Cuts at: 0 1062 1127 1756 1848 2212 2565 2595 Size: 1062 65 629 92 364 353 30 Fragments arranged by size: 1062 629 364 353 92 65 30 SpeI A'CTAG_T Cuts at: 0 16 2595 Size: 16 2579 StuI AGG'CCT Cuts at: 0 1171 1285 2595 Size: 1171 114 1310 Fragments arranged by size: 1310 1171 114 StyI C'CwwG_G Cuts at: 0 2355 2595 Size: 2355 240 SwaI ATTT'AAAT Cuts at: 0 852 2595 Size: 852 1743 TatI w'GTAC_w Cuts at: 0 21 723 945 2595 Size: 21 702 222 1650 Fragments arranged by size: 1650 702 222 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2286 2318 2595 Size: 2286 32 277 Fragments arranged by size: 2286 277 32 XbaI T'CTAG_A Cuts at: 0 1995 2089 2329 2595 Size: 1995 94 240 266 Fragments arranged by size: 1995 266 240 94 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1389 2595 Size: 1389 1206 Enzymes that do cut: AccI AcuI AflII AlwNI ApaLI ApoI AseI BanII BbsI BciVI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaBI BsaWI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BstYI Bsu36I DraI DraIII EarI EcoICRI Eco57MI EcoRI Hin4I HincII HpaI MmeI MslI MspA1I NdeI PasI PvuII SacI SapI ScaI SexAI SfcI SmlI SpeI StuI StyI SwaI TatI TstI XbaI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI AloI ApaI AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BclI BfrBI BmgBI BmtI Bpu10I BsaI BsaHI BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI DrdI EaeI EagI EciI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HindIII KasI KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI TaqII Tth111I XhoI XmaI XmnI ZraI