Restriction map for
pFN21AE2677
(Circular) (Six-base) MAPSORT of: pFN21AE2677.seq Check: 6207 from: 1 to: 10165
pFN21AE2677 10165 bp
With 182 enzymes: SgfI *
May 15, 2018 21:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7975 278
Size: 53 83 186 936 6439 2468
Fragments arranged by size:
6439 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2567 7508 1937
Size: 630 4941 4594
Fragments arranged by size:
4941 4594 630
Acc65I G'GTAC_C
Cuts at: 7486 7486
Size: 10165
AclI AA'CG_TT
Cuts at: 1563 8292 8665 1563
Size: 6729 373 3063
Fragments arranged by size:
6729 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2064 3651 4023 4505 4599 5612 5822 8228
Size: 432 1587 372 482 94 1013 210 2406
Cuts at: 8228 9612 9880 1632
Size: 1384 268 1917
Fragments arranged by size:
2406 1917 1587 1384 1013 482 432 372
268 210 94
AfeI AGC'GCT
Cuts at: 5448 5448
Size: 10165
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9968
AflIII A'CryG_T
Cuts at: 1203 4846 9064 10041 1203
Size: 3643 4218 977 1327
Fragments arranged by size:
4218 3643 1327 977
AgeI A'CCGG_T
Cuts at: 8996 8996
Size: 10165
AhdI GACnn_n'nnGTC
Cuts at: 8894 8894
Size: 10165
AleI CACnn'nnGTG
Cuts at: 1166 3015 6311 6938 1166
Size: 1849 3296 627 4393
Fragments arranged by size:
4393 3296 1849 627
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10165
AlwNI CAG_nnn'CTG
Cuts at: 5374 5824 6734 7908 9480 5374
Size: 450 910 1174 1572 6059
Fragments arranged by size:
6059 1572 1174 910 450
ApaLI G'TGCA_C
Cuts at: 8222 9378 8222
Size: 1156 9009
ApoI r'AATT_y
Cuts at: 1493 2089 2123 2339 2384 2807 2835 2882 3351
Size: 596 34 216 45 423 28 47 469
Cuts at: 3351 3460 3797 3804 4349 4780 4809 5133 5155
Size: 109 337 7 545 431 29 324 22
Cuts at: 5155 5602 5612 6774 7046 7069 7199 7356 7474
Size: 447 10 1162 272 23 130 157 118
Cuts at: 7474 7700 7852 9961 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1162 596 545 469 447 431
423 337 324 272 226 216 157 152
130 118 109 47 45 34 29 28
23 22 10 7
AseI AT'TA_AT
Cuts at: 160 1032 4196 4955 5964 8719 160
Size: 872 3164 759 1009 2755 1606
Fragments arranged by size:
3164 2755 1606 1009 872 759
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10165
AvaI C'yCGr_G
Cuts at: 1870 1943 3957 7490 1870
Size: 73 2014 3533 4545
Fragments arranged by size:
4545 3533 2014 73
BamHI G'GATC_C
Cuts at: 7495 7862 7495
Size: 367 9798
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 7486 8941 618
Size: 325 217 627 5699 1455 1842
Fragments arranged by size:
5699 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3847 7274 7484 1391
Size: 2456 3427 210 4072
Fragments arranged by size:
4072 3427 2456 210
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2183 2543 3176 3603 3753 4587 4635
Size: 971 284 360 633 427 150 834 48
Cuts at: 4635 6135 928
Size: 1500 4958
Fragments arranged by size:
4958 1500 971 834 633 427 360 284
150 48
BbvCI CC'TCA_GC
Cuts at: 6468 6468
Size: 10165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8356 8390 1903
Size: 34 6419 34 3678
Fragments arranged by size:
6419 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8057 9267 8057
Size: 1210 8955
BclI T'GATC_A
Cuts at: 1548 4324 6841 7420 1548
Size: 2776 2517 579 4293
Fragments arranged by size:
4293 2776 2517 579
BfrBI ATG'CAT
Cuts at: 2292 2292
Size: 10165
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8776 136
Size: 107 122 71 631 605 7104 1525
Fragments arranged by size:
7104 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2944 5833 6100 10160 2944
Size: 2889 267 4060 2949
Fragments arranged by size:
4060 2949 2889 267
BlpI GC'TnA_GC
Cuts at: 7582 7582
Size: 10165
Bme1580I G_kGCm'C
Cuts at: 1790 8226 9382 1790
Size: 6436 1156 2573
Fragments arranged by size:
6436 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10165
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3213 7896 8854
Size: 416 53 83 399 24 1796 4683 958
Cuts at: 8854 442
Size: 1753
Fragments arranged by size:
4683 1796 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2649 1056
Size: 1593 8572
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5812 5812
Size: 10165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 5993 8825 1239
Size: 926 3828 2832 2579
Fragments arranged by size:
3828 2832 2579 926
Bpu10I CC'TnA_GC
Cuts at: 2320 6468 2320
Size: 4148 6017
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2249 3453 3486 4280 5657 6020 6209 6402 8290
Size: 1204 33 794 1377 363 189 193 1888
Cuts at: 8290 9155 9453 2249
Size: 865 298 2961
Fragments arranged by size:
2961 1888 1377 1204 865 794 363 298
193 189 33
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 8828 882
Size: 617 7329 2219
Fragments arranged by size:
7329 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4982 7956 493
Size: 711 3778 2974 2702
Fragments arranged by size:
3778 2974 2702 711
BsaBI GATnn'nnATC
Cuts at: 6410 10159 6410
Size: 3749 6416
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7972 8354 8989 275
Size: 53 83 186 936 6439 382 635 1451
Fragments arranged by size:
6439 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 8598 8996 9270 9417 9828 8598
Size: 398 274 147 411 8935
Fragments arranged by size:
8935 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 9940
Fragments arranged by size:
9940 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3629 3731 5069 7004 1186
Size: 2443 102 1338 1935 4347
Fragments arranged by size:
4347 2443 1935 1338 102
BseYI C'CCAG_C
Cuts at: 2365 6522 9368 9864 2365
Size: 4157 2846 496 2666
Fragments arranged by size:
4157 2846 2666 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4871 1163
Size: 573 3135 6457
Fragments arranged by size:
6457 3135 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4610 7615 8376 9404 1540
Size: 275 186 2609 3005 761 1028 2301
Fragments arranged by size:
3005 2609 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 7274 8226 8311 9382 7274
Size: 952 85 1071 8057
Fragments arranged by size:
8057 1071 952 85
BsmI GAATG_Cn'
Cuts at: 2514 7027 7676 7769 2514
Size: 4513 649 93 4910
Fragments arranged by size:
4910 4513 649 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3847 7274 7484 8226 8311 9382 1391
Size: 399 2057 3427 210 742 85 1071 2174
Fragments arranged by size:
3427 2174 2057 1071 742 399 210 85
BspEI T'CCGG_A
Cuts at: 9828 9828
Size: 10165
BspHI T'CATG_A
Cuts at: 2119 4887 8054 9784 2119
Size: 2768 3167 1730 2500
Fragments arranged by size:
3167 2768 2500 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 7520 844
Size: 6676 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 8052 1394
Size: 429 6229 3507
Fragments arranged by size:
6229 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2657 3426 4399 4614 6259 8660 8834
Size: 1183 1409 769 973 215 1645 2401 174
Cuts at: 8834 65
Size: 1396
Fragments arranged by size:
2401 1645 1409 1396 1183 973 769 215
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8809 8996 1700
Size: 110 6999 187 2869
Fragments arranged by size:
6999 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 6625 96
Size: 6529 3636
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10165
BssSI C'ACGA_G
Cuts at: 3108 3713 8225 9237 3108
Size: 605 4512 1012 4036
Fragments arranged by size:
4512 4036 1012 605
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5807 794
Size: 5013 5152
BstBI TT'CG_AA
Cuts at: 2163 2194 7447 8969 2163
Size: 31 5253 1522 3359
Fragments arranged by size:
5253 3359 1522 31
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10165
BstXI CCAn_nnnn'nTGG
Cuts at: 6229 6229
Size: 10165
BstYI r'GATC_y
Cuts at: 1972 2594 2944 5035 5833 6100 6730 6800 7495
Size: 622 350 2091 798 267 630 70 695
Cuts at: 7495 7862 8247 8264 9716 9802 9814 10160 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2091 1977 1452 798 695 630 622 385
367 350 346 267 86 70 17 12
BstZ17I GTA'TAC
Cuts at: 2568 2568
Size: 10165
Bsu36I CC'TnA_GG
Cuts at: 4464 4464
Size: 10165
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 8674
Fragments arranged by size:
8674 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9426
Fragments arranged by size:
9426 683 56
BtsI GCAGTG_nn'
Cuts at: 6918 7681 8475 8495 6918
Size: 763 794 20 8588
Fragments arranged by size:
8588 794 763 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10165
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3976 4011 529
Size: 35 3412 35 6683
Fragments arranged by size:
6683 3412 35 35
DraI TTT'AAA
Cuts at: 2994 3459 3802 4387 5658 7469 2994
Size: 465 343 585 1271 1811 5690
Fragments arranged by size:
5690 1811 1271 585 465 343
DraIII CAC_nnn'GTG
Cuts at: 5468 5468
Size: 10165
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2439 9172 809
Size: 507 1123 6733 1802
Fragments arranged by size:
6733 1802 1123 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6219 7167 7612
Size: 54 1425 50 275 18 4389 948 445
Cuts at: 7612 8501 10130 8
Size: 889 1629 43
Fragments arranged by size:
4389 1629 1425 948 889 445 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7612 1537
Size: 275 5800 4090
Fragments arranged by size:
5800 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4053 4116 4206 5221 6962 6968 7279 8095
Size: 2700 63 90 1015 1741 6 311 816
Cuts at: 8095 1353
Size: 3423
Fragments arranged by size:
3423 2700 1741 1015 816 311 90 63
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3412 8752 9126 9272 387
Size: 1064 138 1823 5340 374 146 1280
Fragments arranged by size:
5340 1823 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 7272 7272
Size: 10165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2165 3651 4023 4505 4599 5612
Size: 393 432 101 1486 372 482 94 1013
Cuts at: 5612 5822 5993 8228 8825 9612 9880 1239
Size: 210 171 2235 597 787 268 1524
Fragments arranged by size:
2235 1524 1486 1013 787 597 482 432
393 372 268 210 171 101 94
EcoRI G'AATT_C
Cuts at: 2384 5133 7474 7852 2384
Size: 2749 2341 378 4697
Fragments arranged by size:
4697 2749 2341 378
EcoRV GAT'ATC
Cuts at: 6615 6703 6615
Size: 88 10077
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2404 5378 5642 5852 2404
Size: 2974 264 210 6717
Fragments arranged by size:
6717 2974 264 210
FspI TGC'GCA
Cuts at: 8671 8671
Size: 10165
HaeII r_GCGC'y
Cuts at: 1678 5450 9312 1678
Size: 3772 3862 2531
Fragments arranged by size:
3862 3772 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4182 4214 4460 4492 6785
Size: 32 135 32 2610 32 246 32 2293
Cuts at: 6785 6792 6817 6824 8806 8838 8880 8912 1373
Size: 7 25 7 1982 32 42 32 2626
Fragments arranged by size:
2626 2610 2293 1982 246 135 42 32
32 32 32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 5700 6831 7509 7755 669
Size: 988 281 3762 1131 678 246 3079
Fragments arranged by size:
3762 3079 1131 988 678 281 246
HindIII A'AGCT_T
Cuts at: 748 2105 748
Size: 1357 8808
HpaI GTT'AAC
Cuts at: 5700 7755 5700
Size: 2055 8110
KpnI G_GTAC'C
Cuts at: 7490 7490
Size: 10165
MfeI C'AATT_G
Cuts at: 2067 4833 7764 2067
Size: 2766 2931 4468
Fragments arranged by size:
4468 2931 2766
MluI A'CGCG_T
Cuts at: 10041 10041
Size: 10165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3714 4828 9279 9463 1305
Size: 2409 1114 4451 184 2007
Fragments arranged by size:
4451 2409 2007 1114 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6221 7169 10
Size: 54 1425 343 4389 948 3006
Fragments arranged by size:
4389 3006 1425 948 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3015 4017 4387 6137 6311 6938 8123
Size: 648 1849 1002 370 1750 174 627 1185
Cuts at: 8123 8482 8641 518
Size: 359 159 2042
Fragments arranged by size:
2042 1849 1750 1185 1002 648 627 370
359 174 159
MspA1I CmG'CkG
Cuts at: 1606 5374 6526 7581 8258 9406 9651 1606
Size: 3768 1152 1055 677 1148 245 2120
Fragments arranged by size:
3768 2120 1152 1148 1055 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 8674
Fragments arranged by size:
8674 939 552
NdeI CA'TA_TG
Cuts at: 387 4371 387
Size: 3984 6181
NheI G'CTAG_C
Cuts at: 1052 2645 1052
Size: 1593 8572
NotI GC'GGCC_GC
Cuts at: 7612 7612
Size: 10165
NsiI A_TGCA'T
Cuts at: 2294 2294
Size: 10165
NspI r_CATG'y
Cuts at: 1132 1229 3290 4850 5429 6096 6136 7523 9068
Size: 97 2061 1560 579 667 40 1387 1545
Cuts at: 9068 1132
Size: 2229
Fragments arranged by size:
2229 2061 1560 1545 1387 667 579 97
40
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10165
PciI A'CATG_T
Cuts at: 4846 9064 4846
Size: 4218 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3567 1108
Size: 2459 7706
PfoI T'CCnGG_A
Cuts at: 3297 3297
Size: 10165
PmeI GTTT'AAAC
Cuts at: 7469 7469
Size: 10165
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8234 8266 9747 9779 8234
Size: 32 1481 32 8620
Fragments arranged by size:
8620 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10165
PsiI TTA'TAA
Cuts at: 2939 7735 2939
Size: 4796 5369
PstI C_TGCA'G
Cuts at: 830 7517 830
Size: 6687 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10165
PvuII CAG'CTG
Cuts at: 5374 6526 5374
Size: 1152 9013
SacI G_AGCT'C
Cuts at: 7274 7274
Size: 10165
SalI G'TCGA_C
Cuts at: 1936 7507 1936
Size: 5571 4594
SapI GCTCTTCn'nnn_
Cuts at: 7279 7279
Size: 10165
SbfI CC_TGCA'GG
Cuts at: 7517 7517
Size: 10165
ScaI AGT'ACT
Cuts at: 8413 10125 8413
Size: 1712 8453
SfcI C'TryA_G
Cuts at: 826 1045 2983 6804 7193 7513 8648 9329 9520
Size: 219 1938 3821 389 320 1135 681 191
Cuts at: 9520 826
Size: 1471
Fragments arranged by size:
3821 1938 1471 1135 681 389 320 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10165
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10165
SmaI CCC'GGG
Cuts at: 1872 7492 1872
Size: 5620 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2228 3468 3501 3957 4259 5672
Size: 197 926 285 1240 33 456 302 1413
Cuts at: 5672 6035 6188 6417 8269 9170 9432 820
Size: 363 153 229 1852 901 262 1553
Fragments arranged by size:
1852 1553 1413 1240 926 901 456 363
302 285 262 229 197 153 33
SnaBI TAC'GTA
Cuts at: 493 4982 7956 493
Size: 4489 2974 2702
Fragments arranged by size:
4489 2974 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10165
SphI G_CATG'C
Cuts at: 1132 6096 7523 1132
Size: 4964 1427 3774
Fragments arranged by size:
4964 3774 1427
SspI AAT'ATT
Cuts at: 5 52 2509 3081 3540 8089 5
Size: 47 2457 572 459 4549 2081
Fragments arranged by size:
4549 2457 2081 572 459 47
StuI AGG'CCT
Cuts at: 1517 2077 5213 1517
Size: 560 3136 6469
Fragments arranged by size:
6469 3136 560
StyI C'CwwG_G
Cuts at: 513 1065 2004 2296 2352 3792 6957 7604 513
Size: 552 939 292 56 1440 3165 647 3074
Fragments arranged by size:
3165 3074 1440 939 647 552 292 56
SwaI ATTT'AAAT
Cuts at: 4387 5658 4387
Size: 1271 8894
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4624 7045 8192 8209 8362 8547 8992
Size: 1255 3044 2421 1147 17 153 185 445
Cuts at: 8992 325
Size: 1498
Fragments arranged by size:
3044 2421 1498 1255 1147 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5908 6625 8411
Size: 274 80 33 51 1443 3931 717 1786
Cuts at: 8411 10123 96
Size: 1712 138
Fragments arranged by size:
3931 1786 1712 1443 717 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6841 6873 10125 10157 6841
Size: 32 3252 32 6849
Fragments arranged by size:
6849 3252 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2436 1316
Size: 1120 9045
XbaI T'CTAG_A
Cuts at: 3580 7501 3580
Size: 3921 6244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2788 2788
Size: 10165
XhoI C'TCGA_G
Cuts at: 1943 3957 1943
Size: 2014 8151
XmaI C'CCGG_G
Cuts at: 1870 7490 1870
Size: 5620 4545
XmnI GAAnn'nnTTC
Cuts at: 2384 2388 5625 7072 8294 2384
Size: 4 3237 1447 1222 4255
Fragments arranged by size:
4255 3237 1447 1222 4
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7973 276
Size: 53 83 186 936 6439 2468
Fragments arranged by size:
6439 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI