################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AccIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BbeI BbrPI BbvCI BglI BglII BlnI BsaAI BsaBI BseAI BspDI BspEI BssHII Bst1107I BstBI BstZ17I ClaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV FseI FspI KasI KspI MamI MluI MroI NarI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI PvuI SacI SacII SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AclI 2445 AACGTT AA^CGTT AcyI 4432 GGCGTC G[GA]^CG[CT]C AflII 783 CTTAAG C^TTAAG AflIII 3985 ACATGT A^C[GA][CT]GT AocI 4048 CCTGAGG CC^T[ACGT]AGG AspHI 5022 GAGCAC G[AT]GC[AT]^C BamHI 1081 GGATCC G^GATCC BcgI 4559 GCAAGGAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 783 CTTAAG C^TTAAG BlpI 3022 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3022 GCTGAGC GC^T[ACGT]AGC BsaHI 4432 GGCGTC G[GA]^CG[CT]C BsiEI 1030 CGACCG CG[GA][CT]^CG BsiHKAI 5022 GAGCAC G[AT]GC[AT]^C BsiWI 4830 CGTACG C^GTACG BspHI 981 TCATGA T^CATGA BspLU11I 3985 ACATGT A^CATGT BsrBI 3025 GAGCGG CCGCTC(-3/-3) BssSI 1352 CACGAG CACGAG(-5/-1) BstEII 1109 GGTCACC G^GT[ACGT]ACC BstPI 1109 GGTCACC G^GT[ACGT]ACC Bsu36I 4048 CCTGAGG CC^T[ACGT]AGG CelII 3022 GCTGAGC GC^T[ACGT]AGC CpoI 504 CGGACCG CG^G[AT]CCG DrdI 4426 GACTGTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 4048 CCTGAGG CC^T[ACGT]AGG EcoO65I 1109 GGTCACC G^GT[ACGT]ACC EcoRI 2254 GAATTC G^AATTC EcoT22I 1091 ATGCAT ATGCA^T Hin1I 4432 GGCGTC G[GA]^CG[CT]C NaeI 3152 GCCGGC GCC^GGC NgoMIV 3152 GCCGGC G^CCGGC NheI 3043 GCTAGC G^CTAGC NsiI 1091 ATGCAT ATGCA^T Psp1406I 2445 AACGTT AA^CGTT RcaI 981 TCATGA T^CATGA RsrII 504 CGGACCG CG^G[AT]CCG SalI 4435 GTCGAC G^TCGAC SphI 5198 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 1444 GGTACC G^GTACC 2153 GGTACC AccI 4435 GTCGAC GT^[AC][GT]AC 4987 GTAGAC AccII 472 CGCG CG^CG 3760 CGCG Asp700I 1089 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3310 GAAAATATTC Asp718I 1444 GGTACC G^GTACC 2153 GGTACC AvaI 1686 CCCGAG C^[CT]CG[GA]G 5353 CCCGAG BalI 3742 TGGCCA TGG^CCA 4821 TGGCCA BciVI 2521 GGATAC GTATCC(6/5) 558 GTATCC BcnI 3144 CCCGG CC^[GC]GG 6151 CCCGG BsaWI 1501 TCCGGT [AT]^CCGG[AT] 3799 ACCGGA BsoBI 1686 CCCGAG C^[CT]CG[GA]G 5353 CCCGAG Bsp1407I 3818 TGTACA T^GTACA 6140 TGTACA BsrFI 2150 GCCGGT [GA]^CCGG[CT] 3152 GCCGGC BsrGI 3818 TGTACA T^GTACA 6140 TGTACA BstUI 472 CGCG CG^CG 3760 CGCG BstXI 1755 CCACTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5333 CCATGCAGCTGG BstYI 1081 GGATCC [GA]^GATC[CT] 4140 AGATCC Cfr10I 2150 GCCGGT [GA]^CCGG[CT] 3152 GCCGGC DraI 1840 TTTAAA TTT^AAA 3805 TTTAAA DraIII 1352 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG 4013 CACCAGGTG HpaI 1279 GTTAAC GTT^AAC 5466 GTTAAC KpnI 1444 GGTACC GGTAC^C 2153 GGTACC MfeI 341 CAATTG C^AATTG 1847 CAATTG MflI 1081 GGATCC [GA]^GATC[CT] 4140 AGATCC MluNI 3742 TGGCCA TGG^CCA 4821 TGGCCA MscI 3742 TGGCCA TGG^CCA 4821 TGGCCA MunI 341 CAATTG C^AATTG 1847 CAATTG MvnI 472 CGCG CG^CG 3760 CGCG NciI 3144 CCCGG CC^[GC]GG 6151 CCCGG NdeI 886 CATATG CA^TATG 6095 CATATG PflMI 117 CCATGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5289 CCAGCAGCTGG PshAI 1131 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4735 GACCAAAGTC SspBI 3818 TGTACA T^GTACA 6140 TGTACA Van91I 117 CCATGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5289 CCAGCAGCTGG XbaI 283 TCTAGA T^CTAGA 2641 TCTAGA XhoII 1081 GGATCC [GA]^GATC[CT] 4140 AGATCC XmnI 1089 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3310 GAAAATATTC ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BanI BanII BbsI BbvI BclI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmBI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI