################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AatII AflII Alw44I AocI ApaLI AscI AseI AsnI AspI AviII AvrII BfrI BlnI Bsp1407I BspDI BspLU11I BsrGI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI Eco81I EcoT22I FspI HpaI MfeI MunI NotI NruI NsiI NspI NspV PacI PflFI PmeI PshBI PvuI RsrII SbfI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 5443 GGTACC G^GTACC AgeI 2662 ACCGGT A^CCGGT Aor51HI 262 AGCGCT AGC^GCT Asp718I 5443 GGTACC G^GTACC BbeI 14 GGCGCC GGCGC^C BglII 3275 AGATCT A^GATCT BsiWI 5564 CGTACG C^GTACG BspHI 4113 TCATGA T^CATGA BssHII 4611 GCGCGC G^CGCGC DraI 2109 TTTAAA TTT^AAA DrdI 4249 GACTCTACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 262 AGCGCT AGC^GCT EcoRI 3824 GAATTC G^AATTC EcoRV 2842 GATATC GAT^ATC FseI 167 GGCCGGCC GGCCGG^CC KasI 14 GGCGCC G^GCGCC KpnI 5443 GGTACC GGTAC^C KspI 4623 CCGCGG CCGC^GG MluI 2522 ACGCGT A^CGCGT NarI 14 GGCGCC GG^CGCC NheI 4432 GCTAGC G^CTAGC PaeR7I 2179 CTCGAG C^TCGAG PinAI 2662 ACCGGT A^CCGGT PshAI 2370 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 4113 TCATGA T^CATGA SacII 4623 CCGCGG CCGC^GG SfoI 14 GGCGCC GGC^GCC SgrAI 2661 CACCGGTG C[GA]^CCGG[CT]G XhoI 2179 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccIII 198 TCCGGA T^CCGGA 3873 TCCGGA AclI 1820 AACGTT AA^CGTT 4787 AACGTT AhdI 1463 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4651 GACGACCTGTC AspEI 1463 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4651 GACGACCTGTC BamHI 2539 GGATCC G^GATCC 4932 GGATCC BbrPI 2461 CACGTG CAC^GTG 4402 CACGTG BcgI 5076 CGACGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1666 GCATTTTTCTCG BclI 2513 TGATCA T^GATCA 2867 TGATCA BsaI 1751 GAGACC GGTCTC(1/5) 918 GGTCTC BseAI 198 TCCGGA T^CCGGA 3873 TCCGGA BsmI 1057 GCATTC GAATGC(1/-1) 1688 GCATTC BspEI 198 TCCGGA T^CCGGA 3873 TCCGGA BsrBI 81 GAGCGG CCGCTC(-3/-3) 4341 GAGCGG BstAPI 1048 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2293 GCAGTCACTGC BstEII 3817 GGTTACC G^GT[ACGT]ACC 5677 GGTTACC BstPI 3817 GGTTACC G^GT[ACGT]ACC 5677 GGTTACC Eam1105I 1463 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4651 GACGACCTGTC EcoO65I 3817 GGTTACC G^GT[ACGT]ACC 5677 GGTTACC FbaI 2513 TGATCA T^GATCA 2867 TGATCA HindIII 2160 AAGCTT A^AGCTT 3061 AAGCTT MroI 198 TCCGGA T^CCGGA 3873 TCCGGA NdeI 662 CATATG CA^TATG 2831 CATATG PmaCI 2461 CACGTG CAC^GTG 4402 CACGTG PmlI 2461 CACGTG CAC^GTG 4402 CACGTG Psp1406I 1820 AACGTT AA^CGTT 4787 AACGTT PvuII 2044 CAGCTG CAG^CTG 3592 CAGCTG SalI 2797 GTCGAC G^TCGAC 3850 GTCGAC SapI 2888 GAAGAGC GCTCTTC(1/4) 4770 GAAGAGC ScaI 2928 AGTACT AGT^ACT 3144 AGTACT SexAI 4814 ACCAGGT A^CC[AT]GGT 5295 ACCAGGT SfiI 162 GGCCCGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 5549 GGCCAGGCTGGCC SmaI 72 CCCGGG CCC^GGG 123 CCCGGG StuI 3210 AGGCCT AGG^CCT 5360 AGGCCT XbaI 1483 TCTAGA T^CTAGA 2451 TCTAGA XmaCI 72 CCCGGG C^CCGGG 123 CCCGGG XmaI 72 CCCGGG C^CCGGG 123 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 143 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI