(Circular) (Six-base) MAPSORT of: pFN21AE2750.seq Check: 9321 from: 1 to: 10507 pFN21AE2750 10507 bp With 182 enzymes: SgfI * June 21, 2019 12:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3946 5662 7118 3946 Size: 1716 1456 7335 Fragments arranged by size: 7335 1716 1456 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8317 278 Size: 53 83 186 936 6781 2468 Fragments arranged by size: 6781 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4202 4803 5856 6654 7328 7850 1937 Size: 2265 601 1053 798 674 522 4594 Fragments arranged by size: 4594 2265 1053 798 674 601 522 Acc65I G'GTAC_C Cuts at: 7448 7828 7448 Size: 380 10127 AclI AA'CG_TT Cuts at: 1563 3826 6793 8634 9007 1563 Size: 2263 2967 1841 373 3063 Fragments arranged by size: 3063 2967 2263 1841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 3777 4581 4988 5138 5522 6167 7599 Size: 635 1510 804 407 150 384 645 1432 Cuts at: 7599 8570 9954 10222 1632 Size: 971 1384 268 1917 Fragments arranged by size: 1917 1510 1432 1384 971 804 645 635 407 384 268 150 AfeI AGC'GCT Cuts at: 2269 2269 Size: 10507 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10310 AflIII A'CryG_T Cuts at: 1203 2430 4467 4527 9406 10383 1203 Size: 1227 2037 60 4879 977 1327 Fragments arranged by size: 4879 2037 1327 1227 977 60 AgeI A'CCGG_T Cuts at: 4667 9338 4667 Size: 4671 5836 AhdI GACnn_n'nnGTC Cuts at: 3473 6661 9236 3473 Size: 3188 2575 4744 Fragments arranged by size: 4744 3188 2575 AleI CACnn'nnGTG Cuts at: 1166 2975 4625 4649 4880 1166 Size: 1809 1650 24 231 6793 Fragments arranged by size: 6793 1809 1650 231 24 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2286 2816 3383 1184 Size: 1102 530 567 8308 Fragments arranged by size: 8308 1102 567 530 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6778 6810 6778 Size: 32 10475 AlwNI CAG_nnn'CTG Cuts at: 2294 4304 5500 6037 6082 6251 7474 8250 9822 Size: 2010 1196 537 45 169 1223 776 1572 Cuts at: 9822 2294 Size: 2979 Fragments arranged by size: 2979 2010 1572 1223 1196 776 537 169 45 ApaI G_GGCC'C Cuts at: 4607 5330 7673 4607 Size: 723 2343 7441 Fragments arranged by size: 7441 2343 723 ApaLI G'TGCA_C Cuts at: 8564 9720 8564 Size: 1156 9351 ApoI r'AATT_y Cuts at: 1493 2749 3422 5829 7816 8042 8194 10303 1493 Size: 1256 673 2407 1987 226 152 2109 1697 Fragments arranged by size: 2407 2109 1987 1697 1256 673 226 152 AseI AT'TA_AT Cuts at: 160 1032 9061 160 Size: 872 8029 1606 Fragments arranged by size: 8029 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10507 AvaI C'yCGr_G Cuts at: 1870 1943 2077 2128 3760 4184 6667 7832 1870 Size: 73 134 51 1632 424 2483 1165 4545 Fragments arranged by size: 4545 2483 1632 1165 424 134 73 51 BamHI G'GATC_C Cuts at: 4544 6937 7837 8204 4544 Size: 2393 900 367 6847 Fragments arranged by size: 6847 2393 900 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2019 3344 4312 4627 6490 Size: 325 217 627 232 1325 968 315 1863 Cuts at: 6490 6881 7339 7448 7828 9283 618 Size: 391 458 109 380 1455 1842 Fragments arranged by size: 1863 1842 1455 1325 968 627 458 391 380 325 315 232 217 109 BanII G_rGCy'C Cuts at: 1391 2199 2241 3236 4607 4730 5330 5466 6340 Size: 808 42 995 1371 123 600 136 874 Cuts at: 6340 6771 6782 7510 7629 7673 7761 7826 1391 Size: 431 11 728 119 44 88 65 4072 Fragments arranged by size: 4072 1371 995 874 808 728 600 431 136 123 119 88 65 44 42 11 BbeI G_GCGC'C Cuts at: 2023 2023 Size: 10507 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3795 4266 4401 5763 7266 7710 928 Size: 971 1896 471 135 1362 1503 444 3725 Fragments arranged by size: 3725 1896 1503 1362 971 471 444 135 BbvCI CC'TCA_GC Cuts at: 3291 4240 7353 7630 3291 Size: 949 3113 277 6168 Fragments arranged by size: 6168 3113 949 277 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3660 3694 7070 7104 8698 Size: 34 50 34 1639 34 3376 34 1594 Cuts at: 8698 8732 1903 Size: 34 3678 Fragments arranged by size: 3678 3376 1639 1594 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5170 5432 5484 7002 8399 9609 5170 Size: 262 52 1518 1397 1210 6068 Fragments arranged by size: 6068 1518 1397 1210 262 52 BclI T'GATC_A Cuts at: 1548 4518 4872 1548 Size: 2970 354 7183 Fragments arranged by size: 7183 2970 354 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2174 7097 7232 Size: 107 122 71 631 605 502 4923 135 Cuts at: 7232 7561 9118 136 Size: 329 1557 1525 Fragments arranged by size: 4923 1557 1525 631 605 502 329 135 122 107 71 BglII A'GATC_T Cuts at: 5280 10502 5280 Size: 5222 5285 BlpI GC'TnA_GC Cuts at: 5603 6072 7509 7924 5603 Size: 469 1437 415 8186 Fragments arranged by size: 8186 1437 469 415 Bme1580I G_kGCm'C Cuts at: 1790 2265 4317 4607 5330 6636 6709 6753 7344 Size: 475 2052 290 723 1306 73 44 591 Cuts at: 7344 7673 8568 9724 1790 Size: 329 895 1156 2573 Fragments arranged by size: 2573 2052 1306 1156 895 723 591 475 329 290 73 44 BmgBI CAC'GTC Cuts at: 1318 3328 6181 1318 Size: 2010 2853 5644 Fragments arranged by size: 5644 2853 2010 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2268 4056 5438 Size: 416 53 83 399 24 851 1788 1382 Cuts at: 5438 5921 6357 6447 6992 7012 7195 8238 9196 Size: 483 436 90 545 20 183 1043 958 Cuts at: 9196 442 Size: 1753 Fragments arranged by size: 1788 1753 1382 1043 958 851 545 483 436 416 399 183 90 83 53 24 20 BmtI G_CTAG'C Cuts at: 1056 6441 1056 Size: 5385 5122 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5771 5892 6400 5771 Size: 121 508 9878 Fragments arranged by size: 9878 508 121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2919 3148 3448 4076 5537 5714 5783 7694 Size: 1680 229 300 628 1461 177 69 1911 Cuts at: 7694 9167 1239 Size: 1473 2579 Fragments arranged by size: 2579 1911 1680 1473 1461 628 300 229 177 69 Bpu10I CC'TnA_GC Cuts at: 3230 3291 4240 7353 7630 3230 Size: 61 949 3113 277 6107 Fragments arranged by size: 6107 3113 949 277 61 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3423 4097 4289 5290 5428 8632 9497 9795 3423 Size: 674 192 1001 138 3204 865 298 4135 Fragments arranged by size: 4135 3204 1001 865 674 298 192 138 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2929 3750 9170 882 Size: 617 1430 821 5420 2219 Fragments arranged by size: 5420 2219 1430 821 617 BsaAI yAC'GTr Cuts at: 493 1204 2431 4468 6409 8298 493 Size: 711 1227 2037 1941 1889 2702 Fragments arranged by size: 2702 2037 1941 1889 1227 711 BsaBI GATnn'nnATC Cuts at: 4866 5706 6716 6931 10501 4866 Size: 840 1010 215 3570 4872 Fragments arranged by size: 4872 3570 1010 840 215 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2020 2026 3599 3650 Size: 53 83 186 936 487 6 1573 51 Cuts at: 3650 4713 5535 6302 6903 7083 8314 8696 9331 Size: 1063 822 767 601 180 1231 382 635 Cuts at: 9331 275 Size: 1451 Fragments arranged by size: 1573 1451 1231 1063 936 822 767 635 601 487 382 186 180 83 53 51 6 BsaWI w'CCGG_w Cuts at: 2203 4667 5012 5710 5878 8940 9338 9612 9759 Size: 2464 345 698 168 3062 398 274 147 Cuts at: 9759 10170 2203 Size: 411 2540 Fragments arranged by size: 3062 2540 2464 698 411 398 345 274 168 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3001 3031 3064 3094 6404 Size: 30 165 30 1598 30 33 30 3310 Cuts at: 6404 6434 7403 7433 1178 Size: 30 969 30 4252 Fragments arranged by size: 4252 3310 1598 969 165 33 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2954 3087 4740 5307 7061 7424 7714 7751 Size: 1768 133 1653 567 1754 363 290 37 Cuts at: 7751 7769 1186 Size: 18 3924 Fragments arranged by size: 3924 1768 1754 1653 567 363 290 133 37 18 BseYI C'CCAG_C Cuts at: 2307 2408 5199 5595 5847 6514 6949 7032 7103 Size: 101 2791 396 252 667 435 83 71 Cuts at: 7103 7222 7678 9710 10206 2307 Size: 119 456 2032 496 2608 Fragments arranged by size: 2791 2608 2032 667 496 456 435 396 252 119 101 83 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2256 2979 3259 4290 4922 4977 5297 Size: 573 520 723 280 1031 632 55 320 Cuts at: 5297 5690 5781 6980 7811 1163 Size: 393 91 1199 831 3859 Fragments arranged by size: 3859 1199 1031 831 723 632 573 520 393 320 280 91 55 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2092 2174 3583 6523 7957 8718 Size: 275 186 91 82 1409 2940 1434 761 Cuts at: 8718 9746 1540 Size: 1028 2301 Fragments arranged by size: 2940 2301 1434 1409 1028 761 275 186 91 82 BsiHKAI G_wGCw'C Cuts at: 2199 2241 4456 4658 6340 6408 6594 6782 7510 Size: 42 2215 202 1682 68 186 188 728 Cuts at: 7510 7761 7787 8568 8653 9724 2199 Size: 251 26 781 85 1071 2982 Fragments arranged by size: 2982 2215 1682 1071 781 728 251 202 188 186 85 68 42 26 BsiWI C'GTAC_G Cuts at: 7569 7569 Size: 10507 BsmI GAATG_Cn' Cuts at: 3062 3693 8018 8111 3062 Size: 631 4325 93 5458 Fragments arranged by size: 5458 4325 631 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3503 3948 5880 6171 3503 Size: 445 1932 291 7839 Fragments arranged by size: 7839 1932 445 291 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2199 2241 2265 3236 4317 4456 4607 Size: 399 409 42 24 971 1081 139 151 Cuts at: 4607 4658 4730 5330 5466 6340 6408 6594 6636 Size: 51 72 600 136 874 68 186 42 Cuts at: 6636 6709 6753 6771 6782 7344 7510 7629 7673 Size: 73 44 18 11 562 166 119 44 Cuts at: 7673 7761 7787 7826 8568 8653 9724 1391 Size: 88 26 39 742 85 1071 2174 Fragments arranged by size: 2174 1081 1071 971 874 742 600 562 409 399 186 166 151 139 136 119 88 85 73 72 68 51 44 44 42 42 39 26 24 18 11 BspEI T'CCGG_A Cuts at: 2203 5878 10170 2203 Size: 3675 4292 2540 Fragments arranged by size: 4292 3675 2540 BspHI T'CATG_A Cuts at: 6118 8396 10126 6118 Size: 2278 1730 6499 Fragments arranged by size: 6499 2278 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3946 4542 5662 6315 7118 7862 844 Size: 3102 596 1120 653 803 744 3489 Fragments arranged by size: 3489 3102 1120 803 744 653 596 BsrBI CCG'CTC Cuts at: 1394 1823 2088 6348 8394 1394 Size: 429 265 4260 2046 3507 Fragments arranged by size: 4260 3507 2046 429 265 BsrDI GCAATG_nn' Cuts at: 65 1248 3083 5841 7289 9002 9176 65 Size: 1183 1835 2758 1448 1713 174 1396 Fragments arranged by size: 2758 1835 1713 1448 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2173 3595 4667 6441 6855 9151 9338 Size: 110 363 1422 1072 1774 414 2296 187 Cuts at: 9338 1700 Size: 2869 Fragments arranged by size: 2869 2296 1774 1422 1072 414 363 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10507 BssHII G'CGCG_C Cuts at: 1925 6616 1925 Size: 4691 5816 BssSI C'ACGA_G Cuts at: 2179 2240 3307 7094 8567 9579 2179 Size: 61 1067 3787 1473 1012 3107 Fragments arranged by size: 3787 3107 1473 1067 1012 61 BstAPI GCAn_nnn'nTGC Cuts at: 794 3059 4304 794 Size: 2265 1245 6997 Fragments arranged by size: 6997 2265 1245 BstBI TT'CG_AA Cuts at: 9311 9311 Size: 10507 BstEII G'GTnAC_C Cuts at: 1184 5822 7682 1184 Size: 4638 1860 4009 Fragments arranged by size: 4638 4009 1860 BstXI CCAn_nnnn'nTGG Cuts at: 3261 4705 5818 3261 Size: 1444 1113 7950 Fragments arranged by size: 7950 1444 1113 BstYI r'GATC_y Cuts at: 1972 2404 3433 3491 4544 5280 6042 6201 6417 Size: 432 1029 58 1053 736 762 159 216 Cuts at: 6417 6459 6606 6726 6937 7398 7464 7837 8204 Size: 42 147 120 211 461 66 373 367 Cuts at: 8204 8589 8606 10058 10144 10156 10502 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1053 1029 762 736 461 432 385 373 367 346 216 211 159 147 120 86 66 58 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2040 2232 2510 2601 3495 3577 4157 Size: 552 975 192 278 91 894 82 580 Cuts at: 4157 4644 6628 6738 7698 513 Size: 487 1984 110 960 3322 Fragments arranged by size: 3322 1984 975 960 894 580 552 487 278 192 110 91 82 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3030 6581 6891 487 Size: 683 56 1804 3551 310 4103 Fragments arranged by size: 4103 3551 1804 683 310 56 BtsI GCAGTG_nn' Cuts at: 3666 4302 4965 6131 6356 8023 8817 8837 3666 Size: 636 663 1166 225 1667 794 20 5336 Fragments arranged by size: 5336 1667 1166 794 663 636 225 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10507 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2338 2373 4057 4092 529 Size: 35 1774 35 1684 35 6944 Fragments arranged by size: 6944 1774 1684 35 35 35 DraI TTT'AAA Cuts at: 4116 7811 4116 Size: 3695 6812 DraIII CAC_nnn'GTG Cuts at: 3264 6694 7203 3264 Size: 3430 509 6568 Fragments arranged by size: 6568 3430 509 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6260 9514 809 Size: 507 4944 3254 1802 Fragments arranged by size: 4944 3254 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2089 2171 Size: 54 1425 50 275 18 207 52 82 Cuts at: 2171 2454 3303 3580 4887 6012 6741 7233 7717 Size: 283 849 277 1307 1125 729 492 484 Cuts at: 7717 7954 8843 10472 8 Size: 237 889 1629 43 Fragments arranged by size: 1629 1425 1307 1125 889 849 729 492 484 283 277 275 237 207 82 54 52 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2089 2171 3580 7954 1537 Size: 275 277 82 1409 4374 4090 Fragments arranged by size: 4374 4090 1409 277 275 82 EarI CTCTTCn'nnn_ Cuts at: 1353 3925 4062 4888 5861 6226 6264 6770 7179 Size: 2572 137 826 973 365 38 506 409 Cuts at: 7179 7588 8437 1353 Size: 409 849 3423 Fragments arranged by size: 3423 2572 973 849 826 506 409 409 365 137 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2092 2668 5641 6545 6599 7360 Size: 1064 138 503 576 2973 904 54 761 Cuts at: 7360 9094 9468 9614 387 Size: 1734 374 146 1280 Fragments arranged by size: 2973 1734 1280 1064 904 761 576 503 374 146 138 54 EcoICRI GAG'CTC Cuts at: 2197 2239 6338 6780 7508 7759 2197 Size: 42 4099 442 728 251 4945 Fragments arranged by size: 4945 4099 728 442 251 42 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2919 3148 3448 3777 4076 4581 Size: 393 635 652 229 300 329 299 505 Cuts at: 4581 4988 5138 5522 5537 5714 5783 6167 7599 Size: 407 150 384 15 177 69 384 1432 Cuts at: 7599 7694 8570 9167 9954 10222 1239 Size: 95 876 597 787 268 1524 Fragments arranged by size: 1524 1432 876 787 652 635 597 505 407 393 384 384 329 300 299 268 229 177 150 95 69 15 EcoNI CCTnn'n_nnAGG Cuts at: 2825 6889 7478 2825 Size: 4064 589 5854 Fragments arranged by size: 5854 4064 589 EcoO109I rG'GnC_Cy Cuts at: 5326 6140 6327 6448 6894 7136 7209 7669 7670 Size: 814 187 121 446 242 73 460 1 Cuts at: 7670 5326 Size: 8163 Fragments arranged by size: 8163 814 460 446 242 187 121 73 1 EcoRI G'AATT_C Cuts at: 5829 7816 8194 5829 Size: 1987 378 8142 Fragments arranged by size: 8142 1987 378 EcoRV GAT'ATC Cuts at: 4849 4849 Size: 10507 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4188 6010 4188 Size: 1822 8685 FseI GG_CCGG'CC Cuts at: 2177 2177 Size: 10507 FspI TGC'GCA Cuts at: 9013 9013 Size: 10507 HaeII r_GCGC'y Cuts at: 1678 2023 2271 6131 6508 6628 9654 1678 Size: 345 248 3860 377 120 3026 2531 Fragments arranged by size: 3860 3026 2531 377 345 248 120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2183 2215 2506 2538 3064 Size: 32 135 32 611 32 291 32 526 Cuts at: 3064 3096 3122 3154 3317 3349 3459 3491 3857 Size: 32 26 32 163 32 110 32 366 Cuts at: 3857 3889 4025 4057 5796 5828 6239 6271 6311 Size: 32 136 32 1739 32 411 32 40 Cuts at: 6311 6343 6404 6436 6647 6650 6679 6682 7403 Size: 32 61 32 211 3 29 3 721 Cuts at: 7403 7435 9148 9180 9222 9254 1373 Size: 32 1713 32 42 32 2626 Fragments arranged by size: 2626 1739 1713 721 611 526 411 366 291 211 163 136 135 110 61 42 40 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 29 26 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 4804 5857 6316 6826 7851 8097 Size: 988 281 2866 1053 459 510 1025 246 Cuts at: 8097 669 Size: 3079 Fragments arranged by size: 3079 2866 1053 1025 988 510 459 281 246 HindIII A'AGCT_T Cuts at: 748 4165 5066 748 Size: 3417 901 6189 Fragments arranged by size: 6189 3417 901 HpaI GTT'AAC Cuts at: 8097 8097 Size: 10507 KasI G'GCGC_C Cuts at: 2019 2019 Size: 10507 KpnI G_GTAC'C Cuts at: 7452 7832 7452 Size: 380 10127 MfeI C'AATT_G Cuts at: 8106 8106 Size: 10507 MluI A'CGCG_T Cuts at: 4527 10383 4527 Size: 5856 4651 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2759 5361 9621 9805 1305 Size: 1454 2602 4260 184 2007 Fragments arranged by size: 4260 2602 2007 1454 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3305 4889 6014 7719 10 Size: 54 1425 343 1473 1584 1125 1705 2798 Fragments arranged by size: 2798 1705 1584 1473 1425 1125 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2417 2975 3365 4124 4625 4649 4703 Size: 648 1251 558 390 759 501 24 54 Cuts at: 4703 4880 5048 5927 8465 8824 8983 518 Size: 177 168 879 2538 359 159 2042 Fragments arranged by size: 2538 2042 1251 879 759 648 558 501 390 359 177 168 159 54 24 MspA1I CmG'CkG Cuts at: 1606 2109 2663 3898 3909 4051 5599 6630 7923 Size: 503 554 1235 11 142 1548 1031 1293 Cuts at: 7923 8600 9748 9993 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1548 1293 1235 1148 1031 677 554 503 245 142 11 NaeI GCC'GGC Cuts at: 2175 3597 6443 2175 Size: 1422 2846 6239 Fragments arranged by size: 6239 2846 1422 NarI GG'CG_CC Cuts at: 2020 2020 Size: 10507 NcoI C'CATG_G Cuts at: 513 1065 2040 2232 2601 3495 4157 513 Size: 552 975 192 369 894 662 6863 Fragments arranged by size: 6863 975 894 662 552 369 192 NdeI CA'TA_TG Cuts at: 387 2668 4837 387 Size: 2281 2169 6057 Fragments arranged by size: 6057 2281 2169 NgoMIV G'CCGG_C Cuts at: 2173 3595 6441 2173 Size: 1422 2846 6239 Fragments arranged by size: 6239 2846 1422 NheI G'CTAG_C Cuts at: 1052 6437 1052 Size: 5385 5122 NotI GC'GGCC_GC Cuts at: 7954 7954 Size: 10507 NspI r_CATG'y Cuts at: 1132 1229 7865 9410 1132 Size: 97 6636 1545 2229 Fragments arranged by size: 6636 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2562 7673 1348 Size: 1214 5111 4182 Fragments arranged by size: 5111 4182 1214 PciI A'CATG_T Cuts at: 9406 9406 Size: 10507 PflMI CCAn_nnn'nTGG Cuts at: 1108 2741 7203 7307 1108 Size: 1633 4462 104 4308 Fragments arranged by size: 4462 4308 1633 104 PfoI T'CCnGG_A Cuts at: 6729 7074 7640 6729 Size: 345 566 9596 Fragments arranged by size: 9596 566 345 PmeI GTTT'AAAC Cuts at: 7811 7811 Size: 10507 PmlI CAC'GTG Cuts at: 4468 6409 4468 Size: 1941 8566 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2568 2600 5117 5149 8576 8608 10089 10121 2568 Size: 32 2517 32 3427 32 1481 32 2954 Fragments arranged by size: 3427 2954 2517 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 6140 6327 6448 7209 6140 Size: 187 121 761 9438 Fragments arranged by size: 9438 761 187 121 PshAI GACnn'nnGTC Cuts at: 1613 4379 1613 Size: 2766 7741 PsiI TTA'TAA Cuts at: 5057 8077 5057 Size: 3020 7487 PspOMI G'GGCC_C Cuts at: 4603 5326 7669 4603 Size: 723 2343 7441 Fragments arranged by size: 7441 2343 723 PstI C_TGCA'G Cuts at: 830 4041 5454 5656 6042 7250 7326 7668 7859 Size: 3211 1413 202 386 1208 76 342 191 Cuts at: 7859 830 Size: 3478 Fragments arranged by size: 3478 3211 1413 1208 386 342 202 191 76 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10507 PvuII CAG'CTG Cuts at: 4051 5599 4051 Size: 1548 8959 SacI G_AGCT'C Cuts at: 2199 2241 6340 6782 7510 7761 2199 Size: 42 4099 442 728 251 4945 Fragments arranged by size: 4945 4099 728 442 251 42 SacII CC_GC'GG Cuts at: 6631 6631 Size: 10507 SalI G'TCGA_C Cuts at: 1936 4802 5855 7849 1936 Size: 2866 1053 1994 4594 Fragments arranged by size: 4594 2866 1994 1053 SanDI GG'GwC_CC Cuts at: 6448 6448 Size: 10507 SapI GCTCTTCn'nnn_ Cuts at: 4888 6770 4888 Size: 1882 8625 SbfI CC_TGCA'GG Cuts at: 7859 7859 Size: 10507 ScaI AGT'ACT Cuts at: 4935 5151 8755 10467 4935 Size: 216 3604 1712 4975 Fragments arranged by size: 4975 3604 1712 216 SexAI A'CCwGG_T Cuts at: 6819 7300 6819 Size: 481 10026 SfcI C'TryA_G Cuts at: 826 1045 4037 5450 5646 5652 6038 6886 7216 Size: 219 2992 1413 196 6 386 848 330 Cuts at: 7216 7246 7322 7329 7664 7855 8990 9671 9862 Size: 30 76 7 335 191 1135 681 191 Cuts at: 9862 826 Size: 1471 Fragments arranged by size: 2992 1471 1413 1135 848 681 386 335 330 219 196 191 191 76 30 7 6 SfiI GGCCn_nnn'nGGCC Cuts at: 2174 7561 2174 Size: 5387 5120 SfoI GGC'GCC Cuts at: 2021 2021 Size: 10507 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10507 SgrAI Cr'CCGG_yG Cuts at: 1700 4667 1700 Size: 2967 7540 SmaI CCC'GGG Cuts at: 1872 2079 2130 7834 1872 Size: 207 51 5704 4545 Fragments arranged by size: 5704 4545 207 51 SmlI C'TyrA_G Cuts at: 820 1017 1943 3438 4076 4184 4268 5305 5407 Size: 197 926 1495 638 108 84 1037 102 Cuts at: 5407 8611 9512 9774 820 Size: 3204 901 262 1553 Fragments arranged by size: 3204 1553 1495 1037 926 901 638 262 197 108 102 84 SnaBI TAC'GTA Cuts at: 493 8298 493 Size: 7805 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10507 SphI G_CATG'C Cuts at: 1132 7865 1132 Size: 6733 3774 SspI AAT'ATT Cuts at: 5 52 8431 5 Size: 47 8379 2081 Fragments arranged by size: 8379 2081 47 StuI AGG'CCT Cuts at: 1517 5217 7367 1517 Size: 3700 2150 4657 Fragments arranged by size: 4657 3700 2150 StyI C'CwwG_G Cuts at: 513 1065 2040 2232 2377 2601 2913 3495 3999 Size: 552 975 192 145 224 312 582 504 Cuts at: 3999 4157 7607 7946 513 Size: 158 3450 339 3074 Fragments arranged by size: 3450 3074 975 582 552 504 339 312 224 192 158 145 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2691 3670 4633 4697 5213 6537 7214 Size: 1255 1111 979 963 64 516 1324 677 Cuts at: 7214 8534 8551 8704 8889 9334 325 Size: 1320 17 153 185 445 1498 Fragments arranged by size: 1498 1324 1320 1255 1111 979 963 677 516 445 185 153 64 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2396 4908 4933 Size: 274 80 33 51 1443 419 2512 25 Cuts at: 4933 5149 7020 8753 10465 96 Size: 216 1871 1733 1712 138 Fragments arranged by size: 2512 1871 1733 1712 1443 419 274 216 138 80 51 33 25 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3550 3582 4669 4701 5430 5462 6483 6515 10467 Size: 32 1087 32 729 32 1021 32 3952 Cuts at: 10467 10499 3550 Size: 32 3558 Fragments arranged by size: 3952 3558 1087 1021 729 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10507 XbaI T'CTAG_A Cuts at: 3488 4456 7843 3488 Size: 968 3387 6152 Fragments arranged by size: 6152 3387 968 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2416 2496 2512 2943 3313 2416 Size: 80 16 431 370 9610 Fragments arranged by size: 9610 431 370 80 16 XhoI C'TCGA_G Cuts at: 1943 4184 1943 Size: 2241 8266 XmaI C'CCGG_G Cuts at: 1870 2077 2128 7832 1870 Size: 207 51 5704 4545 Fragments arranged by size: 5704 4545 207 51 XmnI GAAnn'nnTTC Cuts at: 5565 6059 6235 8636 5565 Size: 494 176 2401 7436 Fragments arranged by size: 7436 2401 494 176 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8315 276 Size: 53 83 186 936 6781 2468 Fragments arranged by size: 6781 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BstZ17I Bsu36I FspAI NruI NsiI PacI PspXI PsrI RsrII SrfI SwaI